pAAVLINKv1-pCALM1-Cre-3'-SBF1-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-3'-SBF1-FLAG             7076 bp DNA     circular SYN 25-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7076)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7076
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3789
                     /codon_start=2
                     /gene="SBF1"
                     /gene_synonym="CMT4B3; DENND7A; MTMR5"
                     /product="myotubularin-related protein 5 isoform 1"
                     /label=SBF1
                     /note="isoform 1 is encoded by transcript variant 1; 
                     myotubularin-related protein 5; DENN/MADD domain containing
                     7A; inactive phosphatidylinositol 3-phosphatase 5"
                     /db_xref="GI:239735519"
                     /db_xref="CCDS:CCDS14091.2"
                     /db_xref="GeneID:6305"
                     /db_xref="HGNC:HGNC:10542"
                     /db_xref="MIM:603560"
                     /protein_id="NP_002963.2"
                     /translation="ACCRDYQRLGLGTLSSSLSRAKSEPFRISPVNRMYAICRSYPGLL
                     IVPQSVQDNALQRVSRCYRQNRFPVVCWRSGRSKAVLLRSGGLHGKGVVGLFKAQNAPS
                     PGQSQADSSSLEQEKYLQAVVSSMPRYADASGRNTLSGFSSAHMGSHVPSPRARVTTLS
                     NPMAASASRRTAPRGKWGSVRTSGRSSGLGTDVGSRLAGRDALAPPQANGGPPDPGFLR
                     PQRAALYILGDKAQLKGVRSDPLQQWELVPIEVFEARQVKASFKKLLKACVPGCPAAEP
                     SPASFLRSLEDSEWLIQIHKLLQVSVLVVELLDSGSSVLVGLEDGWDITTQVVSLVQLL
                     SDPFYRTLEGFRLLVEKEWLSFGHRFSHRGAHTLAGQSSGFTPVFLQFLDCVHQVHLQF
                     PMEFEFSQFYLKFLGYHHVSRRFRTFLLDSDYERIELGLLYEEKGERRGQVPCRSVWEY
                     VDRLSKRTPVFHNYMYAPEDAEVLRPYSNVSNLKVWDFYTEETLAEGPPYDWELAQGPP
                     EPPEEERSDGGAPQSRRRVVWPCYDSCPRAQPDAISRLLEELQRLETELGQPAERWKDT
                     WDRVKAAQRLEGRPDGRGTPSSLLVSTAPHHRRSLGVYLQEGPVGSTLSLSLDSDQSSG
                     STTSGSRQAARRSTSTLYSQFQTAESENRSYEGTLYKKGAFMKPWKARWFVLDKTKHQL
                     RYYDHRVDTECKGVIDLAEVEAVAPGTPTMGAPKTVDEKAFFDVKTTRRVYNFCAQDVP
                     SAQQWVDRIQSCLSDA"
     regulatory      1976..1985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2333..2338
                     /label=SDS
     misc_feature    3276..3281
                     /label=SDS
     misc_feature    3329..3334
                     /label=SDS
     CDS             3796..3861
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3865..4296
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4009..4014
                     /label=SDS
     repeat_region   4339..4479
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4339..4468
                     /label=AAV2 ITR
     repeat_region   4340..4480
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4554..5009
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5291..5395
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5396..6256
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6427..7015
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggcttgctg tcgcgactac
     1501 cagcgcctcg gtctgggcac cctgagcagc agcctgagcc gggccaagtc tgagcccttc
     1561 cgcatttctc cggtcaaccg catgtatgcc atctgccgca gctacccagg gctgctgatc
     1621 gtgccccaga gtgtccagga caacgccctg cagcgcgtgt cccgctgcta ccgccagaac
     1681 cgcttccccg tggtctgctg gcgcagcggg cggtccaagg cggtgctgct gcgctctgga
     1741 ggcctgcatg gcaaaggtgt cgtcggcctc ttcaaggccc agaacgcacc ttctccaggc
     1801 cagtcccagg cggactcgag tagcctggag caggagaagt acctgcaggc tgtggtcagc
     1861 tccatgcccc gctacgccga cgcgtcggga cgcaacacgc ttagcggctt ctcctcagcc
     1921 cacatgggca gtcacgttcc cagccccaga gccagggtca ccacgctgtc caaccccatg
     1981 gcggcctcgg cctccagacg gaccgcaccc cgaggtaagt ggggcagtgt ccggaccagt
     2041 ggacgcagca gtggccttgg caccgatgtg ggctcccggc tagctggcag agacgcgctg
     2101 gccccacccc aggccaacgg gggccctccc gacccgggct tcctgcgtcc gcagcgagca
     2161 gccctctata tccttgggga caaagcccag ctcaagggtg tgcggtcaga ccccctgcag
     2221 cagtgggagc tggtgcccat tgaggtattc gaggcacggc aggtgaaggc tagcttcaag
     2281 aagctgctga aagcatgtgt cccaggctgc cccgctgctg agcccagccc agcctccttc
     2341 ctgcgctcac tggaggactc agagtggctg atccagatcc acaagctgct gcaggtgtct
     2401 gtgctggtgg tggagctcct ggattcaggc tcctccgtgc tggtgggcct ggaggatggc
     2461 tgggacatca ccacccaggt ggtatccttg gtgcagctgc tctcagaccc cttctaccgc
     2521 acgctggagg gctttcgcct gctggtggag aaggagtggc tgtccttcgg ccatcgcttc
     2581 agccaccgtg gagctcacac cctggccggg cagagcagcg gcttcacacc cgtcttcctg
     2641 cagttcctgg actgcgtaca ccaggtccac ctgcagttcc ccatggagtt tgagttcagc
     2701 cagttctacc tcaagttcct cggctaccac catgtgtccc gccgtttccg gaccttcctg
     2761 ctcgactctg actatgagcg cattgagctg gggctgctgt atgaggagaa gggggaacgc
