pDA083

LOCUS       pDA083               10004 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  eGFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    pDA083
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10004)
  AUTHORS   Ardeljan D, Steranka JP, Liu C, Li Z, Taylor MS, Payer LM, Gorbounov
            M, Sarnecki JS, Deshpande V, Hruban RH, Boeke JD, Fenyo D, Wu PH, 
            Smogorzewska A, Holland AJ, Burns KH
  TITLE     Cell fitness screens reveal a conflict between LINE-1 
            retrotransposition and DNA replication.
  JOURNAL   Nat Struct Mol Biol. 2020 Feb;27(2):168-178. doi: 
            10.1038/s41594-020-0372-1. Epub 2020 Feb 10.
  PUBMED    32042151
REFERENCE   2  (bases 1 to 10004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10004
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      643..652
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             649..1365
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             649..1365
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(694..715)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(955..974)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1302..1323
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1412..1546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1436..1455)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1490..1509
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1689..1711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6038..6056)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6124..6228
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6229..7089
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6447..6466)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7260..7848
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7749..7768
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        8279..8424
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             8467..9261
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8521..8540)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9131..9150
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    9303..9351
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatctctag aagctgggta ccagctgcta gcgctaccgg tcgccaccat ggtgagcaag
      661 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      721 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      781 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      841 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      901 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      961 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1021 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1081 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1141 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1201 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1261 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1321 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtccgg actcagatct
     1381 cgagctcaag cttcgaatag cggccgctaa ggatccagac atgataagat acattgatga
     1441 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     1501 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     1561 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa gcaagtaaaa
     1621 cctctacaaa tgtggtatgg ctgattatga tccggctgcc tcgcgcgttt cggtgatgac
     1681 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     1741 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     1801 gccatgaggt cgactctaga ggatcgatcc ccgccccgga cgaactaaac ctgactacga
     1861 catctctgcc ccttcttcgc ggggcagtgc atgtaatccc ttcagttggt tggtacaact
     1921 tgccaactgg gccctgttcc acatgtgaca cgggggggga ccaaacacaa aggggttctc
     1981 tgactgtagt tgacatcctt ataaatggat gtgcacattt gccaacactg agtggctttc
     2041 atcctggagc agactttgca gtctgtggac tgcaacacaa cattgccttt atgtgtaact
     2101 cttggctgaa gctcttacac caatgctggg ggacatgtac ctcccagggg cccaggaaga
     2161 ctacgggagg ctacaccaac gtcaatcaga ggggcctgtg tagctaccga taagcggacc
     2221 ctcaagaggg cattagcaat agtgtttata aggccccctt gttaacccta aacgggtagc
     2281 atatgcttcc cgggtagtag tatatactat ccagactaac cctaattcaa tagcatatgt
     2341 tacccaacgg gaagcatatg ctatcgaatt agggttagta aaagggtcct aaggaacagc
     2401 gatatctccc accccatgag ctgtcacggt tttatttaca tggggtcagg attccacgag
     2461 ggtagtgaac cattttagtc acaagggcag tggctgaaga tcaaggagcg ggcagtgaac
     2521 tctcctgaat cttcgcctgc ttcttcattc tccttcgttt agctaataga ataactgctg
     2581 agttgtgaac agtaaggtgt atgtgaggtg ctcgaaaaca aggtttcagg tgacgccccc
     2641 agaataaaat ttggacgggg ggttcagtgg tggcattgtg ctatgacacc aatataaccc
     2701 tcacaaaccc cttgggcaat aaatactagt gtaggaatga aacattctga atatctttaa
     2761 caatagaaat ccatggggtg gggacaagcc gtaaagactg gatgtccatc tcacacgaat
     2821 ttatggctat gggcaacaca taatcctagt gcaatatgat actggggtta ttaagatgtg
     2881 tcccaggcag ggaccaagac aggtgaacca tgttgttaca ctctatttgt aacaagggga
     2941 aagagagtgg acgccgacag cagcggactc cactggttgt ctctaacacc cccgaaaatt
     3001 aaacggggct ccacgccaat ggggcccata aacaaagaca agtggccact cttttttttg
     3061 aaattgtgga gtgggggcac gcgtcagccc ccacacgccg ccctgcggtt ttggactgta
     3121 aaataagggt gtaataactt ggctgattgt aaccccgcta accactgcgg tcaaaccact
     3181 tgcccacaaa accactaatg gcaccccggg gaatacctgc ataagtaggt gggcgggcca
     3241 agataggggc gcgattgctg cgatctggag gacaaattac acacacttgc gcctgagcgc
     3301 caagcacagg gttgttggtc ctcatattca cgaggtcgct gagagcacgg tgggctaatg
     3361 ttgccatggg tagcatatac tacccaaata tctggatagc atatgctatc ctaatctata
     3421 tctgggtagc ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata
     3481 tctgggtagt atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata
     3541 tctgggtagc atatgctatc ctaatmkaka tywgggtagt atatgctatc ctaatttata
     3601 tctgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     3661 ggtagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     3721 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     3781 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     3841 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctca tgcatataca
     3901 gtcagcatat gatacccagt agtagagtgg gagtgctatc ctttgcatat gccgccacct
     3961 cccaaggggg cgtgaatttt cgctgcttgt ccttttcctg ctggttgctc ccattcttag
     4021 gtgaatttaa ggaggccagg ctaaagccgt cgcatgtctg attgctcacc aggtaaatgt
     4081 cgctaatgtt ttccaacgcg agaaggtgtt gagcgcggag ctgagtgacg tgacaacatg
     4141 ggtatgccca attgccccat gttgggagga cgaaaatggt gacaagacag atggccagaa
     4201 atacaccaac agcacgcatg atgtctactg gggatttatt ctttagtgcg ggggaataca
