pBsVchCAST-pyrE

LOCUS       pBsVchCAST-pyrE               16303 bp ds-DNA     circular SYN 11-AUG-2023
DEFINITION  Expression of CRISPR-associated transposases, shuttle vector for 
            Bacillus subtilis / E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    pBsVchCAST-pyrE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16303)
  TITLE     Sheng Yang Plasmids 2023
REFERENCE   2  (bases 1 to 16303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 11, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..16303
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     1221..1243
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1235..1252
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1236..1252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1295..2377)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     2339..2358
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    2761..2785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             9806..9817
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        14380..14484
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14485..15345
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14703..14722)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15516..16104
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16005..16024
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16258..16275
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
       61 tgtatcgtta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata ttataaaagc
      121 cagtcattag gcctatctga caattcctga atagagttca taaacaatcc tgcatgataa
      181 ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga attaccttta
      241 ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt attgatattt
      301 aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc attttcaggt
      361 ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc agaaaggtat
      421 aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga gaatgtttta
      481 gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc taactctccg
      541 tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt cactaagaaa
      601 ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa aacactaata
      661 tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt aaatatctct
      721 tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc ctcctaaatt
      781 tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga atcaatcctt
      841 ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc cactttatcc
      901 aattttcgtt tgttgaacta atgggtgctt tagttgaaga ataaaagacc acattaaaaa
      961 atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt
     1021 atcttgataa taagggtaac tattgccgtc gtccattccg acagcatcgc cagtcactat
     1081 ggcgtgctgc tagcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     1141 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     1201 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
     1261 gctcaggcct taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1321 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1381 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     1441 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     1501 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     1561 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     1621 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     1681 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     1741 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     1801 