| 质粒名称: | pKGW-DR-MGW |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000446 |
| 产品用途: | Destination vector for multiple Gateway cloning by attR4 and attR3 recombination sites. pKGW-DR-MGW can be used for the localization of promoter-reporter or translational fusions together with visualization of cellular auxin response maxima (DR5-mRuby3-H2B). NOTE: you need to use three different entry vectors (one with attL4 and attR1 recombination sites, one with attL1 and attL2, and one with attR2 and attL3). PROPAGATION: use spectinomycin (50 mkg/ml) together with the chloramphenicol (30 mkg/ml) for empty plasmid propagation both in E. coli and in Agrobacterium strains. Chloramphenicol allows saving the pressure against ccdB cassette. |
| 存储实验室: | Demchenko Lab (BIN RAS) |
|
载体骨架基本信息 |
|
| 载体名称: | pKGW-MGW |
| 表达系统: | 植物表达 |
| 启动子: | pNOS, DR5 |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 壮观霉素,氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | CcdB survival |
|
插入基因信息 |
|
| 插入基因名称: | DR5-mRuby3-H2B-AtACT2t cassette |
| 物种来源: | Plants |
| 密码子优化: | 植物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Lateral root initiation in cucumber (Cucumis sativus): what does the expression pattern of Rapid Alkalinization Factor 34 (RALF34) tell us?
Kiryushkin A.S., Ilina E.L., Guseva E.D., Pawlowski K., Demchenko K.N.
Int. J. Mol. Sci. 24, (2023)
|
pKGW-DR-MGW
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pKGW-DR-MGW载体质粒基本信息
pKGW-DR-MGW质粒图谱载体图谱和pKGW-DR-MGW载体序列质粒序列多克隆位点信息
pKGW-DR-MGW质粒载体简介
pKGW-DR-MGW质粒序列载体序列
LOCUS pKGW-DR-MGW 11868 bp DNA circular SYN 27-FEB-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pKGW-DR-MGW
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11868)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Sep 26, 2026 from SnapGene 6.0.2
https://www.snapgene.com
COMMENT This file is created by Vector NTI
http://www.biofeng.com
ORIGDB|GenBank
VNTDATE|-14633325|
VNTDBDATE|-14952459|
LSOWNER|
VNTNAME|pKGW-DR-MGW|
VNTAUTHORNAME|Alexey|
VNTOAUTHORNAME|UNKNOWN|
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FEATURES Location/Qualifiers
source 1..11868
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_signal 27..150
/label=attR3
/note="/vntifkey=87"
promoter 176..206
/label=lacUV5 promoter
/label=lac\UV5\promoter
/note="E. coli lac promoter with an up mutation"
/note="/vntifkey=30"
CDS 260..919
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/note="/vntifkey=4"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
promoter 945..1038
/label=promoter of ccdA/ccdB operon
/label=promoter\of\ccdA/ccdB\operon
/note="/vntifkey=30"
CDS 1039..1259
/label=ccdA (mutated)
/label=ccdA\(mutated)
/note="/vntifkey=4"
variation 1098..1099
/label=ccdA frame-shifting nucleotides
/label=ccdA\frame-shift\nucleotides
/note="/vntifkey=62"
CDS 1261..1566
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/note="/vntifkey=4"
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
misc_signal 1607..1731
/gene="mutant version of attR"
/label=attR4
/note="recombination site for the Gateway(R) LR reaction"
/note="/bound_moiety=LR Clonase(TM)"
/note="/vntifkey=87"
primer_bind complement(1750..1766)
/label=M13 rev
/label=M13\rev
/note="common sequencing primer, one of multiple similar
variants"
/note="/vntifkey=28"
misc_signal 1833..1857
/label=RB T-DNA repeat
/label=RB\T-DNA\repeat
/note="right border repeat from nopaline C58 T-DNA"
/note="/vntifkey=87"
misc_feature 3157..3786
/direction=RIGHT
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/label=pVS1\StaA
/label=nonstandard type: insertion_seq
/note="/vntifkey=14"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 4220..5287
/direction=RIGHT
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/label=pVS1\RepA
/label=nonstandard type: insertion_seq
/note="/vntifkey=14"
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
misc_feature 5891..6031
/label=bom
/label=nonstandard type: insertion_seq
/note="basis of mobility region from pBR322"
/note="/vntifkey=14"
rep_origin complement(6217..6805)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="/vntifkey=33"
CDS complement(7049..7840)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/note="/vntifkey=4"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
misc_signal 8368..8392
/label=LB T-DNA repeat
/label=LB\T-DNA\repeat
/note="left border repeat from nopaline C58 T-DNA"
/note="/vntifkey=87"
terminator 8485..8737
/label=NOS terminator
/label=NOS\terminator
/note="nopaline synthase terminator and poly(A) signal"
/note="/vntifkey=43"
CDS complement(8777..9571)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/note="/vntifkey=4"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter complement(9605..9784)
/label=NOS promoter
/label=NOS\promoter
/note="nopaline synthase promoter"
/note="/vntifkey=30"
promoter 9880..10107
/label=DR5
/note="/vntifkey=29"
CDS 10162..10874
/label=mRuby3
/note="/vntifkey=4"
CDS 10901..11280
/label=H2B
/note="/vntifkey=4"
terminator 11350..11842
/label=AtACTIN2 terminator
/label=AtACTIN2\terminator
/note="/vntifkey=43"
ORIGIN
1 cgacgtcacg acggccagtg aattatcaac tatgtataat aaagttgaac gagaaacgta
61 aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga ctacataata
121 ctgtaaaaca caacatatcc agtcactatg gcggccgcat taggcacccc caggctttac
181 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
241 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
301 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
361 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
421 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
481 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
541 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
601 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
661 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
721 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
781 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
841 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
901 gtggcagggc ggggcgtaat ctagaggatc cggcttacta aaagccagat aacagtatgc
961 gtatttgcgc gctgattttt gcggtataag aatatatact gatatgtata cccgaagtat
