| 质粒名称: | pAAVLINK-3'-ABCA7-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001710 |
| 产品用途: | 3' AAVLINK plasmid for ABCA7 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ABCA7 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-ABCA7-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-ABCA7-FLAG载体质粒基本信息
pAAVLINK-3'-ABCA7-FLAG质粒图谱载体图谱和pAAVLINK-3'-ABCA7-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-ABCA7-FLAG质粒载体简介
pAAVLINK-3'-ABCA7-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-ABCA7-FLAG 7242 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7242)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7242
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3981
/codon_start=1
/label=ABCA7
/translation="GLSAAVVGPEQDRLLQDVGLVSKQSVQTRHLSGGMQRKLSVAIAF
VGGSQVVILDEPTAGVDPASRRGIWELLLKYREGRTLILSTHHLDEAELLGDRVAVVAG
GRLCCCGSPLFLRRHLGSGYYLTLVKARLPLTTNEKADTDMEGSVDTRQEKKNGSQGSR
VGTPQLLALVQHWVPGARLVEELPHELVLVLPYTGAHDGSFATLFRELDTRLAELRLTG
YGISDTSLEEIFLKVVEECAADTDMEDGSCGQHLCTGIAGLDVTLRLKMPPQETALENG
EPAGSAPETDQGSGPDAVGRVQGWALTRQQLQALLLKRFLLARRSRRGLFAQIVLPALF
VGLALVFSLIVPPFGHYPALRLSPTMYGAQVSFFSEDAPGDPGRARLLEALLQEAGLEE
PPVQHSSHRFSAPEVPAEVAKVLASGNWTPESPSPACQCSRPGARRLLPDCPAAAGGPP
PPQAVTGSGEVVQNLTGRNLSDFLVKTYPRLVRQGLKTKKWVNEVRYGGFSLGGRDPGL
PSGQELGRSVEELWALLSPLPGGALDRVLKNLTAWAHSLDAQDSLKIWFNNKGWHSMVA
FVNRASNAILRAHLPPGPARHAHSITTLNHPLNLTKEQLSEGALMASSVDVLVSICVVF
AMSFVPASFTLVLIEERVTRAKHLQLMGGLSPTLYWLGNFLWDMCNYLVPACIVVLIFL
AFQQRAYVAPANLPALLLLLLLYGWSITPLMYPASFFFSVPSTAYVVLTCINLFIGING
SMATFVLELFSDQKLQEVSRILKQVFLIFPHFCLGRGLIDMVRNQAMADAFERLGDRQF
QSPLRWEVVGKNLLAMVIQGPLFLLFTLLLQHRSQLLPQPRVRSLPLLGEEDEDVARER
ERVVQGATQGDVLVLRNLTKVYRGQRMPAVDRLCLGIPPGECFGLLGVNGAGKTSTFRM
VTGDTLASRGEAVLAGHSVAREPSAAHLSMGYCPQSDAIFELLTGREHLELLARLRGVP
EAQVAQTAGSGLARLGLSWYADRPAGTYSGGNKRKLATALALVGDPAVVFLDEPTTGMD
PSARRFLWNSLLAVVREGRSVMLTSHSMEECEALCSRLAIMVNGRFRCLGSPQHLKGRF
AAGHTLTLRVPAARSQPAAAFVAAEFPGAELREAHGGRLRFQLPPGGRCALARVFGELA
VHGAEHGVEDFSVSQTMLEEVFLYFSKDQGKDEDTEEQKEAGVGVDPAPGLQHPKRVSQ
FLDDPSTAETVL"
misc_feature 1324..1329
/label=SDS
misc_feature 1448..1453
/label=SDS
regulatory 1988..1997
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 2135..2140
/label=SDS
misc_feature 2453..2458
/label=SDS
misc_feature 2576..2581
/label=SDS
misc_feature 2855..2860
/label=SDS
misc_feature 3824..3829
/label=SDS
misc_feature 3884..3889
/label=SDS
CDS 3988..4053
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 4057..4488
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4201..4206
/label=SDS
repeat_region 4505..4645
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4505..4634
/label=AAV2 ITR
rep_origin 4720..5175
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5457..5561
/gene="bla"
/label=AmpR promoter
CDS 5562..6422
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6593..7181
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag ggtctgagtg ccgctgtagt gggccccgag
301 caggaccgtc tgctgcagga tgtggggctg gtctccaagc agagtgtgca gactcgccac
361 ctctctggtg ggatgcaacg gaagctgtcc gtggccattg cctttgtggg cggctcccaa
421 gttgttatcc tggacgagcc tacggctggc gtggatcctg cttcccgccg cggtatttgg
481 gagctgctgc tcaaataccg agaaggtcgc acgctgatcc tctccaccca ccacctggat
541 gaggcagagc tgctgggaga ccgtgtggcc gtggtggcag gtggccgctt gtgctgctgt
601 ggctccccac tcttcctgcg ccgtcacctg ggctccggct actacctgac gctggtgaag
661 gcccgcctgc ccctgaccac caatgagaag gctgacactg acatggaggg cagtgtggac
721 accaggcagg aaaagaagaa tggcagccag ggcagcagag tcggcactcc tcagctgctg
781 gccctggtac agcactgggt gcccggggca cggctggtgg aggagctgcc acacgagctg
