| 质粒名称: | pET-hisM5RT |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002247 |
| 产品用途: | active thermostable RNase H minus reverse transcriptase |
| 存储实验室: | Po-Min Chiang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pET3c |
| 表达系统: | 细菌表达 |
| 启动子: | T7 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Neb stable |
|
插入基因信息 |
|
| 插入基因名称: | M-MuLV reverse transcriptase |
| 物种来源: | MMuLV |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimens.
Hsueh, Y.-W. & Chiang, P.-M.
PLOS Biol. 23, e3003537 (2025)
|
买家导航
pET-hisM5RT载体质粒基本信息
pET-hisM5RT质粒图谱载体图谱和pET-hisM5RT载体序列质粒序列多克隆位点信息
pET-hisM5RT质粒载体简介
pET-hisM5RT质粒序列载体序列
LOCUS pET-hisM5RT 6704 bp DNA circular SYN 17-APR-2026
DEFINITION Expresses his-tagged Mut5 reverse transcriptase in E. coli hosts.
ACCESSION .
VERSION .
KEYWORDS pET-hisM5RT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6704)
TITLE Bacterial expression plasmids for production of recombinant PNK
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 6704)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..6704
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 10..38
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
terminator complement(404..451)
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind 447..465
/label=T7 Term
/note="T7 terminator, reverse primer"
CDS complement(2589..2606)
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
RBS 2626..2648
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
primer_bind complement(2679..2698)
/label=T7
/note="T7 promoter, forward primer"
promoter complement(2680..2698)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(2782..2801)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS 4259..4450
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind complement(4444..4466)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 4552..4691
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 4607..4626
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4706..4723)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4877..5465)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4957..4976)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5636..6496)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6259..6278
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6497..6601)
/gene="bla"
/label=AmpR promoter
primer_bind 6669..6687
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
481 cagcttcctt tcgggctttg ttagcagccg gatccttaat taattaagcg ggaattgggt
541 gatgaatttt ctatgaggag ggtagaggtg tctggagtct ctgtgatggc tgcctttcgg
601 gccgcttggt cagccatccg gttgcctcta gcctcggcgc tgtgtccctt ttgatgtcct
661 ggacaatgga ttatgctaag tcttttgggc agaaagaggg cttttagtag ggccaagatc
721 tcgtctttat ttttgatctc tttgcctttt gatgtgagcc acccacgcct tctgtatatt
781 tctccatgga tatgggcagt agcaaaagca taacggctat tagtataaac atttagcttc
841 ttaccttctg ccatctttag ggcctgggtg agtgctatca gttcagcccg ctgagcggat
901 gtcccggctg gcagggcttt agcccagatt acctcggtct cggtggtcac cgcagctccc
961 gccttacgct gtccctcttg taagagactg cttccacccg tgtaccaggt gtggtcggcg
1021 tctgggagcg gctggtccgt taggtcgggt cgggttccgt gggcttcggc caggatatca
1081 aggcagttgt gttgcagccc ttcctcaggc agtgggagca gcgtagccgg gttcagggct
1141 accaccggtc cgaactggac ccggtccgtg tccaaaagca aggcctgata gtgagtcatc
1201 cgggcgttgg aaagccagcg gtcggggggt tgtttgacta gtgcctctac tgcatggggg
1261 gccagaatga ctagtggctg tcccatggtt agcttgcctg catcctttgt cagtacggca
1321 atggctgcta ccatccgtag gcaagggggc cacccagctg ctactgggtc tagctttttg
1381 gacaggtagg ccaccggccg acgccaaggt cccagttttt gcgttaggac acctttggcg
1441 tagccctgct tctcgtcgac aaagagttca aagggcttag tcaaatctgg caaccccagg
1501 gctggggcag ttagaagagc ttgcttgatt tcttgatagg ccttttgttg gtctgggccc
1561 caattaaaca gagtccccgg tttggtgaga gggtacaagg gggctgccat ttctgcaaac
1621 ccagggatcc agaggcgaca gaagcctgcc gtccctagga actcccttag ttgtcgaggg
1681 gtcttcggag taggctgccc catcacagtc tcttttctgg cctcagtcag ccatctatga
1741 ccctctttta gaagataccc cagatacttg acctgtttct ggcaaatttg ggctttcttg
1801 gccgaggccc gatacccgag gttccctagg gtttgtaaca gggcccgagt accttgttgg
1861 cagtctagct cagaagtggc ggccagcagt aagtcatcca cgtactgtag caggatcaag
1921 tctgggtgct ggatccggaa gtctgctagg tctctgtgca gtgcctcatt aaacagggtg
1981 ggactgtttt tgaaaccctg tgggagtctg gtccaggtca attgtcctga gattcccatc
2041 tctggatctc tccactcaaa ggcgaagaga ggctgactgg tggggtggag tctcaggcag
2101 aaaaaggcat cctttaaatc aagcacagtg taccactggt gggacggtgg gggcccgctc
2161 aagaggttgt aagggttggg cacggtgggg tggatgtctt ccacccgctt gttgacttct
2221 ctcagatcct ggacaggcct ataatcatta gtccctggtt tcttaacggg tagcaggggc
2281 gtgttccagg gggactggca gggtaccagt attccctggt ccaacagtct ctgtatgtgg
2341 ggcttgatcc ccagtctggc ttcttgtgac atggggtatt gttttatgga cacgggggta
2401 gaggttgctt tcagaggtat gatcagagga gcttggcgaa ctgccagtcc catgcccccg
2461 gtttccgccc aggcctgagg aaaatcagac agccatgtgg accctagaga aacatctggc
2521 tcttttgagg tctcatgtag ccgatgctca tcttctatat ttagggtcat gcccatggaa
2581 ccagatccat gatgatggtg gtggtgcgat cctctcatat gtatatctcc ttcttaaagt
2641 taaacaaaat tatttctaga gggaaaccgt tgtggtctcc ctatagtgag tcgtattaat
2701 ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc atcaccggcg
2761 ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc
2821 gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg
2881 ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg gtgctcaacg
2941 gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgtcgac
3001 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
3061 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
3121 cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt
3181 cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca
3241 ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct
3301 acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg
3361 cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg
3421 accatcaggg acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg
3481 gaccgctgat cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat
3541 ggattgtagg cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga
3601 gccgggccac ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc
3661 aagaattgga gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca
3721 gaacatatcc atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt
3781 tgggtcctgg ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg
3841 gcggggttgc cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga
3901 ctgctgctgc aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt
3961 ttcgtaaagt ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat
4021 cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca
4081 ttgaccctga gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc
4141 acaacgttcc agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc
4201 tcgtttcatc ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt
4261 gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
4321 gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
4381 tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga
4441 aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
4501 gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
4561 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
4621 attgtactga gagtgcacca ttgcggtgtg aaataccgca cagatgcgta aggagaaaat
4681 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
4741 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
4801 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
4861 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
4921 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
4981 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
5041 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
5101 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
5161 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
5221 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
5281 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
5341 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
5401 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
5461 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
5521 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
5581 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
5641 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
5701 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
5761 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
5821 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
5881 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
5941 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
6001 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
6061 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
6121 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
6181 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
6241 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
6301 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
6361 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
6421 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
6481 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
6541 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
6601 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
6661 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//
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