FU-tet-o-hc-myc

LOCUS       FU-tet-o-hc-myc               9895 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    FU-tet-o-hc-myc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9895)
  AUTHORS   Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger 
            K
  TITLE     A high-efficiency system for the generation and study of human 
            induced pluripotent stem cells.
  JOURNAL   Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
  PUBMED    18786420
REFERENCE   2  (bases 1 to 9895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9895
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2610..2880
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2913..2950
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2947..2971
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      3074..3083
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3089..3115
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3134..3160
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3179..3205
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3227..4543
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="PLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPS
                     EDIWKKFELLPAPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTEL
                     LGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPNP
                     ARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDSL
                     LSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGSP
                     SAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNRK
                     CTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKAT
                     AYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    4587..5175
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4640..4660)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5058..5069)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5178..5194)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5179..5195)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             5700..5880
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5906..5923)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5912..6136
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6182..6610
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6269..6288)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6479..6500
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6619..6639)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6624..6953
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6804..6939
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6866..6885
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7001..7048
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7067..7441
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7571..7692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7608..7627)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7662..7681
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7741..7757)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7741..7757)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7754..7776)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7765..7781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7789..7819)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7834..7855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7972..7989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8143..8731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8223..8242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(8902..9762)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9525..9544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9763..9867)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
     2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
     3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattga tcttcggcca
     3061 gcgactagta gaagccacca tggatagcta tccttatgac gtgcctgact atgccagcct
     3121 gatgctgatg agctatcctt atgacgtgcc tgactatgcc agcctgatgc tgatgagcta
     3181 tccttatgac gtgcctgact atgccagcct gatgctgatg tctagacccc tcaacgttag
     3241 cttcaccaac aggaactatg acctcgacta cgactcggtg cagccgtatt tctactgcga
     3301 cgaggaggag aacttctacc agcagcagca gcagagcgag ctgcagcccc cggcgcccag
     3361 cgaggatatc tggaagaaat tcgagctgct gcccgcgccg cccctgtccc ctagccgccg
     3421 ctccgggctc tgctcgccct cctacgttgc ggtcacaccc ttctcccttc ggggagacaa
     3481 cgacggcggt ggcgggagct tctccacggc cgaccagctg gagatggtga ccgagctgct
     3541 gggaggagac atggtgaacc agagtttcat ctgcgacccg gacgacgaga ccttcatcaa
     3601 aaacatcatc atccaggact gtatgtggag cggcttctcg gccgccgcca agctcgtctc
     3661 agagaagctg gcctcctacc aggctgcgcg caaagacagc ggcagcccga accccgcccg
     3721 cggccacagc gtctgctcca cctccagctt gtacctgcag gatctgagcg ccgccgcctc
     3781 agagtgcatc gacccctcgg tggtcttccc ctaccctctc aacgacagca gctcgcccaa
     3841 gtcctgcgcc tcgcaagact ccagcgcctt ctctccgtcc tcggattctc tgctctcctc
     3901 gacggagtcc tccccgcagg gcagccccga gcccctggtg ctccatgagg agacaccgcc
     3961 caccaccagc agcgactctg aggaggaaca agaagatgag gaagaaatcg atgttgtttc
     4021 tgtggaaaag aggcaggctc ctggcaaaag gtcagagtct ggatcacctt ctgctggagg
     4081 ccacagcaaa cctcctcaca gcccactggt cctcaagagg tgccacgtct ccacacatca
     4141 gcacaactac gcagcgcctc cctccactcg gaaggactat cctgctgcca agagggtcaa
     4201 gttggacagt gtcagagtcc tgagacagat cagcaacaac cgaaaatgca ccagccccag
     4261 gtcctcggac accgaggaga atgtcaagag gcgaacacac aacgtcttgg agcgccagag
     4321 gaggaacgag ctaaaacgga gcttttttgc cctgcgtgac cagatcccgg agttggaaaa
     4381 caatgaaaag gcccccaagg tagttatcct taaaaaagcc acagcataca tcctgtccgt
     4441 ccaagcagag gagcaaaagc tcatttctga agaggacttg ttgcggaaac gacgagaaca
     4501 gttgaaacac aaacttgaac agctacggaa ctcttgtgcg taagtcgacg gatcctgaga
     4561 attaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     4621 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     4681 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     4741 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     4801 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     4861 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     4921 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     4981 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     5041 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     5101 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     5161 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     5221 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     5281 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     5341 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5401 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     5461 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     5521 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     5581 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     5641 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     5701 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5761 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5821 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5881 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     5941 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     6001 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     6061 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     6121 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     6181 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     6241 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     6301 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     6361 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     6421 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     6481 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6541 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6601 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6661 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6721 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6781 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6841 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     6901 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     6961 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     7021 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     7081 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     7141 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     7201 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     7261 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     7321 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     7381 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     7441 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     7501 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     7561 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7621 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     7681 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     7741 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     7801 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     7861 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     7921 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     7981 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     8041 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     8101 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     8161 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     8221 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     8281 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     8341 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     8401 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     8461 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     8521 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8581 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8641 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8701 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8761 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8821 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8881 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8941 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     9001 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     9061 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     9121 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     9181 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     9241 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     9301 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     9361 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     9421 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     9481 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9541 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9601 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9661 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9721 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9781 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9841 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//