pCMV-hA3A-BE3-C106S

LOCUS       pCMV-hA3A-BE3-C106S               8442 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Expresses hA3A-BE3-C106S in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-hA3A-BE3-C106S
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Wang X, Li J, Wang Y, Yang V, Wei J, Wu J, Wang R, Huang X, Chen J, 
            Yang L
  TITLE     Efficient base editing in methylated regions with a human 
            APOBEC3A-Cas9 fusion.
  JOURNAL   Nat Biotechnol.,  2018
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        167..546
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        547..750
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     701..721
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     792..811
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        792..810
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      818..827
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             824..1420
                     /codon_start=1
                     /gene="human APOBEC3A"
                     /gene_synonym="A3A"
                     /product="human cytidine deaminase (C to U editing enzyme) 
                     that efficiently edits methylated cytosines (Wang et al., 
                     2018)"
                     /label=APOBEC3A
                     /note="also known as hA3A"
                     /translation="MEASPASGPRHLMDPHIFTSNFNNGIGRHKTYLCYEVERLDNGTS
                     VKMDQHRGFLHNQAKNLLCGFYGRHAELRFLDLVPSLQLDPAQIYRVTWFISWSPCFSW
                     GSAGEVRAFLQENTHVRLRIFAARIYDYDPLYKEALQMLRDAGAQVSIMTYDEFKHCWD
                     TFVDHQGCPFQPWDGLDEHSQALSGRLRAILQNQGN"
     CDS             1469..5569
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5582..5830
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5843..5863
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5872..5889
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5912..5929)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5918..6142
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6213..6229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6213..6229)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6226..6248)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6237..6253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6261..6291)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6306..6327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6444..6461)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6615..7203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6695..6714)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7374..8234)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7997..8016
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8235..8339)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8418..8437)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
       61 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
      121 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
      181 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
      241 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
      301 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
      361 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
      421 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
      481 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
      541 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
      601 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
      661 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
      721 gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat ccgctagaga
      781 tccgcggccg ctaatacgac tcactatagg gagagccgcc accatggaag ccagcccagc
      841 atccgggccc agacacttga tggatccaca catattcact tccaacttta acaatggcat
      901 tggaaggcat aagacctacc tgtgctacga agtggagcgc ctggacaatg gcacctcggt
      961 caagatggac cagcacaggg gctttctaca caaccaggct aagaatcttc tctgtggctt
     1021 ttacggccgc catgcggagc tgcgcttctt ggacctggtt ccttctttgc agttggaccc
     1081 ggcccagatc tacagggtca cttggttcat ctcctggagc ccctgcttct cctggggcag
     1141 tgccggggaa gtgcgtgcgt tccttcagga gaacacacac gtgagactgc gtatcttcgc
