pAAVLINK-BRCA2-B

LOCUS       pAAVLINK-BRCA2-B               7300 bp DNA     circular SYN 22-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7300
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..141
                     /label=AAV2 ITR
     protein_bind    156..189
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          190..281
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             282..4456
                     /codon_start=1
                     /label=BRCA2
                     /translation="GLLFCSWHKTECFY*SSAKSCETV**Y*EY**GNFCRGTSNKFIF
                     K*MS*FCCFNV*DRKS***NCK*KK**MPTDITK*Y*NDYWHFC*RNY*KLQEKY*K*R
                     *QIYCCQ*KFS*LRI*WQ*FK*K*YCLYS*R*NGLAIY*SAQHMS*IIWPVYEGGKHSD
                     *RRFVRFNFFGSCESSRSMSW*YFK*RTVNCY*NGAKYKRF*DF*YIFSDCKWEKY*CR
                     QRVI**NCKFL*SETRRIA*LFLKF*ITF*HKKEQNGHSKL*GNRHS*TQNTERKCPSW
                     YWKSTSDLPGTTRT**KDQRTYSVGFSYS*REKS*NCKGIFGQSEKPF**KRARY**NH
                     QF*PSMGKDPKVQRGL*RP*ISM*DH*DHSCPKV*RNAEFSQ***KPCFY*DCGAT*AL
                     K**FM*TN*KSQNIKKYLFES*ST*KCRKRNSKKSCNLLHKSVPLFSH*KFSLSFLHKL
                     **KNFCESDFIT*SKKMA*RRNI*WSTRKNKYCRLCRKLFV*K*FKQYYS*K*QKSSLR
                     KTRYLFK*Q*HV*QLFLPF**GI**FRISLKK*T*FWY*ASIEEC*RSKKH*FFQSNIQ
                     CKRCKCIPTNCK*RYLR*GTCD*LFTLQK*KCSH*IVHI****F*GRATCI*DSQW*NR
                     LCFT*NN*KSERHIYRQFQ*SN*GKQRE*IKNLPNENYGRLLRGIG*FRGYSS*LSR**
                     *M*HAFT*GFC*HSE*RNFTT*PKYVWIGESF*NITL*C*FGNFRYM*M*YREAS*VSL
                     ICKYLWDF*HSKWKICPGIRCFITKRKTSVF*NRR*YQASLFQSIV*K*RTFRPAHKRR
                     KYCYTYSRTFNIPKRLFI*CGKFICFLWI*YSKWKASFHFRKFLTQS*GSVRGI*FNQN
                     *A*SSLFTYV*TKCIKNTSSC**EKPRALCKLRNGKNLQ*RI*IIK*LKC*RWFFRK*S
                     LY*SFSISLSISTRQTTVGIRNQSLTC*EHSCFGKRTGFT*KRKNGNW*N*NFF*CSCE
                     NKYRSLFYLLQRFRKLL*NRSSRNC*SFYGR**TDRF*TAKSCHTFSFYMSRK*GNGFV
                     KFKNWKKKRRAPYLSGRTLNQKKLIK*I*QDNRKSRKILKGFKKHSRWHNKRSKIVYAS
                     CFFRADYLCTLSHN*GTSRDTESKFYRTWSRISV*ISFV*TSDFGKIFKQFSSFRTSIL
                     SSFCYKK*KNETLDYYRQTNQSLCSTF*N*ITFSQS*TVC*EY*LGGKQTKAKH*WTWL
                     ****K*D**Q*DSSV*QKQLQSSSSCNFHKV*RRTFRFNYKSSECQRYTGYAN*EETKA
                     TRLSTARQSVSCKNIHSASNLSESSSRRPSSLCVFS*TAVYVWRF*TLHKN*QQKCRVF
                     SVSH*RLFW*GKFMDWKRNTVG*WRMAHTLQ*WKGWKRRIL*GSV*HS"
     misc_feature    752..757
                     /label=SDS
     misc_feature    4212..4217
                     /label=SDS
     intron          4457..4497
                     /label=Splicing Donor
     misc_feature    4501..4534
                     /label=lox2272-JT1571
     repeat_region   4563..4703
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4563..4692
                     /label=AAV2 ITR
     rep_origin      4778..5233
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5515..5619
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5620..6480
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6651..7239
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtataac ttcgtatagc atacattata
      181 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
      241 tattggtctt actgacatcc actttgcctt tctctccaca ggggctttta ttctgctcat
      301 ggcacaaaac tgaatgtttc tactgaagct ctgcaaaaag ctgtgaaact gtttagtgat
      361 attgagaata ttagtgagga aacttctgca gaggtacatc caataagttt atcttcaagt
      421 aaatgtcatg attctgttgt ttcaatgttt aagatagaaa atcataatga taaaactgta
      481 agtgaaaaaa ataataaatg ccaactgata ttacaaaata atattgaaat gactactggc
      541 acttttgttg aagaaattac tgaaaattac aagagaaata ctgaaaatga agataacaaa
      601 tatactgctg ccagtagaaa ttctcataac ttagaatttg atggcagtga ttcaagtaaa
      661 aatgatactg tttgtattca taaagatgaa acggacttgc tatttactga tcagcacaac
      721 atatgtctta aattatctgg ccagtttatg aaggagggaa acactcagat taaagaagat
