| 质粒名称: | pH2B-tdTomato |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0003000 |
| 产品用途: | Expresses H2B-tdTomato in mammalian cells culture |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Top10 |
|
插入基因信息 |
|
| 插入基因名称: | pH2B-tdTomato |
| 物种来源: | synthetic, synthetic |
| 密码子优化: | 未优化 |
pH2B-tdTomato
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pH2B-tdTomato载体质粒基本信息
pH2B-tdTomato质粒图谱载体图谱和pH2B-tdTomato载体序列质粒序列多克隆位点信息
pH2B-tdTomato质粒载体简介
pH2B-tdTomato质粒序列载体序列
LOCUS pH2B-tdTomato 5759 bp DNA circular SYN 19-AUG-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5759)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 10, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5759
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 591..993
/label=MCS
/note="multiple cloning site"
CDS 601..978
/codon_start=1
/label=H2B
/translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
GELAKHAVSEGTKAVTKYTSSK"
primer_bind 983..1015
/label=H2B-tdTomato-F
misc_feature 994..2421
/label=tdTomato
regulatory 994..997
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
primer_bind complement(1402..1431)
/label=mS-S147X RE
primer_bind complement(2128..2157)
/label=mS-S147X RE
primer_bind complement(2401..2439)
/label=H2B-tdTomato-R
polyA_signal 2547..2668
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2549..2576)
/label=Primer 1
rep_origin complement(2675..3130)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3157..3261
/gene="bla"
/label=AmpR promoter
promoter 3263..3620
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3471..3606
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3655..4449
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4681..4728
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 5057..5645
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc
601 atgcctgaac cggcaaaatc cgctccggcc cctaaaaagg gctccaagaa agccgtcacc
661 aaagcccaga agaaagacgg caagaagcgc aagcgcagcc gcaaagagag ctactccatc
721 tacgtgtaca aggtgctgaa gcaggtccac cccgacaccg gcatctcgtc caaggccatg
781 ggcatcatga actccttcgt caacgacatc ttcgagcgca tcgcgggaga ggcttcccgc
841 ctggcgcact acaacaagcg ctccaccatc acatcccgcg agatccagac ggccgtgcgc
901 ctgctgctgc ccggcgagct ggccaagcac gccgtgtccg agggcaccaa ggcggtcacc
961 aagtacacca gctccaagct cgagctcaag cttatggtga gtaagggcga ggaagtgatc
1021 aaagagttca tgcggtttaa ggtgagaatg gaaggaagca tgaacggcca cgagttcgaa
1081 attgagggag aaggagaggg acggccctac gagggcaccc agacagccaa gctgaaagtg
1141 acaaagggcg ggcctctgcc attcgcttgg gacatcctga gcccacagtt tatgtacggc
1201 tccaaggcct atgtgaaaca tccagctgac attcccgatt ataagaaact gagcttcccc
1261 gaggggttta agtgggaaag agtgatgaac ttcgaggacg gaggcctggt gactgtgacc
1321 caggacagct ccctgcagga tgggaccctg atctacaagg tgaaaatgag agggacaaat
1381 tttccccctg atggacctgt gatgcagaag aaaactatgg gatgggaggc ctccaccgaa
1441 aggctgtatc cacgcgacgg ggtgctgaaa ggagaaatcc accaggctct gaagctgaaa
1501 gatgggggac attacctggt ggagttcaag acaatctaca tggccaagaa acctgtgcag
1561 ctgccaggct actattacgt ggacacaaaa ctggatatca cttcacacaa cgaggactac
1621 actattgtgg agcagtatga acggagcgag gggagacacc atctgttcct gggccatggg
1681 actggaagta ccggctcagg gtctagtgga accgcctcaa gcgaggataa caatatggct
1741 gtgatcaaag agttcatgag gtttaaggtg cgcatggagg gcagcatgaa tgggcacgaa
1801 tttgagattg aaggagaggg cgaagggagg ccttacgagg gcacacagac tgccaagctg
1861 aaagtgacca agggaggacc actgcctttc gcttgggata tcctgtctcc tcagtttatg
1921 tacggaagta aggcctatgt caagcatccc gctgacattc ctgattacaa gaaactgtct
1981 ttcccagagg gctttaagtg ggagagagtg atgaattttg aagatggagg cctggtgacc
2041 gtgacacagg actcctctct gcaggatggc actctgatct acaaagtcaa aatgcgcggc
2101 accaattttc cacccgatgg gcccgtgatg cagaagaaaa caatggggtg ggaggccagc
2161 actgaacggc tgtatcctag agacggagtg ctgaagggcg aaatccacca ggccctgaag
2221 ctgaaagacg gcggccacta cctggtggag ttcaaaacca tctacatggc caagaaacca
2281 gtgcagctgc ccggctatta ctatgtggac accaagctgg atatcacatc ccacaatgaa
2341 gactacacca ttgtggaaca gtatgagagg tctgaaggac gccaccatct gtttctgtac
2401 ggcatggatg agctgtataa gtaaagcggc cgcgactcta gatcataatc agccatacca
2461 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
2521 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
2581 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
2641 gtttgtccaa actcatcaat gtatcttaag gcgtaaattg taagcgttaa tattttgtta
2701 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
2761 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
2821 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
2881 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
2941 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
3001 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
3061 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
3121 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
3181 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
3241 taatattgaa aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt
3301 tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
3361 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
3421 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
3481 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
3541 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
3601 gaggcctagg cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt
3661 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat
3721 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
3781 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac
3841 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
3901 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
3961 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
4021 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
4081 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
4141 caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag
4201 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
4261 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
4321 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
4381 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
4441 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
4501 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
4561 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
4621 ctagggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
4681 cggcaataaa aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg
4741 ttcggtccca gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg
4801 cccgcgtttc ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg
4861 cagccaacgt cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga
4921 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
4981 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
5041 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
5101 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
5161 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
5221 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
5281 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
5341 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
5401 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
5461 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
5521 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
5581 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
5641 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
5701 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccatgcat
//
pH2B-tdTomato载体图谱质粒图谱pdf版和pH2B-tdTomato载体序列质粒序列等相关资料下载
pH2B-tdTomato质粒载体应用举例
- 上一篇：pH2B-mRuby3
- 下一篇：pH2B-mEGFP
