pCAMBIA1301-proMpEF1a-MpCDF-5xMyc

LOCUS       pCAMBIA1301-proMpEF1a-MpCDF-5xMyc          13508 bp DNA     circular SYN 03-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..13508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1070..1699
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2128..3201
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3267..3461
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     misc_feature    3805..3945
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4131..4719)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4806..5600)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    6025..6049
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    6127..6301
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(6341..7366)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(7434..8111)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    8302..8323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8338..8368
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8376..8392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8400..8416
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8432..10160
                     /label=proMpEF1alpha
     gene            10176..12698
                     /label=Mp1g18540
     CDS             12714..12743
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             12753..12782
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             12792..12821
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             12831..12860
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             12894..12923
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      12979..13231
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    13253..13277
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ctgccctttt cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc
       61 gactagaacc ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat
      121 gcccgcgtca gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc
      181 ggctgcacca agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg
      241 gccaggatgc ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc
      301 ctggcccgca gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg
      361 ggcctgcgta gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg
      421 ttgaccgtgt tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg
      481 agcgggcgcg aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc
      541 ccggcacaga tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag
      601 gcggctgcac tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag
      661 gaagtgacgc ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag
      721 gccgacgccc tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc
      781 aggacggcca ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg
      841 ccgggtacgt gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg
      901 ccggtttgtc tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg
      961 agcgccgccg tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg
     1021 ctgcgtatat gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat
     1081 cgctgtactt aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg
     1141 cgccctgcaa ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc
     1201 ccgcgattgg gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc
     1261 gacgattgac cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc
     1321 gccccaggcg gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc
     1381 ggtgcagcca agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca
     1441 gcgcattgag gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa
     1501 aggcacgcgc atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct
     1561 tgagtcccgt atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt
     1621 tcttgaatca gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat
     1681 taaatcaaaa ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg
     1741 ctaagtgccg gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca
     1801 gacacgccag ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg
     1861 aagatgtacg cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg
     1921 cagctaccag agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg
     1981 aggcggcatg gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg
     2041 aggaacgggc ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac
     2101 tggaaccccc aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa
     2161 atcggcgcgg cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag
