| 质粒名称: | pAG426GPD-ccdB |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 14156 |
| 存储实验室: | Susan Lindquist |
|
载体骨架基本信息 |
|
| 载体名称: | pRS426 |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素和氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达；Gateway Destination |
| 筛选标记: | URA3 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | GPDPro_F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pAG426GPD-ccdB was a gift from Susan Lindquist (Addgene plasmid # 14156 ; http://n2t.net/addgene:14156 ; RRID:Addgene_14156) |
买家导航
pAG426GPD-ccdB载体 pAG426GPDccdB质粒基本信息
pAG426GPD-ccdB质粒图谱 pAG426GPDccdB载体图谱和pAG426GPD-ccdB载体序列 pAG426GPDccdB质粒序列多克隆位点信息
pAG426GPD-ccdB质粒 pAG426GPDccdB载体简介
pAG426GPD-ccdB质粒序列 pAG426GPDccdB载体序列
LOCUS pAG426GPD-ccdB 8306 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAG426GPD-ccdB
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8306)
TITLE S cerevisiae Advanced Gateway Destination Vectors
REFERENCE 2 (bases 1 to 8306)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8306
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(10..28)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
rep_origin 69..1411
/label=2u ori
/note="yeast 2u plasmid origin of replication"
protein_bind 401..448
/label=FRT
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="FLP-mediated recombination occurs in the 8-bp core
sequence TCTAGAAA (Turan and Bode, 2011)."
promoter 1438..1542
/gene="bla"
/label=AmpR promoter
CDS 1543..2403
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1761..1780)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2574..3162
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3063..3082
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3316..3333
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 3450..3471
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 3486..3516
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3524..3540
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 3529..3551
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 3548..3564
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 3548..3564
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind 3583..3603
/label=T3
/note="T3 promoter, forward primer"
promoter 3585..3603
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 3628..4271
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase"
primer_bind 4165..4188
/label=GPDpro-F
/note="S. cerevisiae GPD promoter, forward primer"
primer_bind 4277..4293
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 4277..4293
/label=pBluescriptSK
/note="For pBluescript vector"
protein_bind 4301..4425
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 4450..4480
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 4534..5193
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(4600..4619)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 5535..5840
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 5719..5738
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(5881..6005)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
primer_bind complement(6038..6054)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6039..6055)
/label=pBluescriptKS
/note="For pBluescript vector"
terminator 6057..6304
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(6073..6091)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
primer_bind complement(6322..6341)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(6323..6341)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6351..6368)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6351..6367)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6360..6382)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 6508..6963
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6595..6614)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6805..6826
/label=F1ori-F
/note="F1 origin, forward primer"
CDS complement(7094..7897)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(7898..8113)
/gene="S. cerevisiae URA3"
/label=URA3 promoter
primer_bind complement(8137..8156)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8256..8278
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttagtatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
121 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
181 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
241 gcatataagt acgcatttaa gcataaacac gcactatgcc gttcttctca tgtatatata
301 tatacaggca acacgcagat ataggtgcga cgtgaacagt gagctgtatg tgcgcagctc
361 gcgttgcatt ttcggaagcg ctcgttttcg gaaacgcttt gaagttccta ttccgaagtt
421 cctattctct agaaagtata ggaacttcag agcgcttttg aaaaccaaaa gcgctctgaa
481 gacgcacttt caaaaaacca aaaacgcacc ggactgtaac gagctactaa aatattgcga
541 ataccgcttc cacaaacatt gctcaaaagt atctctttgc tatatatctc tgtgctatat
601 ccctatataa cctacccatc cacctttcgc tccttgaact tgcatctaaa ctcgacctct
661 acatttttta tgtttatctc tagtattact ctttagacaa aaaaattgta gtaagaacta
721 ttcatagagt gaatcgaaaa caatacgaaa atgtaaacat ttcctatacg tagtatatag
781 agacaaaata gaagaaaccg ttcataattt tctgaccaat gaagaatcat caacgctatc
841 actttctgtt cacaaagtat gcgcaatcca catcggtata gaatataatc ggggatgcct
901 ttatcttgaa aaaatgcacc cgcagcttcg ctagtaatca gtaaacgcgg gaagtggagt
961 caggcttttt ttatggaaga gaaaatagac accaaagtag ccttcttcta accttaacgg
1021 acctacagtg caaaaagtta tcaagagact gcattataga gcgcacaaag gagaaaaaaa
1081 gtaatctaag atgctttgtt agaaaaatag cgctctcggg atgcattttt gtagaacaaa
1141 aaagaagtat agattctttg ttggtaaaat agcgctctcg cgttgcattt ctgttctgta
1201 aaaatgcagc tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgtttt
1261 acaaaaatga agcacagatt cttcgttggt aaaatagcgc tttcgcgttg catttctgtt
1321 ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg ttgcattttt
1381 gttctacaaa atgaagcaca gatgcttcgt tcaggtggca cttttcgggg aaatgtgcgc
1441 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
1501 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
1561 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
1621 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
1681 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
1741 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
1801 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
1861 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
1921 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
1981 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
2041 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
2101 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
2161 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
2221 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
2281 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
