pAAVLINKv1-pCALM1-Cre-BRCA2-C-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-BRCA2-C-FLAG            7197 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7197)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7197
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3910
                     /codon_start=1
                     /label=BRCA2
                     /translation="VWIQSLFLEFGFIITIDGSYGNWQLWNVPFLRNLLIDA*AQKGCF
                     FN*NTDMIRKLIEAEDRL*KR*WKGMTQLQKHLFSVFLT*FH*AQIYLKLLAIKLVVQI
                     PKKWPLLNLQMGGMLLRPS*ILPS*LS*RMAD*QLVRRLFFMEQNWWALLMPVHLLKPQ
                     NLLC*RFLLTVLGLLAGIPNLGSFLTLDLFLCPYHRFSVMEEMLVVLM*LFKEHTLYSG
                     WRRHHLDYTYFAMKERKKRKQQNMWRPNKRD*KPYSLKFRRNLKNMKKTQQNHIYHHVH
                     *QDSKFVLCKMVQSFMKQ*RMQQTQLTLRVISVKSS*EP*IITGKC*MIRNKLRSSWKL
                     GRPWNLLNKRNKVYQGMSQPCGSCVL*AIQKKKKIQLY*VFGVHHQIYILC*QKERDTE
                     FIILQLQNLKVNLKELTYS*QRQKKLSINNYRFQMKFYFRFTSHGSPFTSANF*IQTFS
                     HLVLRWT**DLSFLL*KKQDLPLSSICQTNVTIYWQ*SFG*TLMRTLLSLIC*LLQATS
                     SGDQNPNQAFLLYLLEIFLCFLLVQKRATFKRHSTK*KILLRILTYFAMKQKTSLCIYC
                     MQMIPSGPPQLKTVLQGRTLLKSFLVQETSF*CLLLIVRYIIKVLYHFVWPKGSLFPHL
                     SQPR*LQSLVKGRKRLMTKRTAKREEPWIS*VDCLYLHLLVPFVHLFLRLHRRHFSHQG
                     VVAPNTKHP*RKKN*ILLR*LHLKNSMKFLFWKVIQ*LTKNLH**IPKLFCLVQQEKNN
                     LYLSVNPLGLLPPVQKIISD*NDVVLHL*SKNRRVPRPVRKNVRKISRTQLQLKNI"
     misc_feature    2265..2270
                     /label=SDS
     CDS             3917..3982
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3986..4417
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4130..4135
                     /label=SDS
     repeat_region   4460..4600
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4460..4589
                     /label=AAV2 ITR
     rep_origin      4675..5130
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5412..5516
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5517..6377
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6548..7136
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtggatc caaagcttat
     1501 ttctagaatt tgggtttata atcactatag atggatcata tggaaactgg cagctatgga
     1561 atgtgccttt cctaaggaat ttgctaatag atgcctaagc ccagaaaggg tgcttcttca
     1621 actaaaatac agatatgata cggaaattga tagaagcaga agatcggcta taaaaaagat
     1681 aatggaaagg gatgacacag ctgcaaaaac acttgttctc tgtgtttctg acataatttc
     1741 attgagcgca aatatatctg aaacttctag caataaaact agtagtgcag atacccaaaa
     1801 agtggccatt attgaactta cagatgggtg gtatgctgtt aaggcccagt tagatcctcc
     1861 cctcttagct gtcttaaaga atggcagact gacagttggt cagaagatta ttcttcatgg
     1921 agcagaactg gtgggctctc ctgatgcctg tacacctctt gaagccccag aatctcttat
     1981 gttaaagatt tctgctaaca gtactcggcc tgctcgctgg tataccaaac ttgggttctt
     2041 tcctgaccct agaccttttc ctctgccctt atcatcgctt ttcagtgatg gaggaaatgt
     2101 tggttgtgtt gatgtaatta ttcaaagagc ataccctata cagtggatgg agaagacatc
     2161 atctggatta tacatatttc gcaatgaaag agaggaagaa aaggaagcag caaaatatgt
     2221 ggaggcccaa caaaagagac tagaagcctt attcactaaa attcaggagg aatttgaaga
     2281 acatgaagaa aacacaacaa aaccatattt accatcacgt gcactaacaa gacagcaagt
     2341 tcgtgctttg caagatggtg cagagcttta tgaagcagtg aagaatgcag cagacccagc
     2401 ttaccttgag ggttatttca gtgaagagca gttaagagcc ttgaataatc acaggcaaat
     2461 gttgaatgat aagaaacaag ctcagatcca gttggaaatt aggaaggcca tggaatctgc
     2521 tgaacaaaag gaacaaggtt tatcaaggga tgtcacaacc gtgtggaagt tgcgtattgt
     2581 aagctattca aaaaaagaaa aagattcagt tatactgagt atttggcgtc catcatcaga
     2641 tttatattct ctgttaacag aaggaaagag atacagaatt tatcatcttg caacttcaaa
     2701 atctaaaagt aaatctgaaa gagctaacat acagttagca gcgacaaaaa aaactcagta
     2761 tcaacaacta ccggtttcag atgaaatttt atttcagatt taccagccac gggagcccct
     2821 tcacttcagc aaatttttag atccagactt tcagccatct tgttctgagg tggacctaat
     2881 aggatttgtc gtttctgttg tgaaaaaaac aggacttgcc cctttcgtct atttgtcaga
     2941 cgaatgttac aatttactgg caataaagtt ttggatagac cttaatgagg acattattaa
     3001 gcctcatatg ttaattgctg caagcaacct ccagtggcga ccagaatcca aatcaggcct
     3061 tcttacttta tttgctggag atttttctgt gttttctgct agtccaaaag agggccactt
     3121 tcaagagaca ttcaacaaaa tgaaaaatac tgttgagaat attgacatac tttgcaatga