     4261 cggcttttaa tacgattgag ggcgtctcct aacaagttac atcactcctg cccttcctca
     4321 ccctcatctc catcacctcc ttcatctccg tcatctccgt catcaccctc cgcggcagcc
     4381 ccttccacca taggtggaaa ccagggaggc aaatctactc catcgtcaaa gctgcacaca
     4441 gtcaccctga tattgcaggt aggagcgggc tttgtcataa caaggtcctt aatcgcatcc
     4501 ttcaaaacct cagcaaatat atgagtttgt aaaaagacca tgaaataaca gacaatggac
     4561 tcccttagcg ggccaggttg tgggccgggt ccaggggcca ttccaaaggg gagacgactc
     4621 aatggtgtaa gacgacattg tggaatagca agggcagttc ctcgccttag gttgtaaagg
     4681 gaggtcttac tacctccata tacgaacaca ccggcgaccc aagttccttc gtcggtagtc
     4741 ctttctacgt gactcctagc caggagagct cttaaacctt ctgcaatgtt ctcaaatttc
     4801 gggttggaac ctccttgacc acgatgcttt ccaaaccacc ctcctttttt gcgcctgcct
     4861 ccatcaccct gaccccgggg tccagtgctt gggccttctc ctgggtcatc tgcggggccc
     4921 tgctctatcg ctcccggggg cacgtcaggc tcaccatctg ggccaccttc ttggtggtat
     4981 tcaaaataat cggcttcccc tacagggtgg aaaaatggcc ttctacctgg agggggcctg
     5041 cgcggtggag acccggatga tgatgactga ctactgggac tcctgggcct cttttctcca
     5101 cgtccacgac ctctccccct ggctctttca cgacttcccc ccctggctct ttcacgtcct
     5161 ctaccccggc ggcctccact acctcctcga ccccggcctc cactacctcc tcgaccccgg
     5221 cctccactgc ctcctcgacc ccggcctcca cctcctgctc ctgcccctcc tgctcctgcc
     5281 cctcctcctg ctcctgcccc tcctgctcct gcccctcccg ctcctgctcc tgctcctgtt
     5341 ccaccgtggg tccctttgca gccaatgcaa cttggacgtt tttggggtct ccggacacca
     5401 tctctatgtc ttggccctga tcctgagccg cccggggctc ctggtcttcc gcctcctcgt
     5461 cctcgtcctc ttccccgtcc tcgtccatgg ttatcacccc ctcttctttg aggtccactg
     5521 ccgccggagc cttctggtcc agatgtgtct cccttctctc ctaggccatt tccaggtcct
     5581 gtacctggcc cctcgtcaga catgattcac actaaaagag atcaatagac atctttatta
     5641 gacgacgctc agtgaataca gggagtgcag actcctgccc cctccaacag cccccccacc
     5701 ctcatcccct tcatggtcgc tgtcagacag atccaggtct gaaaattccc catcctccga
     5761 accatcctcg tcctcatcac caattactcg cagcccggaa aactcccgct gaacatcctc
     5821 aagatttgcg tcctgagcct caagccaggc ctcaaattcc tcgtccccct ttttgctgga
     5881 cggtagggat ggggattctc gggacccctc ctcttcctct tcaaggtcac cagacagaga
     5941 tgctactggg gcaacggaag aaaagctggg tgcggcctgt gaggatcagc ttatcgatga
     6001 taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
     6061 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
     6121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     6181 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     6241 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     6301 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     6361 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     6421 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
     6481 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     6541 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     6601 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     6661 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     6721 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
     6781 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     6841 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     6901 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     6961 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     7021 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     7081 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     7141 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     7201 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7261 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7321 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7381 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7441 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7501 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7561 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7621 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7681 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7741 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7801 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     7861 gcggcctttt tacggttcct ggccttttgc tggccttgaa gctgtccctg atggtcgtca
     7921 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga
     7981 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc
     8041 agcgcgtcgg ccccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata
     8101 tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa
     8161 ttcttggagt ggtgaatccg ttagcgaggt gccgccctgc ttcatccccg tggcccgttg
     8221 ctcgcgtttg ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat
     8281 gacacaaacc ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg
     8341 gcggcgcggt ccgaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga
     8401 gcgaccctgc agcgacccgc ttaacagcgt caacagcgtg ccgcagatcc cggggggcaa
     8461 tgagatatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg
     8521 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg
     8581 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat
     8641 gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca
     8701 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg
     8761 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat
     8821 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa
     8881 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg
     8941 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg
     9001 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg
     9061 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat
     9121 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac
     9181 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc
     9241 cttcttgacg agttcttcta aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     9301 gacggcaata aaaagacaga ataaaacgca cgggtgttgg gtcgtttgtt cataaacgcg
     9361 gggttcggtc ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat
     9421 acgcccgcgt ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc
     9481 tcgcagccaa cgtcggggcg gcaggccctg ccatagccac tggccccgtg ggttagggac
     9541 ggggtccccc atggggaatg gtttatggtt cgtgggggtt attattttgg gcgttgcgtg
     9601 gggtcaggtc cacgactgga ctgagcagac agacccatgg tttttggatg gcctgggcat
     9661 ggaccgcatg tactggcgcg acacgaacac cgggcgtctg tggctgccaa acacccccga
     9721 cccccaaaaa ccaccgcgcg gatttctggc gtgccaagct agtcgaccaa ttctcatgtt
     9781 tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg
     9841 gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg
     9901 ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa
     9961 tatgtacatt tatattggct catgtccaat atgaccgcca tgtt
//