| 质粒名称: | pCAG-VSVG |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 64084 |
| 质粒用途: | 水泡性口炎病毒印第安纳株糖蛋白基因的表达载体 |
| 存储实验室: | Ian Wickersham |
| 相关质粒: |
pCAG-VSVG pCAG-VSVL pCAG-VSVM pCAG-VSVN pCAG-VSVP |
|
载体骨架基本信息 |
|
| 载体名称: | pCAGEN |
| 空载体大小: | 4795 bp |
| 完整质粒大小: | 6331 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | VSV glycoprotein |
| 物种来源: | Vesicular Stomatitis Virus |
| 插入大小: | 1536 bp |
| 启动子: | CAG |
| 克隆方法: | 未知 |
| 5’测序引物 | CTCTAGAGCCTCTGCTAACc |
| 3’测序引物 | CAGAAGTCAGATGCTCAAG |
|
文献引用方法 |
|
| 材料和方法部分: | pCAG-VSVG was a gift from Ian Wickersham (Addgene plasmid # 64084 ; http://n2t.net/addgene:64084 ; RRID:Addgene_64084) |
| 参考文献部分: | 未发表 |
买家导航
pCAG-VSVG载体质粒基本信息
pCAG-VSVG质粒图谱载体图谱和pCAG-VSVG载体序列质粒序列多克隆位点信息
pCAG-VSVG质粒载体简介
pCAG-VSVG质粒序列载体序列
LOCUS pCAG-VSVG 6331 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expression vector for Vesicular Stomatitis Virus Indiana strain
glycoprotein gene.
ACCESSION .
VERSION .
KEYWORDS pCAG-VSVG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6331)
TITLE Genetic Neuroengineering Group Plasmids
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6331)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6331
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 4..383
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 386..661
/label=chicken beta-actin promoter
intron 662..1670
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 1594..1616
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 1678..1697
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
CDS 1734..3269
/codon_start=1
/product="vesicular stomatitis virus G glycoprotein"
/label=VSV-G
/note="Indiana strain"
/translation="MKCLLYLTFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD
LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS
VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ
FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR
SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT
SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK
YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP
LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF
SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK"
primer_bind complement(3355..3374)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_signal 3420..3475
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
primer_bind complement(3474..3493)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3834..3850)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3834..3850)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3847..3869)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3858..3874
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3882..3912)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3927..3948
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4007..4202
/label=SV40 promoter
/note="SV40 early promoter"
rep_origin 4053..4188
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4115..4134
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
polyA_signal 4208..4342
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4245..4264)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 4299..4318
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(4410..4427)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4581..5169)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4661..4680)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5340..6200)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5963..5982
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6201..6305)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccgcc accatgaagt
1741 gccttttgta cttaaccttt ttattcattg gggtgaattg caagttcacc atagtttttc
1801 cacacaacca aaaaggaaac tggaaaaatg ttccttctaa ttaccattat tgcccgtcaa
1861 gctcagattt aaattggcat aatgacttaa taggcacagc cttacaagtc aaaatgccca
1921 agagtcacaa ggctattcaa gcagacggtt ggatgtgtca tgcttccaaa tgggtcacta
1981 cttgtgattt ccgctggtat ggaccgaagt atataacaca ttccatccga tccttcactc
2041 catctgtaga acaatgcaag gaaagcattg aacaaacgaa acaaggaact tggctgaatc
2101 caggcttccc tcctcaaagt tgtggatatg caactgtgac ggatgccgaa gcagtgattg
2161 tccaggtgac tcctcaccat gtgctggttg atgaatacac aggagaatgg gttgattcac
2221 agttcatcaa cggaaaatgc agcaattaca tatgccccac tgtccataac tctacaacct
2281 ggcattctga ctataaggtc aaagggctat gtgattctaa cctcatttcc atggacatca
2341 ccttcttctc agaggacgga gagctatcat ccctgggaaa ggagggcaca gggttcagaa
2401 gtaactactt tgcttatgaa actggaggca aggcctgcaa aatgcaatac tgcaagcatt
2461 ggggagtcag actcccatca ggtgtctggt tcgagatggc tgataaggat ctctttgctg
2521 cagccagatt ccctgaatgc ccagaagggt caagtatctc tgctccatct cagacctcag
2581 tggatgtaag tctaattcag gacgttgaga ggatcttgga ttattccctc tgccaagaaa
2641 cctggagcaa aatcagagcg ggtcttccaa tctctccagt ggatctcagc tatcttgctc
2701 ctaaaaaccc aggaaccggt cctgctttca ccataatcaa tggtacccta aaatactttg
2761 agaccagata catcagagtc gatattgctg ctccaatcct ctcaagaatg gtcggaatga
2821 tcagtggaac taccacagaa agggaactgt gggatgactg ggcaccatat gaagacgtgg
2881 aaattggacc caatggagtt ctgaggacca gttcaggata taagtttcct ttatacatga
2941 ttggacatgg tatgttggac tccgatcttc atcttagctc aaaggctcag gtgttcgaac
3001 atcctcacat tcaagacgct gcttcgcaac ttcctgatga tgagagttta ttttttggtg
3061 atactgggct atccaaaaat ccaatcgagc ttgtagaagg ttggttcagt agttggaaaa
3121 gctctattgc ctcttttttc tttatcatag ggttaatcat tggactattc ttggttctcc
3181 gagttggtat ccatctttgc attaaattaa agcacaccaa gaaaagacag atttatacag
3241 acatagagat gaaccgactt ggaaagtaag cggccgcact cctcaggtgc aggctgccta
3301 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt
3361 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta
3421 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg
3481 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt
3541 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt
3601 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
3661 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
3721 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
3781 ctcttctctt atgaagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag
3841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
3901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
3961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt
4021 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
4081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
4141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
4201 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
4261 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
4321 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc
4381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
4441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
4501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
4561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
4621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
4681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
4741 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
4801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
4861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
4921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
4981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
5041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
5101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
5161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
5221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
5281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
5341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
5401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
5461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
5521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
5581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
5641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
5701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
5761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
5821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
5881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
5941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
6001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
6061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
6121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
6181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
6241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
6301 ccgcgcacat ttccccgaaa agtgccacct g
//
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