| 质粒名称: | pAAVLINKv1-pCALM1-Cre-LAMA1-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001745 |
| 产品用途: | 3' AAVLINK plasmid for LAMA1 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | LAMA1 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-LAMA1-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-LAMA1-C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-LAMA1-C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-LAMA1-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-LAMA1-C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-LAMA1-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-LAMA1-C-FLAG 7135 bp DNA circular SYN 22-JUL-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7135)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7135
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..141
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..141
/label=AAV2 ITR
promoter 156..276
/label=pCALM1
CDS 288..1319
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1320..1360
/label=Splicing Donor
protein_bind 1367..1400
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1401..1492
/label=Splicing Acceptor
/note="Splicing acceptor"
misc_feature 1493..3848
/label=LAMA1
misc_feature 3137..3142
/label=SDS
misc_feature 3388..3393
/label=SDS
misc_feature 3407..3412
/label=SDS
CDS 3855..3920
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3924..4355
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 4068..4073
/label=SDS
repeat_region 4398..4538
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4398..4527
/label=AAV2 ITR
rep_origin 4613..5068
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5350..5454
/gene="bla"
/label=AmpR promoter
CDS 5455..6315
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6486..7074
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
121 actccatcac taggggttcc tgcggccgca cgcgtcgctc tgcgcgaggc ggctccgccg
181 cggcggaggg atacggcgca ccatatatat atcgcggggc gcagactcgc gctccggcag
241 tggtgctggg agtgtcgtgg acgccgtgcc gttactcgta cgccaccatg tccaatttac
301 tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat gaggttcgca
361 agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc tggaaaatgc
421 ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg aaatggtttc
481 ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc ggtctggcag
541 taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg tccgggctgc
601 cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga aaagaaaacg
661 ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat ttcgaccagg
721 ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg gcatttctgg
781 ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg gttaaagata
841 tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa acgctggtta
901 gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc gagcgatgga
961 tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg gtcagaaaaa
1021 atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg gaagggattt
1081 ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag agatacctgg
1141 cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc gctggagttt
1201 caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgta atgaactata
1261 tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat ggcgattagg
1321 taagtatcaa ggttacaaga caggtttaag gagaccaata actagtataa cttcgtatag
1381 gatactttat agcaatttat gaaactgggc ttgtcgagac agagaagact cttgcgtttc
1441 tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac agggaaggca
1501 agtgccgtgg gtgcttcgga agctcccaga atgaagaccc ttccttccat tttgacggga
1561 gtgggtactc tgtcgtggag aagtcacttc cggctaccgt gacccagata atcatgcttt
1621 ttaatacctt ttcacctaat ggacttcttc tctacctggg ttcatacggc acaaaagact
1681 ttttatccat cgagctgttt cgtggcagag tgaaggttat gactgacctg ggttcaggac
1741 ccattaccct tttgacagac agacgttata acaatggaac ctggtacaaa attgccttcc
1801 agcgaaaccg gaagcaagga gtgctagcag ttatcgatgc ctataacacc agtaataaag
1861 aaaccaagca gggcgagact ccgggagcat cttctgacct caaccgccta gacaaggacc
1921 cgatttatgt gggtggatta ccaaggtcaa gagttgtaag gagaggtgtc accaccaaaa
1981 gctttgtggg ctgcatcaag aacctggaaa tatccagatc aacctttgac ttactcagaa
2041 attcctatgg agtgagaaaa ggctgtttac tggagcccat ccggagtgtt agcttcctga
2101 aaggcggcta cattgaattg ccacccaaat ctttgtcacc agaatcagaa tggctggtaa
2161 catttgccac cacgaacagc agtggcatca tcctggctgc cctcggcggg gatgtggaga
2221 agcggggtga tcgtgaggaa gcacacgtgc ccttcttttc cgtcatgctg atcggaggca
2281 acattgaggt acatgtcaat cctggggatg ggacaggcct gagaaaagct ctcctgcacg
2341 ctcccacggg tacctgcagt gatggacaag cgcattccat ctccttggtc aggaatcgga
2401 gaattatcac tgtccaattg gatgagaaca atcctgtgga aatgaagttg ggcacattag
2461 tagaaagcag gacgataaat gtgtccaatc tgtacgtcgg gggaattcca gagggagagg
2521 ggacgtcact gctcacaatg agaagatcgt tccatggctg tatcaaaaac ctgatcttca
2581 atttggaact tttggatttc aacagtgcag ttggccatga gcaagtcgac ctggacacct
