| 质粒名称: | pAAVLINK-EFS-HA-5'-ZNF292 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001701 |
| 产品用途: | 5' AAVLINK plasmid for ZNF292 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EFS |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | ZNF292 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EFS-HA-5'-ZNF292
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EFS-HA-5'-ZNF292载体质粒基本信息
pAAVLINK-EFS-HA-5'-ZNF292质粒图谱载体图谱和pAAVLINK-EFS-HA-5'-ZNF292载体序列质粒序列多克隆位点信息
pAAVLINK-EFS-HA-5'-ZNF292质粒载体简介
pAAVLINK-EFS-HA-5'-ZNF292质粒序列载体序列
LOCUS pAAVLINK-EFS-HA-5'-ZNF292 7193 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7193)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7193
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..356
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 371..397
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 404..4333
/codon_start=1
/label=ZNF292
/translation="ADEEAEQERLSCGEGGCVAELQRLGERLQELELQLRESRVPAVEA
ATDYCQQLCQTLLEYAEKWKTSEDPLPLLEVYTVAIQSYVKARPYLTSECENVALVLER
LALSCVELLLCLPVELSDKQWEQFQTLVQVAHEKLMENGSCELHFLATLAQETGVWKNP
VLCTILSQEPLDKDKVNEFLAFEGPILLDMRIKHLIKTNQLSQATALAKLCSDHPEIGI
KGSFKQTYLVCLCTSSPNGKLIEEISEVDCKDALEMICNLESEGDEKSALVLCTAFLSR
QLQQGDMYCAWELTLFWSKLQQRVEPSIQVYLERCRQLSLLTKTVYHIFFLIKVINSET
EGAGLATCIELCVKALRLESTENTEVKISICKTISCLLPDDLEVKRACQLSEFLIEPTV
DAYYAVEMLYNQPDQKYDEENLPIPNSLRCELLLVLKTQWPFDPEFWDWKTLKRQCLAL
MGEEASIVSSIDELNDSEVYEKVVDYQEESKETSMNGLSGGVGANSGLLKDIGDEKQKK
REIKQLRERGFISARFRNWQAYMQYCVLCDKEFLGHRIVRHAQKHYKDGIYSCPICAKN
FNSKETFVPHVTLHVKQSSKERLAAMKPLRRLGRPPKITTTNENQKTNTVAKQEQRPIK
KNSLYSTDFIVFNDNDGSDDENDDKDKSYEPEVIPVQKPVPVNEFNCPVTFCKKGFKYF
KNLIAHVKGHKDNEDAKRFLEMQSKKVICQYCRRHFVSVTHLNDHLQMHCGSKPYICIQ
MKCKAGFNSYAELLTHRKEHQVFRAKCMFPKCGRIFSEAYLLYDHEAQHYNTYTCKFTG
CGKVYRSQGELEKHLDDHSTPPEKVLPPEAQLNSSGDSIQPSEVNQNTAENIEKERSML
PSENNIENSLLADRSDAWDKSKAESAVTKQDQISASELRQANGPLSNGLENPATTPLLQ
SSEVAVSIKVSLNQGIEDNFGKQENSTVEGSGEALVTDLHTPVEDTCNDLCHPGFQERK
EQDCFNDAHVTQNSLVNSETLKIGDLTPQNLERQVNNLMTFSVQNQAAFQNNLPTSKFE
CGDNVKTSSNLYNLPLKTLESIAFVPPQSDLSNSLGTPSVPPKAPVQKFSCQVEGCTRT
YNSSQSIGKHMKTAHPDQYAAFKMQRKSKKGQKANNLNTPNNGKFVYFLPSPVNSSNPF
FTSQTKANGNPACSAQLQHVSPPIFPAHLASVSTPLLSSMESVINPNITSQDKNEQGGM
LCSQMENLPSTALPAQMEDLTKTVLPLNIDSGSDPFLPLPAESSSMSLFPSPADSGTNS
VFSQLENNTNHYSSQIEGNTNSSFLK"
misc_feature 2900..2905
/label=SDS
misc_feature 2918..2923
/label=SDS
misc_feature 4320..4325
/label=SDS
intron 4334..4374
/label=Splicing Donor
misc_feature 4378..4411
/label=lox2272-JT1571
repeat_region 4456..4596
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4456..4585
/label=AAV2 ITR
rep_origin 4671..5126
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5408..5512
/gene="bla"
/label=AmpR promoter
CDS 5513..6373
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6544..7132
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgggcag agcgcacatc gcccacagtc cccgagaagt
181 tggggggagg ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg
241 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
301 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagcgta
361 cgccaccatg tacccttatg acgtacctga ctatgctgga tctgcggacg aagaggccga
421 gcaggagagg ttgagttgcg gcgaaggcgg ctgcgtcgcg gagctgcagc gcctgggcga
481 gcggctccag gagctggagc tacagctgcg ggagagccgg gtaccggccg tggaagcggc
541 caccgactac tgtcagcagc tgtgccagac actcctagaa tatgcagaga aatggaaaac
601 ttcagaagat cctttacctt tattggaggt atacacagtg gctatccaaa gttatgttaa
661 agcccgacct tatcttacct ctgaatgtga aaatgtagcc ttggttctgg aacgcttggc
721 attaagctgt gttgaacttt tactgtgtct gcctgttgag ttatcagata aacagtggga