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     1861 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     1921 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     1981 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2041 tgtgcaccgc cgttttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2101 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     2161 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     2221 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     2281 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     2341 catactctgc gacatcgtat aacgttactg gtttcatcaa aatcgtctcc ctccgtttga
     2401 atatttgatt gatcgtaacc agatgaagca ctctttccac tatccctaca gtgttatggc
     2461 ttgaacaatc acgaaacaat aattggtacg tacgatcttt cagccgactc aaacatcaaa
     2521 tcttacaaat gtagtctttg aaagtattac atatgtaaga tttaaatgca accgtttttt
     2581 cggaaggaaa tgatgacctc gtttccaccg gaattagctt ggtaccagct attgtaacat
     2641 aatcggtacg ggggtgaaaa agctaacgga aaagggagcg gaaaagaatg atgtaagcgt
     2701 gaaaaatttt ttatcttatc acttgaaatt ggaagggaga ttctttatta taagaattgt
     2761 ggaattgtga gcggataaca attcccaatt aaaggaggaa ggatcatagg atccgtgaac
     2821 tgccgagtag gtagctgata acacaggagg cagcgtgctt gaagcttgtg cagcgtgaac
     2881 tgccgagtag gtagctgata acgaattcga gctcggcgcg cctgcaggtc gacaagcttg
     2941 cggccgcaag gagatataca tatgtttttg caaagaccta aaccttacag cgatgaaagt
     3001 ttagaaagtt tctttatccg agtggctaac aaaaatggct acggtgatgt ccatcgcttc
     3061 ctagaagcca ctaaacgatt ccttcaagac attgaccata atggctatca aacctttccg
     3121 actgatataa ctcggataaa cccatactca gctaaaaaca gttccagcgc acgaactgcg
     3181 tcattcctga aacttgcaca attgacattt aatgaaccgc cagagctact tgggttggca
     3241 attaacagaa caaacatgaa atactcgccg tcaactagcg cggttgttag aggtgcagaa
     3301 gtctttcctc gcagtttact acggacgcac tccatcccct gctgtccttt gtgtctgcga
     3361 gaaaatggct acgcctccta cctttggcac tttcaggggt acgaatactg ccacagccat
     3421 aacgtacctt taattaccac ttgtagctgt ggtaaggagt ttgactaccg agtatctggg
     3481 ttaaagggca tttgctgcaa atgcaaggag cctatcacct taaccagcag ggagaacggt
     3541 catgaggcag cgtgtactgt ttcaaactgg cttgctggcc atgaatctaa acctctgcca
     3601 aatcttccta aaagctaccg atggggttta gttcattggt ggatgggtat taaagatagc
     3661 gagttcgatc acttttcgtt cgttcaattt ttctcaaact ggccaaggtc attccactcg
     3721 ataatcgaag atgaagtaga gttcaacctt gagcatgctg ttgtcagcac gtctgaatta
     3781 cgactaaaag atcttcttgg tcgattgttt ttcggttcaa ttcggttacc tgagcggaat
     3841 cttcaacaca atatcatcct tggtgagctt ctctgctatt tagaaaatcg tttatggcaa
     3901 gacaagggat taatcgccaa cctcaaaatg aacgcgttag aggcgactgt aatgttaaat
     3961 tgtagcctcg atcagattgc atcaatggtt gaacaacgca tcttgaagcc aaatcgaaaa
     4021 agcaagccca acagccctct tgatgttacc gattatctat ttcatttcgg cgatattttc
     4081 tgtctttggt tagctgagtt ccaaagcgat gagtttaacc gttcgtttta tgtgtcgagg
     4141 tggtaaatgc aaactctgaa agaactaatc gcatccaatc ctgacgactt aacaactgaa
     4201 cttaagagag catttcgtcc actcacaccg catattgcaa ttgatggtaa tgaacttgac
     4261 gcactgacga tattagtcaa tttaaccgat aagactgatg atcagaaaga cctgctcgat
     4321 cgagccaaat gcaagcaaaa acttcgagat gaaaaatggt gggctagctg cataaattgc
     4381 gttaattaca gacaaagcca taacccaaaa ttcccggata tacgttctga aggcgtgatt
     4441 cgaacccaag ccctgggtga attaccgagt ttcctactct cgtcctcaaa aatcccacca
     4501 taccattggt catatagcca tgattcaaaa tacgtcaaca aaagcgcatt cctcaccaat
     4561 gagttttgtt gggatggtga gatctcatgt ttgggtgagc ttcttaaaga tgcagatcac
     4621 ccactttgga atactttgaa aaagttaggt tgttctcaaa aaacttgcaa agcaatggca
     4681 aaacaactag ctgatattac tctcacgact atcaatgtca cactcgcacc aaattacctg