2641 gctggctgtc agaaaggcct aagctggctc ccgatgcaga ggacagcaag ctcttgccag
2701 agccccgggc ttttccagaa cagtgtgtgg tggatgcagc tctggagtac gttcccggcg
2761 ctcaccagtt tggtctcaca caaaacagcc atttcatctt gccttttaat cagtcggctg
2821 tcagaaagaa gctctcggtt gagctaagca tccgcacgtt cgcctccagc ggcctgattt
2881 actacatggc tcatcagaac caagcagact acgctgtgct ccagctgcac gggggccgcc
2941 tccacttcat gtttgacctt ggcaaaggca gaacaaaggt ctctcaccct gcactgctca
3001 gtgatggcaa gtggcacacg gtcaagacag actatgttaa aagaaaaggc ttcataactg
3061 tcgacggccg agagtctccc atggtgactg tggtgggaga tggaaccatg ctggatgtgg
3121 agggtttgtt ctacctagga ggcctgccct cccagtacca ggccaggaaa attggaaata
3181 tcacccacag catccctgcc tgcattgggg atgtgacggt taacagcaaa cagctggaca
3241 aggacagccc ggtgtctgcc ttcacggtga acaggtgcta cgcagtggcc caggaaggaa
3301 catactttga cggaagcgga tatgcagctc ttgtcaaaga gggctacaaa gtccagtcag
3361 atgtgaacat cacactggag tttcgaacct cctcgcagaa tggcgtcctc ctggggatca
3421 gcactgccaa agtggatgcc attggactag agcttgtgga cggcaaggtc ttgttccatg
3481 tcaacaatgg tgctggcagg ataacagctg catatgagcc caaaaccgcc actgtgctct
3541 gtgatggaaa atggcacact cttcaagcta acaaaagcaa acaccgtatc actctgattg
3601 ttgacgggaa cgcagttggc gctgaaagtc cacacaccca gtctacctca gtggacacca
3661 acaatcccat ttatgttggt ggctatcctg ctggtgtgaa gcaaaaatgc ctgcgcagcc
3721 agacctcgtt ccgcgggtgt ttgaggaagc tagctctgat taagagcccg caggtgcagt
3781 cctttgactt cagcagagcg ttcgaactgc acggagtttt ccttcattcc tgtcctggga
3841 ccgagtcctg tacagactac aaagaccatg acggtgatta taaagatcat gacatcgact
3901 acaaggatga cgatgacaag taactcgaga taatcaacct ctggattaca aaatttgtga
3961 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
4021 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
4081 atcctggtta gttcttgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
4141 aggggctcgg ctgttgggca ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc
4201 tattgcttta tttgtaacca tctagcttta tttgtgaaat ttgtgatgct attgctttat
4261 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
4321 ttcaggttca gggggagatg tgggaggttt tttaaagatc tggagaccgg tgtggcacgt
4381 gcggaccgag cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc
4441 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
4501 ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct
4561 ccttacgcat ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc
4621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
4681 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
4741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
4801 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
4861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
4921 ttccaaactg gaacaacact caaccctatc tcgggctatt cttttgattt ataagggatt
4981 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
5041 tttaacaaaa tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat
5101 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
5161 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
5221 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
5281 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
5341 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
5401 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
5461 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
5521 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
5581 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
5641 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
5701 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
5761 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
5821 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
5881 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
5941 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
6001 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
6061 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
6121 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
6181 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
6241 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
6301 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
6361 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
6421 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
6481 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
6541 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
6601 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
6661 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
6721 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
6781 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
6841 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
6901 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
6961 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
7021 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
7081 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//
