pAAVLINK-EF1alpha-HA-5'-ATP7A

LOCUS       pAAVLINK-EF1alpha-HA-5'-ATP7A           6234 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6234)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6234
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..3374
                     /codon_start=1
                     /gene="ATP7A"
                     /gene_synonym="DSMAX; HMNX; MK; MNK; SMAX3"
                     /product="copper-transporting ATPase 1 isoform 1"
                     /EC_number="7.2.2.8"
                     /label=ATP7A
                     /note="isoform 1 is encoded by transcript variant 1;
                     Menkes disease-associated protein; copper pump 1; 
                     copper-transporting ATPase 1; Cu++-transporting P-type 
                     ATPase; ATPase, Cu++ transporting, alpha polypeptide"
                     /db_xref="CCDS:CCDS35339.1"
                     /db_xref="GeneID:538"
                     /db_xref="HGNC:HGNC:869"
                     /db_xref="MIM:300011"
                     /protein_id="NP_000043.4"
                     /translation="MDPSMGVNSVTISVEGMTCNSCVWTIEQQIGKVNGVHHIKVSLEE
                     KNATIIYDPKLQTPKTLQEAIDDMGFDAVIHNPDPLPVLTDTLFLTVTASLTLPWDHIQ
                     STLLKTKGVTDIKIYPQKRTVAVTIIPSIVNANQIKELVPELSLDTGTLEKKSGACEDH
                     SMAQAGEVVLKMKVEGMTCHSCTSTIEGKIGKLQGVQRIKVSLDNQEATIVYQPHLISV
                     EEMKKQIEAMGFPAFVKKQPKYLKLGAIDVERLKNTPVKSSEGSQQRSPSYTNDSTATF
                     IIDGMHCKSCVSNIESTLSALQYVSSIVVSLENRSAIVKYNASSVTPESLRKAIEAVSP
                     GLYRVSITSEVESTSNSPSSSSLQKIPLNVVSQPLTQETVINIDGMTCNSCVQSIEGVI
                     SKKPGVKSIRVSLANSNGTVEYDPLLTSPETLRGAIEDMGFDATLSDTNEPLVVIAQPS
                     SEMPLLTSTNEFYTKGMTPVQDKEEGKNSSKCYIQVTGMTCASCVANIERNLRREEGIY
                     SILVALMAGKAEVRYNPAVIQPPMIAEFIRELGFGATVIENADEGDGVLELVVRGMTCA
                     SCVHKIESSLTKHRGILYCSVALATNKAHIKYDPEIIGPRDIIHTIESLGFEASLVKKD
                     RSASHLDHKREIRQWRRSFLVSLFFCIPVMGLMIYMMVMDHHFATLHHNQNMSKEEMIN
                     LHSSMFLERQILPGLSVMNLLSFLLCVPVQFFGGWYFYIQAYKALKHKTANMDVLIVLA
                     TTIAFAYSLIILLLAMYERAKVNPITFFDTPPMLFVFIALGRWLEHIAKGKTSEALAKL
                     ISLQATEATIVTLDSDNILLSEEQVDVELVQRGDIIKVVPGGKFPVDGRVIEGHSMVDE
                     SLIT"
     misc_feature    2190..2195
                     /label=SDS
     misc_feature    2475..2480
                     /label=SDS
     misc_feature    3238..3243
                     /label=SDS
     misc_feature    3304..3309
                     /label=SDS
     intron          3375..3415
                     /label=Splicing Donor
     misc_feature    3419..3452
                     /label=lox2272-JT1571
     repeat_region   3497..3637
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3497..3626
                     /label=AAV2 ITR
     repeat_region   3498..3638
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3712..4167
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4449..4553
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4554..5414
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5585..6173
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggatccaagt atgggtgtga attctgttac
      781 catttctgtt gagggtatga cttgcaattc ctgtgtttgg accattgagc agcagattgg
      841 aaaagtgaat ggtgtgcatc acattaaggt atcactggaa gaaaaaaatg caactattat
      901 ttatgaccct aaactacaga ctccaaagac cctacaggaa gctattgatg acatgggctt
      961 tgatgctgtt atccataatc ctgaccctct ccctgtttta actgacacct tgtttctgac
     1021 tgttacggcg tcactgactt tgccatggga ccatatccaa agcacattgc tgaagaccaa
     1081 gggtgtgaca gacattaaaa tttaccctca gaaaagaact gtagcagtga caataatccc
     1141 ttctatagtg aatgccaatc agataaaaga gctggttcca gaactcagtt tagatactgg
     1201 gacactggag aaaaagtcag gagcttgtga agatcatagt atggctcaag ctggtgaagt
     1261 cgtgctgaag atgaaagtgg aagggatgac ctgccattca tgtactagca ctattgaagg
     1321 aaaaattggg aaactgcaag gtgttcagcg aattaaagtc tccctggaca atcaagaagc
     1381 tactattgtt tatcaacctc atcttatctc agtagaggaa atgaaaaagc agattgaagc
     1441 tatgggcttt ccagcatttg tcaaaaagca gcccaagtac ctcaaattgg gagctattga
     1501 tgtagaacgt ctaaagaaca caccagttaa atcctcagaa gggtcacagc aaaggagtcc
     1561 atcatatacc aatgattcaa cagccacttt catcattgat ggcatgcatt gtaaatcatg
     1621 tgtgtcaaat attgaaagta ctttatctgc actccaatat gtaagcagca tagtagtttc
     1681 tttagagaat aggtctgcca ttgtgaagta taatgcaagc tcagtcactc cagaatccct
     1741 gagaaaagca atagaggctg tatcaccggg gctatataga gttagtatca caagtgaagt
     1801 tgagagtacc tcaaactctc cctccagctc atctcttcag aagattcctt tgaatgtagt
     1861 tagccagcct ctgacacaag aaactgtgat aaacattgat ggcatgactt gtaattcctg
