pcambia1300-EGFP

LOCUS       pCambia1300-EGFP               10503 bp DNA     circular SYN 12-JUN-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Ming
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2025 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10503
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        190..535
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
                     /note="color: #ffffff; direction: RIGHT"
     CDS             573..1292
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1:  573 ..  575 / #05fd14
                        2:  576 ..  578 / #05fd14 / 1a
                        3:  579 .. 1292 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1605..1621)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    1824..1848
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     CDS             3148..3777
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /note="color: #993366"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             4211..5278
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /note="color: #993366"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      5344..5538
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /note="color: #ffff00"
     misc_feature    5882..6022
                     /label=bom
                     /note="basis of mobility region from pBR322"
                     /note="color: #a6acb3"
     rep_origin      complement(6208..6796)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6883..7677)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /note="color: #ccffcc"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    8102..8126
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
                     /note="color: #ffe4c4"
     polyA_signal    8204..8378
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
                     /note="color: #a6acb3"
     CDS             complement(8418..9443)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(9511..10188)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
                     /note="color: #ffffff; direction: LEFT"
     protein_bind    10379..10400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        10415..10445
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 10415 .. 10420 / #ffffff / -35
                        2: 10421 .. 10438 / #ffffff
                        3: 10439 .. 10445 / #ffffff / -10"
     protein_bind    10453..10469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     10477..10493
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 aattcccatg gagtcaaaga ttcaaataga ggacctaaca gaactcgccg taaagactgg
       61 cgaacagttc atacagagtc tcttacgact caatgacaag aagaaaatct tcgtcaacat
      121 ggtggagcac gacacgcttg tctactccaa aaatatcaaa gatacagtct cagaagacca
      181 aagggcaatt gagacttttc aacaaagggt aatatccgga aacctcctcg gattccattg
      241 cccagctatc tgtcacttta ttgtgaagat agtggaaaag gaaggtggct cctacaaatg
      301 ccatcattgc gataaaggaa aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa
      361 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      421 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
      481 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacagggta
      541 cccggggatc ctctagagtc gacactagta ccatggtgag caagggcgag gagctgttca
      601 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      661 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      721 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      781 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      841 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      901 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      961 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     1021 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     1081 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     1141 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     1201 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
     1261 tcactctcgg catggacgag ctgtacaagt aactgcaggt tcgagtatta tggcattggg
     1321 aaaactgttt ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt
     1381 ttcgctatcg aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct
     1441 tttgttcatt ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt
     1501 gaaattataa gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct
     1561 ctaatgaccg aagttaatat gaggagtaaa acactaagct tggcactggc cgtcgtttta
     1621 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     1681 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     1741 cgcagcctga atggcgaatg ctagagcagc ttgagcttgg atcagattgt cgtttcccgc
     1801 cttcagttta aactatcagt gtttgacagg atatattggc gggtaaacct aagagaaaag
     1861 agcgtttatt agaataacgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
     1921 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc
     1981 tccgctgcta tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca
     2041 tgtcgcacaa gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc
     2101 ttgtcgcgtg ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg
     2161 ccatgaacaa gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc
     2221 aggacttgac caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg
     2281 agaagatcac cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac
     2341 gccctggcga cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc
     2401 tactggacat tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc
     2461 cgtgggccga caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg
     2521 ccgagttcga gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg
     2581 cccgaggcgt gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc
     2641 gcgagctgat cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc
     2701 atcgctcgac cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca
     2761 ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg
     2821 agaatgaacg ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt
     2881 tttcattacc gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc
     2941 cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct
     3001 ggcggcctgg ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg
     3061 atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt
     3121 aaataaacaa atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc
     3181 gggtcaggca agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc
     3241 gatgttctgt tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg
     3301 gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag
     3361 gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct
     3421 gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac
     3481 atatgggcca ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga
     3541 aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag
     3601 gttgccgagg cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc
     3661 gtgagctacc caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc
     3721 gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt
     3781 aatgaggtaa agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg
     3841 cacgcagcag caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg
     3901 tcaactttca gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag
     3961 gcaagaccat taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca
     4021 aatgaataaa tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa
     4081 caaccaggca ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc
     4141 gtaagcggct gggttgtctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa
     4201 tcggcgtgac ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
     4261 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
     4321 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
     4381 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
     4441 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
     4501 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
     4561 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
     4621 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
     4681 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
     4741 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
     4801 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
     4861 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
     4921 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
     4981 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
     5041 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
     5101 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
     5161 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
     5221 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
     5281 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggtctctt
     5341 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
     5401 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
     5461 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
     5521 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
     5581 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
     5641 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
     5701 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
     5761 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     5821 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     5881 gcgcagccat gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc
     5941 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6001 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6061 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     6121 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     6181 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     6241 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     6301 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     6361 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     6421 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     6481 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     6541 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     6601 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     6661 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     6721 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     6781 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     6841 cgaaaactca cgttaaggga ttttggtcat gcattctagg tactaaaaca attcatccag
     6901 taaaatataa tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag
     6961 ctcgacatac tgttcttccc cgatatcctc cctgatcgac cggacgcaga aggcaatgtc
     7021 ataccacttg tccgccctgc cgcttctccc aagatcaata aagccactta ctttgccatc
     7081 tttcacaaag atgttgctgt ctcccaggtc gccgtgggaa aagacaagtt cctcttcggg
     7141 cttttccgtc tttaaaaaat catacagctc gcgcggatct ttaaatggag tgtcttcttc
     7201 ccagttttcg caatccacat cggccagatc gttattcagt aagtaatcca attcggctaa
     7261 gcggctgtct aagctattcg tatagggaca atccgatatg tcgatggagt gaaagagcct
     7321 gatgcactcc gcatacagct cgataatctt ttcagggctt tgttcatctt catactcttc
     7381 cgagcaaagg acgccatcgg cctcactcat gagcagattg ctccagccat catgccgttc
     7441 aaagtgcagg acctttggaa caggcagctt tccttccagc catagcatca tgtccttttc
     7501 ccgttccaca tcataggtgg tccctttata ccggctgtcc gtcattttta aatataggtt
     7561 ttcattttct cccaccagct tatatacctt agcaggagac attccttccg tatcttttac
     7621 gcagcggtat ttttcgatca gttttttcaa ttccggtgat attctcattt tagccattta
     7681 ttatttcctt cctcttttct acagtattta aagatacccc aagaagctaa ttataacaag
     7741 acgaactcca attcactgtt ccttgcattc taaaacctta aataccagaa aacagctttt
     7801 tcaaagttgt tttcaaagtt ggcgtataac atagtatcga cggagccgat tttgaaaccg
     7861 cggtgatcac aggcagcaac gctctgtcat cgttacaatc aacatgctac cctccgcgag
     7921 atcatccgtg tttcaaaccc ggcagcttag ttgccgttct tccgaatagc atcggtaaca
     7981 tgagcaaagt ctgccgcctt acaacggctc tcccgctgac gccgtcccgg actgatgggc
     8041 tgcctgtatc gagtggtgat tttgtgccga gctgccggtc ggggagctgt tggctggctg
     8101 gtggcaggat atattgtggt gtaaacaaat tgacgcttag acaacttaat aacacattgc
     8161 ggacgttttt aatgtactga attaacgccg aattaattcg ggggatctgg attttagtac
     8221 tggattttgg ttttaggaat tagaaatttt attgatagaa gtattttaca aatacaaata
     8281 catactaagg gtttcttata tgctcaacac atgagcgaaa ccctatagga accctaattc
     8341 ccttatctgg gaactactca cacattatta tggagaaact cgagcttgtc gatcgacaga
     8401 tccggtcggc atctactcta tttctttgcc ctcggacgag tgctggggcg tcggtttcca
     8461 ctatcggcga gtacttctac acagccatcg gtccagacgg ccgcgcttct gcgggcgatt
     8521 tgtgtacgcc cgacagtccc ggctccggat cggacgattg cgtcgcatcg accctgcgcc
     8581 caagctgcat catcgaaatt gccgtcaacc aagctctgat agagttggtc aagaccaatg
     8641 cggagcatat acgcccggag tcgtggcgat cctgcaagct ccggatgcct ccgctcgaag
     8701 tagcgcgtct gctgctccat acaagccaac cacggcctcc agaagaagat gttggcgacc
     8761 tcgtattggg aatccccgaa catcgcctcg ctccagtcaa tgaccgctgt tatgcggcca
     8821 ttgtccgtca ggacattgtt ggagccgaaa tccgcgtgca cgaggtgccg gacttcgggg
     8881 cagtcctcgg cccaaagcat cagctcatcg agagcctgcg cgacggacgc actgacggtg
     8941 tcgtccatca cagtttgcca gtgatacaca tggggatcag caatcgcgca tatgaaatca
     9001 cgccatgtag tgtattgacc gattccttgc ggtccgaatg ggccgaaccc gctcgtctgg
     9061 ctaagatcgg ccgcagcgat cgcatccata gcctccgcga ccggttgtag aacagcgggc
     9121 agttcggttt caggcaggtc ttgcaacgtg acaccctgtg cacggcggga gatgcaatag
     9181 gtcaggctct cgctaaactc cccaatgtca agcacttccg gaatcgggag cgcggccgat
     9241 gcaaagtgcc gataaacata acgatctttg tagaaaccat cggcgcagct atttacccgc
     9301 aggacatatc cacgccctcc tacatcgaag ctgaaagcac gagattcttc gccctccgag
     9361 agctgcatca ggtcggagac gctgtcgaac ttttcgatca gaaacttctc gacagacgtc
     9421 gcggtgagtt caggcttttt catatctcat tgcccccccg gatctgcgaa agctcgagag
     9481 agatagattt gtagagagag actggtgatt tcagcgtgtc ctctccaaat gaaatgaact
     9541 tccttatata gaggaaggtc ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca
     9601 gtggagatat cacatcaatc cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc
     9661 acgatgctcc tcgtgggtgg gggtccatct ttgggaccac tgtcggcaga ggcatcttga
     9721 acgatagcct ttcctttatc gcaatgatgg catttgtagg tgccaccttc cttttctact
     9781 gtccttttga tgaagtgaca gatagctggg caatggaatc cgaggaggtt tcccgatatt
     9841 accctttgtt gaaaagtctc aatagccctt tggtcttctg agactgtatc tttgatattc
     9901 ttggagtaga cgagagtgtc gtgctccacc atgttatcac atcaatccac ttgctttgaa
     9961 gacgtggttg gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg
    10021 ggaccactgt cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat
    10081 ttgtaggtgc caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa
    10141 tggaatccga ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg
    10201 tcttctgaga ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg
    10261 ttggcaagct gctctagcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
    10321 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
    10381 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
    10441 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
    10501 acg
//