     2221 cggcaacgca tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga
     2281 atccgcaaag aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc
     2341 aagggcgacg agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat
     2401 agtcgcagca tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc
     2461 gaggtgatcc gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc
     2521 atggccagtg tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc
     2581 atgaaccgat accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt
     2641 gcggacgtac tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta
     2701 gaaacctgca ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag
     2761 aacggccgcc tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta
     2821 aagagcgaaa ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc
     2881 gagatcacag aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc
     2941 gatcccggca tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa
     3001 gccagatggt tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag
     3061 ttctgtttca ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag
     3121 gaggaggcgg ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc
     3181 gaagcatccg ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg
     3241 gaaaaaggtc gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg
     3301 tacattggga accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca
     3361 cacatgtaag tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta
     3421 aaacttatta aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca
     3481 gccgaagagc tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct
     3541 tcgcgtcggc ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat
     3601 ctaccagggc gcggacaagc cgcgccgtcg ccactcgacc gccggcgccc acatcaaggc
     3661 accctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     3721 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     3781 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     3841 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg
     3901 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     3961 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     4021 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     4081 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     4141 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     4201 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     4261 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     4321 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     4381 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     4441 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     4501 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     4561 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     4621 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     4681 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     4741 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcattct
     4801 aggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     4861 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     4921 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     4981 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     5041 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     5101 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     5161 agtaagtaat ccaattcggc taagcggctg tctaagctat tcgtataggg acaatccgat
     5221 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgataat cttttcaggg
     5281 ctttgttcat cttcatactc ttccgagcaa aggacgccat cggcctcact catgagcaga
     5341 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     5401 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     5461 tccgtcattt ttaaatatag gttttcattt tctcccacca gcttatatac cttagcagga
     5521 gacattcctt ccgtatcttt tacgcagcgg tatttttcga tcagtttttt caattccggt
     5581 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     5641 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     5701 ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa gttggcgtat aacatagtat
     5761 cgacggagcc gattttgaaa ccgcggtgat cacaggcagc aacgctctgt catcgttaca
     5821 atcaacatgc taccctccgc gagatcatcc gtgtttcaaa cccggcagct tagttgccgt
     5881 tcttccgaat agcatcggta acatgagcaa agtctgccgc cttacaacgg ctctcccgct
     5941 gacgccgtcc cggactgatg ggctgcctgt atcgagtggt gattttgtgc cgagctgccg
     6001 gtcggggagc tgttggctgg ctggtggcag gatatattgt ggtgtaaaca aattgacgct
     6061 tagacaactt aataacacat tgcggacgtt tttaatgtac tgaattaacg ccgaattaat