     1201 tgcccgcatc tatgattacg accccctata taaggaggca ctgcaaatgc tgcgggatgc
     1261 tggggcccaa gtctccatca tgacctacga tgaatttaag cactgctggg acacctttgt
     1321 ggaccaccag ggatgtccct tccagccctg ggatggacta gatgagcaca gccaagccct
     1381 gagtgggagg ctgcgggcca ttctccagaa tcagggaaac agcggcagcg agactcccgg
     1441 gacctcagag tccgccacac ccgaaagtga taaaaagtat tctattggtt tagccatcgg
     1501 cactaattcc gttggatggg ctgtcataac cgatgaatac aaagtacctt caaagaaatt
     1561 taaggtgttg gggaacacag accgtcattc gattaaaaag aatcttatcg gtgccctcct
     1621 attcgatagt ggcgaaacgg cagaggcgac tcgcctgaaa cgaaccgctc ggagaaggta
     1681 tacacgtcgc aagaaccgaa tatgttactt acaagaaatt tttagcaatg agatggccaa
     1741 agttgacgat tctttctttc accgtttgga agagtccttc cttgtcgaag aggacaagaa
     1801 acatgaacgg caccccatct ttggaaacat agtagatgag gtggcatatc atgaaaagta
     1861 cccaacgatt tatcacctca gaaaaaagct agttgactca actgataaag cggacctgag
     1921 gttaatctac ttggctcttg cccatatgat aaagttccgt gggcactttc tcattgaggg
     1981 tgatctaaat ccggacaact cggatgtcga caaactgttc atccagttag tacaaaccta
     2041 taatcagttg tttgaagaga accctataaa tgcaagtggc gtggatgcga aggctattct
     2101 tagcgcccgc ctctctaaat cccgacggct agaaaacctg atcgcacaat tacccggaga
     2161 gaagaaaaat gggttgttcg gtaaccttat agcgctctca ctaggcctga caccaaattt
     2221 taagtcgaac ttcgacttag ctgaagatgc caaattgcag cttagtaagg acacgtacga
     2281 tgacgatctc gacaatctac tggcacaaat tggagatcag tatgcggact tatttttggc
     2341 tgccaaaaac cttagcgatg caatcctcct atctgacata ctgagagtta atactgagat
     2401 taccaaggcg ccgttatccg cttcaatgat caaaaggtac gatgaacatc accaagactt
     2461 gacacttctc aaggccctag tccgtcagca actgcctgag aaatataagg aaatattctt
     2521 tgatcagtcg aaaaacgggt acgcaggtta tattgacggc ggagcgagtc aagaggaatt
     2581 ctacaagttt atcaaaccca tattagagaa gatggatggg acggaagagt tgcttgtaaa
     2641 actcaatcgc gaagatctac tgcgaaagca gcggactttc gacaacggta gcattccaca
     2701 tcaaatccac ttaggcgaat tgcatgctat acttagaagg caggaggatt tttatccgtt
     2761 cctcaaagac aatcgtgaaa agattgagaa aatcctaacc tttcgcatac cttactatgt
     2821 gggacccctg gcccgaggga actctcggtt cgcatggatg acaagaaagt ccgaagaaac
     2881 gattactcca tggaattttg aggaagttgt cgataaaggt gcgtcagctc aatcgttcat
     2941 cgagaggatg accaactttg acaagaattt accgaacgaa aaagtattgc ctaagcacag
     3001 tttactttac gagtatttca cagtgtacaa tgaactcacg aaagttaagt atgtcactga
     3061 gggcatgcgt aaacccgcct ttctaagcgg agaacagaag aaagcaatag tagatctgtt
     3121 attcaagacc aaccgcaaag tgacagttaa gcaattgaaa gaggactact ttaagaaaat
     3181 tgaatgcttc gattctgtcg agatctccgg ggtagaagat cgatttaatg cgtcacttgg
     3241 tacgtatcat gacctcctaa agataattaa agataaggac ttcctggata acgaagagaa
     3301 tgaagatatc ttagaagata tagtgttgac tcttaccctc tttgaagatc gggaaatgat
     3361 tgaggaaaga ctaaaaacat acgctcacct gttcgacgat aaggttatga aacagttaaa
     3421 gaggcgtcgc tatacgggct ggggacgatt gtcgcggaaa cttatcaacg ggataagaga
     3481 caagcaaagt ggtaaaacta ttctcgattt tctaaagagc gacggcttcg ccaataggaa
     3541 ctttatgcag ctgatccatg atgactcttt aaccttcaaa gaggatatac aaaaggcaca
     3601 ggtttccgga caaggggact cattgcacga acatattgcg aatcttgctg gttcgccagc
     3661 catcaaaaag ggcatactcc agacagtcaa agtagtggat gagctagtta aggtcatggg
     3721 acgtcacaaa ccggaaaaca ttgtaatcga gatggcacgc gaaaatcaaa cgactcagaa
     3781 ggggcaaaaa aacagtcgag agcggatgaa gagaatagaa gagggtatta aagaactggg
     3841 cagccagatc ttaaaggagc atcctgtgga aaatacccaa ttgcagaacg agaaacttta
     3901 cctctattac ctacaaaatg gaagggacat gtatgttgat caggaactgg acataaaccg
     3961 tttatctgat tacgacgtcg atcacattgt accccaatcc tttttgaagg acgattcaat
     4021 cgacaataaa gtgcttacac gctcggataa gaaccgaggg aaaagtgaca atgttccaag
     4081 cgaggaagtc gtaaagaaaa tgaagaacta ttggcggcag ctcctaaatg cgaaactgat
     4141 aacgcaaaga aagttcgata acttaactaa agctgagagg ggtggcttgt ctgaacttga
     4201 caaggccgga tttattaaac gtcagctcgt ggaaacccgc caaatcacaa agcatgttgc
     4261 acagatacta gattcccgaa tgaatacgaa atacgacgag aacgataagc tgattcggga
     4321 agtcaaagta atcactttaa agtcaaaatt ggtgtcggac ttcagaaagg attttcaatt
     4381 ctataaagtt agggagataa ataactacca ccatgcgcac gacgcttatc ttaatgccgt
     4441 cgtagggacc gcactcatta agaaataccc gaagctagaa agtgagtttg tgtatggtga
     4501 ttacaaagtt tatgacgtcc gtaagatgat cgcgaaaagc gaacaggaga taggcaaggc