     2821 aggggccagg tgccgtgcag gtctgtgtgg gagtatgtgg accggctgag caagaggacg
     2881 cctgtgttcc acaattacat gtatgcgccc gaggacgcag aggtcctgcg gccctacagc
     2941 aacgtgtcca acctgaaggt gtgggacttc tacactgagg agacgctggc cgagggccct
     3001 ccctatgact gggaactggc ccaggggccc cctgaacccc cagaggaaga acggtctgat
     3061 ggaggcgctc cccagagcag gcgccgcgtg gtgtggccct gttacgacag ctgcccgcgg
     3121 gcccagcctg acgccatctc acgcctgctg gaggagctgc agaggctgga gacagagttg
     3181 ggccaacccg ctgagcgctg gaaggacacc tgggaccggg tgaaggctgc acagcgcctc
     3241 gagggccggc cagacggccg tggcacccct agctccctcc ttgtgtccac cgcaccccac
     3301 caccgtcgct cgctgggtgt gtacctgcag gaggggcccg tgggctccac cctgagcctc
     3361 agcctggaca gcgaccagag tagtggctca accacatccg gctcccgtca ggctgcccgc
     3421 cgcagcacca gcaccctgta cagccagttc cagacagcag agagtgagaa caggtcctac
     3481 gagggcactc tgtacaagaa gggggccttc atgaagcctt ggaaggcccg ctggttcgtg
     3541 ctggacaaga ccaagcacca gctgcgctac tacgaccacc gtgtggacac agagtgcaag
     3601 ggtgtcatcg acttggcgga ggtggaggct gtggcacctg gcacgcccac tatgggtgcc
     3661 cctaagaccg tggacgagaa ggccttcttt gacgtgaaga caacgcgtcg cgtttacaac
     3721 ttctgtgccc aggacgtgcc ctcggcccag cagtgggtgg accggatcca gagctgcctg
     3781 tcggacgcct gtacagacta caaagaccat gacggtgatt ataaagatca tgacatcgac
     3841 tacaaggatg acgatgacaa gtaactcgag ataatcaacc tctggattac aaaatttgtg
     3901 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     3961 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4021 aatcctggtt agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4081 caggggctcg gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg
     4141 ctattgcttt atttgtaacc atctagcttt atttgtgaaa tttgtgatgc tattgcttta
     4201 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     4261 tttcaggttc agggggagat gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg
     4321 tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4381 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4441 cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc
     4501 tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc
     4561 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4621 tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4681 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4741 acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc
     4801 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4861 gttccaaact ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat
     4921 tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4981 ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga
     5041 tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
     5101 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
     5161 tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct
     5221 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     5281 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     5341 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     5401 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     5461 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     5521 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     5581 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     5641 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     5701 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     5761 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     5821 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     5881 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     5941 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     6001 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     6061 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     6121 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     6181 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     6241 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     6301 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     6361 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     6421 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     6481 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     6541 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     6601 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     6661 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     6721 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     6781 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     6841 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     6901 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     6961 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7021 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//