     6121 tcgggggatc tggattttag tactggattt tggttttagg aattagaaat tttattgata
     6181 gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa cacatgagcg
     6241 aaaccctata ggaaccctaa ttcccttatc tgggaactac tcacacatta ttatggagaa
     6301 actcgagctt gtcgatcgac agatccggtc ggcatctact ctatttcttt gccctcggac
     6361 gagtgctggg gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga
     6421 cggccgcgct tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga
     6481 ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct
     6541 gatagagttg gtcaagacca atgcggagca tatacgcccg gagtcgtggc gatcctgcaa
     6601 gctccggatg cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc
     6661 tccagaagaa gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt
     6721 caatgaccgc tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt
     6781 gcacgaggtg ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct
     6841 gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat
     6901 cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga
     6961 atgggccgaa cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atagcctccg
     7021 cgaccggttg tagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct
     7081 gtgcacggcg ggagatgcaa taggtcaggc tctcgctaaa ctccccaatg tcaagcactt
     7141 ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac
     7201 catcggcgca gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag
     7261 cacgagattc ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga
     7321 tcagaaactt ctcgacagac gtcgcggtga gttcaggctt tttcatatct cattgccccc
     7381 cgggatctgc gaaagctcga gagagataga tttgtagaga gagactggtg atttcagcgt
     7441 gtcctctcca aatgaaatga acttccttat atagaggaag gtcttgcgaa ggatagtggg
     7501 attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc tttgaagacg
     7561 tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca tctttgggac
     7621 cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga tggcatttgt
     7681 aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct gggcaatgga
     7741 atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc ctttggtctt
     7801 ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc accatgttat
     7861 cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc
     7921 tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga acgatagcct
     7981 ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact gtccttttga
     8041 tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt accctttgtt
     8101 gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc ttggagtaga
     8161 cgagagtgtc gtgctccacc atgttggcaa gctgctctag ccaatacgca aaccgcctct
     8221 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     8281 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     8341 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     8401 aggaaacagc tatgaccatg attacgaatt ccaaatgagt cacacacatt gttgagagac
     8461 atatcaaaat aactcaattg taaggtaagt tgatagattt cccagttaaa tgcattattt
     8521 taacaatatt tgtatctcaa attttcaaaa tcatatcaaa tttgcagatc ttgatgatga
     8581 ttcacaactc tgtcttcgat ttttgattgt taacgggtat cgatatttgg gtaacttaaa
     8641 aagaatttgc taaagatctt aaaaaacaga ttaaaaaaac ctttcaaaat agctaatcct
     8701 ttaaatccgt ttcacaatat aatgctcatt gatgataatc gaaaagattc cattttaaag
     8761 tgcatataaa ttgcttaatt atctctttct gtctcgtaat attttgttct ctcctattgg
     8821 atcggacccg catcttctcg atcagttcac tatactaata tttggtcaac ttcaagagag
     8881 attagaactt cgttctccaa agtgtaaagg ccgacaagca gaaaaggaaa cttcaatcag
     8941 aagccttcta ggttttcccg cgtcccaaaa agcgaaggga ggacggggag gacggtgagg
     9001 ccagaagaag aaagcaacag taaaatctca tgtgattgcg tgcatgtcat ggatctatgg
     9061 gatgctagtc caccgtttgg aagtatgtgc agaaggagaa aggcgcggga ggccaaaagc
     9121 gacttcggcc acctctctac tttctagaaa gtttgctcgc agcttgtaat aagcgcggaa
     9181 aaggtactcg aaaaaccgga gcaatcggtg tggtatcagc agtcgggttc gctaaaccta
     9241 cgtcttctta ttttggaatg ttaaattgaa agggcgtgcc ggccctaagc acggacgatg
     9301 tgacagacgg aggagtgtga gcaattcgga tgacgatgct ggccggcagt cacgacaaag
     9361 agtccgaagg ggcatggaca gggcaatata taaatggaaa agaggctttg aatattccta
     9421 acccttcgtc gtcggcctcc caacttgccc tcgcatgctg aattggccgc ctatctgcga
     9481 atggccggcc gccgacccta agtcgcaata acttgaacgg agccgatgca aagtccttat
     9541 cgaacgctcg ttattataaa cggggcagac ctaggtattt aattatatca aaaccctccg
     9601 caatttcgct aatcattccc taatttcttc tactttggcg tctagtgctg tggtcgcgct
     9661 tgctctcaaa gctcgctggg aggcagtgga gcgtctggct taaaatcgat cgccgcaacc
     9721 ctagagttcc tttcacttct ttctctcttg cgtggagagc atcgacatcg aagagctgca
     9781 tcttgtcttc tggtatgcgc cttgtgccct cgtctctgta cgtcgttacg attgctcgat
