| 质粒名称: | pBAD-6xHis-mCardinal |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000099 |
| 产品用途: | Expresses mCardinal in bacteria for purification |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD |
| 表达系统: | 细菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mCardinal |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
买家导航
pBAD-6xHis-mCardinal载体质粒基本信息
pBAD-6xHis-mCardinal质粒图谱载体图谱和pBAD-6xHis-mCardinal载体序列质粒序列多克隆位点信息
pBAD-6xHis-mCardinal质粒载体简介
pBAD-6xHis-mCardinal质粒序列载体序列
LOCUS pBAD-6xHis-mCardinal 4734 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4734)
AUTHORS Kiryl Piatkevich
TITLE Direct Submission
JOURNAL Exported Apr 14, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4734
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 120..285
/gene="araBAD"
/label=araBAD promoter
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction"
primer_bind 194..217
/label=araBAD-F
CDS 319..327
/codon_start=1
/label=Start
/translation="MGS"
primer_bind 321..340
/label=assembly-F
primer_bind 324..361
/label=mCardinal-F
CDS 328..345
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 355..1086
/codon_start=1
/product="far-red fluorescent protein derived from
mNeptune, with further red-shifted spectra"
/label=mCardinal
/translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ
RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD
MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV
ARYCDLPSKLGHKLNGMDELYK"
regulatory 355..358
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1065..1120)
/label=mCardinal-R
primer_bind complement(1090..1113)
/label=assembly-R
terminator 1305..1391
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind complement(1320..1345)
/label=rrnB-T1-R
terminator 1483..1510
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 1529..1620
/gene="bla"
/label=AmpR promoter
CDS 1621..2481
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2652..3240
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 3426..3566
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(3830..4708)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat ggggagccac catcaccatc accatggcct cgagatggtg
361 agcaagggcg aggagctgat caaggagaac atgcacatga agctgtacat ggaaggcacc
421 gtgaacaacc accacttcaa gtgcaccacc gaaggggagg gcaagcccta cgagggcacc
481 cagacccaga ggattaaggt ggtggaggga ggccccctgc cgttcgcatt cgacatcctg
541 gccacctgct ttatgtacgg gagcaagacc ttcatcaacc acacccaggg catccccgat
601 ttctttaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
661 gggggcgtgc ttaccgttac ccaggacacc agcctccagg acggctgctt gatctacaac
721 gtcaagctca gaggggtgaa cttcccatcc aacggccctg tgatgcagaa gaaaacactc
781 ggctgggagg ccaccaccga gaccctgtac cccgctgacg gcggcctgga aggcagatgc
841 gacatggccc tgaagctcgt gggcgggggc cacctgcact gcaacctgaa gaccacatac
901 agatccaaga aacccgctaa gaacctcaag atgcccggcg tctactttgt ggaccgcaga
961 ctggaaagaa tcaaggaggc cgacaatgag acctacgtcg agcagcacga ggtggctgtg
1021 gccagatact gcgacctccc tagcaaactg gggcacaaac ttaatggcat ggacgagctg
1081 tacaagtaag aattcgaagc ttggctgttt tggcggatga gagaagattt tcagcctgat
1141 acagattaaa tcagaacgca gaagcggtct gataaaacag aatttgcctg gcggcagtag
1201 cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg
1261 tagtgtgggg tctccccatg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
1321 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
1381 gtaggacaaa tccgccggga gcggatttga acgttgcgaa gcaacggccc ggagggtggc
1441 gggcaggacg cccgccataa actgccaggc atcaaattaa gcagaaggcc atcctgacgg
1501 atggcctttt tgcgtttcta caaactcttt tgtttatttt tctaaataca ttcaaatatg
1561 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
1621 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
1681 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
1741 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
1801 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
1861 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
1921 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
1981 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
2041 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
2101 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
2161 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
2221 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
2281 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
2341 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
2401 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
2461 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
2521 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
2581 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
2641 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
2701 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
2761 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
2821 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
2881 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
2941 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
3001 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
3061 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
3121 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
3181 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
3241 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
3301 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
3361 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
3421 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
3481 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
3541 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
3601 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
3661 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagcag atcaattcgc
3721 gcgcgaaggc gaagcggcat gcataatgtg cctgtcaaat ggacgaagca gggattctgc
3781 aaaccctatg ctactccgtc aagccgtcaa ttgtctgatt cgttaccaat tatgacaact
3841 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
3901 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
3961 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
4021 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
4081 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
4141 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
4201 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
4261 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
4321 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
4381 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
4441 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
4501 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
4561 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
4621 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
4681 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agag
//
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