| 质粒名称: | pAAVLINK-EFS-HA-5'-ANKRD11 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001924 |
| 产品用途: | 5' AAVLINK plasmid for ANKRD11 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ANKRD11 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-5'-ANKRD11
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-5'-ANKRD11载体质粒基本信息
pAAVLINK-EFS-HA-5'-ANKRD11质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-ANKRD11载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-ANKRD11质粒载体简介
pAAVLINK-EFS-HA-5'-ANKRD11质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-ANKRD11 7264 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7264)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7264
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 371..397
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 404..4404
/codon_start=1
/gene="ANKRD11"
/gene_synonym="ANCO-1; ANCO1; LZ16; T13"
/product="ankyrin repeat domain-containing protein 11"
/label=ANKRD11
/note="nasopharyngeal carcinoma susceptibility
protein;ankyrin repeat-containing cofactor 1; ankyrin
repeat domain 11; ankyrin repeats containing cofactor 1"
/db_xref="GI:56676397"
/db_xref="CCDS:CCDS32513.1"
/db_xref="GeneID:29123"
/db_xref="HGNC:HGNC:21316"
/db_xref="MIM:611192"
/protein_id="NP_037407.4"
/translation="PKGGCPKAPQQEELPLSSDMVEKQTGKKDKDKVSLTKTPKLERGD
GGKEVRERASKRKLPFTAGANGEQKDSDTEKQGPERKRIKKEPVTRKAGLLFGMGLSGI
RAGYPLSERQQVALLMQMTAEESANSPVDTTPKHPSQSTVCQKGTPNSASKTKDKVNKR
NERGETRLHRAAIRGDARRIKELISEGADVNVKDFAGWTALHEACNRGYYDVAKQLLAA
GAEVNTKGLDDDTPLHDAANNGHYKVVKLLLRYGGNPQQSNRKGETPLKVANSPTMVNL
LLGKGTYTSSEESSTESSEEEDAPSFAPSSSVDGNNTDSEFEKGLKHKAKNPEPQKATA
PVKDEYEFDEDDEQDRVPPVDDKHLLKKDYRKETKSNSFISIPKMEVKSYTKNNTIAPK
KASHRILSDTSDEEDASVTVGTGEKLRLSAHTILPGSKTREPSNAKQQKEKNKVKKKRK
KETKGREVRFGKRSDKFCSSESESESSESGEDDRDSLGSSGCLKGSPLVLKDPSLFSSL
SASSTSSHGSSAAQKQNPSHTDQHTKHWRTDNWKTISSPAWSEVSSLSDSTRTRLTSES
DYSSEGSSVESLKPVRKRQEHRKRASLSEKKSPFLSSAEGAVPKLDKEGKVVKKHKTKH
KHKNKEKGQCSISQELKLKSFTYEYEDSKQKSDKAILLENDLSTENKLKVLKHDRDHFK
KEEKLSKMKLEEKEWLFKDEKSLKRIKDTNKDISRSFREEKDRSNKAEKERSLKEKSPK
EEKLRLYKEERKKKSKDRPSKLEKKNDLKEDKISKEKEKIFKEDKEKLKKEKVYREDSA
FDEYCNKNQFLENEDTKFSLSDDQRDRWFSDLSDSSFDFKGEDSWDSPVTDYRDMKSDS
VAKLILETVKEDSKERRRDSRAREKRDYREPFFRKKDRDYLDKNSEKRKEQTEKHKSVP
GYLSEKDKKRRESAEAGRDRKDALESCKERRDGRAKPEEAHREELKECGCESGFKDKSD
GDFGKGLEPWERHHPAREKEKKDGPDKERKEKTKPERYKEKSSDKDKSEKSILEKCQKD
KEFDKCFKEKKDTKEKHKDTHGKDKERKASLDQGKEKKEKAFPGIISEDFSEKKDDKKG
KEKSWYIADIFTDESEDDRDSCMGSGFKMGEASDLPRTDGLQEKEEGREAYASDRHRKS
SDKQHPERQKDKEPRDRRKDRGAADAGRDKKEKVFEKHKEKKDKESTEKYKDRKDRASV
DSTQDKKNKQKLPEKAEKKHAAEDKAKSKHKEKSDKEHSKERKSSRSADAEKSLLEKLE
EEALHEYREDSNDKISEVSSDSFTDRGQEPGLTAFLEVSFTEPPGDDKP"
misc_feature 546..551
/label=SDS
misc_feature 1243..1248
/label=SDS
misc_feature 1638..1643
/label=SDS
misc_feature 1959..1964
/label=SDS
misc_feature 2271..2276
/label=SDS
misc_feature 3045..3050
/label=SDS
misc_feature 3062..3067
/label=SDS
misc_feature 3306..3311
/label=SDS
misc_feature 3855..3860
/label=SDS
intron 4405..4445
/label=Splicing Donor
misc_feature 4449..4482
/label=lox2272-JT1571
repeat_region 4527..4667
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4527..4656
/label=AAV2 ITR
repeat_region 4528..4668
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4742..5197
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5479..5583
/gene="bla"
/label=AmpR promoter
CDS 5584..6444
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6615..7203
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cgccaccatg tacccttatg acgtacctga ctatgctgga tctcccaagg gtgggtgccc
421 taaagcacca cagcaggaag agcttcccct cagcagcgac atggtggaga agcagactgg
481 gaaaaaggat aaagataaag tttctctaac caagacccca aaactggagc gtggcgatgg
541 cgggaaggag gtgagggagc gagccagcaa gcggaagctg cccttcaccg cgggcgccaa
