pBAD-HisB-Pwopol

LOCUS       pBAD-HisB-Pwopol                6291 bp DNA     circular SYN 17-JUN-2026
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    pBAD-HisB-mBaoJin2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Replikant
  TITLE     Direct Submission
  JOURNAL   Exported Sep 26, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6291
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..285
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     misc_feature    317..2652
                     /label=-3
     terminator      2862..2948
                     /gene="Escherichia coli rrnB"
                     /label=Escherichia coli rrnB terminator
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3040..3067
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3086..3177
                     /gene="bla"
                     /label=bla promoter
                     /note="AmpR promoter"
     CDS             3178..4038
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4209..4797
                     /direction=RIGHT
                     /label=ori
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    4983..5123
                     /label=bom
                     /note="bom"
                     /note="basis of mobility region from pBR322"
     CDS             complement(5387..6265)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /note="araC"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
       61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
      121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
      181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
      241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
      301 taacaggagg aattaaccat gatcctggat gtggactaca tcaccgagga aggtaagccg
      361 gtgatccgtc tgttcaaaaa agaaaacggt aagttcaaaa ttgagcacga ccgtaccttc
      421 cgtccatata tctacgcgct gctccgcgac gattccaaaa ttgaagaagt caaaaagatc
      481 accggtgaac gtcatgggaa aatcgtccgc atcgtggatg tagagaaagt tgaaaaaaag
      541 ttcctgggca aaccgattac cgtttggaaa ctgtatctgg aacacccgca ggatgttcct
      601 acgatccggg aaaaagtgcg cgaacacccg gcggtggttg acatcttcga atacgacatt
      661 ccgttcgcta aacgctacct gatcgataag ggcttgatcc cgatggaggg tgaagaggag
      721 ctcaaaatcc tggcgtttga catcgaaacc ctgtaccatg aaggtgagga gttcggtaaa
      781 ggcccgatca tcatgatctc ctacgcagac gaaaacgaag ctaaggttat cacatggaaa
      841 aacattgatc tgccgtatgt agaagtggtt tcttccgagc gtgagatgat caaacgtttc
      901 ctccgcatta tccgtgaaaa agatcctgat atcatcgtta cctataatgg cgattccttc
      961 gacttcccat acctggctaa acgtgccgaa aaactgggta ttaagctgac catcggccgt
     1021 gacggtagcg agccaaaaat gcagcgaatc ggcgacatga ccgcagtgga ggtgaaaggt
     1081 cgtatccact tcgacctgta ccatgttatc acccgcacca tcaacctgcc gacttatacc
     1141 ctggaggcag tatacgaagc aatcttcggg aagccgaaag aaaaggttta tgcggatgaa
     1201 atcgccaagg cgtgggaatc cggtgaaaac ctggaacgtg ttgcgaaata ttccatggaa
     1261 gatgcgaaag cgacctatga actgggtaaa gagttcctgc cgatggagat tcaactgagc
     1321 cgcctggttg gtcagccgct gtgggatgta tcccgctcca gcaccggcaa tctggttgaa
     1381 tggtttctgc ttcgtaaagc ttatgagcgt aatgaagtgg ctccgaacaa accgagcgaa
     1441 gaagaatatc agcgtcgcct gcgtgaatcc tacactggcg gtttcgtgaa agaaccagag
     1501 aaaggtctgt gggaaaacat cgtatacctg gatttccgcg ccctgtaccc gtcgattatc
     1561 attacccata atgtgtcccc ggatactctt aacctggaag gttgtaagaa ctacgacatt
     1621 gcgccgcagg taggtcataa gttctgcaaa gatatcccag gcttcattcc aagcttgctg
     1681 ggccacctgc tggaagaacg tcaaaaaatt aaaaccaaga tgaaagaaac ccaggaccca
     1741 atcgaaaaaa tcctgctgga ctaccgccag aaagcgatca aacttctggc taactccttc
     1801 tacggttact acggttacgc aaaggcgcgt tggtactgta aagaatgtgc tgaatctgtg
     1861 acagcttggg gccgtaaata catcgaactg gtctggaaag agttggagga gaagttcggc
     1921 ttcaaagttc tctacatcga tacggacggt ctgtatgcaa ccattccggg cggcgaatct
     1981 gaagagatca aaaagaaagc cctggagttt gtcaaataca tcaacagcaa actgccgggc
     2041 ctgctggaac tggagtatga aggcttctac aaacgtggtt tcttcgttac taaaaagcgc
     2101 tacgccgtga tcgatgaaga aggtaaagtg attacccgtg gtctggaaat tgtgcgacgt
     2161 gattggtctg aaattgcgaa ggaaactcag gctcgtgttc tggaaaccat cctcaagcat
     2221 ggtgacgtgg aagaggcagt gcgcatcgtg aaggaagtaa ttcagaagct cgcaaactat
     2281 gaaatcccgc cggagaagct cgctatctac gaacaaatca ctcgcccgct gcacgaatat
     2341 aaagcaatcg ggccgcacgt ggcggtggcc aaaaaattag ctgcgaaggg cgttaaaatc
     2401 aaaccgggca tggtaatcgg ctacattgtt ctgcgtggtg atggtccgat ctctaatcgt
     2461 gcaattctgg cagaagaata cgatccgaaa aagcacaagt acgacgcaga atattatatt
     2521 gaaaaccagg tgctgccggc tgtactgcgt attctggaag gtttcggcta ccgcaaagag
     2581 gacctgcgtt atcagaaaac gcgtcaggtt ggcctgacct cttggctgaa cattaaaaaa
     2641 tcctgagaat tcgaagcttg gctgttttgg cggatgagag aagattttca gcctgataca
     2701 gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg gcagtagcgc
     2761 ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg ccgatggtag
     2821 tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa cgaaaggctc
     2881 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctcctgagta
     2941 ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga gggtggcggg
     3001 caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc ctgacggatg
     3061 gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc aaatatgtat
     3121 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3181 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3241 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3301 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3361 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3421 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3481 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3541 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3601 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3661 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3721 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3781 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3841 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3901 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3961 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4021 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4081 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4141 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4201 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4261 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4321 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4381 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4441 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4501 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4561 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4621 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4681 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4741 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4801 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     4861 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     4921 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     4981 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     5041 actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc
     5101 tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac
     5161 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5221 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg
     5281 cgaaggcgaa gcggcatgca taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa
     5341 ccctatgcta ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga
     5401 cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg ctggccccgg
     5461 tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga
     5521 cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg atacgttggt
     5581 cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg
     5641 acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt
     5701 gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact
     5761 cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct
     5821 ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat
     5881 gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt gacggccagt
     5941 taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc
     6001 gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac
     6061 ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga
     6121 gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt
     6181 tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat cccggcagca
     6241 ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga g
//