     4741 actcaaatat ctctccccga tagtgataca tcctacattt cactctctcc tgttgcatcg
     4801 ctatcgatgc aaagccactt tcatcagagg ctccaagatg aaaatcggca tagcgcgata
     4861 acgcggttta gccgaactac caacatgggg gtcactgcga tgacatgtgg cggcgcattt
     4921 agaatgttaa agtctggcgc taagttttcc agcccccctc atcaccgatt aaacagtaaa
     4981 cgaagttggt tgacgtcaga gcatgttcag tcattaaaac agtaccagcg cctcaacaaa
     5041 agcctcatac ctgaaaactc tcggattgca ctccgtagaa aatacaaaat cgagcttcaa
     5101 aatatggtca gatcttggtt tgcaatgcaa gaccataccc ttgattcgaa catacttatc
     5161 caacatttga atcatgacct atcttactta ggagccacaa aacgttttgc atacgatcca
     5221 gcgatgacca agctctttac tgagcttttg aaacgagagt tatcaaattc aatcaataat
     5281 ggtgagcaac acactaatgg atcgttttta gtcctaccga atatcagggt ttgtggcgca
     5341 acagctttaa gctccccggt aacggtgggg attccatcac ttacagcttt ctttggcttc
     5401 gttcacgcat ttgaacggaa tataaatcgc accacctcat cgtttcgtgt tgaatccttt
     5461 gcgatatgcg tccatcaact acatgtcgag aagcgaggtt tgacagcaga gtttgtggaa
     5521 aaaggcgacg ggactatatc cgctcccgcg acccgggatg actggcagtg tgatgtcgta
     5581 tttagcctta ttttgaacac caactttgct caacatattg accaagatac gttagttaca
     5641 tcactaccaa agcgattggc tcggggttca gcaaaaattg cgattgatga ctttaaacat
     5701 atcaactcat tctcgacatt agaaacagcg atcgaatctc tgccaataga agctggtagg
     5761 tggttatcac tttacgcaca gtcaaacaat aatctaagtg atctattagc agccatgaca
     5821 gaggaccatc agctcatggc aagctgcgtc ggttaccact tgttagaaga gcccaaagat
     5881 aaaccaaact ccctcagagg ttacaaacac gctatcgccg agtgcatcat tggactcatt
     5941 aactcaatca cctttagctc agagactgat cccaacacaa tcttttggtc gctaaagaac
     6001 tatcaaaact acctagtggt acagccaagg agtatcaacg atgaaactac cgacaaatct
     6061 agcctatgag cgctctatcg acccatcaga tgtctgtttt tttgtcgtct ggcccgatga
     6121 tagaaaaaca cctttaacct acaattctcg tactctgctc gggcaaatgg aagcggcatc
     6181 attagcctat gatgtctcag gtcaaccaat aaaaagtgcc accgctgagg cgttagctca
     6241 agggaaccct catcaagttg atttctgcca cgttccatac ggtgcgagtc atattgaatg
     6301 cagtttctcc gtctcgtttt cttctgaact acgtcaacca tataagtgta actcaagcaa
     6361 agttaaacaa acgctagtgc aattagtcga gctctacgaa acgaaaatcg gctggactga
     6421 gctagcaacc cgatatttga tgaacatttg caacggtaaa tggctgtgga aaaatacccg
     6481 taaagcctat tgctggaaca ttgtacttac accttggcct tggaacgggg aaaaggttgg
     6541 atttgaagat attcgtacta actacacctc acggcaagac tttaaaaata ataaaaattg
     6601 gtctgctata gttgaaatga tcaaaaccgc attttctagt actgatgggc tggcgatatt
     6661 tgaagtcagg gccaccttgc acttgccaac gaatgctatg gtgcggccaa gccaagtttt
     6721 cacagaaaaa gaaagtggca gtaaaagtaa atctaaaact caaaacagtc gagtttttca
     6781 gagtacaact attgatggtg aacgatcgcc aatactaggg gcctttaaaa cgggagcagc
     6841 tattgcaacc attgacgact ggtatcctga agccactgag ccactaaggg tcggacggtt
     6901 tggggttcat cgcgaagatg tcacttgcta ccgtcatccg tctaccggaa aagatttttt
     6961 ctcgatatta caacaagcag agcactatat tgaagtgttg agcgccaaca aaactcccgc
     7021 tcaagaaact atcaacgaca tgcacttttt aatggctaac ctgattaagg gtgggatgtt
     7081 ccagcataaa ggagactgac tgtgaaatgg tattataaga caatcacctt tctgccagag
     7141 ttgtgcaaca acgagtcact ggctgcaaag tgtctccgcg ttctgcatgg atttaactat
     7201 cagtatgaga cacgaaatat cggcgtttca tttccgcttt ggtgtgatgc aacggttgga
     7261 aaaaagattt catttgtcag caagaacaag atagaactcg acttactact taaacaacac
     7321 tatttcgtcc aaatggaaca acttcaatac tttcatatat ccaacactgt tctcgtccca
     7381 gaagattgta catacgtttc ctttagacgc tgtcaatcta tagataagct cacagcagca
     7441 gggctggcaa ggaaaatcag acgcctggag aaacgtgctc tatctagagg cgagcaattt
     7501 gacccatcat cttttgctca aaaagagcat actgcaatag cgcactacca ctcacttggg
