| 质粒名称: | pSL1205 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002426 |
| 产品用途: | Mammalian PhCMV-driven constitutive MCP expression vector (PhCMV-MCP-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | PhCMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
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pSL1205
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1205载体质粒基本信息
pSL1205质粒图谱载体图谱和pSL1205载体序列质粒序列多克隆位点信息
pSL1205质粒载体简介
pSL1205质粒序列载体序列
LOCUS pSL1205 5799 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5799)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5799
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 880..923
/label=oSCL2210
primer_bind 886..918
/label=Oscl32-1
primer_bind 886..918
/label=oSCL1118
primer_bind 901..918
/label=Oscl42-1
regulatory 901..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 902..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 910..1299
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
CDS 910..1296
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
VKAMQGLLKDGNPIPSAIAANSGIY"
misc_feature 1023^1024
/label=Feature 16
misc_feature 1068^1069
/label=Feature 17
misc_feature 1191^1192
/label=Feature 18
primer_bind complement(1276..1319)
/label=oSCL2211
primer_bind complement(1276..1305)
/label=Oscl32-2
primer_bind complement(1276..1296)
/label=oSCL093
terminator 1396..1623
/label=bGH PA
polyA_signal 1399..1623
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1669..2097
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2111..2440
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2291..2426
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 2291..2368
/label=SV40 ORI
CDS 2507..3301
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2510..3301
/label=Kan/neoR
polyA_signal 3475..3596
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3641..3661)
/label=M13-rev
primer_bind complement(3645..3661)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(3667..3689)
/label=LacO
protein_bind 3669..3685
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3693..3723)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(3694..3723)
/label=lac
protein_bind 3738..3759
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4047..4632)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4803..5663)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(4806..5465)
/label=AmpR
promoter complement(5664..5768)
/gene="bla"
/label=AmpR promoter
promoter complement(5705..5733)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg gacaggggat
961 gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag ctccaactca
1021 cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctagtgccca gaagagaaag
1081 tataccatca aggtggaggt ccccaaagtg gctacccaga cagtgggcgg agtcgaactg
1141 cctgtcgccg cttggaggtc ctacctgaac atggagctca ctatcccaat tttcgctacc
1201 aattctgact gtgaactcat cgtgaaggca atgcaggggc tcctcaaaga cggtaatcct
1261 atcccttccg ccatcgccgc taactcaggt atctactaag gatccactag tccagtgtgg
1321 tggaattctg cagatatcca gcacagtggc ggccgctcga gtctagaggg cccgtttaaa
1381 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
1441 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
1501 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
1561 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
1621 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
1681 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
1741 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
1801 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
1861 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
1921 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
1981 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
2041 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
2101 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
2161 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
2221 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
2281 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
2341 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
2401 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
2461 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
2521 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
2581 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
2641 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
2701 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
2761 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
2821 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
2881 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
2941 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
3001 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
3061 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
3121 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
3181 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
3241 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
3301 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
3361 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
3421 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
3481 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
3541 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
3601 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
3661 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
3721 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
3781 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
3841 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
3901 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
3961 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
4021 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
4081 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
4141 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
4201 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
4261 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
4321 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
4381 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
4441 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
4501 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
4561 aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa
4621 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
4681 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
4741 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
4801 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
4861 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
4921 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
4981 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
5041 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
5101 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
5161 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
5221 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
5281 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
5341 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
5401 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
5461 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
5521 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
5581 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
5641 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
5701 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
5761 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
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