| 质粒名称: | pSL1999 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002463 |
| 产品用途: | Mammalian PhCMV-driven TetR-nMag (Magnet) expression vector (PhCMV-TetR-nMag-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | TetR-nMag |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1999
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1999载体质粒基本信息
pSL1999质粒图谱载体图谱和pSL1999载体序列质粒序列多克隆位点信息
pSL1999质粒载体简介
pSL1999质粒序列载体序列
LOCUS pSL1999 6495 bp DNA circular SYN 09-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6495)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 31, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6495
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 880..937
/label=oLZ169 TetR F
primer_bind 881..926
/label=oSCL1081
misc_feature 912..1529
/label=tetr
primer_bind complement(1510..1549)
/label=oLZ170 TetR R
primer_bind complement(1510..1529)
/label=oLZ166 TetR R
primer_bind complement(1528..1543)
/label=oSCL3103
CDS 1530..1559
/codon_start=1
/label=linker
/translation="ASGSGGGGDV"
primer_bind complement(1540..1565)
/label=oSCL1119
primer_bind complement(1540..1559)
/label=oSCL075
primer_bind 1546..1585
/label=oSCL3104
misc_feature 1561..1564
/label=linker
misc_feature 1566..2048
/label=nMag
primer_bind 1566..1585
/label=oLZ139 nMag-L F
primer_bind 1566..1585
/label=oSCL3102
primer_bind complement(2029..2071)
/label=oSCL3105
primer_bind complement(2029..2048)
/label=oSCL3103
terminator 2092..2319
/label=bGH PA
rep_origin 2365..2793
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2807..3136
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 3203..3997
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4171..4292
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4337..4357)
/label=M13-rev
misc_binding complement(4363..4385)
/label=LacO
promoter complement(4389..4419)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4434..4455
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4743..5328)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5499..6359)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6360..6464)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagg
901 aattcgccac catgtctaga ctggacaaga gcaaagtcat aaactctgct ctggaattac
961 tcaatgaagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag ctgggagttg
1021 agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat gccctggcaa
1081 tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag tcatggcaag
1141 actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat cgcgacgggg
1201 ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaaaccctg gaaaatcagc
1261 tcgcgttcct gtgtcagcaa ggcttctccc tggagaacgc actgtacgct ctgtccgccg
1321 tgggccactt tacactgggc tgcgtattgg aggatcagga gcatcaagta gcaaaagagg
1381 aaagagagac acctaccacc gattctatgc ccccacttct gagacaagca attgagctgt
1441 tcgaccatca gggagccgaa cctgccttcc ttttcggcct ggaactaatc atatgtggcc
1501 tggagaaaca gctaaagtgc gaaagcggcg ccagcggaag tggcggagga ggcgacgtcg
1561 gatcccatac tctttatgcc cccggtggat atgacattat gggatatctg gaccagatcg
1621 gcaaccggcc aaacccgcag gtggaactgg gccccgtgga tacatcctgc gccttgattc
1681 tttgtgacct gaaacagaaa gacaccccga tagtttacgc gagtgaagcc ttcctctaca
1741 tgacaggtta cagcaacgca gaggtgctgg gccggaattg ccggtttctg caaagccctg
1801 acggcatggt gaagcccaag agcacccgga agtacgtgga tagtaacaca atcaatacta
1861 tgcgcaaggc aatcgacagg aatgccgagg tgcaggttga agtagtcaat tttaaaaaga
1921 atggacagcg atttgttaat ttcctgacta tgatacctgt tagggacgaa acaggcgagt
1981 atcgatactc tatgggattc cagtgcgaaa cagaaggcgg aagcggtggc gtgcccaaga
2041 agaagaggta actcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc
2101 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
2161 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
2221 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
2281 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
2341 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
2401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
2461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
2521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
2581 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
2641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
2701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
2761 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
2821 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
2881 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
2941 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
3001 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
3061 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
3121 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga
3181 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc
3241 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga
3301 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct
3361 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac
3421 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct
3481 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt
3541 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt
3601 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt
3661 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag
3721 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt
3781 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg
3841 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg
3901 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg
3961 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg
4021 accgaccaag cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat
4081 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
4141 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
4201 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
4261 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
4321 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
4381 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
4441 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
4501 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
4561 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
4621 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
4681 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
4741 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
4801 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
4861 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
4921 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
4981 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
5041 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
5101 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
5161 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
5221 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
5281 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
5341 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
5401 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
5461 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
5521 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
5581 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
5641 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
5701 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
5761 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
5821 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
5881 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
5941 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
6001 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
6061 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
6121 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
6181 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
6241 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
6301 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
6361 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
6421 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
6481 gtgccacctg acgtc
//
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