     7561 gagtccagca aacagacgaa ccgcaacttt cgactcaata tcaggatgct ctcggagcaa
     7621 ccccgtgagg ggaactcgat ttttagtagc tatggcttat caaattcaga aaactcgttt
     7681 cagcctgtac ccttaatctg atctagaaaa ggaggaagga tcataccatg gcgacaagtt
     7741 tacctacgcc ctcagcaatt acgacttcgg cgttagagta tgcattccat actcccgctc
     7801 gcaatctaac gaaatctcgc ggaaaaaaca ttcatcgtta tgtcagtgta aagatgagta
     7861 agaggattac ggtagaatct actctagagt gtgatgcctg ctatcacttt gattttgagc
     7921 caagtattgt tcgcttttgc gctcaaccga ttcgattttt atattatctc aatggtcagt
     7981 ctcactccta tgttcctgac tttctagttc aatttgatac caacgagttt gttctatatg
     8041 aagtaaagtc agcttatgct aagaacaaac ctgattttga tgttgaatgg gaggcgaaag
     8101 taaaagcagc aactgaacta gggctagaat tggagcttgt tgaagagagt gatattaggg
     8161 atacggttgt attaaataat cttaagcgca tgcatcgtta tgcttcgaaa gatgagctga
     8221 ataacgtaca taactctctc ttaaaaataa taaagtacaa tggcgcccaa tctgcaagat
     8281 gcttgggaga acagttgggt ttaaaaggcc gaactgtttt accaattttg tgcgatttgc
     8341 tgtcaaggtg tttactcgat acacgtttgg ataagcctct atctcttgaa tctcgatttg
     8401 agttggccag ttatggctaa gaaagggttc tcaagtttcc atagaaaagc agtctcttcc
     8461 caagatacgc ttgaatcgat agagcttgtc tctagcgcta attgtctaga aagtgttacg
     8521 tatcaagata tatcagcatt tcccgaaaca attgcggtag agattaattt ccgattaagc
     8581 attcttcgct ttttagcgcg aaagtgcgaa accattgtgg ccaaatcaat tgaaccacat
     8641 cgtgtagagc tacagcaaaa ctatagtaga aaaataccca gtgcaataac gatatatcga
     8701 tggtggcttg cttttcgaaa atcagactac aaccccatta gcttagcacc taatatcaag
     8761 gatagaggta atagagagac aaaagtgtca acagttgttg attctattat ggaacaggca
     8821 gttgaaagag ttatatctgg acgaaaagtc aatgttagct ctgcatataa acgtgttcga
     8881 cgaaaagttc gtcaatacaa tctaactcat ggaacgaaat acacgtatcc taagtacgaa
     8941 tctgtaagaa agcgagtaaa aaagaaaacc ccatttgagt tattagccgc agggaaaggg
     9001 gagagagtag ctaagagaga gtttcgccga atgggaaaaa agatcctcac gtctagcgtg
     9061 ctagagaggg ttgaaataga tcacactgtc gttgaccttt ttgcagtaca cgaagagtat
     9121 cgaatcccat tgggccgacc ttggcttact caattggttg attgttacag taaagctgta
     9181 atcggttttt atttaggttt cgagcctcct agctatgtgt cggtttccct tgcacttaag
     9241 aatgcaatac aacgcaaaga tgacttaatc tcctcgtatg aatcgatcga gaatgaatgg
     9301 ctatgttatg gcatcccaga cctactcgta actgataatg gtaaagagtt tttgtcgaaa
     9361 gcatttgatc aagcatgtga atcactattg atcaatgtgc atcaaaataa agttgagacg
     9421 cccgacaaca aacctcatgt tgaacgtaac tacgggacta ttaatacttc tctgttagac
     9481 gatttacctg ggaaatcctt cagccagtac cttcaaagag aagggtacga ctctgtggga
     9541 gaagctaccc ttacactcaa tgagattaga gaaatttact taatttggtt ggtggatatt
     9601 tatcataaaa aacccaatca gagaggcact aattgtccta atgttgcgtg gaaaaagggt
     9661 tgtcaagaat gggaaccaga ggagttctct ggttctaaag acgaattaga ctttaaattt
     9721 gctattgttg attacaaaca acttactaaa gtagggataa ctgtctacaa agaactgagt
     9781 tatagcaatg accgtttagc tgaatataga gggaagaaag gaaaccataa agttcagttc
     9841 aagtataacc ctgagtgtat ggcagttatt tgggtgttgg atgaggatat gaatgagtac
     9901 tttacagtta atgcgattga ctacgaatat gcaagtagag tatcactttg gcaacataaa
     9961 tataacatga aatatcaagc agaactaaat tcagcagaat atgatgagga caaggaaatt
    10021 gatgcagaaa taaaaattga agaaatcgca gatcgttcaa ttgttaagac taacaaaatc
    10081 agagctcgga ggcgtggcgc taggcatcaa gagaatagcg caagggctaa gtcaatcagt
    10141 aatgcgaacc cggcctcgat acaaaaacat gaagatgaaa tcgttagtgc agataatgac
    10201 gattgggata ttgattatgt ctgagaaatc gtcaaatgag tgaaacgcgt gaggctcgta
    10261 tatcaagagc taaaagggca tttgtatcca caccgagcgt taggaaaatc ttgagttaca
    10321 tggatagatg tagagatcta tcagacctag agtctgagcc tacatgcatg atggtctatg