2341 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
2401 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
2461 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
2521 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
2581 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
2641 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
2701 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
2761 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
2821 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
2881 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
2941 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
3001 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
3061 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
3121 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
3181 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
3241 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
3301 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
3361 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
3421 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
3481 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
3541 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa
3601 agggaacaaa agctggagct cagtttatca ttatcaatac tcgccatttc aaagaatacg
3661 taaataatta atagtagtga ttttcctaac tttatttagt caaaaaatta gccttttaat
3721 tctgctgtaa cccgtacatg cccaaaatag ggggcgggtt acacagaata tataacatcg
3781 taggtgtctg ggtgaacagt ttattcctgg catccactaa atataatgga gcccgctttt
3841 taagctggca tccagaaaaa aaaagaatcc cagcaccaaa atattgtttt cttcaccaac
3901 catcagttca taggtccatt ctcttagcgc aactacagag aacaggggca caaacaggca
3961 aaaaacgggc acaacctcaa tggagtgatg caacctgcct ggagtaaatg atgacacaag
4021 gcaattgacc cacgcatgta tctatctcat tttcttacac cttctattac cttctgctct
4081 ctctgatttg gaaaaagctg aaaaaaaagg ttgaaaccag ttccctgaaa ttattcccct
4141 acttgactaa taagtatata aagacggtag gtattgattg taattctgta aatctatttc
4201 ttaaacttct taaattctac ttttatagtt agtctttttt ttagttttaa aacaccagaa
4261 cttagtttcg acggattcta gaactagtgg atcccccatc acaagtttgt acaaaaaagc
4321 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
4381 acagactaca taatactgta aaacacaaca tatccagtca ctatggcggc cgcattaggc
4441 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
4501 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
4561 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
4621 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
4681 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
4741 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
4801 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
4861 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
4921 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
4981 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
5041 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
5101 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
5161 cagtactgcg atgagtggca gggcggggcg taaacgcgtg gatccggctt actaaaagcc
5221 agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata tactgatatg
5281 tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca gtgacagttg
5341 acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg gtctggtaag
5401 cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag cggaaaatca
5461 ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg ctgacgagaa
5521 caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc cgttatcgtc
5581 tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg gtgatccccc
5641 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata
5701 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta
5761 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc
5821 tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac
5881 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
5941 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa
6001 gtggtgatgg gctgcaggaa ttcgatatca agcttatcga taccgtcgac ctcgagtcat
6061 gtaattagtt atgtcacgct tacattcacg ccctcccccc acatccgctc taaccgaaaa
6121 ggaaggagtt agacaacctg aagtctaggt ccctatttat ttttttatag ttatgttagt
6181 attaagaacg ttatttatat ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc
6241 atgtaacatt atactgaaaa ccttgcttga gaaggttttg ggacgctcga aggctttaat
6301 ttgcggccgg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
6361 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
6421 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
6481 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg
6541 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
6601 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
6661 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
6721 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
6781 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
6841 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
6901 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
6961 tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatagggta
7021 ataactgata taattaaatt gaagctctaa tttgtgagtt tagtatacat gcatttactt
7081 ataatacagt tttttagttt tgctggccgc atcttctcaa atatgcttcc cagcctgctt
7141 ttctgtaacg ttcaccctct accttagcat cccttccctt tgcaaatagt cctcttccaa
7201 caataataat gtcagatcct gtagagacca catcatccac ggttctatac tgttgaccca
7261 atgcgtctcc cttgtcatct aaacccacac cgggtgtcat aatcaaccaa tcgtaacctt
7321 catctcttcc acccatgtct ctttgagcaa taaagccgat aacaaaatct ttgtcgctct
7381 tcgcaatgtc aacagtaccc ttagtatatt ctccagtaga tagggagccc ttgcatgaca
7441 attctgctaa catcaaaagg cctctaggtt cctttgttac ttcttctgcc gcctgcttca
7501 aaccgctaac aatacctggg cccaccacac cgtgtgcatt cgtaatgtct gcccattctg
7561 ctattctgta tacacccgca gagtactgca atttgactgt attaccaatg tcagcaaatt
7621 ttctgtcttc gaagagtaaa aaattgtact tggcggataa tgcctttagc ggcttaactg
7681 tgccctccat ggaaaaatca gtcaagatat ccacatgtgt ttttagtaaa caaattttgg
7741 gacctaatgc ttcaactaac tccagtaatt ccttggtggt acgaacatcc aatgaagcac
7801 acaagtttgt ttgcttttcg tgcatgatat taaatagctt ggcagcaaca ggactaggat
7861 gagtagcagc acgttcctta tatgtagctt tcgacatgat ttatcttcgt ttcctgcagg
7921 tttttgttct gtgcagttgg gttaagaata ctgggcaatt tcatgtttct tcaacactac
7981 atatgcgtat atataccaat ctaagtctgt gctccttcct tcgttcttcc ttctgttcgg
8041 agattaccga atcaaaaaaa tttcaaggaa accgaaatca aaaaaaagaa taaaaaaaaa
8101 atgatgaatt gaaaaggtgg tatggtgcac tctcagtaca atctgctctg atgccgcata
8161 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
8221 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
8281 ttcaccgtca tcaccgaaac gcgcga
//
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pAG426GPD-ccdB质粒 pAG426GPDccdB载体应用举例
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