601 tggggagcag aaggactcgg acacagagaa gcagggccct gagcggaaga ggattaagaa
661 ggagcctgtc acccggaagg ccgggctgct gtttggcatg gggctgtctg gaatccgagc
721 cggctacccc ctctccgagc gccagcaggt ggcccttctc atgcagatga cggccgagga
781 gtctgccaac agcccagtgg acacaacacc aaagcacccc tcccagtcta cagtgtgtca
841 gaagggaacg cccaactctg cctcaaaaac caaagataaa gtgaacaaga gaaacgagcg
901 tggagagacc cgcctgcacc gagccgccat ccgcggggac gcccggcgca tcaaagagct
961 catcagcgag ggggcagacg tcaacgtcaa ggacttcgca ggctggacgg cgctgcacga
1021 ggcctgtaac cggggctact acgacgtcgc gaagcagctg ctggctgcag gtgcggaggt
1081 gaacaccaag ggcctagatg acgacacgcc tttgcacgac gctgccaaca acgggcacta
1141 caaggtggtg aagctgctgc tgcggtacgg agggaacccg cagcagagca acaggaaagg
1201 cgagacgccg ctgaaagtgg ccaactcccc cacgatggtg aacctcctgt taggcaaagg
1261 cacttacact tccagcgagg agagctcgac ggagagctca gaagaggaag acgcaccatc
1321 cttcgcacct tccagttcag tcgacggcaa caacacggac tccgagttcg aaaaaggcct
1381 caagcacaag gccaagaacc cagagccaca gaaggccacg gcccccgtca aggacgagta
1441 tgagtttgat gaggacgacg agcaggacag ggttcctccg gtggacgaca agcacctatt
1501 gaaaaaggac tacagaaaag aaacgaaatc caatagtttt atctctatac ccaaaatgga
1561 ggttaaaagt tacactaaaa ataacacgat tgcaccaaag aaagcgtccc atcgtatcct
1621 gtcagacacg tcggacgagg aggacgcgag tgtcaccgtg gggacaggag agaagctgag
1681 actctcggca catacgatat tgcctggtag taagacacga gagccttcta atgccaagca
1741 gcagaaggaa aaaaataaag tgaaaaagaa gcgaaagaaa gaaacaaaag gcagagaggt
1801 tcgcttcgga aagcggagcg acaagttctg ctcctcggag tcggagagcg agtcctcaga
1861 gagtggggag gatgacaggg actctctggg gagctctggc tgcctcaagg ggtccccgct
1921 ggtgctgaag gacccctccc tgttcagctc cctctctgcc tcctccacct cgtctcacgg
1981 gagctctgcc gcccagaagc agaaccccag ccacacagac cagcacacca agcactggcg
2041 gacagacaat tggaaaacca tttcttcccc ggcttggtca gaggtcagtt ctttatcaga
2101 ctccacaagg acgagactga caagcgagtc tgactactcc tctgagggct ccagtgtgga
2161 atcgctgaag ccagtgagga agaggcagga gcacaggaag cgagcctccc tgtcggagaa
2221 gaagagcccc ttcctgtcca gcgcggaggg cgctgtcccc aaactggaca aggaggggaa
2281 agttgtcaaa aaacataaaa caaaacacaa acacaaaaac aaggagaagg gacagtgttc
2341 catcagccaa gagctgaagt tgaaaagttt tacttacgaa tatgaggact ccaagcagaa
2401 gtcagataag gctatactgt tagagaatga tctttccact gaaaacaagc taaaagtgtt
2461 aaagcacgat cgcgaccact ttaaaaaaga agagaaactt agcaaaatga aattagaaga
2521 aaaagaatgg ctctttaaag atgaaaaatc actgaagaga atcaaagaca caaacaaaga
2581 catcagcagg tctttccgag aagagaaaga ccgttcgaat aaagcagaaa aggagagatc
2641 gctgaaggaa aagtctccga aagaagaaaa actgagactg tacaaagagg agagaaagaa
2701 gaaatcaaaa gaccggccct caaaattaga gaagaagaat gatttaaaag aggacaaaat
2761 ttcaaaagag aaggagaaga tttttaaaga agataaagaa aaactcaaaa aagaaaaggt
2821 ttatagggaa gattctgctt ttgacgaata ttgtaacaaa aatcagtttc tggagaatga
2881 agacaccaaa tttagccttt ctgacgatca gcgagatcgg tggttttctg acttgtccga
2941 ttcatccttt gatttcaaag gggaggacag ctgggactcg ccagtgacag actacaggga
3001 catgaagagc gactctgtgg ccaagctcat cttggagacg gtgaaggagg acagcaagga
3061 gaggaggcgg gacagccggg cccgggagaa gcgagactac agagagccct tcttccgaaa
3121 gaaggacagg gactatttgg ataaaaactc tgagaagagg aaagagcaga ctgaaaagca
3181 taaaagtgtc cctggctacc tttcggaaaa ggacaagaag aggagagagt ccgcagaggc
3241 cgggcgggac agaaaggacg ccctggagag ctgcaaggag cgcagggacg gcagggccaa
3301 gcccgaggag gcgcaccggg aggagctgaa ggagtgtggc tgcgagagtg gcttcaagga
3361 caagtccgac ggcgactttg ggaagggcct ggagccgtgg gaacggcacc acccagcacg
3421 agagaaggag aagaaggatg gccccgataa ggaaaggaag gagaagacaa aaccagaaag
3481 atacaaagag aaatccagtg acaaggacaa aagtgagaaa tcaatcctgg aaaaatgtca
3541 gaaggacaaa gaatttgata aatgttttaa agagaaaaaa gataccaagg aaaaacataa
3601 agacacacat ggcaaagaca aagaaaggaa agcgtctctc gaccaaggga aagagaagaa
3661 ggagaaggct ttccctggga tcatctcaga agacttctct gaaaaaaaag atgacaagaa