     3181 agcagaaaac aagcttatgc atatactgca tgcaaatgat cccaagtggt ccaccccaac
     3241 taaagactgt acttcagggc cgtacactgc tcaaatcatt cctggtacag gaaacaagct
     3301 tctgatgtct tctcctaatt gtgagatata ttatcaaagt cctttatcac tttgtatggc
     3361 caaaaggaag tctgtttcca cacctgtctc agcccagatg acttcaaagt cttgtaaagg
     3421 ggagaaagag attgatgacc aaaagaactg caaaaagaga agagccttgg atttcttgag
     3481 tagactgcct ttacctccac ctgttagtcc catttgtaca tttgtttctc cggctgcaca
     3541 gaaggcattt cagccaccaa ggagttgtgg caccaaatac gaaacaccca taaagaaaaa
     3601 agaactgaat tctcctcaga tgactccatt taaaaaattc aatgaaattt ctcttttgga
     3661 aagtaattca atagctgacg aagaacttgc attgataaat acccaagctc ttttgtctgg
     3721 ttcaacagga gaaaaacaat ttatatctgt cagtgaatcc actaggactg ctcccaccag
     3781 ttcagaagat tatctcagac tgaaacgacg ttgtactaca tctctgatca aagaacagga
     3841 gagttcccag gccagtacgg aagaatgtga gaaaaataag caggacacaa ttacaactaa
     3901 aaaatatatc tgtacagact acaaagacca tgacggtgat tataaagatc atgacatcga
     3961 ctacaaggat gacgatgaca agtaactcga gataatcaac ctctggatta caaaatttgt
     4021 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     4081 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     4141 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4201 acaggggctc ggctgttggg cactgacaat tccgtggtgt ttatttgtga aatttgtgat
     4261 gctattgctt tatttgtaac catctagctt tatttgtgaa atttgtgatg ctattgcttt
     4321 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
     4381 gtttcaggtt cagggggaga tgtgggaggt tttttaaaga tctggagacc ggtgtggcac
     4441 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     4501 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     4561 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt
     4621 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc
     4681 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4741 ttgccagcgc cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4801 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4861 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc
     4921 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     4981 tgttccaaac tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga
     5041 ttttgccgat ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5101 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg
     5161 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5221 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     5281 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     5341 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     5401 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5461 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5521 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5581 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5641 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5701 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5761 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5821 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5881 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5941 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6001 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6061 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6121 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6181 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6241 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6301 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6361 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6421 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6481 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6541 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6601 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6661 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6721 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6781 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6841 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6901 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6961 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7021 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7081 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7141 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//