841 gtgctggtgc tgccctacac gggtgcccat gacggcagct tcgccacact cttccgagag
901 ctagacacgc ggctggcgga gctgaggctc actggctacg ggatctccga caccagcctc
961 gaggagatct tcctgaaggt ggtggaggag tgtgctgcgg acacagatat ggaggatggc
1021 agctgcgggc agcacctatg cacaggcatt gctggcctag acgtaaccct acggctcaag
1081 atgccgccac aggagacagc gctggagaac ggggaaccag ctgggtcagc cccagagact
1141 gaccagggct ctgggccaga cgccgtgggc cgggtacagg gctgggcact gacccgccag
1201 cagctccagg ccctgcttct caagcgcttt ctgcttgccc gccgcagccg ccgcggcctg
1261 ttcgcccaga tcgtgctgcc tgccctcttt gtgggcctgg ccctcgtgtt cagcctcatc
1321 gtgcctcctt tcgggcacta cccggctctg cggctcagtc ccaccatgta cggtgctcag
1381 gtgtccttct tcagtgagga cgccccaggg gaccctggac gtgcccggct gctcgaggcg
1441 ctgctgcagg aggcaggact ggaggagccc ccagtgcagc atagctccca caggttctcg
1501 gcaccagaag ttcctgctga agtggccaag gtcttggcca gtggcaactg gaccccagag
1561 tctccatccc cagcctgcca gtgtagccgg cccggtgccc ggcgcctgct gcccgactgc
1621 ccggctgcag ctggtggtcc ccctccgccc caggcagtga ccggctctgg ggaagtggtt
1681 cagaacctga caggccggaa cctgtctgac ttcctggtca agacctaccc gcgcctggtg
1741 cgccagggcc tgaagactaa gaagtgggtg aatgaggtca gatacggagg cttctcgctg
1801 gggggccgag acccaggcct gccctcgggc caagagttgg gccgctcagt ggaggagttg
1861 tgggcgctgc tgagtcccct gcctggcggg gccctcgacc gtgtcctgaa aaacctcaca
1921 gcctgggctc acagcctgga tgctcaggac agtctcaaga tctggttcaa caacaaaggc
1981 tggcactcca tggtggcctt tgtcaaccga gccagcaacg caatcctccg tgctcacctg
2041 cccccaggcc cggcccgcca cgcccacagc atcaccacac tcaaccaccc cttgaacctc
2101 accaaggagc agctgtctga gggtgcactg atggcctcct cggtggacgt cctcgtctcc
2161 atctgtgtgg tctttgccat gtcctttgtc ccggccagct tcactcttgt cctcattgag
2221 gagcgagtca cccgagccaa gcacctgcag ctcatggggg gcctgtcccc caccctctac
2281 tggcttggca actttctctg ggacatgtgt aactacttgg tgccagcatg catcgtggtg
2341 ctcatctttc tggccttcca gcagagggca tatgtggccc ctgccaacct gcctgctctc
2401 ctgctgttgc tactactgta tggctggtcg atcacaccgc tcatgtaccc agcctccttc
2461 ttcttctccg tgcccagcac agcctatgtg gtgctcacct gcataaacct ctttattggc
2521 atcaatggaa gcatggccac ctttgtgctt gagctcttct ctgatcagaa gctgcaggag
2581 gtgagccgga tcttgaaaca ggtcttcctt atcttccccc acttctgctt gggccggggg
2641 ctcattgaca tggtgcggaa ccaggccatg gctgatgcct ttgagcgctt gggagacagg
2701 cagttccagt cacccctgcg ctgggaggtg gtcggcaaga acctcttggc catggtgata
2761 caggggcccc tcttccttct cttcacacta ctgctgcagc accgaagcca actcctgcca
2821 cagcccaggg tgaggtctct gccactcctg ggagaggagg acgaggatgt agcccgtgaa
2881 cgggagcggg tggtccaagg agccacccag ggggatgtgt tggtgctgag gaacttgacc
2941 aaggtatacc gtgggcagag gatgccagct gttgaccgct tgtgcctggg gattccccct
3001 ggtgagtgtt ttgggctgct gggtgtgaat ggagcaggga agacgtccac gtttcgcatg
3061 gtgacggggg acacattggc cagcaggggc gaggctgtgc tggcaggcca cagcgtggcc
3121 cgggaaccca gtgctgcgca cctcagcatg ggatactgcc ctcaatccga tgccatcttt
3181 gagctgctga cgggccgcga gcacctggag ctgcttgcgc gcctgcgcgg tgtcccggag
3241 gcccaggttg cccagaccgc tggctcgggc ctggcgcgtc tgggactctc atggtacgca
3301 gaccggcctg caggcaccta cagcggaggg aacaaacgca agctggcgac ggccctggcg
3361 ctggttgggg acccagccgt ggtgtttctg gacgagccga ccacaggcat ggaccccagc
3421 gcgcggcgct tcctttggaa cagccttttg gccgtggtgc gggagggccg ttcagtgatg
3481 ctcacctccc atagcatgga ggagtgtgaa gcgctctgct cgcgcctggc catcatggtg
3541 aatgggcggt tccgctgcct gggcagcccg caacatctca agggcagatt cgcggcgggt
3601 cacacactga ccctgcgggt gcccgccgca aggtcccagc cggcagcggc cttcgtggcg
3661 gccgagttcc ctggggcgga gctgcgcgag gcacatggag gccgcctgcg cttccagctg
3721 ccgccgggag ggcgctgcgc cctggcgcgc gtctttggag agctggcggt gcacggcgca
3781 gagcacggcg tggaggactt ttccgtgagc cagacgatgc tggaggaggt attcttgtac
