| 质粒名称: | pBGK |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 65796 |
| 存储实验室: | Masato Ohtsuka |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体出品公司: | Clontech |
| 空载体大小: | 9968 bp |
| 完整质粒大小: | 9968 bp |
| 载体类型: | Mammalian Expression, CRISPR |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pBGK was a gift from Masato Ohtsuka (Addgene plasmid # 65796 ; http://n2t.net/addgene:65796 ; RRID:Addgene_65796) |
| 参考文献部分: | Mouse Genome Editing Using the CRISPR/Cas System. Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu L, Gurumurthy CB. Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi: 10.1002/0471142905.hg1507s83. 10.1002/0471142905.hg1507s83 PubMed 25271839 |
买家导航
pBGK载体质粒基本信息
pBGK质粒图谱载体图谱和pBGK载体序列质粒序列多克隆位点信息
pBGK质粒载体简介
pBGK质粒序列载体序列
LOCUS pBGK 9968 bp DNA circular SYN 14-APR-2025
DEFINITION Vector for Cas9 mRNA synthesis.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9968)
AUTHORS Harms DW, Quadros RM, Seruggia D, Ohtsuka M, Takahashi G, Montoliu
L, Gurumurthy CB
TITLE Mouse Genome Editing Using the CRISPR/Cas System.
JOURNAL Curr Protoc Hum Genet. 2014 Oct 1;83:15.7.1-15.7.27. doi:
10.1002/0471142905.hg1507s83.
PUBMED 25271839
REFERENCE 2 (bases 1 to 9968)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
Hum Genet."; date: "2014-10-1"; volume: "83:15.7.1-15.7.27. doi";
pages: " 10.1002/0471142905.hg1507s83"
COMMENT Sequence Label: pBGK
FEATURES Location/Qualifiers
source 1..9968
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 769..789
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 863..882
/label=T7
/note="T7 promoter, forward primer"
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 956..965
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 965..1030
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 1037..1057
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 1082..5182
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 1082..5182
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 5183..5230
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 5471..5500
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 5516..5533
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5556..5573)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5562..5786
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5832..6260
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5919..5938)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6129..6150
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6269..6289)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6274..6604
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6455..6590
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6517..6536
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6671..7465
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6725..6744)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7335..7354
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7641..7762
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7678..7697)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7732..7751
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7811..7827)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7811..7827)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7824..7846)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7835..7851
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(join(7859..7865,7866..7883,7884..7889))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7904..7925
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(8042..8059)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8213..8801)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8293..8312)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(8972..9763,9764..9832))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9595..9614
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9833..9937)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt
901 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattgca ccggtgccac
961 catggactat aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga
1021 tgacgataag atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc
1081 cgacaagaag tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat
1141 caccgacgag tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca
1201 cagcatcaag aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc
1261 cacccggctg aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta
1321 tctgcaagag atcttcagca acgagatggc caaggtggac gacagcttct tccacagact
1381 ggaagagtcc ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa
1441 catcgtggac gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa
1501 actggtggac agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat
1561 gatcaagttc cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt
1621 ggacaagctg ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat
1681 caacgccagc ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg
1741 gctggaaaat ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct
1801 gattgccctg agcctgggcc tgacccccaa cttcaagagc aacttcgacc tggccgagga
1861 tgccaaactg cagctgagca aggacaccta cgacgacgac ctggacaacc tgctggccca
1921 gatcggcgac cagtacgccg acctgtttct ggccgccaag aacctgtccg acgccatcct
1981 gctgagcgac atcctgagag tgaacaccga gatcaccaag gcccccctga gcgcctctat
2041 gatcaagaga tacgacgagc accaccagga cctgaccctg ctgaaagctc tcgtgcggca
2101 gcagctgcct gagaagtaca aagagatttt cttcgaccag agcaagaacg gctacgccgg
2161 ctacattgac ggcggagcca gccaggaaga gttctacaag ttcatcaagc ccatcctgga
2221 aaagatggac ggcaccgagg aactgctcgt gaagctgaac agagaggacc tgctgcggaa
2281 gcagcggacc ttcgacaacg gcagcatccc ccaccagatc cacctgggag agctgcacgc
2341 cattctgcgg cggcaggaag atttttaccc attcctgaag gacaaccggg aaaagatcga
2401 gaagatcctg accttccgca tcccctacta cgtgggccct ctggccaggg gaaacagcag
2461 attcgcctgg atgaccagaa agagcgagga aaccatcacc ccctggaact tcgaggaagt
2521 ggtggacaag ggcgcttccg cccagagctt catcgagcgg atgaccaact tcgataagaa
2581 cctgcccaac gagaaggtgc tgcccaagca cagcctgctg tacgagtact tcaccgtgta
2641 taacgagctg accaaagtga aatacgtgac cgagggaatg agaaagcccg ccttcctgag
2701 cggcgagcag aaaaaggcca tcgtggacct gctgttcaag accaaccgga aagtgaccgt
2761 gaagcagctg aaagaggact acttcaagaa aatcgagtgc ttcgactccg tggaaatctc
2821 cggcgtggaa gatcggttca acgcctccct gggcacatac cacgatctgc tgaaaattat
2881 caaggacaag gacttcctgg acaatgagga aaacgaggac attctggaag atatcgtgct
2941 gaccctgaca ctgtttgagg acagagagat gatcgaggaa cggctgaaaa cctatgccca
3001 cctgttcgac gacaaagtga tgaagcagct gaagcggcgg agatacaccg gctggggcag
3061 gctgagccgg aagctgatca acggcatccg ggacaagcag tccggcaaga caatcctgga
3121 tttcctgaag tccgacggct tcgccaacag aaacttcatg cagctgatcc acgacgacag
3181 cctgaccttt aaagaggaca tccagaaagc ccaggtgtcc ggccagggcg atagcctgca
3241 cgagcacatt gccaatctgg ccggcagccc cgccattaag aagggcatcc tgcagacagt
3301 gaaggtggtg gacgagctcg tgaaagtgat gggccggcac aagcccgaga acatcgtgat
3361 cgaaatggcc agagagaacc agaccaccca gaagggacag aagaacagcc gcgagagaat
3421 gaagcggatc gaagagggca tcaaagagct gggcagccag atcctgaaag aacaccccgt