1021 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
1081 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
1141 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
1201 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
1261 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
1321 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
1381 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
1441 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
1501 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
1561 atataaatgt caggctccct tatacacagc cagtctgcag gtcgaccata gtgactggat
1621 atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat ttaatatatt
1681 gatatttata tcattttacg tttctcgttc aacttttcta tacaaagttg atagcttggc
1741 gtaatcatgg tcatagctgt ttcctgtgtg aagcttagct tgagcttgga tcagattgtc
1801 gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta
1861 agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa ggtttatccg
1921 ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga
1981 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
2041 aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct
2101 ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga
2161 gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca
2221 ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc
2281 tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg
2341 accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca
2401 cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc
2461 tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg
2521 ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg
2581 ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg
2641 cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc
2701 ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca
2761 ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg
2821 cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga
2881 cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt
2941 cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga
3001 tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct
3061 aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat
3121 gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac
3181 cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc
3241 gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg
3301 gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc
3361 gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg
3421 gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc
3481 ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc
3541 acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc
3601 ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc
3661 acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa
3721 cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc
3781 atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc
3841 gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca
3901 tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg
3961 tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt
4021 aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa
4081 aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt
4141 tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg aacccccaag
4201 cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg gcgcggcgct
4261 gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc aacgcatcga
4321 ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc gcaaagaatc
4381 ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg gcgacgagca
4441 accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc gcagcatcat
4501 ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg tgatccgcta
4561 cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg ccagtgtgtg
4621 ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga accgataccg
4681 ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg acgtactcaa
4741 gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa cctgcattcg
4801 gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg gccgcctggt
4861 gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga gcgaaaccgg
4921 gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga tcacagaagg
4981 caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc ccggcatcgg
5041 ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca gatggttgtt
5101 caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct gtttcaccgt
5161 gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg aggcggggca
5221 ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag catccgccgg
5281 ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa aaggtcgaaa
5341 aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca ttgggaaccg
5401 gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca tgtaagtgac
5461 tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac ttattaaaac
5521 tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg aagagctgca
5581 aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc gtcggcctat
5641 cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac cagggcgcgg
5701 acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc tgcctcgcgc
5761 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
5821 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
5881 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
5941 ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca
6001 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
6061 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
6121 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
6181 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
6241 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
6301 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
6361 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
6421 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
6481 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
6541 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
6601 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
