| 质粒名称: | pGEX-2T-drG3BP1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000918 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-2T |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄青霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | G3BP1 |
| 物种来源: | zebrafish |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-2T-drG3BP1载体质粒基本信息
pGEX-2T-drG3BP1质粒图谱载体图谱和pGEX-2T-drG3BP1载体序列质粒序列多克隆位点信息
pGEX-2T-drG3BP1质粒载体简介
pGEX-2T-drG3BP1质粒序列载体序列
LOCUS pGEX-2T-drG3BP1 6384 bp DNA circular SYN 09-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6384)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 15, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6384
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..938
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 942..2375
/codon_start=1
/label=drG3BP1 Codon
/translation="MVMEKPSAQLVGREFVRQYYTLLNQAPDYLHRFYGKNSSYVHGGL
DNNGKPAEAVYGQSEIHKKVMALSFRDCHTKIRHVDAHATLNEGVVVQVLGGLSNNMQP
MRKFMQTFVLAPEGTVANKFYVHNDIFRYQDEVFGDSDSEPPEESEEDVEELERVHSPE
VVQEESSGYYEQTPCVEPEVPQEEVSVTPEPQPEPEVEVEPEPAAVELKAEPISQPEVH
VEEKTQRSPPSPTPADTAPTMPEDNRPSSWASVTSKNLPPGGVVPATGVPPHVVRVPSA
QPRVEVKTETQTTAQRPQRDQRPRDQRPGPSPAHRTPRPGVVREGESGESEVRRTVRYP
DSHQLFVGNVPHDVDKNELKEFFEQYGTVLELRINSGGKLPNFGFVVFDDSEPVQKILN
NRPIKLRGDVRLNVEEKKTRSAREGDRRDIRPRGPGGPRDRIGGSRGPPTRGGMAQKPS
FGAGRGTGPSEGRYTGPRQ"
promoter 2687..2791
/gene="bla"
/label=AmpR promoter
CDS 2792..3652
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3823..4411
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4655..4732
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 4733..5815
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 5828..5849
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5864..5894
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5902..5918
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5926..5942
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5938..6384
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(5958..5974)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatgaa aacctgtatt ttcagggatc catggttatg gaaaagccga
961 gtgcccagct ggttggccgt gaatttgttc gtcagtatta taccctgctg aatcaggccc
1021 cggattatct gcatcgcttt tatggcaaaa atagcagcta tgttcatggc ggtctggata
1081 ataatggcaa accggcagaa gcagtttatg gtcagagcga aattcataaa aaagtgatgg
1141 ccctgagttt tcgtgattgc cataccaaaa ttcgccatgt ggatgcacat gcaaccctga
1201 atgaaggcgt tgtggttcag gtgctgggcg gtctgagtaa taatatgcag ccgatgcgta
1261 aattcatgca gacctttgtg ctggccccgg aaggcaccgt tgccaataag ttttatgtgc
1321 ataatgatat cttccgctat caggatgaag tttttggtga cagtgatagc gaaccgccgg
1381 aagaaagtga agaagatgtt gaagaactgg aacgcgtgca tagcccggaa gtggtgcagg
1441 aagaaagcag cggctattat gaacagaccc cgtgcgttga accggaagtt ccgcaggaag
1501 aagtgagtgt taccccggaa ccgcagccgg aaccggaagt ggaagttgaa ccggagccgg
1561 cagcagttga actgaaagcc gaaccgatta gccagccgga agttcatgtt gaagaaaaaa
1621 cccagcgcag cccgccgagc ccgacccctg cagacacagc tccgaccatg ccggaagata
1681 atcgcccgag cagctgggcc agcgttacca gcaaaaatct gccgccgggc ggtgttgttc
1741 cggcaaccgg tgttccgccg catgttgttc gtgtgccgag tgcccaaccg cgtgttgaag
1801 ttaaaaccga aacccagacc accgcacagc gcccgcagcg tgatcagcgt ccgcgtgatc
1861 agcgcccggg tcctagccct gcccatcgta ccccgcgtcc gggtgttgtt cgcgaaggtg
1921 aaagcggcga aagcgaagtg cgccgtaccg tgcgctatcc ggatagtcat cagctgtttg
1981 ttggtaatgt tccgcatgat gtggataaaa atgaactgaa agaatttttc gagcagtatg
2041 gtaccgttct ggaactgcgc attaatagtg gcggtaaact gccgaatttt ggttttgtgg
2101 tttttgatga tagcgaacct gtgcagaaaa ttctgaataa tcgtccgatt aagctgcgtg
2161 gtgacgttcg tctgaatgtg gaagaaaaga aaacccgcag tgcccgtgaa ggtgaccgtc
2221 gcgatattcg tccgcgtggc ccgggtggtc cgcgtgaccg tattggtggc agtcgcggcc
2281 cgccgacccg tggtggtatg gcacagaaac cgagttttgg tgcaggccgt ggcaccggtc
2341 cgagtgaagg tcgttatacc ggtccgcgcc agtaagaatt catcgtgact gactgacgat
2401 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
2461 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
2521 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
2581 taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat
2641 aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
2701 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
2761 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
2821 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
2881 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
2941 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
3001 taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg
3061 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
3121 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
3181 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
3241 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
3301 cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa
3361 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
3421 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
3481 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
3541 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
3601 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
3661 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
3721 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
3781 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
3841 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
3901 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
3961 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
4021 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
4081 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
4141 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
4201 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
4261 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
4321 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
4381 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
4441 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
4501 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
4561 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
4621 gcatctgtgc ggtatttcac accgcataaa ttccgacacc atcgaatggt gcaaaacctt
4681 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc
4741 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
4801 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
4861 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
4921 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
4981 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
5041 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
5101 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
5161 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
5221 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
5281 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
5341 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
5401 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
5461 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
5521 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
5581 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
5641 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
5701 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
5761 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
5821 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
5881 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
5941 accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
6001 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
6061 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
6121 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
6181 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
6241 accaacgtaa cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
6301 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
6361 attatttttg atggcgttgg aatt
//
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