3481 ggaaaacacc cagctgcaga acgagaagct gtacctgtac tacctgcaga atgggcggga
3541 tatgtacgtg gaccaggaac tggacatcaa ccggctgtcc gactacgatg tggaccatat
3601 cgtgcctcag agctttctga aggacgactc catcgacaac aaggtgctga ccagaagcga
3661 caagaaccgg ggcaagagcg acaacgtgcc ctccgaagag gtcgtgaaga agatgaagaa
3721 ctactggcgg cagctgctga acgccaagct gattacccag agaaagttcg acaatctgac
3781 caaggccgag agaggcggcc tgagcgaact ggataaggcc ggcttcatca agagacagct
3841 ggtggaaacc cggcagatca caaagcacgt ggcacagatc ctggactccc ggatgaacac
3901 taagtacgac gagaatgaca agctgatccg ggaagtgaaa gtgatcaccc tgaagtccaa
3961 gctggtgtcc gatttccgga aggatttcca gttttacaaa gtgcgcgaga tcaacaacta
4021 ccaccacgcc cacgacgcct acctgaacgc cgtcgtggga accgccctga tcaaaaagta
4081 ccctaagctg gaaagcgagt tcgtgtacgg cgactacaag gtgtacgacg tgcggaagat
4141 gatcgccaag agcgagcagg aaatcggcaa ggctaccgcc aagtacttct tctacagcaa
4201 catcatgaac tttttcaaga ccgagattac cctggccaac ggcgagatcc ggaagcggcc
4261 tctgatcgag acaaacggcg aaaccgggga gatcgtgtgg gataagggcc gggattttgc
4321 caccgtgcgg aaagtgctga gcatgcccca agtgaatatc gtgaaaaaga ccgaggtgca
4381 gacaggcggc ttcagcaaag agtctatcct gcccaagagg aacagcgata agctgatcgc
4441 cagaaagaag gactgggacc ctaagaagta cggcggcttc gacagcccca ccgtggccta
4501 ttctgtgctg gtggtggcca aagtggaaaa gggcaagtcc aagaaactga agagtgtgaa
4561 agagctgctg gggatcacca tcatggaaag aagcagcttc gagaagaatc ccatcgactt
4621 tctggaagcc aagggctaca aagaagtgaa aaaggacctg atcatcaagc tgcctaagta
4681 ctccctgttc gagctggaaa acggccggaa gagaatgctg gcctctgccg gcgaactgca
4741 gaagggaaac gaactggccc tgccctccaa atatgtgaac ttcctgtacc tggccagcca
4801 ctatgagaag ctgaagggct cccccgagga taatgagcag aaacagctgt ttgtggaaca
4861 gcacaagcac tacctggacg agatcatcga gcagatcagc gagttctcca agagagtgat
4921 cctggccgac gctaatctgg acaaagtgct gtccgcctac aacaagcacc gggataagcc
4981 catcagagag caggccgaga atatcatcca cctgtttacc ctgaccaatc tgggagcccc
5041 tgccgccttc aagtactttg acaccaccat cgaccggaag aggtacacca gcaccaaaga
5101 ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga cacggatcga
5161 cctgtctcag ctgggaggcg acaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa
5221 aaagaaaaag taagaattct gcagatatcc agcacagtgg cggccgctcg actatgggca
5281 ccaaagaacc tgtaaacgtt atctttttaa attgaatgtg cacaaataaa agtttggaaa
5341 agaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
5401 aaaaaaaaaa aaaaaaaaaa aaaaatacgc atcatacgct gtggcgtacc tcgagtctag
5461 agggcccttc gaacaaaaac tcatctcaga agaggatctg aatatgcata ccggtcatca
5521 tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
5581 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
5641 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
5701 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
5761 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
5821 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
5881 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
5941 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcggggca tccctttagg
6001 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
6061 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
6121 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
6181 ttttgattta taagggattt tggggatttc ggcctattgg ttaaaaaatg agctgattta
6241 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
6301 ccaggctccc caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag
6361 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
6421 gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
6481 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
6541 ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
6601 caaaaagctc ccgggagctt gtatatccat tttcggatct gatcaagaga caggatgagg
6661 atcgtttcgc atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga
6721 gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt
6781 ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct
6841 gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg
6901 cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt
6961 gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc
7021 tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc
7081 gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga
7141 tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg
7201 catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat
7261 ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg
7321 ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc
7381 tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta
7441 tcgccttctt gacgagttct tctgagcggg actctggggt tcgcgaaatg accgaccaag
7501 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
7561 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
7621 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
7681 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
7741 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
7801 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
7861 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
7921 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
7981 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
8041 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
8101 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
8161 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
8221 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
8281 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
8341 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
8401 tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
8461 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
8521 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
8581 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
8641 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
8701 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
8761 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
8821 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
8881 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
8941 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
9001 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
9061 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
9121 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
9181 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
9241 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
9301 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
9361 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
9421 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
9481 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
9541 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
9601 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
9661 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
9721 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
9781 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
9841 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
9901 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
9961 ctgacgtc
//
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