    10381 gtgcttcggg tgtaggtaaa acgaccgtca tcaagaaata cttaaatcaa aacagaagag
    10441 agtccgaagc cgggggcgat ataataccgg ttttgcatat tgagttgcca gacaatgcga
    10501 agccagtaga tgcagcaagg gaattgctgg ttgaaatggg tgacccgcta gcactttatg
    10561 aaactgactt agctagattg acgaaaagac tgactgaatt aatccctgcg gtcggcgtga
    10621 agctgattat tatcgatgag ttccaacatt tggtggaaga aaggtcaaat cgggttctta
    10681 cccaagtagg taattggcta aaaatgatac ttaacaaaac gaaatgtcca attgttatat
    10741 ttggtatgcc atactcaaaa gttgtactgc aagcaaactc gcaacttcac gggcgatttt
    10801 ccattcaggt tgaactgcgc ccctttagct accagggagg tagaggtgta tttaaaactt
    10861 ttttggaata ccttgataaa gccctacctt ttgaaaaaca ggctggctta gccaacgaaa
    10921 gtttgcagaa aaaattgtat gcattctctc agggaaacat gcgttcgttg agaaacctta
    10981 tttatcaagc atctatcgaa gcaattgata atcagcatga gacgataacc gaagaagatt
    11041 tcgtttttgc atcgaagttg acatcgggcg ataaacccaa ctcatggaaa aatccttttg
    11101 aggagggtgt tgaggtaaca gaagatatgt tacgaccgcc accaaaagat attggttggg
    11161 aggactattt gagacattca accccgagag tgagtaaacc aggtagaaat aaaaactttt
    11221 tcgaataacc taggtgcaac tgaaaagttt ataccttacc tggaacaaat ggttgaaaca
    11281 tacgaggcta atatcggctt attaggaata gtccctgtac taataaaatc aggtggatca
    11341 gttgatcagt atattttgga cgaagctcgg aaagaatttg gagatgactt gcttaattcc
    11401 acaattaaat taagggaaag aataaagcga tttgatgttc aaggaatcac ggaagaagat
    11461 actcatgata aagaagctct aaaactattc aataacctta caatggaatt gatcgaaagg
    11521 gtggaaggtt aatggtacga aaattagggg atctacctag aaagccacaa ggcgataggt
    11581 caagcttaaa gaacccttac atggatctta cagattctga aagtaaagaa acaacagagg
    11641 ttaaacaaac agaaccaaaa agaaaaaaag cattgttgaa aacaatgaaa gttgatgttt
    11701 caatccataa taagattaaa tcgctgcacg aaattctggc agcatccgaa gggaattcat
    11761 attacttaga ggatactatt gagagagcta ttgataagat ggttgagaca ttacctgaga
    11821 gccaaaaaac tttttatgaa tatgaattaa aaaaaagaac caacaaaggc tgagacagac
    11881 tccaaacgag tctgtttttt taaaaaaaat attaggagca ttgaatatat attagagaat
    11941 taagaaagac atgggaataa aaatatttta aatccagtaa aaatatgata agattatttc
    12001 agaatatgaa gaactctgtt tgtttttgat gaaaaaacaa acaaaaaaaa tccacctaac
    12061 ggaatctcaa tttaactaac agcggccaaa ctgagaagtt aaatttgaga aggggaaaag
    12121 gcggatttat acttgtattt aactatctcc attttaacat tttattaaac cccatacaag
    12181 tgaaaatcct cttttacact gttcctttag gtgatcgcgg agggacatta tgagtgaagt
    12241 aaacctaaaa ggaaatacag atgaattagt gtattatcga cagcaaacca ctggaaataa
    12301 aatcgccagg aagagaatca aaaaagggaa agaagaagtt tattatgttg ctgaaacgga
    12361 agagaagata tggacagaag agcaaataaa aaacttttct ttagacaaat ttggtacgca
    12421 tataccttac atagaaggtc attatacaat cttaaataat tacttctttg atttttgggg
    12481 ctatttttta ggtgctgaag gaattgcgct ctatgctcac ctaactcgtt atgcatacgg
    12541 cagcaaagac ttttgctttc ctagtctaca aacaatcgct aaaaaaatgg acaagactcc
    12601 tgttacagtt agaggctact tgaaactgct tgaaaggtac ggttttattt ggaaggtaaa
    12661 cgtccgtaat aaaaccaagg ataacacaga ggaatccccg atttttaaga ttagacgtaa
    12721 ggttcctttg ctttcagaag aacttttaaa tggaaaccct aatattgaaa ttccagatga
    12781 cgaggaagca catgtaaaga aggctttaaa aaaggaaaaa gagggtcttc caaaggtttt
    12841 gaaaaaagag cacgatgaat ttgttaaaaa aatgatggat gagtcagaaa caattaatat
    12901 tccagaggcc ttacaatatg acacaatgta tgaagatata ctcagtaaag gagaaattcg
    12961 aaaagaaatc aaaaaacaaa tacctaatcc tacaacatct tttgagagta tatcaatgac
    13021 aactgaagag gaaaaagtcg acagtacttt aaaaagcgaa atgcaaaatc gtgtctctaa
    13081 gccttctttt gatacctggt ttaaaaacac taagatcaaa attgaaaata aaaattgttt
    13141 attacttgta ccgagtgaat ttgcatttga atggattaag aaaagatatt tagaaacaat
