pYSJ16

LOCUS       pYSJ16       7058 bp DNA     circular SYN 12-OCT-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 5, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7058
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     914..950
                     /label=oYSJ047-ERF-F
     misc_feature    934..2582
                     /label=ERF
     primer_bind     complement(2561..2602)
                     /label=oYSJ048-ERF-R
     polyA_signal    2658..2882
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2928..3356
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        3370..3699
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3550..3685
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             3766..4560
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4734..4855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4904..4920)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4928..4944
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4952..4982)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4997..5018
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(5306..5891)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6062..6922)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6923..7027)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaactt aagcttggta ccgagctcgg atccatgaag accccggcgg acacagggtt
      961 tgccttcccg gattgggcct acaagccaga gtcgtcccct ggctcaaggc agatccagct
     1021 gtggcacttt atcctggagc tgctgcggaa ggaggagtac cagggcgtca ttgcctggca
     1081 gggggactac ggggaattcg tcatcaaaga ccctgatgag gtggcccggc tgtggggcgt
     1141 tcgcaagtgc aagccccaga tgaattacga caagctgagc cgggccctgc gctattacta
     1201 taacaagcgc attctgcaca agaccaaggg gaaacggttc acctacaagt tcaatttcaa
     1261 caaactggtg ctggtcaatt acccattcat tgatgtgggg ttggctgggg gtgcagtgcc
     1321 ccagagtgcc ccgccagtgc cgtcgggtgg tagccacttc cgcttccctc cctcaacgcc
     1381 ctccgaggtg ctgtccccca ccgaggaccc ccgctcacca ccagcctgct cttcatcttc
     1441 atcttccctc ttctcggctg tggtggcccg ccgcctgggc cgaggctcag tcagtgactg
     1501 tagtgatggc acgtcagagc tggaggaacc gctgggagag gatccccgcg cccgaccacc
     1561 cggccctccg gatctgggtg ccttccgagg gcccccgctg gcccgcctgc cccatgaccc
     1621 tggtgtcttc cgagtctatc cccggcctcg gggtggccct gaacccctca gccccttccc
     1681 tgtgtcgcct ctggccggtc ctggatccct gctgccccct cagctctccc cggctctgcc
     1741 catgacgccc acccacctgg cctacactcc ctcgcccacg ctgagcccga tgtaccccag
     1801 tggtggcggg gggcccagcg gctcaggggg aggctcccac ttctccttca gccctgagga
     1861 catgaaacgg tacctgcagg cccacaccca aagcgtctac aactaccacc tcagcccccg
     1921 cgccttcctg cactaccctg ggctggtggt gccccagccc cagcgccctg acaagtgccc
     1981 gctgccgccc atggcacccg agaccccacc ggtcccctcc tcggcctcgt catcctcttc
     2041 ttcttcttcc tccccattca agtttaagct ccagccgccc ccactcggac gccggcagcg
     2101 ggcagctggg gagaaggccg tagccggtgc tgacaagagc ggtggcagtg caggcgggct
     2161 ggctgagggg gcaggggcgc tagccccacc gcccccgcca ccacagatca aggtggagcc
     2221 catctcggaa ggcgagtcgg aggaggtaga ggtgactgac atcagtgatg aggatgagga
     2281 agacggggag gtgttcaaga cgccccgtgc cccacctgca ccccctaagc ctgagcccgg
     2341 cgaggcaccc ggggcatccc agtgcatgcc cctcaagcta cgctttaagc ggcgctggag
     2401 tgaagactgt cgcctcgaag ggggtggggg ccccgctggg ggctttgagg atgagggtga
     2461 ggacaagaag gtgcgtgggg aggggcctgg ggaggctggg gggcccctca ccccaaggcg
     2521 ggtgagctct gacctccagc atgccacggc ccagctctcc ctggagcacc gagactcctg
     2581 agaattctgc agatatccag cacagtggcg gccgctcgag tctagagggc ccgtttaaac
     2641 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2701 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2761 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2821 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     2881 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
     2941 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3001 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3061 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3121 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3181 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3241 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3301 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
     3361 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     3421 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     3481 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     3541 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     3601 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
     3661 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
     3721 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     3781 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     3841 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     3901 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     3961 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     4021 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     4081 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     4141 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     4201 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     4261 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     4321 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     4381 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     4441 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     4501 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     4561 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     4621 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     4681 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt
     4741 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     4801 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     4861 tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg
     4921 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4981 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     5041 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     5101 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5161 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5221 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5821 aacaaaccac cgctggtagc ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6361 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6661 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7021 ttccgcgcac atttccccga aaagtgccac ctgacgtc
//