      781 ttgtcagatt taactttttt ggaagttgcg aaagctcaag aagcatgtca tggtaatact
      841 tcaaataaag aacagttaac tgctactaaa acggagcaaa atataaaaga ttttgagact
      901 tctgatacat tttttcagac tgcaagtggg aaaaatatta gtgtcgccaa agagtcattt
      961 aataaaattg taaatttctt tgatcagaaa ccagaagaat tgcataactt ttccttaaat
     1021 tctgaattac attctgacat aagaaagaac aaaatggaca ttctaagtta tgaggaaaca
     1081 gacatagtta aacacaaaat actgaaagaa agtgtcccag ttggtactgg aaatcaacta
     1141 gtgaccttcc agggacaacc cgaacgtgat gaaaagatca aagaacctac tctgttgggt
     1201 tttcatacag ctagcgggaa aaaagttaaa attgcaaagg aatctttgga caaagtgaaa
     1261 aacctttttg atgaaaaaga gcaaggtact agtgaaatca ccagttttag ccatcaatgg
     1321 gcaaagaccc taaagtacag agaggcctgt aaagaccttg aattagcatg tgagaccatt
     1381 gagatcacag ctgccccaaa gtgtaaagaa atgcagaatt ctctcaataa tgataaaaac
     1441 cttgtttcta ttgagactgt ggtgccacct aagctcttaa gtgataattt atgtagacaa
     1501 actgaaaatc tcaaaacatc aaaaagtatc tttttgaaag ttaaagtaca tgaaaatgta
     1561 gaaaaagaaa cagcaaaaag tcctgcaact tgttacacaa atcagtcccc ttattcagtc
     1621 attgaaaatt cagccttagc tttttacaca agttgtagta gaaaaacttc tgtgagtcag
     1681 acttcattac ttgaagcaaa aaaatggctt agagaaggaa tatttgatgg tcaaccagaa
     1741 agaataaata ctgcagatta tgtaggaaat tatttgtatg aaaataattc aaacagtact
     1801 atagctgaaa atgacaaaaa tcatctctcc gaaaaacaag atacttattt aagtaacagt
     1861 agcatgtcta acagctattc ctaccattct gatgaggtat ataatgattc aggatatctc
     1921 tcaaaaaata aacttgattc tggtattgag ccagtattga agaatgttga agatcaaaaa
     1981 aacactagtt tttccaaagt aatatccaat gtaaaagatg caaatgcata cccacaaact
     2041 gtaaatgaag atatttgcgt tgaggaactt gtgactagct cttcaccctg caaaaataaa
     2101 aatgcagcca ttaaattgtc catatctaat agtaataatt ttgaggtagg gccacctgca
     2161 tttaggatag ccagtggtaa aatcgtttgt gtttcacatg aaacaattaa aaaagtgaaa
     2221 gacatattta cagacagttt cagtaaagta attaaggaaa acaacgagaa taaatcaaaa
     2281 atttgccaaa cgaaaattat ggcaggttgt tacgaggcat tggatgattc agaggatatt
     2341 cttcataact ctctagataa tgatgaatgt agcacgcatt cacataaggt ttttgctgac
     2401 attcagagtg aagaaatttt acaacataac caaaatatgt ctggattgga gaaagtttct
     2461 aaaatatcac cttgtgatgt tagtttggaa acttcagata tatgtaaatg tagtataggg
     2521 aagcttcata agtcagtctc atctgcaaat acttgtggga tttttagcac agcaagtgga
     2581 aaatctgtcc aggtatcaga tgcttcatta caaaacgcaa gacaagtgtt ttctgaaata
     2641 gaagatagta ccaagcaagt cttttccaaa gtattgttta aaagtaacga acattcagac
     2701 cagctcacaa gagaagaaaa tactgctata cgtactccag aacatttaat atcccaaaaa
     2761 ggcttttcat ataatgtggt aaattcatct gctttctctg gatttagtac agcaagtgga
     2821 aagcaagttt ccattttaga aagttcctta cacaaagtta agggagtgtt agaggaattt
     2881 gatttaatca gaactgagca tagtcttcac tattcaccta cgtctagaca aaatgtatca
     2941 aaaatacttc ctcgtgttga taagagaaac ccagagcact gtgtaaactc agaaatggaa
     3001 aaaacctgca gtaaagaatt taaattatca aataacttaa atgttgaagg tggttcttca
     3061 gaaaataatc actctattaa agtttctcca tatctctctc aatttcaaca agacaaacaa
     3121 cagttggtat taggaaccaa agtctcactt gttgagaaca ttcatgtttt gggaaaagaa
     3181 caggcttcac ctaaaaacgt aaaaatggaa attggtaaaa ctgaaacttt ttctgatgtt
     3241 cctgtgaaaa caaatataga agtttgttct acttactcca aagattcaga aaactacttt
     3301 gaaacagaag cagtagaaat tgctaaagct tttatggaag atgatgaact gacagattct
     3361 aaactgccaa gtcatgccac acattctctt tttacatgtc ccgaaaatga ggaaatggtt
     3421 ttgtcaaatt caagaattgg aaaaagaaga ggagagcccc ttatcttagt gggagaaccc
     3481 tcaatcaaaa gaaacttatt aaatgaattt gacaggataa tagaaaatca agaaaaatcc
     3541 ttaaaggctt caaaaagcac tccagatggc acaataaaag atcgaagatt gtttatgcat
     3601 catgtttctt tagagccgat tacctgtgta ccctttcgca caactaagga acgtcaagag
     3661 atacagaatc caaattttac cgcacctggt caagaatttc tgtctaaatc tcatttgtat
     3721 gaacatctga ctttggaaaa atcttcaagc aatttagcag tttcaggaca tccattttat
