| 质粒名称: | pCDH_hsaMTS-GFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002302 |
| 产品用途: | Expression of MTS-GFP in U2OS cells |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
|
插入基因信息 |
|
| 插入基因名称: | hsaOTC-GFP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein
Runqing Yang, Yingying Wang, Minghua Kong, Zhijuan Hu,Zhe Zhang, Kun Shen, Jiali Meng & An-Ping Zeng
Nature Communications,16,20250807
|
pCDH_hsaMTS-GFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCDH_hsaMTS-GFP载体质粒基本信息
pCDH_hsaMTS-GFP质粒图谱载体图谱和pCDH_hsaMTS-GFP载体序列质粒序列多克隆位点信息
pCDH_hsaMTS-GFP质粒载体简介
pCDH_hsaMTS-GFP质粒序列载体序列
LOCUS pCDH_hsaMTS-GFP 7272 bp DNA circular SYN 03-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7272)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7272
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 6..232
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 233..413
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 460..585
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1078..1311
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1496..1540
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 1807..1923
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 1946..2249
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2250..2453
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2507..2599
/label=hsaOTC
CDS 2600..3313
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 3353..3940
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(3824..3835)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 4013..4246
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 4318..4439
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 4479..4614
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(4647..4663)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4671..4687
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4695..4725)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4740..4761
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5049..5637)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5808..6668)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6669..6773)
/gene="bla"
/label=AmpR promoter
primer_bind 7247..7263
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
1921 tttatcgata ctagttggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
1981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
2041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
2101 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
2161 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
2221 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
2281 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
2341 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
2401 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
2461 tgaaccgtca gattctagac cgctagcgct accggtcgcc accatgctgt ttaatctgag
2521 gatcctgtta aacaatgcag cttttagaaa tggtcacaac ttcatggttc gaaattttcg
2581 gtgtggacaa ccactacaag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
2641 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
2701 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
2761 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
2821 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
2881 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
2941 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
3001 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
3061 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
3121 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
3181 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
3241 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
3301 cgagctgtac aagtccggac tcagatctcg agctcaagct tcgtagggat ccaatcaacc
3361 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
3421 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
3481 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
3541 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
3601 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
3661 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
3721 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
3781 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
3841 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc tccgccttcg
3901 ccctcagacg agtcggatct ccctttggcc gcctccccgc cggtaccttt aagaccaatg
3961 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
4021 ctaattcact cccaacgaag ataagatctg ctttttgctt gtactgggtc tctctggtta
4081 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
4141 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
4201 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
4261 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac
4321 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
4381 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
4441 catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca
4501 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
4561 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact
4621 tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
4681 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
4741 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
4801 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
4861 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
4921 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
4981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accttaaaaa ggccgcgttg
5041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
5101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
5161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
5221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
5281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
5341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
5401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
5461 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
5521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
5581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
5641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
5701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
5761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
5821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
5881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
5941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
6001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
6061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
6121 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
6181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
6241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
6301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
6361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
6421 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
6481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
6541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
6601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
6661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
6721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
6781 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
6841 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
6901 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
6961 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
7021 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
7081 gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa
7141 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
7201 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
7261 agtgccaagc tg
//
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