pLYL188

LOCUS      pLYL188             8060 bp DNA     circular SYN 15-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8060)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8060
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     213..241
                     /label=OGT82-1-F
     misc_feature    235..818
                     /label=P_CMV
                     /label=nonstandard type: promoter_const
     misc_feature    235..614
                     /label=P_CMV_enhancer
                     /label=nonstandard type: promoter_element
     misc_feature    615..818
                     /label=P_CMV_IE
                     /label=nonstandard type: promoter_element
     promoter        863..882
                     /label=T7 promoter
     primer_bind     881..908
                     /label=olyl401
     primer_bind     897..949
                     /label=olyl502
     primer_bind     897..922
                     /label=olyl479
     misc_feature    923..1627
                     /label=Ritux LC
     primer_bind     complement(989..1035)
                     /label=olyl482
     primer_bind     1014..1035
                     /label=olyl483
     primer_bind     complement(1606..1664)
                     /label=olyl503
     CDS             1628..1642
                     /codon_start=1
                     /label=FCS
                     /translation="KRRKR"
     CDS             1628
                     /codon_start=1
                     /label=fcs
                     /translation=""
     primer_bind     1644..1692
                     /label=olyl505
     CDS             1652..1705
                     /codon_start=1
                     /label=T2A
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     1673..1729
                     /label=olyl509
     primer_bind     1673..1729
                     /label=olyl504
     misc_feature    1706..3109
                     /label=Ritux HC
     primer_bind     complement(2353..2392)
                     /label=olyl480
     primer_bind     2375..2395
                     /label=olyl481
     primer_bind     complement(3085..3142)
                     /label=olyl506
     primer_bind     3110..3158
                     /label=olyl345
     CDS             3110..3121
                     /codon_start=1
                     /label=fcs
                     /translation="RIKR"
     misc_feature    3110
                     /label=FURIN cleavage
     primer_bind     3117..3158
                     /label=olyl339
     primer_bind     complement(3118..3156)
                     /label=olyl507
     misc_feature    3122..3136
                     /label=GS Linker
     primer_bind     3133..3158
                     /label=olyl347
     misc_feature    3143..3621
                     /label=ecDHFR(N18T/A19V)
     primer_bind     complement(3597..3619)
                     /label=olyl402
     primer_bind     complement(3615..3642)
                     /label=olyl340
     terminator      3657..3884
                     /label=bGH PA
     rep_origin      3930..4358
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     misc_feature    4372..4701
                     /label=P_SV40
                     /label=nonstandard type: promoter_const
     rep_origin      4552..4687
                     /label=SV40 ORI
     misc_feature    4552..4629
                     /label=SV40 ORI
     misc_feature    4604..4653
                     /label=P_SV40 TSS-region
     CDS             4768..5562
                     /label=Translation 2136-2930
     misc_feature    4768..5559
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     CDS             4771..5562
                     /label=Kan/neoR
     primer_bind     complement(5902..5922)
                     /label=M13-rev
     misc_binding    complement(5928..5950)
                     /label=LacO
     promoter        complement(5954..5984)
                     /label=lac promoter
     promoter        complement(5955..5984)
                     /label=lac
     rep_origin      complement(6308..6893)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     _ori
     CDS             complement(7064..7924)
                     /label=Translation 5292-4432
     misc_feature    complement(7067..7924)
                     /label=AmpR
                     /label=nonstandard type: resistance
     CDS             complement(7067..7726)
                     /label=AmpR
     promoter        complement(7925..8029)
                     /label=AmpR promoter
     promoter        complement(7966..7994)
                     /label=Amp prom
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttgcca ccatggattt tcaggtgcag attatcagct tcctgctaat
      961 cagtgcttca gtcataatgt ccagagggca aattgttctc tcccagtctc cagcaatcct
     1021 gtctgcatct ccaggggaga aggtcacaat gacttgcagg gccagctcaa gtgtaagtta
     1081 catccactgg ttccagcaga agccaggaag ctcccccaaa ccctggattt atgccacatc
     1141 caacctggct tctggagtcc ctgttcgctt cagtggcagt gggtctggga cttcttactc
     1201 tctcacaatc agcagagtgg aggctgaaga tgctgccact tattactgcc agcagtggac
     1261 tggtaaccca cccacgttcg gaggggggac caagctggaa atcaaacgta cggtggctgc
     1321 accatctgtc ttcatcttcc cgccatctga tgagcagttg aaatctggaa ctgcctctgt
     1381 tgtgtgcctg ctgaataact tctatcccag agaggccaaa gtacagtgga aggtggataa
     1441 cgccctccaa tcgggtaact cccaggagag tgtcacagag caggacagca aggacagcac
     1501 ctacagcctc agcagcaccc tgacgctgag caaagcagac tacgagaaac acaaagtcta
     1561 cgcctgcgaa gtcacccatc agggcctgtc ctcccccgtc acaaagagct tcaacagggg
     1621 agagtgtaag cgccgtaaac gcgctgctgc tgagggcaga ggaagtcttc taacatgcgg
     1681 tgacgtggag gagaatcccg gccctggttg gagcctcatc ttgctcttcc ttgtcgctgt
     1741 tgctacacgt gtcctgtccc aggtacaact gcagcagcct ggggctgagc tggtgaagcc
     1801 tggggcctca gtgaagatgt cctgcaaggc ttctggctac acatttacca gttacaatat
     1861 gcactgggta aaacagacac ctggtcgggg cctggaatgg attggagcta tttatcccgg
     1921 aaatggtgat acttcctaca atcagaagtt caaaggcaag gccacattga ctgcagacaa
     1981 atcctccagc acagcctaca tgcagctcag cagcctgaca tctgaggact ctgcggtcta
     2041 ttactgtgca agatcgactt actacggcgg tgactggtac ttcaatgtct ggggcgcagg
     2101 gaccacggtc accgtgtctg cagccagcac caagggccca tcggtcttcc ccctggcacc
     2161 ctcctccaag agcacctctg ggggcacagc ggccctgggc tgcctggtca aggactactt