3841 ttctccaagg accaggggaa ggacgaggac accgaagagc agaaggaggc aggagtggga
3901 gtggaccccg cgccaggcct gcagcacccc aaacgcgtca gccagttcct cgatgaccct
3961 agcactgccg agactgtgct ctgtacagac tacaaagacc atgacggtga ttataaagat
4021 catgacatcg actacaagga tgacgatgac aagtaactcg agataatcaa cctctggatt
4081 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
4141 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
4201 cctccttgta taaatcctgg ttagttcttg ccacggcgga actcatcgcc gcctgccttg
4261 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg tttatttgtg
4321 aaatttgtga tgctattgct ttatttgtaa ccatctagct ttatttgtga aatttgtgat
4381 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
4441 attcatttta tgtttcaggt tcagggggag atgtgggagg ttttttaacg gaccgagcgg
4501 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
4561 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
4621 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
4681 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
4741 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
4801 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
4861 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
4921 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
4981 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
5041 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
5101 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
5161 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
5221 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
5281 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
5341 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
5401 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
5461 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
5521 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
5581 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
5641 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
5701 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5761 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
5821 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5881 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5941 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
6001 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
6061 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
6121 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
6181 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
6241 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
6301 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
6361 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
6421 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
6481 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
6541 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
6601 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6661 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6721 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
6781 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6841 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6901 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6961 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
7021 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
7081 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
7141 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
7201 ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
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pAAVLINK-3'-ABCA7-FLAG质粒载体应用举例
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