| 载体名称: | pT2-mVenus |
|---|---|
| 质粒类型: | 哺乳动物细胞载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 6.1 kb |
| 5’测序引物: | CMV-F: CGCAAATGGGCGGTAGGCGTG |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha 等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 产品目录号: | V56896 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
| 备注: | pT2-mVenus适合哺乳细胞表达黄色荧光蛋白mVenus质粒。 |
买家导航
pT2-mVenus载体质粒基本信息
pT2-mVenus质粒图谱载体图谱和pT2-mVenus载体序列质粒序列多克隆位点信息
pT2-mVenus质粒载体简介
pT2-mVenus质粒序列载体序列
LOCUS pT2-mVenus 6123 bp DNA circular SYN 24-AUG-2024
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6123)
AUTHORS Ming
TITLE Direct Submission
JOURNAL Exported Aug 24, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|1066521600|
COMMENT VNTDBDATE|1066521600|
COMMENT VNTNAME|pT2-mVenus|
COMMENT VNTAUTHORNAME|Ming|
FEATURES Location/Qualifiers
enhancer 365..668
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 671..946
/label=chicken beta-actin promoter
intron 947..1955
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
CDS 1976..2695
/codon_start=1
/label=mVenus
polyA_signal 2708..2829
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(3231..3249)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3259..3275)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin complement(3416..3871)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3898..4002
/gene="bla"
/label=AmpR promoter
CDS 4003..4863
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 5034..5622
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5910..5931
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5946..5976
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5984..6000
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6008..6024
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 6045..6063
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
1 tatacagttg aagtcggaag tttacataca ccttagccaa atacatttaa actcactttt
61 tcacaattcc tgacatttaa tcctagtaaa aattccctgt cttaggtcag ttaggatcac
121 cactttattt taagaatgtg aaatatcaga ataatagtag agagaatgat tcatttcagc
181 ttttatttct ttcatcacat tcccagtggg tcagaagttt acatacactc aattagtatt
241 tggtagcatt gcctttaaat tgtttaactt gggtcaaaca tttcgagtag ccttccacaa
301 gctctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
361 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
421 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
481 gtcaatgggt ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
541 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
601 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
661 ttaccatggg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc
721 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg
781 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
841 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
901 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt
961 gccttcgccc cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac
1021 cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg
1081 cttggtttaa tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg
1141 ggagggccct ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg
1201 ggagcgccgc gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg
1261 gctttgtgcg ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggtgc
1321 gggggggctg cgaggggaac aaaggctgcg tgcggggtgt gtgcgtgggg gggtgagcag
1381 ggggtgtggg cgcggcggtc gggctgtaac ccccccctgc acccccctcc ccgagttgct
1441 gagcacggcc cggcttcggg tgcggggctc cgtgcggggc gtggcgcggg gctcgccgtg
1501 ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc tcgggccggg
1561 gagggctcgg gggaggggcg cggcggcccc ggagcgccgg cggctgtcga ggcgcggcga
1621 gccgcagcca ttgcctttta tggtaatcgt gcgagagggc gcagggactt cctttgtccc
1681 aaatctggcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg ggcgcgggcg
1741 aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc
1801 cgccgtcccc ttctccatct ccagcctcgg ggctgccgca gggggacggc tgccttcggg
1861 ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg
1921 ctaaccatgt tcatgccttc ttctttttcc tacagatcct taattaatct agaccatggt
1981 gtccaagggc gaggagctgt tcaccggcgt ggtgcccatc ctggtggagc tggacggcga
2041 cgtgaacggc cacaagttct ccgtgagcgg cgagggcgag ggcgacgcca cctacggcaa
2101 gctgaccctg aagctgatct gcaccaccgg caagctgcct gtgccctggc ctaccctggt
2161 gaccaccctg ggctacggcc tgcagtgctt cgccagatac cccgaccaca tgaagcagca
2221 cgacttcttc aagtccgcca tgcctgaggg ctacgtgcag gagagaacca tcttcttcaa
2281 ggacgacggc aactacaaga ccagagccga ggtgaagttc gagggcgaca ccctggtgaa
2341 cagaatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg gccacaagct
2401 ggagtacaac tacaacagcc acaacgtgta catcaccgcc gacaagcaga agaacggcat
2461 caaggccaac ttcaagatca ggcacaacat cgaggacggc ggcgtgcagc tggccgacca
2521 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
2581 gagctaccag agcgccctga gcaaggaccc taacgagaag agggaccaca tggtgctgct
2641 ggagttcgtg accgccgccg gcatcaccct gggcatggac gagctgtaca agtgagcggc
2701 cgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
2761 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
2821 tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
2881 gttaaatcct cgagtgcgtc cagctagcac gactagagct tctaaagcca tgacatcatt
2941 ttctggaatt ttccaagctg tttaaaggca cagtcaactt agtgtatgta aacttctgac
3001 ccactggaat tgtgatacag tgaattataa gtgaaataat ctgtctgtaa acaattgttg
3061 gaaaaatgac ttgtgtcatg cacaaagtag atgtcctaac tgacttgcca aaactattgt
3121 ttgttaacaa gaaatttgtg gagtagttga aaaacgagtt ttaatgactc caacttaagt
3181 gtatgtaaac ttccgacttc aactgtatag ggatccgagc tccaattcgc cctatagtga
3241 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
3301 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
3361 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaaatt
3421 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
3481 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
3541 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
3601 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
3661 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
3721 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
3781 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
3841 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
3901 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
3961 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
4021 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
4081 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
4141 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
4201 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
4261 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
4321 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
4381 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
4441 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
4501 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
4561 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
4621 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
4681 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
4741 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
4801 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
4861 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
4921 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
4981 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
5041 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
5101 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
5161 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
5221 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
5281 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
5341 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
5401 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
5461 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
5521 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
5581 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
5641 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
5701 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
5761 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
5821 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
5881 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
5941 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
6001 tttcacacag gaaacagcta tgaccatgat tacgccaagc tcgaaattaa ccctcactaa
6061 agggaacaaa agctgggtac cgggcccccc ctcgatcgag gtcgactcta gctagaggat
6121 ccc
//
pT2-mVenus载体图谱质粒图谱pdf版和pT2-mVenus载体序列质粒序列等相关资料下载
pT2-mVenus质粒载体应用举例
- 上一篇：pRK5
- 下一篇：没有了