     2221 ccccgaaccg gtgacggtgt cgtggaactc aggcgccctg accagcggcg tgcacacctt
     2281 cccggctgtc ctacagtcct caggactcta ctccctcagc agcgtggtga ccgtgccctc
     2341 cagcagcttg ggcacccaga cctacatctg caacgtgaat cacaagccca gcaacaccaa
     2401 ggtggacaag aaagttgagc ccaaatcttg tgacaaaact cacacatgcc caccgtgccc
     2461 agcacctgaa ctcctggggg gaccgtcagt cttcctcttc cccccaaaac ccaaggacac
     2521 cctcatgatc tcccggaccc ctgaggtcac atgcgtggtg gtggacgtga gccacgaaga
     2581 ccctgaggtc aagttcaact ggtacgtgga cggcgtggag gtgcataatg ccaagacaaa
     2641 gccgcgggag gagcagtaca acagcacgta ccgtgtggtc agcgtcctca ccgtcctgca
     2701 ccaggactgg ctgaatggca aggagtacaa gtgcaaggtc tccaacaaag ccctcccagc
     2761 ccccatcgag aaaaccatct ccaaagccaa agggcagccc cgagaaccac aggtgtacac
     2821 cctgccccca tcccgggatg agctgaccaa gaaccaggtc agcctgacct gcctggtcaa
     2881 aggcttctat cccagcgaca tcgccgtgga gtgggagagc aatgggcagc cggagaacaa
     2941 ctacaagacc acgcctcccg tgctggactc cgacggctcc ttcttcctct acagcaagct
     3001 caccgtggac aagagcaggt ggcagcaggg gaacgtcttc tcatgctccg tgatgcatga
     3061 ggctctgcac aaccactaca cgcagaagag cctctccctg tctccgggta ggatcaagag
     3121 gggaggagga tctggagaat tcatgatcag cctgatcgcc gccctggccg tggaccacgt
     3181 gatcggcatg gagaccgtga tgccctggaa cctgcccgcc gacctggcct ggttcaagag
     3241 aaacaccctg aacaagcccg tgatcatggg cagacacacc tgggagagca tcggcagacc
     3301 cctgcccggc agaaagaaca tcatcctgag cagccagccc agcaccgacg acagagtgac
     3361 ctgggtgaag agcgtggacg aggccatcgc cgcctgcggc gacgtgcccg agatcatggt
     3421 gatcggcggc ggcagagtgt acgagcagtt cctgcccaag gcccagaagc tgtacctgac
     3481 ccacatcgac gccgaggtgg agggcgacac ccacttcccc gactacgagc ccgacgactg
     3541 ggagagcgtg ttcagcgagt tccacgacgc cgacgcccag aacagccaca gctactgctt
     3601 cgagatcctg gagagaagat agtctagagg gcccgtttaa acccgctgat cagcctcgac
     3661 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     3721 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     3781 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     3841 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     3901 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     3961 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4021 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4081 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4141 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4201 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4261 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4321 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4381 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4441 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     4501 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     4561 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     4621 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     4681 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     4741 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     4801 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     4861 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     4921 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
     4981 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     5041 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     5101 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     5161 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     5221 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     5281 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     5341 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     5401 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     5461 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     5521 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     5581 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     5641 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     5701 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     5761 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     5821 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     5881 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5941 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     6001 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6061 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6121 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6181 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6241 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6301 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6361 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6421 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6481 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6541 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6601 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6661 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6721 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6781 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6841 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     6901 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     6961 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7021 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7081 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7141 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7201 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7261 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7321 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7381 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7441 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7501 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7561 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7621 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7681 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7741 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7801 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     7861 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     7921 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     7981 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     8041 gaaaagtgcc acctgacgtc
//