| 质粒名称: | pGEX-6p-1-NUFIP2 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000946 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-6p-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | NUFIP2 |
| 物种来源: | human |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-6p-1-NUFIP2载体质粒基本信息
pGEX-6p-1-NUFIP2质粒图谱载体图谱和pGEX-6p-1-NUFIP2载体序列质粒序列多克隆位点信息
pGEX-6p-1-NUFIP2质粒载体简介
pGEX-6p-1-NUFIP2质粒序列载体序列
LOCUS pGEX-6p-1-NUFIP2 7078 bp DNA circular SYN 03-NOV-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7078)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 17, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7078
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
primer_bind complement(939..977)
/label=YZY-P793
CDS 951..971
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
misc_feature 957..995
/label=Primer F for NUFIP2 in pGEX-6P-1
CDS 978..3062
/codon_start=1
/label=NUFIP2 Codon
/translation="MEEKPGQPQPQHHHSHHHPHHHPQQQQQQPHHHHHYYFYNHSHNH
HHHHHHQQPHQYLQHGAEGSPKAQPKPLKHEQKHTLQQHQETPKKKTGYGELNGNAGER
EISLKNLSSDEATNPISRVLNGNQQVVDTSLKQTVKANTFGKAGIKTKNFIQKNSMDKK
NGKSYENKSGENQSVDKSDTIPIPNGVVTNNSGYITNGYMGKGADNDGSGSESGYTTPK
KRKARRNSAKGCENLNIVQDKIMQQETSVPTLKQGLETFKPDYSEQKGNRVDGSKPIWK
YETGPGGTSRGKPAVGDMLRKSSDSKPGVSSKKFDDRPKGKHASAVASKEDSWTLFKPP
PVFPVDNSSAKIVPKISYASKVKENLNKTIQNSSVSPTSSSSSSSSTGETQTQSSSRLS
QVPMSALKSVTSANFSNGPVLAGTDGNVYPPGGQPLLTTAANTLTPISSGTDSVLQDMS
LTSAAVEQIKTSLFIYPSNMQTMLLSTAQVDLPSQTDQQNLGDIFQNQWGLSFINEPSA
GPETVTGKSSEHKVMEVTFQGEYPATLVSQGAEIIPSGTEHPVFPKAYELEKRTSPQVL
GSILKSGTTSESGALSLEPSHIGDLQKADTSSQGALVFLSKDYEIESQNPLASPTNTLL
GSAKEQRYQRGLERNDSWGSFDLRAAIVYHTKEMESIWNLQKQDPKRIITYNEAMDSPD
Q"
CDS 1110..1130
/codon_start=1
/product="6xHis affinity tag"
/label=7xHis
/translation="HHHHHHH"
misc_feature complement(3045..3083)
/label=Primer R for NUFIP2 in Pgex-6p-1
primer_bind 3063..3101
/label=YZY-P792
promoter 3381..3485
/gene="bla"
/label=AmpR promoter
CDS 3486..4346
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4517..5105
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 5349..5426
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 5427..6509
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 6522..6543
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6558..6588
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6596..6612
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6620..6636
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 6632..7078
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(6652..6668)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc gaaaacctgt
961 atttccaggg cggtagtatg gaagaaaaac cgggccagcc gcagccgcag catcatcata
1021 gtcatcatca tccgcatcat catcctcagc agcagcagca acagccgcat catcaccatc
1081 attattattt ttataaccac agccacaacc atcatcatca tcaccaccat cagcagccgc
1141 atcagtatct gcagcatggt gcagaaggca gcccgaaagc ccagccgaaa ccgctgaaac
1201 atgaacagaa acataccctg cagcagcatc aggaaacccc gaaaaagaaa accggctatg
1261 gtgaactgaa tggtaatgcc ggtgaacgtg aaattagcct gaaaaatctg agtagcgatg
1321 aagccaccaa tccgattagt cgcgttctga atggtaacca gcaggtggtt gataccagtc
1381 tgaaacagac cgtgaaagca aatacctttg gtaaagcagg tattaagacc aaaaatttta
1441 tccagaagaa cagcatggat aaaaagaatg gtaaaagtta cgagaacaag agtggtgaaa
1501 atcagagcgt ggataaaagt gataccattc cgattccgaa tggtgttgtt accaataata
1561 gcggctatat taccaatggc tatatgggca aaggtgccga taatgatggt agcggtagcg
1621 aaagcggcta taccaccccg aaaaaacgca aagcccgtcg taatagtgca aaaggttgcg
1681 aaaatctgaa tattgtgcag gataaaatca tgcagcagga aaccagcgtt ccgaccctga
1741 aacagggcct ggaaaccttt aaaccggatt atagcgaaca gaaaggcaat cgcgttgatg
1801 gcagcaaacc gatttggaaa tatgaaaccg gtccgggcgg caccagtcgt ggcaaaccgg
1861 ctgttggtga catgctgcgc aaaagtagtg atagcaaacc gggtgttagt agcaaaaaat
1921 ttgatgatcg cccgaaaggc aaacatgcaa gtgccgtggc cagcaaagaa gatagttgga
1981 ccctgtttaa accgccgccg gtttttccgg tggataatag cagcgcaaaa attgtgccga
2041 aaattagcta tgcaagcaaa gtgaaagaaa atctgaacaa aaccatccag aatagcagtg
2101 ttagtccgac cagtagcagt agtagtagta gcagtaccgg tgaaacccag acccagagta
2161 gcagtcgtct gagccaggtg ccgatgagcg cactgaaaag tgtgaccagt gcaaatttta
2221 gcaatggtcc ggtgctggca ggcaccgatg gcaatgttta tccgccgggt ggccagccgc
2281 tgctgaccac cgcagcaaat accctgaccc cgattagcag cggcaccgat agcgtgctgc
2341 aggatatgag tctgaccagc gccgccgtgg aacagattaa gaccagcctg tttatctatc
2401 cgagcaatat gcagaccatg ctgctgagca ccgcacaggt tgatctgccg agtcagaccg
2461 atcagcagaa tctgggtgac atttttcaga atcagtgggg tctgagtttt attaatgaac
2521 cgagcgcagg cccggaaacc gtgaccggta aaagtagcga acataaagtg atggaagtta
2581 cctttcaggg cgaatatccg gcaaccctgg ttagccaggg cgcagaaatt attccgagtg
2641 gcaccgaaca tccggtgttt ccgaaagcat acgaactgga aaaacgcacc agtccgcagg
