| 质粒名称: | pscALPSpuro-MmACE2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 158087 |
| 存储实验室: | Jeremy Luban |
|
载体骨架基本信息 |
|
| 载体名称: | pscALPS |
| 载体出品公司: | Clontech |
| 空载体大小: | 4038 bp |
| 完整质粒大小: | 10146bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Mouse ACE2 |
| 物种来源: | M. musculus (mouse) |
| 标签/融合蛋白: | Myc (C terminal on insert) |
|
文献引用方法 |
|
| 材料和方法部分: | pscALPSpuro-MmACE2 (Mouse) was a gift from Jeremy Luban (Addgene plasmid # 158087 ; http://n2t.net/addgene:158087 ; RRID:Addgene_158087) |
| 参考文献部分: | Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant. Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, Kyratsous CA, Dudkina NV, Shen K, Luban J. Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi: 10.1016/j.cell.2020.09.032. 10.1016/j.cell.2020.09.032 PubMed 32991842 |
买家导航
pscALPSpuro-MmACE2载体质粒基本信息
pscALPSpuro-MmACE2质粒图谱载体图谱和pscALPSpuro-MmACE2载体序列质粒序列多克隆位点信息
pscALPSpuro-MmACE2质粒载体简介
pscALPSpuro-MmACE2质粒序列载体序列
LOCUS pscALPSpuro-MmACE2 10146 bp DNA circular SYN 14-APR-2025
DEFINITION Expresses mouse ACE2 in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10146)
AUTHORS Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang
Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB,
Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC,
Kyratsous CA, Dudkina NV, Shen K, Luban J
TITLE Structural and Functional Analysis of the D614G SARS-CoV-2 Spike
Protein Variant.
JOURNAL Cell. 2020 Sep 15. pii: S0092-8674(20)31229-0. doi:
10.1016/j.cell.2020.09.032.
PUBMED 32991842
REFERENCE 2 (bases 1 to 10146)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2020
Sep 15. pii: S0092-8674(20)31229-0. doi:
10.1016/j.cell.2020.09.032."
COMMENT Sequence Label: pscALPSpuro-MmACE2 (Mouse)
FEATURES Location/Qualifiers
source 1..10146
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(64..84)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 69..398
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 249..384
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 311..330
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
enhancer 410..789
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 791..989
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 944..964
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 1007..1187
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1234..1359
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1856..2089
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2273..2317
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2615..2732
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2869..3276
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
CDS 5719..5748
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
CDS 6252..6851
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(6252..6271)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 6748..6768
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 6861..7449
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(6914..6934)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7332..7343)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 7967..8147
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(8218..8806)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8298..8317)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(8977..9768,9769..9837))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9600..9619
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9838..9942)
/gene="bla"
/label=AmpR promoter
primer_bind complement(10017..10036)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgc
61 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
121 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
181 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
241 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
301 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
361 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcgcgttg acattgatta
421 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
481 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
541 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
601 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
661 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
721 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
781 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
841 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
901 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
961 cgtgtacggt gggaggtcta tataagcagc gcgttttgcc tgtactgggt ctctctggtt
1021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
1081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
1141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
1201 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
1261 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
1321 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
1381 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
1441 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
1501 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
1561 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
1621 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
1681 accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg
1741 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1801 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1861 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
1921 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1981 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
2041 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
2101 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
2161 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaattaacaa
2221 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
2281 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
2341 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
2401 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
2461 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
2521 tggagagaga gacagagaca gatccattcg attagtgaac ggatcggcac tgcgtgcgcc
2581 aattctgcag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
2641 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
2701 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
2761 cagtttggtt aattaactgc agccccgata aaataaaaga ttttatttag tctccagaaa
2821 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
2881 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
2941 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
3001 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
3061 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
3121 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
3181 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
3241 ggcgcgccag tcctccgaca gactgagtcg cccgggggtc tagaagccgc caccatgtct
3301 tcaagctcct ggcttctcct gagcttggta gcggttacaa cggcgcagag tttgaccgag
3361 gaaaacgcga aaacatttct caataatttc aatcaagagg cagaggacct ttcttatcag
3421 agcagcctgg cgtcctggaa ctataacacg aatattacag aggaaaacgc tcaaaagatg
3481 agcgaggctg ccgccaaatg gagtgcattc tacgaggaac aatccaaaac cgcccagagc
