| 质粒名称: | pQL85 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002512 |
| 产品用途: | Lentivirus vector for ETS*-driven hBax expression (LTR-(GGAA)21-PhCMVmin-hBax-pA-LTR). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pLenti-CMV |
| 表达系统: | 慢病毒表达 |
| 启动子: | Synthetic |
| 拷贝数: | 中拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: |
Stbl3
|
|
插入基因信息 |
|
| 插入基因名称: | hBax |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
pQL85
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pQL85载体质粒基本信息
pQL85质粒图谱载体图谱和pQL85载体序列质粒序列多克隆位点信息
pQL85质粒载体简介
pQL85质粒序列载体序列
LOCUS pQL85 7223 bp DNA circular SYN 08-APR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7223)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 29, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7223
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 835..1015
/label=HIV-1 5 LTR
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1126..1170
/label=HIV-1 psi pack
misc_feature 1222..1227
/label=U6 terminator
misc_feature 1251..1256
/label=U6 terminator
misc_feature 1665..1670
/label=U6 terminator
misc_feature 1684..1917
/label=RRE
misc_feature 2444..2561
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2620..2932
/label=U6 promoter
/note="RNA polymerase III promoter for mouse U6 snRNA (Das
et al., 1988)"
misc_feature 2845..2850
/label=U6 terminator
primer_bind 2949..2965
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2994..3008)
/label=QL146-R
misc_recomb 3007..3040
/label=LoxP
protein_bind 3007..3040
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 3048..3088
/label=QL143-F
misc_feature 3089..3144
/label=GGAA14
misc_feature 3145..3172
/label=GGAA14
misc_feature 3179..3326
/label=hcmvmin
misc_feature 3248..3262
/label=New Feature
primer_bind 3317..3358
/label=QL135-F
CDS 3338..4000
/codon_start=1
/gene="BAX"
/gene_synonym="BCL2L4"
/product="apoptosis regulator BAX isoform 1"
/label=Hus BAX
/note="isoform 1 is encoded by transcript variant 1;
apoptosis regulator BAX; bcl2-L-4; bcl-2-like protein 4;
BCL2-associated X protein omega; Baxdelta2G9; BCL2
associated X protein; Baxdelta2omega; Baxdelta2G9omega"
/db_xref="GeneID:581"
/db_xref="HGNC:HGNC:959"
/db_xref="MIM:600040"
/protein_id="NP_001278357.1"
/translation="MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPEL
ALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGN
FNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWGLPLA
ESLKRLMSLSPGRPPLLLWDAHVADRDHLCGGSAHRLTHHLEEDGLRPPAALDCVFPP"
exon 3338..3371
/gene="BAX"
/gene_synonym="BCL2L4"
/inference="alignment:same species:2.1.0"
misc_feature 3338..3340
/gene="BAX"
/gene_synonym="BCL2L4"
/note="N-acetylmethionine.
/evidence=ECO:0007744|PubMed:19413330,
ECO:0007744|PubMed:25944712; propagated from
UniProtKB/Swiss-Prot (Q07812.1); acetylation site"
exon 3372..3423
/gene="BAX"
/gene_synonym="BCL2L4"
/inference="alignment:same species:2.1.0"
exon 3424..3570
/gene="BAX"
/gene_synonym="BCL2L4"
/inference="alignment:same species:2.1.0"
misc_feature 3512..3556
/gene="BAX"
/gene_synonym="BCL2L4"
/note="propagated from UniProtKB/Swiss-Prot (Q07812.1);
Region: BH3"
exon 3571..3706
/gene="BAX"
/gene_synonym="BCL2L4"
/inference="alignment:same species:2.1.0"
misc_feature 3629..3691
/gene="BAX"
/gene_synonym="BCL2L4"
/note="propagated from UniProtKB/Swiss-Prot (Q07812.1);
Region: BH1"
exon 3707..3811
/gene="BAX"
/gene_synonym="BCL2L4"
/inference="alignment:same species:2.1.0"
primer_bind complement(3983..4024)
/label=QL146-R
primer_bind complement(3983..4004)
/label=QL136-R
misc_recomb 4023..4056
/label=LoxP
protein_bind 4023..4056
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 4112..4700
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(4583..4594)
/label=FactorXa site
primer_bind complement(4703..4719)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
LTR 5229..5409
/label=HIV-1 5 LTR
rep_origin complement(5453..6081)
/direction=LEFT
/label=ColE1 origin
rep_origin complement(5471..6059)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 6011..6016
/label=U6 terminator
misc_feature 6012..6017
/label=U6 terminator
misc_feature 6044..6049
/label=U6 terminator
misc_feature 6045..6050
/label=U6 terminator
CDS complement(6230..7090)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(6233..6892)
/label=AmpR
misc_feature 6642..6647
/label=U6 terminator
misc_feature 7049..7054
/label=U6 terminator
promoter complement(7091..7195)
/gene="bla"
/label=AmpR promoter
promoter complement(7132..7160)
/label=Amp prom
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
1561 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
1621 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
1681 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
1741 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
1801 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
1861 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
1921 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
1981 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
2041 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
2101 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
2161 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
2221 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
