| 质粒名称: |
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001915 |
| 产品用途: | 3' AAVLINK plasmid for YE1-BE4max expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | YE1-BE4max |
| 物种来源: | 未知 |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-YE1-BE4max 6769 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6769)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6769
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..2944
/codon_start=1
/product="nickase mutant of the Cas9 endonuclease from the
Streptococcus pyogenes Type II CRISPR/Cas system"
/label=Cas9(D10A)
/note="generates RNA-guided single strand nicks in DNA due
to the D10A mutation in the RuvC catalytic domain"
/translation="AERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDEND
KLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLES
EFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIET
NGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKK
DWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLE
AKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHY
EKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKP
IREQAENIIHLFTLTNLGAPAAFKYFNTTIDRKRYTSTKEVLDATLIHQSITGLYETRI
DLSQLGGD"
CDS 2294..2944
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="GKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII
KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQK
QLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFT
LTNLGAPAAFKYFNTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
CDS 2975..3223
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
misc_feature 3051..3056
/label=SDS
CDS 3254..3502
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 3545..3565
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 3569..4000
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3713..3718
/label=SDS
repeat_region 4043..4172
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
rep_origin complement(4234..4822)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4993..5853)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5854..5958)
/gene="bla"
/label=AmpR promoter
rep_origin complement(6240..6695)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggccg agagaggcgg
1561 cctgagcgaa ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat
1621 cacaaagcac gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga
1681 caagctgatc cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg
1741 gaaggatttc cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc
1801 ctacctgaac gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga
1861 gttcgtgtac ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca
1921 ggaaatcggc aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa
1981 gaccgagatt accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg
2041 cgaaaccggg gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct
2101 gagcatgccc caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa
2161 agagtctatc ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga
2221 ccctaagaag tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc
2281 caaagtggaa aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac
2341 catcatggaa agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta
2401 caaagaagtg aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga
2461 aaacggccgg aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc
2521 cctgccctcc aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg
2581 ctcccccgag gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga
2641 cgagatcatc gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct
2701 ggacaaagtg ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga
2761 gaatatcatc cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt
2821 taacaccacc atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct
2881 gatccaccag agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg
2941 tgacagcggc gggagcggcg ggagcggggg gagcactaat ctgagcgaca tcattgagaa
3001 ggagactggg aaacagctgg tcattcagga gtccatcctg atgctgcctg aggaggtgga
3061 ggaagtgatc ggcaacaagc cagagtctga catcctggtg cacaccgcct acgacgagtc
3121 cacagatgag aatgtgatgc tgctgacctc tgacgccccc gagtataagc cttgggccct
3181 ggtcatccag gattctaacg gcgagaataa gatcaagatg ctgagcggag gatccggagg
3241 atctggaggc agcaccaacc tgtctgacat catcgagaag gagacaggca agcagctggt
3301 catccaggag agcatcctga tgctgcccga agaagtcgaa gaagtgatcg gaaacaagcc
3361 tgagagcgat atcctggtcc ataccgccta cgacgagagt accgacgaaa atgtgatgct
3421 gctgacatcc gacgccccag agtataagcc ctgggctctg gtcatccagg attccaacgg
3481 agagaacaaa atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt
3541 cgagcccaag aagaagagga aagtctaact cgagataatc aacctctgga ttacaaaatt
3601 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
3661 gctttaatgc ctttgcatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
3721 tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
3781 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg tgaaatttgt
3841 gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg atgctattgc
3901 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
3961 tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag accggtgtgg
4021 cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac tccctctctg
4081 cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggcggcctc
4141 agtgagcgag cgagcgcgca gctgcctgca ggacatgtga gcaaaaggcc agcaaaaggc
4201 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
4261 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4321 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
4381 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4441 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4501 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
4561 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
4621 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
4681 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4741 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
4801 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
4861 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
4921 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
4981 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
5041 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
5101 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
5161 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
5221 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
5281 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
5341 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
5401 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
5461 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
5521 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
5581 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
5641 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
5701 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
5761 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
5821 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
5881 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
5941 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
6001 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
6061 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
6121 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
6181 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccataa
6241 aattgtaaac gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
6301 ttttaaccaa tagaccgaaa tcggcaaaat cccttataaa tcaaaagaat agcccgagat
6361 agagttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
6421 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccaa
6481 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
6541 ccgatttaga gcttgacggg gaaagccggc gagcgtggcg agaaaggaag ggaagaaagc
6601 gaaaggagcg ggcgctaagg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
6661 acccgccgcg cttaatgcgc cgctacaggg cgcgtactat ggttgctttg acgtatgcgg
6721 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcc
//
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