| 质粒名称: | pBAD-HisB-T4gene43 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002729 |
| 产品用途: | Bacterial expression of T4 DNA polymerase (gene 43); polymerase that replicates viral genomic DNA with 3' to 5' exonuclease proofreading activity |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pBAD-HisB |
| 表达系统: | 大肠杆菌表达 |
| 启动子: | araBAD |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | T4gene43 |
| 物种来源: | Enterobacteria phage T4 |
| 密码子优化: | 大肠杆菌表达优化 |
pBAD-HisB-T4gene43
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pBAD-HisB-T4gene43载体质粒基本信息
pBAD-HisB-T4gene43质粒图谱载体图谱和pBAD-HisB-T4gene43载体序列质粒序列多克隆位点信息
pBAD-HisB-T4gene43质粒载体简介
pBAD-HisB-T4gene43质粒序列载体序列
LOCUS pBAD-HisB-T4gene43 6660 bp DNA circular SYN 17-JUN-2026
DEFINITION synthetic circular DNA.
ACCESSION .
VERSION .
KEYWORDS pBAD-HisB-mBaoJin2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6660)
AUTHORS Replikant
TITLE Direct Submission
JOURNAL Exported Sep 26, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6660
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..285
/gene="araBAD"
/label=araBAD promoter
/note="araBAD promoter"
/note="promoter of the L-arabinose operon of E. coli; the
araC regulatory gene is transcribed in the opposite
direction (Guzman et al., 1995)"
misc_feature 317..3021
/label=-4
terminator 3231..3317
/gene="Escherichia coli rrnB"
/label=Escherichia coli rrnB terminator
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3409..3436
/note="rrnB T2 terminator"
/note="transcription terminator T2 from the E. coli rrnB
gene"
promoter 3455..3546
/gene="bla"
/label=bla promoter
/note="AmpR promoter"
CDS 3547..4407
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=bla
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4578..5166
/direction=RIGHT
/label=ori
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 5352..5492
/label=bom
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(5756..6634)
/codon_start=1
/gene="araC"
/product="L-arabinose regulatory protein"
/label=araC
/note="araC"
/translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
EKVNDVAVKLS"
ORIGIN
1 aagaaaccaa ttgtccatat tgcatcagac attgccgtca ctgcgtcttt tactggctct
61 tctcgctaac caaaccggta accccgctta ttaaaagcat tctgtaacaa agcgggacca
121 aagccatgac aaaaacgcgt aacaaaagtg tctataatca cggcagaaaa gtccacattg
181 attatttgca cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg
241 atcctacctg acgcttttta tcgcaactct ctactgtttc tccatacccg ttttttgggc
301 taacaggagg aattaaccat gaaagagttc tacatctcca tcgaaaccgt tggtaacaat
361 atcgttgaac gctacattga tgaaaacggt aaagaacgta cccgcgaggt agaatacctc
421 ccgaccatgt tccgtcactg caaagaggaa tctaaataca aagatattta cggtaagaac
481 tgcgctccgc agaagttccc gtccatgaaa gacgcacgtg actggatgaa acgtatggag
541 gacatcggcc tcgaagctct gggcatgaac gacttcaaac tcgcgtacat ctcggacacc
601 tacggctccg aaatcgtgta cgatcgtaag ttcgtgcgcg tagcgaattg cgatatcgaa
661 gtcaccggtg acaagttccc agatccgatg aaagcagaat acgaaattga tgcaatcacc
721 cattatgata gcatcgatga ccgtttctac gtattcgatc tgctgaactc catgtatggt
781 tctgtttcca agtgggacgc taagcttgcc gccaaactgg attgtgaagg tggtgacgag
841 gtgccacagg aaatcctgga tcgtgttatc tatatgccgt tcgataacga acgtgacatg
901 ctgatggaat acattaacct ctgggaacag aaacgcccgg ctattttcac cggttggaac
961 atcgaaggtt tcgatgttcc gtacatcatg aatcgcgtga aaatgattct gggtgagcgt
1021 agcatgaagc gcttctctcc gattggtcgt gttaaatcga aactgatcca gaacatgtac
1081 ggctctaaag aaatctactc gatcgatggc gtgtctattc tggactacct ggatctttac
1141 aagaagttcg cattcaccaa cctgccgtcc ttctctctgg aatccgtagc acagcacgaa
1201 accaaaaaag gcaagctgcc gtatgacggc ccaatcaaca aactgcgtga aactaatcac
1261 cagcgttaca tttcgtacaa cattattgac gttgaatctg tacaggctat cgataaaatc
1321 cgtggcttca tcgatctggt tctgagcatg tcgtactacg ctaaaatgcc gttcagcggt
1381 gttatgtctc cgatcaagac ctgggatgcc atcatcttta actccctgaa aggcgaacac
1441 aaagttattc cgcagcaggg ttcccacgtt aaacagtctt tcccgggcgc atttgttttc
1501 gaacctaaac cgatcgcgcg ccgttacatc atgtcctttg acctgaccag cctgtaccct
1561 agcatcatcc gtcaagtgaa catcagccca gaaaccattc gcggtcaatt caaagtccat
1621 ccgatccacg aatatatcgc tggtaccgcg ccaaaaccga gcgacgaata ctcatgctcg
1681 ccgaacggtt ggatgtatga taaacatcag gagggcatca ttccgaaaga aatcgccaaa
1741 gtgttctttc aacgtaaaga ctggaaaaag aaaatgttcg cggaagaaat gaacgcggaa
1801 gcgatcaaaa aaatcatcat gaagggtgct ggctcttgct cgaccaagcc ggaagtggaa
1861 cgttacgtga agtttagtga tgattttctg aacgagctgt ccaactacac tgaatccgtc
1921 ctgaactcac tgatcgagga gtgtgaaaaa gcagcgacgc tggcgaacac caaccagctg
1981 aaccgtaaaa tcctgatcaa cagcctatac ggtgcactgg gtaacatcca cttccgttac
2041 tacgatctgc gcaacgccac tgcgattacc atcttcgggc aggtgggaat ccagtggatc
2101 gcccgtaaaa ttaacgaata cctgaacaaa gtttgtggta ccaacgacga ggatttcatt
2161 gcagctggcg acacggattc cgtatatgtc tgcgtagaca aggtgattga gaaagtgggt
2221 ctggaccgtt ttaaagaaca gaacgacctg gtcgagttca tgaatcagtt tggcaaaaag
