pLenti-EF1a-CDK6-mutPAM-PGK-puro

LOCUS      pLenti-EF1a-CDK6-mutPAM-PGK-puro              10558 bp ds-DNA     circular SYN 01-DEC-2022
DEFINITION  sg resistant CDK6 ORF.
ACCESSION   .
VERSION     .
KEYWORDS    pLenti-EF1a-CDK6-mutPAM-PGK-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10558)
  AUTHORS   Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, 
            Perrimon N, Signoretti S, Kaelin WG Jr
  TITLE     HIF-independent synthetic lethality between CDK4/6 inhibition and 
            VHL loss across species.
  JOURNAL   Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 
            10.1126/scisignal.aay0482.
  PUBMED    31575731
REFERENCE   2  (bases 1 to 10558)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 1, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10558
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     promoter        132..489
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     complement(155..175)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      340..475
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             524..1318
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(578..597)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1188..1207
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(1506..1525)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1550..1597
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      1926..2514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2415..2434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     enhancer        2689..2992
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2994..3192
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3147..3167
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3210..3390
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3437..3562
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4059..4292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4477..4521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4819..4936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5008..6186
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5239..6177
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6134..6154
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        6203..6221
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6295..6319
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7298..7322)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7369..7458
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     promoter        7500..7999
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7535..7558)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7932..7951
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8020..8619
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8020..8039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8516..8536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    8650..8683
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    8739..9327
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8792..8812)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9210..9221)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9330..9346)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9331..9347)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9536..9769
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9841..9962
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9878..9897)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9932..9951
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10002..10137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10158..10176)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(10199..10557)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10239..10260)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10451..10470
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
      181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      241 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      301 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
      361 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      421 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
      481 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
      541 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
      601 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
      661 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
      721 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
      781 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
      841 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
      901 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
      961 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     1021 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     1081 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     1141 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     1201 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     1261 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     1321 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     1381 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     1441 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     1501 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     1561 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     1621 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     1681 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     1741 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     1801 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1861 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1921 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1981 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2041 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     2101 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2161 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2221 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2281 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2341 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2401 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2461 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2521 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2581 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata
     2641 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     2701 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     2761 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     2821 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
     2881 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
     2941 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
     3001 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
     3061 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
     3121 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
     3181 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
     3241 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3301 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3361 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
     3421 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
     3481 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
     3541 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
     3601 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
     3661 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
     3721 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
     3781 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
     3841 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
     3901 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
     3961 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
     4021 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
     4081 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
     4141 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
     4201 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
     4261 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
     4321 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
     4381 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
     4441 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
     4501 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
     4561 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
     4621 tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac ccacctccca
     4681 accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga gagagacaga
     4741 gacagatcca ttcgattagt gaacggatcg gcactgcgtg cgccaattct gcagacaaat
     4801 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     4861 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     4921 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggttagtacc
     4981 gggcccgctc tagccctaga tttaaatggc tccggtgccc gtcagtgggc agagcgcaca
     5041 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
     5101 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
     5161 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
     5221 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
     5281 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
     5341 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     5401 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
     5461 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     5521 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     5581 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     5641 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     5701 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     5761 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     5821 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     5881 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     5941 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     6001 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
     6061 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     6121 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
     6181 tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa gctggctagc
     6241 cgcggaatct agactgccgg atcgaattgt cgacgaattc ggatctggcc aatcacaagt
     6301 ttgtacaaaa aagttggcat ggagaaggac ggcctgtgcc gcgctgacca gcagtacgaa
     6361 tgcgtggctg agatcgggga gggcgcctat gggaaggtgt tcaaggcccg cgacttgaag
     6421 aacggaggcc gtttcgtggc gttgaagcgc gtgcgggtgc agaccggcga ggagggcatg
     6481 ccgctctcca ccatccgcga ggtggcggtg ctgaggcacc tggagacctt cgagcacccc
     6541 aacgtggtca ggttgtttga tgtgtgcaca gtgtcacgaa cagacagaga aaccaaacta
     6601 actttagtgt ttgaacatgt cgatcaagac ttgaccactt acttggataa agttccagag
     6661 cctggagtgc ccactgaaac cataaaggat atgatgtttc agcttctccg aggtctggac
     6721 tttcttcatt cacaccgagt agtgcatcgc gatctaaaac cacagaacat tctggtgacc
     6781 agcagcggac aaataaaact cgctgacttc ggccttgccc gcatctatag tttccagatg
     6841 gctctaacct cagtggtcgt cacgctgtgg tacagagcac ccgaagtctt gctccagtcc
     6901 agctacgcca cccccgtgga tctctggagt gttggctgca tatttgcaga aatgtttcgt
     6961 agaaagcctc tttttcgtgg aagttcagat gttgatcaac taggaaaaat cttggacgtg
     7021 attggactcc caggagaaga agactggcct agagatgttg cccttcccag gcaggctttt
     7081 cattcaaaat ctgcccaacc aattgagaag tttgtaacag atatcgatga actaggcaaa
     7141 gacctacttc tgaagtgttt gacatttaac ccagccaaaa gaatatctgc ctacagtgcc
     7201 ctgtctcacc catacttcca ggacctggaa aggtgcaaag aaaacctgga ttcccacctg
     7261 ccgcccagcc agaacacctc ggagctgaat acagcctgac caactttctt gtacaaagtg
     7321 gtgggtggag gcggttcagg tggcggagga agcggaggcg gtggaagtta cccctacgac
     7381 gtgcccgact acgccggcta tccgtatgat gtcccggact atgcaggatc ctatccatac
     7441 gacgttccag attacgctta gttaattaag gccggccctc gaggttaacg aattctaccg
     7501 ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag ccccgctggg
     7561 cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca ccggtaggcg
     7621 ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc ccctagtcag
     7681 gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg aagtagcacg
     7741 tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg taggcctttg
     7801 gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc tgggaagggg
     7861 tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc gaaggtcctc
     7921 cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt tctcctcttc
     7981 ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta caagcccacg
     8041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     8101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     8161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     8221 gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     8281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     8341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     8401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     8461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     8521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     8581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgat gtacaagtag gaattcgtcg
     8641 agggacctaa taacttcgta tagcatacat tatacgaagt tatacatgtt taagggttcc
     8701 ggttccacta ggtacaattc gatatcaagc ttatcgataa tcaacctctg gattacaaaa
     8761 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8821 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8881 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8941 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     9001 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     9061 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     9121 tgttgtcggg gaaatcatcg tcctttcctt ggatgctcgc ctgtgttgcc acctggattc
     9181 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     9241 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     9301 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgatcg agacctagaa
     9361 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     9421 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc tttaagacca
     9481 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     9541 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     9601 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     9661 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     9721 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     9781 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     9841 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     9901 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     9961 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
    10021 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    10081 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
    10141 ggacgtaccc aattcgccct atagtgagtc gtattagcat gtgagcaaaa ggccagcata
    10201 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
    10261 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
    10321 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
    10381 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
    10441 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
    10501 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtc
//