     3781 caagtttctg ctacaagaaa tgaaaaaatg agacacttga ttactacagg cagaccaacc
     3841 aaagtctttg ttccaccttt taaaactaaa tcacattttc acagagttga acagtgtgtt
     3901 aggaatatta acttggagga aaacagacaa aagcaaaaca ttgatggaca tggctctgat
     3961 gatagtaaaa ataagattaa tgacaatgag attcatcagt ttaacaaaaa caactccaat
     4021 caagcagtag ctgtaacttt cacaaagtgt gaagaagaac ctttagattt aattacaagt
     4081 cttcagaatg ccagagatat acaggatatg cgaattaaga agaaacaaag gcaacgcgtc
     4141 tttccacagc caggcagtct gtatcttgca aaaacatcca ctctgcctcg aatctctctg
     4201 aaagcagcag taggaggcca agttccctct gcgtgttctc ataaacagct gtatacgtat
     4261 ggcgtttcta aacattgcat aaaaattaac agcaaaaatg cagagtcttt tcagtttcac
     4321 actgaagatt attttggtaa ggaaagttta tggactggaa aaggaataca gttggctgat
     4381 ggcggatggc tcataccctc caatgatgga aaggctggaa aagaagaatt ttatagggct
     4441 ctgtgtgaca ctccaggtaa gtatcaaggt tacaagacag gtttaaggag accaataagc
     4501 tattattcgt ataggatact ttatacgaag ttatgaattc cacgtgcgga ccgagcggcc
     4561 gcaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4621 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4681 gcgagcgcgc agctgcctgc aggggcgcct gatgcggtat tttctcctta cgcatctgtg
     4741 cggtatttca caccgcatac gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     4801 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccttagcg
     4861 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4921 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4981 aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5041 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5101 acactcaact ctatctcggg ctattctttt gatttataag ggattttgcc gatttcggtc
     5161 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5221 acgtttacaa ttttatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     5281 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     5341 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     5401 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     5461 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     5521 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     5581 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     5641 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     5701 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     5761 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5821 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5881 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5941 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     6001 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     6061 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     6121 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     6181 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     6241 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     6301 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     6361 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     6421 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     6481 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     6541 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     6601 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     6661 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     6721 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6781 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6841 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6901 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6961 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7021 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7081 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7141 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     7201 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     7261 ttacggttcc tggccttttg ctggcctttt gctcacatgt
//