2281 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
2341 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
2401 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
2461 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
2521 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
2581 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
2641 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
2701 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
2761 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataagag
2821 atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta
2881 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg
2941 gtgaccctcg aggtcgacgg tatcgataag ctcgcttcac gagattccag caggtcgagg
3001 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccaagcttaa
3061 gcggccgcgg ccagatatac gcgtgtacgg aaggaaggaa ggaaggaagg aaggaaggaa
3121 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aagctagctc
3181 gagctcggta cccgggtcga gtaggcgtgt acggtgggag gcctatataa gcagagctcg
3241 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
3301 acaccgggac cgatccagcc tccgcggccc cgaattcatg gacgggtccg gggagcagcc
3361 cagaggcggg gggcccacca gctctgagca gatcatgaag acaggggccc ttttgcttca
3421 gggtttcatc caggatcgag cagggcgaat ggggggggag gcacccgagc tggccctgga
3481 cccggtgcct caggatgcgt ccaccaagaa gctgagcgag tgtctcaagc gcatcgggga
3541 cgaactggac agtaacatgg agctgcagag gatgattgcc gccgtggaca cagactcccc
3601 ccgagaggtc tttttccgag tggcagctga catgttttct gacggcaact tcaactgggg
3661 ccgggttgtc gcccttttct actttgccag caaactggtg ctcaaggccc tgtgcaccaa
3721 ggtgccggaa ctgatcagaa ccatcatggg ctggacattg gacttcctcc gggagcggct
3781 gttgggctgg atccaagacc agggtggttg ggggctgccc ctggccgagt cactgaagcg
3841 actgatgtcc ctgtctccag gacggcctcc tctcctactt tgggacgccc acgtggcaga
3901 ccgtgaccat ctttgtggcg ggagtgctca ccgcctcact caccatctgg aagaagatgg
3961 gctgaggccc ccagctgcct tggactgtgt ttttcctcca taagaattcg tcgagggacc
4021 taataacttc gtatagcata cattatacga agttatacat gtttaagggt tccggttcca
4081 ctaggtacaa ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga
4141 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
4201 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
4261 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
4321 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
4381 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
4441 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
4501 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
4561 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
4621 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
4681 cctttgggcc gcctccccgc atcgataccg tcgacctcga tcgagaccta gaaaaacatg
4741 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac
4801 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt
4861 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
4921 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact
4981 tccctgattg gcagaactac acaccagggc cagggatcag atatccactg acctttggat
5041 ggtgctacaa gctagtacca gttgagcaag agaaggtaga agaagccaat gaaggagaga
5101 acacccgctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtat
5161 tagagtggag gtttgacagc cgcctagcat ttcatcacat ggcccgagag ctgcatccgg
5221 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
5281 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
5341 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
5401 tctctagcag catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
5461 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
5521 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
5581 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
5641 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
5701 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
5761 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
5821 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
5881 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
5941 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
6001 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
6061 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
6121 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
6181 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
6241 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
6301 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
6361 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
6421 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
6481 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
6541 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
6601 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
6661 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
6721 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
6781 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
6841 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
6901 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
6961 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
7021 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
7081 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
7141 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
7201 ttccccgaaa agtgccacct gac
//
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