    13201 taaaacagtc cttgaagaag ctggatatgt tttcgaaaaa atcgaactaa gaaaagtgca
    13261 ataaactgct gaagtatttc agcagttttt tttatttaga aatagtgaaa aaaatataat
    13321 cagggaggta tcaatattta atgagtactg atttaaattt atttagactg gaattaataa
    13381 ttaacacgta gactaattaa aatttaatga gggataaaga ggatacaaaa atattaattt
    13441 caatccctat taaattttaa caaggggggg attaaaattt aattagaggt ttatccacaa
    13501 gaaaagaccc taataaaatt tttactaggg ttataacact gattaatttc ttaatggggg
    13561 agggattaaa atttaatgac aaagaaaaca atcttttaag aaaagctttt aaaagataat
    13621 aataaaaaga gctttgcgat taagcaaaac tctttacttt ttcattgaca ttatcaaatt
    13681 catcgatttc aaattgttgt tgtatcataa agttaattct gttttgcaca accttttcag
    13741 gaatataaaa cacatctgag gcttgtttta taaactcagg gtcgctaaag tcaatgtaac
    13801 gtagcatatg atatggtata gcttccaccc aagttagcct ttctgcttct tctgaatgtt
    13861 tttcatatac ttccatgggt atctctaaat gattttcctc atgtagcaag gtatgagcaa
    13921 aaagtttatg gaattgatag ttcctctctt tttcttcaac ttttttatct aaaacaaaca
    13981 ctttaacatc tgagtcaatg taagcataag atgtttttcc agtcataatt tcaatcccaa
    14041 atcttttaga cagaaattct ggacgtaaat cttttggtga aagaattttt ttatgtagca
    14101 atatatccga tacagcacct tctaaaagcg ttggtgaata gggcatttta cctatctcct
    14161 ctcattttgt ggaataaaaa tagtcatatt cgtccatcta cctatcctat tatcgaacag
    14221 ttgaactttt taatcaagga tcagtccttt ttttcattat tcttaaactg tgctcttaac
    14281 tttaacaact cgatttgttt ttccagatct cgagggtaac tagcctcgcc gatcccgcaa
    14341 gaggcccggc agtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    14401 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    14461 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    14521 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    14581 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    14641 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    14701 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
    14761 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    14821 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    14881 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    14941 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    15001 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    15061 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    15121 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    15181 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    15241 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    15301 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    15361 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    15421 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    15481 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    15541 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    15601 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    15661 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    15721 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    15781 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    15841 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    15901 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    15961 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    16021 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    16081 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    16141 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    16201 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    16261 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca tgc
//