| 质粒名称: | pmiRFP680-Actin |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000069 |
| 产品用途: | Expresses miRFP680 fused to N-terminus of human beta actin |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CMV
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a, TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | miRFP680, b-actin |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
pmiRFP680-Actin
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pmiRFP680-Actin载体质粒基本信息
pmiRFP680-Actin质粒图谱载体图谱和pmiRFP680-Actin载体序列质粒序列多克隆位点信息
pmiRFP680-Actin质粒载体简介
pmiRFP680-Actin质粒序列载体序列
LOCUS pmiRFP680-Actin 6081 bp DNA circular SYN 21-APR-2023
DEFINITION Vector for fusing photoactivatable mCherry to the N-terminus of a
partner protein.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6081)
AUTHORS Clontech
TITLE Direct Submission
JOURNAL Exported Jun 25, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6081
/lab_host="Mammalian Cells"
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 585..632
/label=0416-PM635-F
regulatory 607..616
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 613..1560
/codon_start=1
/label=miRFP680
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKDAGFI
GSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAETLESACAAAAQEVRKIT
GFDRVMIYRFASDFSGEVIAEDRCAEVESKLGLHYPASTVPAQARRLYTINPVRIIPDI
NYRPVPVTPDLNPVTGRPIDLSFAILRSVSPCHLEFMRNIGMHGTMSISILRGERLWGL
IVCHHRTPYYVDLDGRQACKRVAERLATQIGVMEE"
primer_bind complement(1539..1581)
/label=0416-PM635-R
CDS 1615..2736
/codon_start=1
/label=Actin
/translation="DDDIAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG
MGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL
TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVP
IYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDF
EQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSI
MKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWI
GGSILASLSTFQQMWISKQEYDESGPSIVHRKCF"
primer_bind 1737..1762
/label=0312-Actin-F
misc_feature 2740..2745
/label=MCS
/note="multiple cloning site of fluorescent protein
plasmids"
polyA_signal 2869..2990
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2960..2979)
/label=SV40-R-Universal
rep_origin complement(2997..3452)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3479..3583
/gene="bla"
/label=AmpR promoter
promoter 3585..3942
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3793..3928
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3977..4771
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin)"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
misc_feature 5003..5050
/label=HSV TK poly(A) signal
/note="herpesvirus thymidine kinase polyadenylation signal"
rep_origin 5379..5967
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
601 ccggtcgcca ccatggcgga aggatccgtc gccaggcagc ctgacctctt gacctgcgac
661 gatgagccga tccatatccc cggtgccatc caaccgcatg gactgctgct cgccctcgcc
721 gccgacatga cgatcgttgc cggcagcgac aaccttcccg aactcaccgg actggcgatc
781 ggcgccctga tcggccgctc tgcggccgat gtcttcgact cggagacgca caaccgtctg
841 acgatcgcct tggccgagcc cggggcggcc gtcggagcac cgatcactgt cggcttcacg
901 atgcgaaagg acgcaggctt catcggctcc tggcatcgcc atgatcagct catcttcctc
961 gagctcgagc ctccccagcg ggacgtcgcc gagccgcagg cgttcttccg ccgcaccaac
1021 agcgccatcc gccgccttca ggccgccgaa accttggaaa gcgcctgcgc cgccgcggcg
1081 caagaggtgc ggaagattac cggcttcgat cgggtgatga tctatcgctt cgcctccgac
1141 ttcagcggcg aagtgatcgc agaggatcgg tgcgccgagg tcgagtcaaa actaggcctg
1201 cactatcctg cctcaaccgt gccggcgcag gcccgtcggc tctataccat caacccggta
1261 cggatcattc ccgatatcaa ttatcggccg gtgccggtca ccccagacct caatccggtc
1321 accgggcggc cgattgatct tagcttcgcc atcctgcgca gcgtctcgcc ctgccatctg
1381 gagttcatgc gcaacatagg catgcacggc acgatgtcga tctcgatttt gcgcggcgag
1441 cgactgtggg gattgatcgt ttgccatcac cgaacgccgt actacgtcga tctcgatggc
1501 cgccaagcct gcaagagggt cgccgagagg ctggccactc agatcggcgt gatggaagag
1561 tccggactca gatctggcag cggtggaggc agcgcatccg gcggaagcgg aagcgatgat
1621 gatatcgccg cgctcgtcgt cgacaacggc tccggcatgt gcaaggccgg cttcgcgggc
1681 gacgatgccc cccgggccgt cttcccctcc atcgtggggc gccccaggca ccagggcgtg
1741 atggtgggca tgggtcagaa ggattcctat gtgggcgacg aggcccagag caagagaggc
1801 atcctcaccc tgaagtaccc catcgagcac ggcatcgtca ccaactggga cgacatggag
1861 aaaatctggc accacacctt ctacaatgag ctgcgtgtgg ctcccgagga gcaccccgtg
1921 ctgctgaccg aggcccccct gaaccccaag gccaaccgcg agaagatgac ccagatcatg
1981 tttgagacct tcaacacccc agccatgtac gttgctatcc aggctgtgct atccctgtac
2041 gcctctggcc gtaccactgg catcgtgatg gactccggtg acggggtcac ccacactgtg
2101 cccatctacg aggggtatgc cctcccccat gccatcctgc gtctggacct ggctggccgg
2161 gacctgactg actacctcat gaagatcctc accgagcgcg gctacagctt caccaccacg
2221 gccgagcggg aaatcgtgcg tgacattaag gagaagctgt gctacgtcgc cctggacttc
2281 gagcaagaga tggccacggc tgcttccagc tcctccctgg agaagagcta cgagctgcct
2341 gacggccagg tcatcaccat tggcaatgag cggttccgct gccctgaggc actcttccag
2401 ccttccttcc tgggcatgga gtcctgtggc atccacgaaa ctaccttcaa ctccatcatg
2461 aagtgtgacg tggacatccg caaagacctg tacgccaaca cagtgctgtc tggcggcacc
2521 accatgtacc ctggcattgc cgacaggatg cagaaggaga tcactgccct ggcacccagc
2581 acaatgaaga tcaagatcat tgctcctcct gagcgcaagt actccgtgtg gatcggcggc
2641 tccatcctgg cctcgctgtc caccttccag cagatgtgga tcagcaagca ggagtatgac
2701 gagtccggcc cctccatcgt ccaccgcaaa tgcttctagg gatccaccgg atctagataa
2761 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
2821 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
2881 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
2941 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgcgtaaat
3001 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
3061 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
3121 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
3181 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
3241 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
3301 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
3361 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
3421 cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg
3481 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
3541 ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac
3601 cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
3661 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
3721 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
3781 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
3841 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
3901 aagtagtgag gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg
3961 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
4021 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
4081 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
4141 tgccctgaat gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt
4201 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
4261 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
4321 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
4381 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
4441 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
4501 ggcgagcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
4561 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
4621 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
4681 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
4741 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
4801 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
4861 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
4921 atgctggagt tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata
4981 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg
5041 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg
5101 agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt
5161 tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca
5221 ggttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
5281 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
5341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
5401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
5461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
5521 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
5581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
5641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
5701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
5761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
5821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
5881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
5941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
6001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
6061 aaccgtatta ccgccatgca t
//
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