pQL84

LOCUS       pQL84            7543 bp DNA     circular SYN 16-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 29, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=HIV-1 5 LTR
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1126..1170
                     /label=HIV-1 psi pack
     misc_feature    1222..1227
                     /label=U6 terminator
     misc_feature    1251..1256
                     /label=U6 terminator
     misc_feature    1665..1670
                     /label=U6 terminator
     misc_feature    1684..1917
                     /label=RRE
     misc_feature    2444..2561
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2620..2932
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     misc_feature    2845..2850
                     /label=U6 terminator
     primer_bind     2949..2965
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2994..3008)
                     /label=QL152-R
     misc_recomb     3007..3040
                     /label=LoxP
     protein_bind    3007..3040
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     primer_bind     3048..3088
                     /label=QL143-F
     misc_feature    3089..3116
                     /label=GGAA
     misc_feature    3117..3144
                     /label=GGAA
     misc_feature    3145..3172
                     /label=GGAA
     misc_feature    3179..3326
                     /label=hcmvmin
     misc_feature    3248..3262
                     /label=New Feature
     primer_bind     complement(3311..3326)
                     /label=oYW11
     primer_bind     3318..3357
                     /label=QL111 E1A-F
     primer_bind     3337..3357
                     /label=E1-F
     CDS             join(3338..3890,4007..4323)
                     /codon_start=1
                     /product="E1A"
                     /label=E1A
                     /note="other spliced forms exist with larger introns"
                     /protein_id="DAA00639.1"
                     /translation="MRHIICHGGVITEEMAASLLDQLIEEVLADNLPPPSHFEPPTLHE
                     LYDLDVTAPEDPNEEAVSQIFPDSVMLAVQEGIDLLTFPPAPGSPEPPHLSRQPEQPEQ
                     RALGPVSMPNLVPEVIDLTCHEAGFPPSDDEDEEGEEFVLDYVEHPGHGCRSCHYHRRN
                     TGDPDIMCSLCYMRTCGMFVYSPVSEPEPEPEPEPEPARPTRRPKMAPAILRRPTSPVS
                     RECNSSTDSCDSGPSNTPPEIHPVVPLCPIKPVAVRVGGRRQAVECIEDLLNEPGQPLD
                     LSCKRPRP"
     primer_bind     3338..3357
                     /label=QL43
     primer_bind     complement(4303..4344)
                     /label=QL152-R
     primer_bind     complement(4304..4324)
                     /label=QL44
     primer_bind     complement(4304..4324)
                     /label=QL112 E1A-R
     primer_bind     complement(4305..4324)
                     /label=E1-R
     misc_recomb     4343..4376
                     /label=LoxP
     protein_bind    4343..4376
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    4432..5020
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4903..4914)
                     /label=FactorXa site
     primer_bind     complement(5023..5039)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             5549..5729
                     /label=HIV-1 5 LTR
     rep_origin      complement(5773..6401)
                     /direction=LEFT
                     /label=ColE1 origin
     rep_origin      complement(5791..6379)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6331..6336
                     /label=U6 terminator
     misc_feature    6332..6337
                     /label=U6 terminator
     misc_feature    6364..6369
                     /label=U6 terminator
     misc_feature    6365..6370
                     /label=U6 terminator
     CDS             complement(6550..7410)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             complement(6553..7212)
                     /label=AmpR
     misc_feature    6962..6967
                     /label=U6 terminator
     misc_feature    7369..7374
                     /label=U6 terminator
     promoter        complement(7411..7515)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        complement(7452..7480)
                     /label=Amp prom
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
     2641 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
     2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
     2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataagag
     2821 atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta
     2881 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg
     2941 gtgaccctcg aggtcgacgg tatcgataag ctcgcttcac gagattccag caggtcgagg
     3001 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccaagcttaa
     3061 gcggccgcgg ccagatatac gcgtgtacgg aaggaaggaa ggaaggaagg aaggaaggaa
     3121 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aagctagctc
     3181 gagctcggta cccgggtcga gtaggcgtgt acggtgggag gcctatataa gcagagctcg
     3241 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
     3301 acaccgggac cgatccagcc tccgcggccc cgaattcatg agacatatta tctgccacgg
     3361 aggtgttatt accgaagaaa tggccgccag tcttttggac cagctgatcg aagaggtact
     3421 ggctgataat cttccacctc ctagccattt tgaaccacct acccttcacg aactgtatga
     3481 tttagacgtg acggcccccg aagatcccaa cgaggaggcg gtttcgcaga tttttcccga
     3541 ctctgtaatg ttggcggtgc aggaagggat tgacttactc acttttccgc cggcgcccgg
     3601 ttctccggag ccgcctcacc tttcccggca gcccgagcag ccggagcaga gagccttggg
     3661 tccggtttct atgccaaacc ttgtaccgga ggtgatcgat cttacctgcc acgaggctgg
     3721 ctttccaccc agtgacgacg aggatgaaga gggtgaggag tttgtgttag attatgtgga
     3781 gcaccccggg cacggttgca ggtcttgtca ttatcaccgg aggaatacgg gggacccaga
     3841 tattatgtgt tcgctttgct atatgaggac ctgtggcatg tttgtctaca gtaagtgaaa
     3901 attatgggca gtgggtgata gagtggtggg tttggtgtgg taattttttt tttaattttt
     3961 acagttttgt ggtttaaaga attttgtatt gtgatttttt taaaaggtcc tgtgtctgaa
     4021 cctgagcctg agcccgagcc agaaccggag cctgcaagac ctacccgccg tcctaaaatg
     4081 gcgcctgcta tcctgagacg cccgacatca cctgtgtcta gagaatgcaa tagtagtacg
     4141 gatagctgtg actccggtcc ttctaacaca cctcctgaga tacacccggt ggtcccgctg
     4201 tgccccatta aaccagttgc cgtgagagtt ggtgggcgtc gccaggctgt ggaatgtatc
     4261 gaggacttgc ttaacgagcc tgggcaacct ttggacttga gctgtaaacg ccccaggcca
     4321 taagaattcg tcgagggacc taataacttc gtatagcata cattatacga agttatacat
     4381 gtttaagggt tccggttcca ctaggtacaa ttcgatatca agcttatcga taatcaacct
     4441 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     4501 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     4561 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     4621 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     4681 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     4741 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     4801 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     4861 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     4921 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     4981 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     5041 tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga
     5101 ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcaggt
     5161 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5221 ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg atctgtggat
     5281 ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc cagggatcag
     5341 atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag agaaggtaga
     5401 agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc atgggatgga
     5461 tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat ttcatcacat
     5521 ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     5581 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     5641 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     5701 cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc aaaaggccag
     5761 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5821 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5881 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5941 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6001 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6061 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6121 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6181 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6241 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6301 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6361 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     6421 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     6481 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6541 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6601 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6661 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6721 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6781 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6841 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6901 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6961 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7021 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7081 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7141 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7201 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7261 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7321 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7381 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7441 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7501 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//