781 acaatttcag acactggtgc aggtagctca tgaaaagctg atggagaatg gcagctgtga
841 attgcatttt ttagctactc tagctcaaga gactggggtg tggaaaaacc cggtactgtg
901 cactattctt tcccaggaac cattggataa ggataaagtg aatgaatttt tagcttttga
961 gggtcccatc ttgttggata tgagaattaa acatctaatc aaaacaaatc agttaagtca
1021 agcaactgct ctagcaaagc tgtgttctga ccatccagag attggcataa aaggtagttt
1081 taagcaaact taccttgtct gtctttgtac atcatcacca aatggaaagt taatcgaaga
1141 gatttcagaa gttgattgca aagatgcact ggaaatgatc tgtaacttag aatctgaggg
1201 tgatgaaaaa agcgctcttg ttttatgtac tgcgtttttg tcacgtcagc tccaacaagg
1261 agatatgtac tgcgcttggg aacttactct cttttggagt aaattacaac aaagagtaga
1321 accatctata caagtgtacc ttgagaggtg tcgtcaactt tctttgttaa cgaaaacagt
1381 atatcacatt ttcttcctga ttaaagttat taattcagag actgaagggg ctggacttgc
1441 tacctgtata gaactgtgtg taaaggctct tcgcttggag tctacagaaa atactgaagt
1501 gaaaatatct atttgcaaga ccatttcatg tttgttgcct gatgatctgg aagttaaacg
1561 tgcttgtcaa ctgagtgaat ttcttattga gcctacagta gatgcgtatt atgctgtgga
1621 aatgttgtat aatcagccag accagaaata tgatgaagag aatcttccaa taccaaattc
1681 tttacgctgt gagctgttac ttgtattgaa aactcaatgg ccctttgatc cagaattctg
1741 ggattggaaa accttgaaac gacaatgtct tgcattaatg ggagaagaag catccattgt
1801 gtcttcaata gatgaactaa atgacagtga agtatatgaa aaagtggtag actaccaaga
1861 agagagtaaa gaaacttcta tgaatgggct ttctggtgga gttggtgcta attctggcct
1921 tcttaaagac attggtgatg aaaagcagaa gaagagagag ataaaacagt taagagagag
1981 gggatttata tctgctcggt ttaggaattg gcaagcctac atgcagtatt gtgtgttgtg
2041 tgacaaagaa ttccttggtc acagaatagt acgacatgct cagaaacatt acaaagatgg
2101 aatttatagt tgccccatat gtgcaaagaa ctttaattct aaagaaactt ttgtccctca
2161 tgtcacactg catgttaaac aatctagtaa agagagacta gcagctatga aaccattaag
2221 aagattggga aggcctccaa agatcacaac taccaatgaa aatcagaaga ctaatactgt
2281 ggctaaacag gagcagcgac ctataaaaaa gaatagtctc tattcaacag attttatagt
2341 gtttaatgac aatgatggtt cagatgatga gaatgatgac aaagataaat cctatgagcc
2401 agaagtgatt ccagtccaga aaccagtacc tgttaatgaa tttaattgcc ctgtaacttt
2461 ttgtaaaaag ggctttaagt actttaaaaa tttaattgct catgtgaagg ggcataaaga
2521 taatgaagac gccaagcgct ttcttgaaat gcagagcaaa aaagttattt gccagtactg
2581 taggcggcat tttgtgagtg ttactcatct caatgatcac ttacagatgc actgtggcag
2641 taaaccatat atctgtatac agatgaaatg taaagctggt tttaatagtt acgccgagct
2701 tttaacccac cgaaaggagc atcaagtctt tagagcaaaa tgtatgtttc ctaaatgtgg
2761 aagaattttt tcggaagctt atttactata tgatcatgaa gcacaacatt ataatacgta
2821 cacttgtaag ttcacaggtt gtggtaaagt ttatcgttct cagggtgagc tggaaaagca
2881 tctggatgat cacagtactc ctcctgaaaa agtgctgcct cctgaagccc aacttaattc
2941 atctggagat tccattcagc cttctgaagt gaatcagaac acagcagaga atattgagaa
3001 agaaagatct atgcttcctt cagaaaataa cattgaaaac agcttactag cagatagaag
3061 tgatgcttgg gataaaagca aagcagaatc agctgtgacc aaacaagacc agatttctgc
3121 ctctgagctc aggcaagcta atggaccatt gtcaaatggt ttggaaaacc ctgctactac
3181 tcctctactt caatccagtg aagtagctgt gtccattaag gtgtctctca atcaggggat
3241 tgaggataac tttggaaagc aagaaaactc aactgtggaa ggcagtggtg aagcactggt
3301 cacagactta catacgccag ttgaagatac ttgtaatgat ttgtgtcatc caggtttcca
3361 ggagagaaaa gaacaagatt gctttaatga tgcccatgtt actcagaatt ctttagtaaa
3421 ttcagaaact ctcaaaatag gtgaccttac cccacaaaac ttagaaagac aagtgaacaa
3481 cttgatgacc ttttctgtgc aaaatcaggc agcatttcaa aacaatttac caacttccaa
3541 atttgaatgt ggagataatg ttaaaacatc atccaatctt tataatttac ctcttaagac
3601 attagaaagt attgcatttg ttccaccgca gtccgaccta agtaattcat taggaactcc
3661 atcagtgcct ccaaaagctc cagttcagaa attcagctgc caggtcgagg gatgtactcg
3721 aacctataat tcttcacaga gtattgggaa acacatgaag acagcacacc ctgaccaata