     9841 attgtgattg tctagacctg aaacccgact ggagctttta cgtggtgtcg attggggcct
     9901 cgagcgagtg attttttagg ttcgttttag ccaagtacat cccactttgg tcagtcctgt
     9961 tatctcggta aggtgttcag ttcggtctct ggtgcggagg tgcctcgtaa gtttcctgca
    10021 tgatagattt gtctatcctt gacttgaaga ggagtcgaag gtcagcgagg ttggctgtgt
    10081 gctatggacg tcggtgctcc ttggttccgt gcttctatgg gtgttgcctc aacagtattg
    10141 atgtgcctgc agaaaggttg ggtaccgaaa agcttatgat ggattcaccg atcaagttgt
    10201 tcgggaaaac aattgctaaa gctgccgctt ccgcattcac aacatcatca ggtgaagcag
    10261 acgacgctga agcagtcccg actacgagca gtgggggctg ggttgatttg ccatcgtcgt
    10321 cgcaggtgtg tacttccctt ccagctcttt gaatgtatgc aagctgatct tgcctacctg
    10381 acgagcattc gagcagccct tcgtcgctcg tcacagatgc gtttccgtgt cttttgcata
    10441 gccactcctc aggagggctc aggctccgcg cctgcgtgtt tcaatcgatg cctgcgtgag
    10501 tgttttgtgc gctttatgaa ttggttcaat cgccgtatag gataggatag tataggatgc
    10561 gatatgcttt ctttccatgg aggggtggct cgccgagcac gtcggcagcc ggtggcgaag
    10621 tcgctagtcc gtctcaatgt cgcctgcgcc agagcagagc atcgcgggcc gtcgggactg
    10681 acgtgggcgg gcgtgcaatt tctgtctggg atgtgcagga tgtgaaggaa aatggagcgg
    10741 aaagcagcag actgggggag atcaatggag gcggggtgac gttcacgagg caggaggtga
    10801 aacgaggatc gtcgcccgac cggacgtcgt cggcgtgcaa cagcgcggag gaggagaagc
    10861 tgtgcgcgtc gccgcgaggc ggaggcgggg ccacgggggg agcaggagca gagctgacgc
    10921 cctcggcctc ggggggcgag gcgggcgcgg gcgacgacga gggcagcgag gaggaggatg
    10981 gatcgggcat gaatgggcgc agtaaaaagg atggcggcga cgatggcggc aagggagggg
    11041 agaagatgcc ccgacggccc gagaatgctg ttgcgtgtcc tcgatgccat agttttgaga
    11101 cgaagttttg ttactacaac aattacaacg tcaatcagcc ccggcacttc tgcaagcact
    11161 gccagcgcta ctggaccgcg ggcggcacgc tgcgcaacgt gccggtgggc gccgggcggc
    11221 gcaagaacaa gcacggcagc accatcgcgc cgcgcggcca gacgcccgag gtcggcaccg
    11281 tgcgcagcga cgcgcccgac atcgcctcgc cgctcatcgc gtccaagggc ggcgtcgggc
    11341 ccgtgcccgt gcaggtcgcg gtcgtgcagg ccgcgggcag caagggcggg attgtgcgca
    11401 tggccgtgcc tgcggccagc ggcgacccct cggttctgcc gtttctgcga atgcagccct
    11461 cgccgatgga caccgcctcc atcttcatgg cctcgtccga tgccggcacc tcgagcgaca
    11521 cggtcaccgt ggcctgcaag cgcggccggc tggccactcc cgggttcagc gacgcatcca
    11581 caggtgccaa cgacatggag cagcagcagc agcagcagca gcagccgtct tgggggcagc
    11641 agtcgtcgac gaatgatgaa tccgcctgct cgcccctcac gtcggcgaat ggcaatgttg
    11701 tggtgcatga gattaagggc gaacccgggt cagcgtgtca gatgatgggt gtggcagcgg
    11761 tatcaggagt gggggggccc gagcagcagc agcagcagca gcaacaacag caacagcagc
    11821 agcagcagca gcagcatcct gcgacgaaca acggcatggc cggtccaggg ggttgttggt
    11881 ctgctgccgg tccgcaatcg agcgtacctc cgcccggggc cgcgggagcc ccttacggat
    11941 tctataacgg aggatggccg tatgggtaca acgtgggctg gagcgggcct cctgcattct
    12001 gcgccggggg agttgccact gcgggcacgg gcatgccggg ccctcccccg ccgggcaaca
    12061 tcgctgcgtg gaatggagcc gggggcagcg tctggaccgg ggttccttgg cccatgccgg
    12121 gcctggcttg gggccccccc tgggctatgg cgccgtgggc catgagtgcg ggtcacggtg
    12181 gggctagcat ggggagcatt acgcagacga tttcgatgag ctccggtgct gctgctggca
    12241 tggtcactgt gccggtgccc tgcagtacga ctgccagtgg tggtggtcct gcgtccgctg
    12301 cgcccccgtc atcgccctca tcatcactgg gcaagcattc gcgcgggggg ccgccggtgg
    12361 gggcccctgc atcgtctgcc gcagcagctg ctgcttgcaa tggcgcagga ggagcaggtg
    12421 ggggaggagg aggcggcgga ggaggccgca tggagggagg cctgtgggcg cccaaatcgc
    12481 tgcgaatggc tgatcctgag gaggcaatgc gaagttcggt ttggaggata ctgggagtcg
    12541 gttcccgagc cgacatgacg acggccagca catttaaagc gttccagcct agaaacgaga
    12601 gtaagaagat ttccgatgat gatcaacaag cagtgcaagc gtcgttgcat gcgaaccctg
    12661 cagcgatgtc tcgttccatg aatttccacg agagtaacgg atccggtacc atggagcaaa
    12721 agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact
    12781 tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagcaaaagc
    12841 tcatttctga agaggacttg aatgaaatgg agagcttggg cgacctcacc atggagcaaa
    12901 agctcatttc tgaagaggac ttgaactcgg tataagagct cgaatttccc cgatcgggtg
    12961 accagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat
    13021 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta
    13081 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg
    13141 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta
    13201 tcgcgcgcgg tgtcatctat gttactagat cgggaattaa actatcagtg tttgacagga
    13261 tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg atatttaaaa
    13321 gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca cagggttccc
    13381 ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc ggcttctgac
    13441 gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt acgcgacagg
    13501 ctgccgcc
//