| 质粒名称: | pDG196 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002902 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for hPEST-tagged NanoLuc (PhCMV-NanoLuc-hPEST-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NanoLuc, hPEST |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pDG196
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pDG196载体质粒基本信息
pDG196质粒图谱载体图谱和pDG196载体序列质粒序列多克隆位点信息
pDG196质粒载体简介
pDG196质粒序列载体序列
LOCUS pDG196 6125 bp DNA circular SYN 21-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6125)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6125
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 826..845
/label=Primer 1
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 887..944
/label=oSCL1108
misc_feature 895..911
/label=MCS
/note="multiple cloning site"
primer_bind 897..944
/label=oSCL1314
primer_bind 898..936
/label=Primer 14
primer_bind 899..936
/label=Primer 7
primer_bind 899..936
/label=Primer 16
primer_bind 899..936
/label=Primer 19
primer_bind 899..936
/label=Primer 26
primer_bind 911..936
/label=Primer 1a
primer_bind 911..936
/label=Primer 9
promoter 911
/label=P_SV40
primer_bind 917..944
/label=oSCL1846
primer_bind 917..940
/label=oSCL1848
primer_bind 917..936
/label=Primer 3
primer_bind 921..944
/label=oSCL084
misc_feature 923..991
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind complement(990..1009)
/label=Primer 8
primer_bind 1008..1029
/label=Primer 17
misc_feature 1013..1522
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
primer_bind 1013..1032
/label=Primer 5
misc_feature 1043..1045
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1502..1522)
/label=oSCL1849
primer_bind complement(1503..1542)
/label=Primer 27
primer_bind complement(1503..1523)
/label=Primer 15
primer_bind complement(1503..1522)
/label=Primer 4
primer_bind complement(1503..1522)
/label=Primer 6
primer_bind complement(1503..1522)
/label=Primer 10
primer_bind complement(1503..1522)
/label=Primer 18
primer_bind complement(1503..1522)
/label=Primer 20
primer_bind complement(1512..1522)
/label=oSCL085
primer_bind complement(1512..1522)
/label=oSCL1109
primer_bind 1523..1542
/label=Primer 1`b
CDS 1526..1645
/codon_start=1
/product="PEST degradation sequence from mouse ornithine
decarboxylase"
/label=hPEST
/note="human codon-optimized"
/translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
primer_bind complement(1629..1694)
/label=oIU57-2
primer_bind complement(1629..1668)
/label=Primer 2a
primer_bind complement(1648..1666)
/label=Primer 18
primer_bind complement(1648..1665)
/label=Primer 6
misc_feature 1654..1699
/label=MCS
/note="multiple cloning site"
primer_bind complement(1699..1718)
/label=Primer 2
polyA_signal 1725..1949
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 1995..2423
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2437..2766
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2617..2752
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2752..2771
/label=Primer 1a
CDS 2833..3627
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3801..3922
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(4373..4958)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5129..5989)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5990..6094)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgactag tgagacagac acactcctgc tatgggtact
961 gctgctctgg gttccaggtt ccactggtga cgctagtggt ggttctggta tggtcttcac
1021 actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct
1081 tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca
1141 aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta
1201 tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc
1261 tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt
1321 tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg
1381 caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct
1441 gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg
1501 gctgtgcgaa cgcattctgg cgaattctca cggcttccct cccgaggtgg aggagcaggc
1561 cgccggcacc ctgcccatga gctgcgccca ggagagcggc atggatagac accctgctgc
1621 ttgcgccagc gccaggatca acgtctaaga attctgcaga tatccagcac agtggcggcc
1681 gctcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
1741 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
1801 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
1861 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
1921 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
1981 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
2041 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
2101 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
2161 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
2221 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
2281 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
2341 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
2401 ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg gtgtggaaag
2461 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
2521 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2581 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
2641 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
2701 gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
2761 tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga gacaggatga
2821 ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg
2881 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg
2941 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc
3001 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct
3061 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa
3121 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg
3181 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa
3241 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat
3301 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg
3361 cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc
3421 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac
3481 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg
3541 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc
3601 tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgaaatg accgaccaag
3661 cgacgcccaa cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg
3721 gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc
3781 tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca
3841 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
3901 ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg
3961 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
4021 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
4081 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
4141 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
4201 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
4261 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
4321 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
4381 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
4441 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
4501 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
4561 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
4621 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
4681 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
4741 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
4801 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
4861 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ttttttgttt
4921 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
4981 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
5041 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
5101 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
5161 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
5221 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
5281 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
5341 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
5401 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
5461 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
5521 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
5581 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
5641 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
5701 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
5761 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
5821 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
5881 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
5941 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
6001 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
6061 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
6121 acgtc
//
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