3721 aggcaaagag aaaagctggt acatcgcaga catcttcaca gatgagagtg aggacgacag
3781 agacagctgc atggggagcg ggttcaagat gggagaggcc agcgacttgc cgaggacgga
3841 cggcctccag gagaaggagg aaggacggga ggcctatgcc tccgacagac acaggaagtc
3901 ttctgacaag cagcaccctg agaggcagaa ggacaaggag cccagagaca ggagaaagga
3961 ccgaggggct gccgacgcgg ggagagacaa aaaagagaaa gtctttgaaa agcacaagga
4021 gaagaaggat aaagagtcca cagaaaagta caaggacagg aaggacagag cctcagtgga
4081 ctccacgcaa gataagaaaa ataaacagaa gctccccgag aaggctgaaa agaagcacgc
4141 tgccgaagac aaggctaaaa gcaaacacaa agagaagtcg gacaaagaac attccaagga
4201 gaggaagtcc tcgagaagtg ccgacgcgga aaaaagcctg cttgaaaagt tggaagaaga
4261 ggctctccat gagtacagag aagactccaa cgataaaatc agcgaggtct cctctgacag
4321 cttcacggac cgagggcagg agccggggct gactgccttc ctggaggtct ctttcacgga
4381 gccacctgga gacgacaagc cgaggtaagt atcaaggtta caagacaggt ttaaggagac
4441 caataagcta ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt
4501 gtggcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc
4561 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
4621 tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg
4681 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag
4741 tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
4801 gctacacttg ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
4861 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
4921 agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg
4981 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
5041 ggactcttgt tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta
5101 taagggattt tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt
5161 aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc
5221 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
5281 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
5341 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
5401 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
5461 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
5521 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
5581 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
5641 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
5701 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
5761 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
5821 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
5881 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
5941 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
6001 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
6061 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
6121 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
6181 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
6241 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
6301 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
6361 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
6421 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
6481 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
6541 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
6601 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
6661 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
6721 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
6781 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
6841 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
6901 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
6961 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
7021 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
7081 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
7141 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
7201 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
7261 atgt
//
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