2701 tgctgggtag cattctgaaa agcggtacaa ccagtgaaag cggcgccctg agcctggaac
2761 cgagtcatat tggtgacctg cagaaagccg ataccagcag tcagggtgca ttagtgtttc
2821 tgagcaaaga ttatgaaatc gaaagtcaga atccgctggc cagcccgacc aataccctgc
2881 tgggcagtgc aaaagaacag cgttatcagc gcggcctgga acgtaatgat agttggggta
2941 gttttgatct gcgtgcagca attgtgtatc ataccaaaga aatggaaagc atttggaatc
3001 tgcagaaaca ggaccctaaa cgtattatta cctataatga agcaatggat agcccggatc
3061 agctcgagcg gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg
3121 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
3181 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
3241 ccatgaccca gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg
3301 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
3361 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
3421 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
3481 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
3541 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
3601 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
3661 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
3721 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
3781 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
3841 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
3901 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
3961 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
4021 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
4081 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
4141 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
4201 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
4261 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
4321 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
4381 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
4441 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
4501 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
4561 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
4621 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
4681 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
4741 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
4801 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
4861 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
4921 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
4981 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
5041 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
5101 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
5161 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
5221 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
5281 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
5341 taaattccga caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg
5401 aagagagtca attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt
5461 atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg
5521 cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg
5581 tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg
5641 ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg
5701 ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc
5761 acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg
5821 atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg
5881 accagacacc catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg
5941 agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg
6001 tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc
6061 cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa
6121 tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg
6181 gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg
6241 gatacgacga taccgaagac agctcatgtt atatcccgcc gtcaaccacc atcaaacagg
6301 attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg
6361 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc
6421 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
6481 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
6541 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
6601 agcggataac aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt
6661 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
6721 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
6781 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt
6841 gccggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa
6901 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt
6961 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt
7021 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt
//
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