3781 tgctgcattt aaaatgcagc gcaaaagtaa aaaaggtcag aaagctaaca acttaaatac
3841 accaaataat ggaaagtttg tttatttttt gccatcaccg gtgaacagct caaatccatt
3901 ttttacatca cagaccaaag ccaatgggaa tcctgcttgt tcggcccagt tgcagcatgt
3961 ctcgccaccc atttttccag ctcatttagc aagtgtgtca actccattgt tgtcctcaat
4021 ggaaagtgtc ataaatccaa atataacttc tcaggataaa aatgaacaag gtggtatgtt
4081 atgttcccaa atggaaaatt tacctagtac tgccttgcca gcacaaatgg aagatctaac
4141 caaaacagtt ctgcctttga atattgacag tggctcagat cctttccttc ctttacctgc
4201 agaaagtagt tcaatgtctc tcttcccttc accagcagat agtgggacta attctgtttt
4261 ttcccaactg gaaaataata caaatcatta ttcctcacag attgaaggaa acactaattc
4321 ctcctttcta aaggtaagta tcaaggttac aagacaggtt taaggagacc aataagctat
4381 tattcgtata ggatacttta tacgaagtta ttaagttatg gagaccggtg tggcacgtgc
4441 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
4501 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
4561 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
4621 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
4681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
4741 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
4801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
4861 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
4921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
4981 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
5041 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
5101 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
5161 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
5221 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
5281 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
5341 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
5401 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
5461 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
5521 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
5581 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
5641 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
5701 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
5761 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
5821 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
5881 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
5941 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
6001 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
6061 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
6121 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
6181 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
6241 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
6301 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
6361 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
6421 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
6481 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
6541 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
6601 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
6661 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
6721 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
6781 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
6841 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
6901 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
6961 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
7021 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
7081 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
7141 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
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