     1921 tgtgcagtct attgagggtg tcatatcaaa aaagccaggt gtaaaatcca tacgagtctc
     1981 ccttgcaaat agcaatggga ctgttgagta tgatcctcta ctaacctctc cagaaacgtt
     2041 gagaggagca atagaagaca tgggatttga tgctaccttg tcagacacga atgagccgtt
     2101 ggtagtaata gctcagcctt catcggaaat gccgcttttg acttcaacta atgaatttta
     2161 tactaaaggg atgacaccag ttcaagacaa ggaggaagga aagaattcat ctaagtgtta
     2221 catacaggtc actggcatga cttgcgcttc ctgtgtagca aacattgaac ggaatttaag
     2281 gcgggaagaa ggaatatatt ctatacttgt ggccctgatg gctggcaagg cagaagtaag
     2341 gtataatcct gctgttatac aacccccaat gatagcagag ttcatccgag aacttggatt
     2401 tggagccact gtgatagaaa atgctgatga aggagatggt gttttggaac ttgttgtgag
     2461 gggaatgacg tgtgcctcct gcgtacataa aatagagtct agtctcacaa aacacagagg
     2521 gatcctatac tgctccgtgg ccctggcaac caacaaagca catattaaat atgacccaga
     2581 aattattggt cctagagata ttatccatac aattgaaagc ttaggttttg aagcttcttt
     2641 ggtcaagaag gatcggtcag caagtcactt agatcataaa cgagaaataa gacaatggag
     2701 acggtctttt cttgtgagtc tgtttttctg tattcctgta atggggctga tgatatatat
     2761 gatggttatg gaccaccact ttgcaactct tcaccataat caaaacatga gtaaagaaga
     2821 aatgatcaac cttcattctt ctatgttcct ggagcgccag attcttccag gattgtctgt
     2881 tatgaatttg ctgtcctttt tattgtgtgt acctgtacag tttttcggag gctggtactt
     2941 ctacattcag gcttataaag cactgaagca taagacagca aatatggacg tactgattgt
     3001 gctggcaacc accattgcat ttgcctactc tttgattatt cttctacttg caatgtatga
     3061 gagagccaaa gtgaacccta ttactttctt tgacacaccc cctatgctgt ttgtgtttat
     3121 tgcactaggc cgatggctgg aacatatagc aaagggcaaa acatcagagg ctcttgcaaa
     3181 gttaatttca ctacaagcta cagaagcaac tattgtaact cttgattctg ataatatcct
     3241 cctcagtgaa gaacaagtgg atgtggaact tgtacaacgt ggagatatca ttaaagtagt
     3301 tccaggaggc aaatttccag tggatggtcg tgttattgaa ggacattcta tggtagatga
     3361 gtccctcatc acaggtaagt atcaaggtta caagacaggt ttaaggagac caataagcta
     3421 ttattcgtat aggatacttt atacgaagtt attaagttat ggagaccggt gtggcacgtg
     3481 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     3541 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     3601 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
     3661 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
     3721 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     3781 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     3841 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     3901 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
     3961 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4021 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
     4081 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4141 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
     4201 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4261 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4321 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4381 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4441 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4501 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4561 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4621 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4681 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4741 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4801 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4861 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4921 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4981 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5041 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5101 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     5161 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     5221 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     5281 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     5341 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5401 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5461 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5521 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5581 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5641 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5701 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5761 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5821 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5881 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5941 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     6001 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     6061 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     6121 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     6181 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//