3541 ttctcattgc aagagatcca aactccaatt atcaagaggc aattgcaagc acttcaacag
3601 tcagggagta gtgcattgtc tgcggacaag aacaagcagc tgaatacaat acttaatacg
3661 atgagtacca tctacagcac cggcaaggtc tgtaacccta aaaacccgca agaatgcttg
3721 cttcttgaac ctggacttga tgagataatg gccacgagta ctgactataa cagtcgcctc
3781 tgggcttggg aaggatggcg agcggaagta gggaagcaac tgagaccact gtatgaagaa
3841 tacgtagtct tgaagaacga gatggcacgg gcaaataact ataacgacta cggcgactac
3901 tggagaggag actacgaggc cgaaggagca gatgggtaca actacaatcg aaatcagctg
3961 attgaggacg tggagcgaac cttcgcggaa atcaagcctc tctatgagca cctgcatgcc
4021 tatgtcaggc gaaaacttat ggatacatat cctagttata tcagtccaac gggatgtctg
4081 ccggcccatc ttctcgggga catgtggggt agattttgga caaacctgta tcccctcacc
4141 gtcccctttg ctcagaagcc aaacattgat gtcaccgacg caatgatgaa tcaggggtgg
4201 gatgcagagc gcatctttca ggaggcggaa aaattttttg tatcagtagg gttgccacac
4261 atgacgcagg ggttctgggc aaacagcatg ttgacagaac ctgctgatgg gcgaaaggtt
4321 gtgtgccacc cgaccgcgtg ggaccttgga cacggtgatt tccgaatcaa gatgtgcaca
4381 aaggtaacaa tggacaactt tctcacagcc caccatgaga tgggccatat ccaatatgat
4441 atggcatatg caagacaacc attcctcctc cgcaatggtg cgaatgaggg ttttcatgaa
4501 gcggtgggcg aaatcatgtc tcttagcgcc gcgacaccta agcatttgaa gtctattggg
4561 cttttgccga gcgacttcca agaagactca gaaacagaga taaatttcct cttgaaacag
4621 gccttgacaa tagttggcac tctcccgttt acgtacatgc tggaaaagtg gcgatggatg
4681 gtttttcggg gagagattcc taaggaacag tggatgaaga agtggtggga gatgaagaga
4741 gaaattgttg gggtagttga gccgcttcca cacgacgaga cttattgcga cccggcttcc
4801 cttttccatg tttccaatga ctactctttt atacgatatt acaccagaac catataccaa
4861 ttccagtttc aagaggccct ttgccaggct gcgaagtaca acggatctct tcacaagtgt
4921 gacatatcta actcaacaga agcaggacaa aaactcttga aaatgctctc acttggaaac
4981 agcgaaccgt ggaccaaagc gctggagaat gtcgtgggag cacggaacat ggacgtgaaa
5041 cctctgctta attatttcca acctctgttt gattggctta aggaacagaa caggaacagc
5101 ttcgtcgggt ggaacacgga gtggtcccca tacgccgatc agtcaataaa ggtacggata
5161 agtttgaagt ccgcgctcgg cgccaatgct tacgaatgga ccaacaacga gatgtttctt
5221 ttccgcagct ctgtcgcata cgctatgcga aagtactttt ccatcataaa gaaccaaacc
5281 gttccgttcc ttgaagaaga tgttcgagtt tctgatctca aacctagagt ctcattttac
5341 ttctttgtta cgtccccaca aaacgtgtct gatgtgatcc caagatctga agtggaagat
5401 gccattcgga tgtccagggg cagaataaat gacgttttcg gtcttaatga caactcactg
5461 gagttcttgg gaatccaccc aactttggaa ccaccttatc agcccccagt gactatttgg
5521 ctcataatct tcggtgtggt tatggcactg gtcgtcgttg ggattattat actgattgta
5581 actggaatta aaggcagaaa aaagaaaaac gaaactaaac gggaagagaa cccatacgac
5641 agtatggaca ttgggaaggg tgagtcaaac gcgggcttcc aaaacagtga cgacgcgcaa
5701 acatctttcg gagcagctga gcagaagctg atcagcgagg aggacctgta ggaattctcg
5761 accttgactg gcggcgcgac cttgaggcct gcgttcgcct cagttgcccc ctctgtgcaa
5821 tggggagacg cgcctcatcg cttgacaacg gccgaagagc cgccgcgctt ccgtctcccg
5881 cgtgcgcgcg ccatgctgcc cacccccgtt ccgcactgac cctcccccgt gccccgcgtc
5941 ccgtactgcc gccccgcccc gagtcccatg ccgcagccac cgcgacggag cccgcaggcg
6001 ggaacctgcc tccgcgcgtt agcgcgcacg cgcgcctcat gtgtcgtccc catcagcgcc
6061 ggcttccgtc tataggccag atgcactgtc actctggcga agtcgcagac ccgattggcc
6121 gggacggagg cgcgagaccg ggttgcgggc ggggccgaac gtggtataaa aggggcggga
6181 ggccaggctc gtgccgtttt gcagacgcca ccgccgagga aaaccgtgta ctattagcca
6241 cgcgtgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
6301 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
6361 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
6421 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
6481 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
6541 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
6601 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
6661 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
6721 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
6781 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
6841 ccggtgcctg agcgatcgct aatcaacctc tggattacaa aatttgtgaa agattgactg
6901 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
6961 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
7021 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
7081 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
7141 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
7201 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaagctga
7261 cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
7321 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
7381 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
7441 cctccccgct taatcgcgtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
7501 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
7561 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
7621 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
7681 tatccttgat ctgtggatct accacacaca aggctacttc cctgattggc agaactacac
7741 accagggcca gggatcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
7801 tgagcaagag aaggtagaag aagccaatga aggagagaac acccgcttgt tacaccctgt
7861 gagcctgcat gggatggatg acccggagag agaagtatta gagtggaggt ttgacagccg
7921 cctagcattt catcacatgg cccgagagct gcatccggac tgtactgggt ctctctggtt
7981 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
8041 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
8101 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcaggg cccgtttcat
8161 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
8221 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
8281 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
8341 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
8401 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
8461 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
8521 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
8581 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
8641 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
8701 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
8761 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
8821 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
8881 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
8941 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
9001 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
9061 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
9121 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
9181 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
9241 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
9301 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
9361 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
9421 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
9481 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
9541 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
9601 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
9661 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
9721 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
9781 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
9841 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
9901 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
9961 gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca
10021 atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc
10081 gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc
10141 atgaag
//