     4561 tacagccaaa tacttctttt attctaacat tatgaatttc tttaagacgg aaatcactct
     4621 ggcaaacgga gagatacgca aacgaccttt aattgaaacc aatggggaga caggtgaaat
     4681 cgtatgggat aagggccggg acttcgcgac ggtgagaaaa gttttgtcca tgccccaagt
     4741 caacatagta aagaaaactg aggtgcagac cggagggttt tcaaaggaat cgattcttcc
     4801 aaaaaggaat agtgataagc tcatcgctcg taaaaaggac tgggacccga aaaagtacgg
     4861 tggcttcgat agccctacag ttgcctattc tgtcctagta gtggcaaaag ttgagaaggg
     4921 aaaatccaag aaactgaagt cagtcaaaga attattgggg ataacgatta tggagcgctc
     4981 gtcttttgaa aagaacccca tcgacttcct tgaggcgaaa ggttacaagg aagtaaaaaa
     5041 ggatctcata attaaactac caaagtatag tctgtttgag ttagaaaatg gccgaaaacg
     5101 gatgttggct agcgccggag agcttcaaaa ggggaacgaa ctcgcactac cgtctaaata
     5161 cgtgaatttc ctgtatttag cgtcccatta cgagaagttg aaaggttcac ctgaagataa
     5221 cgaacagaag caactttttg ttgagcagca caaacattat ctcgacgaaa tcatagagca
     5281 aatttcggaa ttcagtaaga gagtcatcct agctgatgcc aatctggaca aagtattaag
     5341 cgcatacaac aagcacaggg ataaacccat acgtgagcag gcggaaaata ttatccattt
     5401 gtttactctt accaacctcg gcgctccagc cgcattcaag tattttgaca caacgataga
     5461 tcgcaaacga tacacttcta ccaaggaggt gctagacgcg acactgattc accaatccat
     5521 cacgggatta tatgaaactc ggatagattt gtcacagctt gggggtgact ctggtggttc
     5581 tactaatctg tcagatatta ttgaaaagga gaccggtaag caactggtta tccaggaatc
     5641 catcctcatg ctcccagagg aggtggaaga agtcattggg aacaagccgg aaagcgatat
     5701 actcgtgcac accgcctacg acgagagcac cgacgagaat gtcatgcttc tgactagcga
     5761 cgcccctgaa tacaagcctt gggctctggt catacaggat agcaacggtg agaacaagat
     5821 taagatgctc tctggtggtt ctcccaagaa gaagaggaaa gtctaaccgg tcatcatcac
     5881 catcaccatt gagtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
     5941 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     6001 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     6061 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     6121 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgataccg
     6181 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     6241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctagggt
     6301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     6361 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     6421 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6481 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6541 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6601 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6661 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6721 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6781 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6841 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6901 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6961 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7021 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     7081 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7141 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7201 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7261 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7321 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7381 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7441 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7501 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7561 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7621 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7681 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7741 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7801 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7861 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7921 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7981 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8041 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8101 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8161 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8221 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8281 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8341 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc tcccgatccc
     8401 ctagggtcga ctctcagtac aatctgctct gatgccgcat ag
//