2281 aaaatggaac cgatgatcga tgtcgcatac cgcgaactgt gtgattacat gaacaaccgc
2341 gaacatctga tgcacatgga ccgtgaagcc atatcttgtc ctccactggg ctccaaaggt
2401 gtcggtggct tctggaaggc gaaaaaacgt tatgccctga acgtctatga tatggaggac
2461 aaacgcttcg cggagcctca cctgaaaatt atgggcatgg aaactcagca aagcagcact
2521 ccgaaagcag ttcaagaggc tctggaagaa agcattcgcc gcattctgca agagggtgaa
2581 gaatcggttc aggaatacta caaaaacttc gaaaaagagt accgccaact ggattacaaa
2641 gttatcgccg aggttaaaac cgctaacgat atcgctaagt acgatgataa aggttggccg
2701 ggctttaaat gcccttttca catccgtggt gttctgacat accgtcgtgc tgtttctggt
2761 ctgggcgtag ctccgattct ggacggtaac aaagttatgg tacttccgct gcgtgaaggt
2821 aacccgttcg gtgataaatg tatcgcgtgg ccgtctggta ccgaactgcc gaaggaaatc
2881 cgttcggatg tactgtcttg gattgatcac tctaccctgt tccaaaaatc cttcgttaaa
2941 ccgctggccg gcatgtgcga atccgcagga atggactacg aagaaaaggc ctccttagac
3001 tttctcttcg gctgagaatt cgaagcttgg ctgttttggc ggatgagaga agattttcag
3061 cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg
3121 cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc
3181 cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac
3241 gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
3301 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
3361 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
3421 tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca
3481 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
3541 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
3601 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
3661 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
3721 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
3781 ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
3841 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
3901 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
3961 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
4021 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
4081 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
4141 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
4201 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
4261 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
4321 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
4381 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
4441 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
4501 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
4561 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
4621 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
4681 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
4741 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
4801 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
4861 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
4921 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
4981 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
5041 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
5101 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
5161 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
5221 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
5281 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
5341 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
5401 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
5461 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
5521 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
5581 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagcagatca
5641 attcgcgcgc gaaggcgaag cggcatgcat aatgtgcctg tcaaatggac gaagcaggga
5701 ttctgcaaac cctatgctac tccgtcaagc cgtcaattgt ctgattcgtt accaattatg
5761 acaacttgac ggctacatca ttcacttttt cttcacaacc ggcacggaac tcgctcgggc
5821 tggccccggt gcatttttta aatacccgcg agaaatagag ttgatcgtca aaaccaacat
5881 tgcgaccgac ggtggcgata ggcatccggg tggtgctcaa aagcagcttc gcctggctga
5941 tacgttggtc ctcgcgccag cttaagacgc taatccctaa ctgctggcgg aaaagatgtg
6001 acagacgcga cggcgacaag caaacatgct gtgcgacgct ggcgatatca aaattgctgt
6061 ctgccaggtg atcgctgatg tactgacaag cctcgcgtac ccgattatcc atcggtggat
6121 ggagcgactc gttaatcgct tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg
6181 ccagcagctc cgaatagcgc ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt
6241 cgctgaaatg cggctggtgc gcttcatccg ggcgaaagaa ccccgtattg gcaaatattg
6301 acggccagtt aagccattca tgccagtagg cgcgcggacg aaagtaaacc cactggtgat
6361 accattcgcg agcctccgga tgacgaccgt agtgatgaat ctctcctggc gggaacagca
6421 aaatatcacc cggtcggcaa acaaattctc gtccctgatt tttcaccacc ccctgaccgc
6481 gaatggtgag attgagaata taacctttca ttcccagcgg tcggtcgata aaaaaatcga
6541 gataaccgtt ggcctcaatc ggcgttaaac ccgccaccag atgggcatta aacgagtatc
6601 ccggcagcag gggatcattt tgcgcttcag ccatactttt catactcccg ccattcagag
//
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