pCB578

LOCUS       pCB578               11446 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and
            a repeat-spacer-repeat array.
ACCESSION   .
VERSION     .
KEYWORDS    pCB578
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11446)
  AUTHORS   Leenay RT, Vento JM, Shah M, Martino ME, Leulier F, Beisel CL
  TITLE     Genome Editing with CRISPR-Cas9 in Lactobacillus plantarum Revealed 
            That Editing Outcomes Can Vary Across Strains and Between Methods.
  JOURNAL   Biotechnol J. 2019 Mar;14(3):e1700583. doi: 10.1002/biot.201700583. 
            Epub 2018 Sep 20.
  PUBMED    30156038
REFERENCE   2  (bases 1 to 11446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11446
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_RNA        complement(3024..3102)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     CDS             3398..7504
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     rep_origin      8046..8634
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8535..8554
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8788..8805
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8892..8911
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8892..8910
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   9479..9514
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   9545..9580
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
ORIGIN
        1 aatcccaaat gagccaacag aaccagaacc agaaacagaa tcagaacaag taacattgga
       61 tttagaaatg gaagaagaaa aaagcaatga cttcgtgtga ataatgcacg aaatcgttgc
      121 ttattttttt taaaagcggt atactagata taacgaaaca acgaactgaa tagaaacgaa
      181 aaaagagcca tgacacattt ataaaatgtt tgacgacatt ttataaatgc atagcccgat
      241 aagattgcca aaccaacgct tatcagttag tcagatgaac tcttccctcg taagaagtta
      301 tttaattaac tttgtttgaa gacggtatat aaccgtacta tcattatata gggaaatcag
      361 agagttttca agtatctaag ctactgaatt taagaattgt taagcaatca atcggaaatc
      421 gtttgattgc tttttttgta ttcatttata gaaggtggag tttgtatgaa tcatgatgaa
      481 tgtaaaactt atataaaaaa tagtttattg gagataagaa aattagcaaa tatctataca
      541 ctagaaacgt ttaagaaaga gttagaaaag agaaatatct acttagaaac aaaatcagat
      601 aagtattttt cttcggaggg ggaagattat atatataagt taatagaaaa taacaaaata
      661 atttattcga ttagtggaaa aaaattgact tataaaggaa aaaaatcttt ttcaaaacat
      721 gcaatattga aacagttgaa tgaaaaagca aaccaagtta attaaacaac ctattttata
      781 ggatttatag gaaaggagaa cagctgaatg aatatccctt ttgttgtaga aactgtgctt
      841 catgacggct tgttaaagta caaatttaaa aatagtaaaa ttcgctcaat cactaccaag
      901 ccaggtaaaa gcaaaggggc catttttgcg tatcgctcaa aatcaagcat gattggcggt
      961 cgtggtgttg ttctgacttc cgaggaagcg attcaagaaa atcaagatac atttacacat
     1021 tggacaccca acgtttatcg ttatggaacg tatgcagacg aaaaccgttc atacacgaaa
     1081 ggacattctg aaaacaattt aagacaaatc aataccttct ttattgattt tgatattcac
     1141 acggcaaaag aaactatttc agcaagcgat attttaacaa ccgctattga tttaggtttt
     1201 atgcctacta tgattatcaa atctgataaa ggttatcaag catattttgt tttagaaacg
     1261 ccagtctatg tgacttcaaa atcagaattt aaatctgtca aagcagccaa aataatttcg
     1321 caaaatatcc gagaatattt tggaaagtct ttgccagttg atctaacgtg taatcatttt
     1381 ggtattgctc gcataccaag aacggacaat gtagaatttt ttgatcctaa ttaccgttat
     1441 tctttcaaag aatggcaaga ttggtctttc aaacaaacag ataataaggg ccttactcgt
     1501 tcaagtctaa cggttttaag cggtacagaa ggcaaaaaac aagtagatga accctggttt
     1561 aatctcttat tgcacgaaac gaaattttca ggagaaaagg gtttaatagg gcgtaataac
     1621 gtcatgttta ccctctcttt agcctacttt agttcaggct attcaatcga aacgtgcgaa
     1681 tataatatgt ttgagtttaa taatcgatta gatcaaccct tagaagaaaa agaagtaatc
     1741 aaaattgtta gaagtgccta ttcagaaaac tatcaagggg ctaataggga atacattacc
     1801 attctttgca aagcttgggt atcaagtgat ttaaccagta aagatttatt tgtccgtcaa
     1861 gggtggttta aattcaagaa aaaaagaagc gaacgtcaac gtgttcattt gtcagaatgg
     1921 aaagaagatt taatggctta tattagcgaa aaaagcgatg tatacaagcc ttatttagtg
     1981 acgaccaaaa aagagattag agaagtgcta ggcattcctg aacggacatt agataaattg
     2041 ctgaaggtac tgaaggcgaa tcaggaaatt ttctttaaga tcaaaccagg aagaaatggt
     2101 ggcattcaac ttgctagtgt taaatcattg ttgctatcga tcattaaagt aaaaaaagaa
     2161 gaaaaagaaa gctatataaa ggcgctgaca aattcttttg acttagagca tacattcatt
     2221 caagagactt taaacaagct agcagaacgc cctaaaacgg acacacaact cgatttgttt
     2281 agctatgata caggctgaaa ataaaacccg cactatgcca ttacatttat atctatgata
     2341 cgtgtttgtt ttttctttgc tgtttagcga atgattagca gaaatataca gagtaagatt
     2401 ttaattaatt attaggggga gaaggagaga gtagcccgga aacttttagt tggcttggac
     2461 tgaacgaagt gagggaaagg ctactaaaac gtcgaggggc agtgagagcg aagcgaacac
     2521 ttgatttttt aattttctat cttttatagg tcattagagt atacttattt gtcctataaa
     2581 ctatttagca gcataataga tttattgaat aggtcattta agttgagcat attagaggag
     2641 gaaaatcttg gagaaatatt tgaagaaccc gattacatgg attggattag ttcttgtggt
     2701 tacgtggttt ttaactaaaa gtagtgaatt tttgattttt ggtgtgtgtg tcttgttgtt
     2761 agtatttgct agtcaaagtg attaaataga attctcatgt ttgacagctt atcatcggag