6661 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
6721 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
6781 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
6841 tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat ctcccaattt
6901 gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct gatagtttgg
6961 ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg cgaagcggcg
7021 tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg atctcgcctt
7081 tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga tcttcttctt
7141 gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc ggcaggcgct
7201 ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact gcgctgtacc
7261 aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg ggcggcgagt
7321 tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga accggatcaa
7381 agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct tttgtcagca
7441 agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga atgtcattgc
7501 gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga atgatgtcgt
7561 cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca ggggaagccg
7621 aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc cttacggtca
7681 ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact gcggagccgt
7741 acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca actacctctg
7801 atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac tttgttttag
7861 ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
7921 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa aaacatgtca
7981 taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc ggtcaaggtt
8041 ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga accgaacgag
8101 gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc tgtcatcgtt
8161 acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca gcttagttgc
8221 cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa cggctctccc
8281 gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg tgccgagctg
8341 ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa acaaattgac
8401 gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta acgccgaatt
8461 gaattatcag cttgcatgcc ggtcgatcta gtaacataga tgacaccgcg cgcgataatt
8521 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
8581 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
8641 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca
8701 atcttaagaa actttattgc caaatgtttg aacgatctgc ttgactctag ctagagtccg
8761 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
8821 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
8881 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
8941 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
9001 catcgccgtg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg agcctggcga
9061 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
9121 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
9181 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
9241 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
9301 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
9361 ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg gacaggtcgg
9421 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
9481 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
9541 aacctgcgtg caatccatct tgttcaatca tgcctcgatc gagttgagag tgaatatgag
9601 actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt tgacaagaaa
9661 tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg gtttctgacg
9721 tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt caacgttgcg
9781 gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg gtcataacgt
9841 gactccctta attctcatgt atgataattc gagggtaccg gtatcgcagc ccccttttgt
9901 ctcccttttg tctccctttt gtctcccttt tgtctccctt ttgtctccct tttgtctccc
9961 ttttgtctcc ctgggcaggc ctcgataagc ttgatatcga attaattcct gcagccccgc
10021 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggtatttt tacaacaatt
10081 accaacaaca acaaacaaca aacaacacaa gtttgtacaa aaaagcaggc tttaaaggaa
10141 ccaattcagt cgactggatc catggtgtct aagggcgaag agctgatcaa ggaaaatatg
10201 cgtatgaagg tggtcatgga aggttcggtc aacggccacc aattcaaatg cacaggtgaa
10261 ggagaaggca gaccgtacga gggagtgcaa accatgagga tcaaagtcat cgagggagga
10321 cccctgccat ttgcctttga cattcttgcc acgtcgttca tgtatggcag ccgtaccttt
10381 atcaagtacc cggccgacat ccctgatttc tttaaacagt cctttcctga gggttttact
10441 tgggaaagag ttacgagata cgaagatggt ggagtcgtca ccgtcacgca ggacaccagc
10501 cttgaggatg gcgagctcgt ctacaacgtc aaggtcagag gggtaaactt tccctccaat
10561 ggtcccgtga tgcagaagaa gaccaagggt tgggagccta atacagagat gatgtatcca
10621 gcagatggtg gtctgagagg atacactgac atcgcactga aagttgatgg tggtggccat
10681 ctgcactgca acttcgtgac aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg
10741 cccggtgtcc atgccgttga tcaccgcctg gaaaggatcg aggagagtga caatgaaacc
10801 tacgtagtgc aaagagaagt ggcagttgcc aaatacagca accttggtgg tggcatggac
10861 gagctgtaca agtccggact cagatctgga ggctccggag gcccagagcc agcgaagtct
10921 gctcccgccc cgaaaaaggg ctccaagaag gcggtgacta aggcgcagaa gaaaggcggc
10981 aagaagcgca agcgcagccg caaggagagc tattccatct atgtgtacaa ggttctgaag
11041 caggtccacc ctgacaccgg catttcgtcc aaggccatgg gcatcatgaa ttcgtttgtg
11101 aacgacattt tcgagcgcat cgcaggtgag gcttcccgcc tggcgcatta caacaagcgc
11161 tcgaccatca cctccaggga gatccagacg gccgtgcgcc tgctgctgcc tggggagttg
11221 gccaagcacg ccgtgtccga gggtactaag gccatcacca agtacaccag cgctaagtaa
11281 gcggccgcac tcgagatatc tagacccagc tttcttgtac aaagtggcct aactctgtgg
11341 tctcagcttg ctctcaagat caaaggctta aaaagctggg gttttatgaa tgggatcaaa
11401 gtttcttttt ttcttttata tttgcttctc catttgtttg tttcatttcc ctttttgttt
11461 tcgtttctat gatgcacttg tgtgtgacaa actctctggg tttttactta cgtctgcgtt
11521 tcaaaaaaaa aaaccgcttt cgttttgcgt tttagtccca ttgttttgta gctctgagtg
11581 atcgaattga tgcctcttta ttccttttgt tccctataat ttctttcaaa actcagaaga
11641 aaaaccttga aactctttgc aatgttaata taagtattgt ataagatttt tattgatttg
11701 gttattagtc ttacttttgc tacctccatc ttcacttgga actgatattc tgaatagtta
11761 aagcgttaca tgtgttccat tcacaaatga acttaaacta gcacaaagtc agatatttta
11821 agatcgcacc atttcgcttg agggtacccg gggatcctct agagggcc
//
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