     2821 ctctgatgat aagcggtcca aacatgagaa ttccttacga aatcatcctg tggagcttag
     2881 taggtttagc aagatggcag cgcctaaatg tagaatgata aaaggattaa gagattaatt
     2941 tccctaaaaa tgataaaaca agcgttttga aagcgcttgt ttttttggtt tgcagtcaga
     3001 gtagaataga agtatcaaaa aaagcaccga ctcggtgcca ctttttcaag ttgataacgg
     3061 actagcctta ttttaacttg ctatgctgtt ttgaatggtt ccaacaagat tattttataa
     3121 cttttataac aaataatcaa ggagaaattc aaagaaattt atcagccata aaacaatact
     3181 taatactata gaatgataac aaaataaact actttttaaa agaattttgt gttataatct
     3241 atttattatt aagtattggg taatattttt tgaagagata ttttgaaaaa gaaaaattaa
     3301 agcatattaa actaatttcg gaggtcatta aaactattat tgaaatcatc aaactcatta
     3361 tggatttaat ttaaactttt tattttagga ggcaaaaatg gataagaaat actcaatagg
     3421 cttagatatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc
     3481 gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat
     3541 aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc
     3601 tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa
     3661 tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga
     3721 agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta
     3781 tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa
     3841 agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt
     3901 tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt
     3961 ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc
     4021 taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca
     4081 gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt
     4141 gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa
     4201 agatacttac gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga
     4261 tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt
     4321 aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca
     4381 tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa
     4441 agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag
     4501 ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga
     4561 attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg
     4621 ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga
     4681 cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat
     4741 tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa
     4801 gtctgaagaa acaattaccc catggaattt tgaagaagtt gtcgataaag gtgcttcagc
     4861 tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact
     4921 accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa
     4981 atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat
     5041 tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta
     5101 tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa
     5161 tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga
     5221 taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga
     5281 tagggagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat
     5341 gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa
     5401 tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt
     5461 tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat
     5521 tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc
     5581 tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt
     5641 caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca
     5701 gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat
     5761 caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa
     5821 tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt
     5881 agatattaat cgtttaagtg attatgatgt cgatcacatt gttccacaaa gtttccttaa
     5941 agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga
     6001 taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa
     6061 cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt
     6121 gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac
     6181 taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa
     6241 acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa
     6301 agatttccaa ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta
     6361 tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt
     6421 tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga
     6481 aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact tcttcaaaac
     6541 agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga
     6601 aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc
     6661 catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga
     6721 gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc
     6781 aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa
     6841 ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat
     6901 tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa
     6961 ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa
     7021 cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct
     7081 gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag
     7141 tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga
     7201 gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga
     7261 taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa
     7321 tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga
     7381 tacaacaatt gatcgtaaac gatatacgtc tacaaaagaa gttttagatg ccactcttat
     7441 ccatcaatcc atcactggtc tttatgaaac acgtattgat ttgagtcagc taggaggtga
     7501 ctgaaaaagg tggggcagaa gttatcctaa aaataaaaaa gtaatttagt aatctctaag
     7561 gattactttt tttgtttcgg caaactatta actggcgaac tacttactct agcttcccgg
     7621 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7681 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7741 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7801 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7861 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7921 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7981 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     8041 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     8101 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     8161 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8221 cacttcaaga actctgtagc accgcctaca tacctcgctt tgctaatcct gttaccagtg
     8281 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8341 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8401 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8461 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8521 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8581 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8641 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8701 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8761 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8821 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag gttaacctgg
     8881 cttatcgaaa ttaatacgac tcactatagg gagaccggcc tcgagaagct cgcgttatcg
     8941 gtcctttaat tggtggacaa atttacatta gtctcggaca ttctgctccc gcccttatgg
     9001 gatttatctt ccttattctc gctttgattg ttctatcgaa agcgaaatca aacgattaat
     9061 ttataaataa aaaaaccacc tctaaaaggt gatttttatt tataaattac aatttttcag
     9121 caatatcagt aattgcttta tcaactgctg ctttttggct atcaatcaaa gcaacacgtg
     9181 ctgtaatttg tttaattgcc atttcaattg aacgtttcaa gccttggttt tctaattttg
     9241 gttcaaagaa agcttgagct ctcgagtcag gggtaccgat caggaaatta aaattggtct
     9301 catataactg aattattttc ggaaaataaa gggaatctgt ttacaaacat taccagtatc
     9361 gatataatac ttaaggattc ttcgaaaatt gactactttg tcttttccag aagatgtagt
     9421 ataataacac ttagaaatgc attgatgcga aattgatgta attcttgaaa acatgtacgt
     9481 tttagagcta tgctgttttg aatggtccca aaacttcgat cgacgaacgt gtcactaaag
     9541 ccttgtttta gagctatgct gttttgaatg gtcccaaaac atgcggccgc gccacaccgc
     9601 aaaccccgcc cctgacaggg cggggtttcg ccgcgatcaa ctcattgcag acatttgcag
     9661 tacccatggt gagtgcctcc ttataattta ttttgtagtt ccttcgaacg aaatcattgt
     9721 atctaacaaa cttcagaatt taatcagagc cgtttattat gctcgcgtta tcgacaataa
     9781 tattattacc aatactttct caagatagaa ttaagactgt tttagatttg ttaatgtttc
     9841 tattgtcagt atagttataa gactagatct gatccgtagc ggttttcaaa atttgcaacc
     9901 aggaatgaat tactatccct tttatcaaga agcgcacaaa aagaaaaacg aaatgataca
     9961 ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg
    10021 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa
    10081 ataagactta gaagcaaact taagagtgtg ttgatagtgc agtatcttaa aattttgtat
    10141 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gtgattacat
    10201 gaacaaaaat ataaaatatt ctcaaaactt tttaacgagt gaaaaagtac tcaaccaaat
    10261 aataaaacaa ttgaatttaa aagaaaccga taccgtttac gaaattggaa caggtaaagg
    10321 gcatttaacg acgaaactgg ctaaaataag taaacaggta acgtctattg aattagacag
    10381 tcatctattc aacttatcgt cagaaaaatt aaaactgaat actcgtgtca ctttaattca
    10441 ccaagatatt ctacagtttc aattccctaa caaacagagg tataaaattg ttgggagtat
    10501 tccttaccat ttaagcacac aaattattaa aaaagtggtt tttgaaagcc atgcgtctga
    10561 catctatctg attgttgaag aaggattcta caagcgtacc ttggatattc accgaacact
    10621 agggttgctc ttgcacactc aagtctcgat tcagcaattg cttaagctgc cagcggaatg
    10681 ctttcatcct aaaccaaaag taaacagtgt cttaataaaa cttacccgcc ataccacaga
    10741 tgttccagat aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga
    10801 atatcgtcaa ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa
    10861 caatttaagt accgttactt atgagcaagt attgtctatt tttaatagtt atctattatt
    10921 taacgggagg aaataattct atgagtcgct tttgtaaatt tggaaagtta cacgttacta
    10981 aagggaatgt agataaatta ttaggtatac tactgacagc ttccaaggag ctaaagaggt
    11041 ccctagcgct tagaatcgct ttaggaaaca cgatccagtc caataatcgt cgataaaaac
    11101 ttttgaaaaa ggttggtgaa attacctact tttggaataa tcacaaatca caagtgatta
    11161 atcacaaatc acaagtgatt aatcacttgt ttattaagat attaaaagct ataatttaaa
    11221 taaagcgtga attttattac acaaaaagag gggggagaaa cttggaacta gcatttagag
    11281 aaagcttaaa aaagatgaga ggtaccaaat caaaagaaaa attctcccaa gaattagaaa
    11341 tgagtagatc aaattattca cgaatagaat caggaaaatc agatccaacc ataaaaacac
    11401 tagaacaaat tgcaaagtta actaactcaa cgctagtagt ggattt
//