pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRD4-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BRD4-FLAG               6831 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 19, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3544
                     /codon_start=1
                     /gene="BRD4"
                     /gene_synonym="CAP; FSHRG4; HUNK1; HUNKI; MCAP"
                     /product="bromodomain-containing protein 4 isoform long"
                     /EC_number="3.1.1.31"
                     /label=BRD4
                     /note="isoform long is encoded by transcript variant 4; 
                     chromosome-associated protein; bromodomain-containing 
                     protein 4; mitotic chromosome-associated protein; female 
                     sterile homeotic related gene 4"
                     /db_xref="GI:1825629150"
                     /db_xref="CCDS:CCDS12328.1"
                     /db_xref="GeneID:23476"
                     /db_xref="HGNC:HGNC:13575"
                     /db_xref="MIM:608749"
                     /protein_id="NP_001366220.1"
                     /translation="GFSSSESESSSESSSSDSEDSETEMAPKSKKKGHPGREQKKHHHH
                     HHQQMQQAPAPVPQQPPPPPQQPPPPPPPQQQQQPPPPPPPPSMPQQAAPAMKSSPPPF
                     IATQVPVLEPQLPGSVFDPIGHFTQPILHLPQPELPPHLPQPPEHSTPPHLNQHAVVSP
                     PALHNALPQQPSRPSNRAAALPPKPARPPAVSPALTQTPLLPQPPMAQPPQVLLEDEEP
                     PAPPLTSMQMQLYLQQLQKVQPPTPLLPSVKVQSQPPPPLPPPPHPSVQQQLQQQPPPP
                     PPPQPQPPPQQQHQPPPRPVHLQPMQFSTHIQQPPPPQGQQPPHPPPGQQPPPPQPAKP
                     QQVIQHHHSPRHHKSDPYSTGHLREAPSPLMIHSPQMSQFQSLTHQSPPQQNVQPKKQE
                     LRAASVVQPQPLVVVKEEKIHSPIIRSEPFSPSLRPEPPKHPESIKAPVHLPQRPEMKP
                     VDVGRPVIRPPEQNAPPPGAPDKDKQKQEPKTPVAPKKDLKIKNMGSWASLVQKHPTTP
                     SSTAKSSSDSFEQFRRAAREKEEREKALKAQAEHAEKEKERLRQERMRSREDEDALEQA
                     RRAHEEARRRQEQQQQQRQEQQQQQQQQAAAVAAAATPQAQSSQPQSMLDQQRELARKR
                     EQERRRREAMAATIDMNFQSDLLSIFEENLF"
     CDS             1670..1687
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2790..2795
                     /label=SDS
     misc_feature    3096..3101
                     /label=SDS
     misc_feature    3288..3293
                     /label=SDS
     CDS             3551..3616
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3620..4051
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3764..3769
                     /label=SDS
     repeat_region   4094..4234
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4094..4223
                     /label=AAV2 ITR
     repeat_region   4095..4235
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4309..4764
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5046..5150
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5151..6011
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6182..6770
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggct tctcgtcctc
     1561 agagtcggag agctccagtg agtccagctc ctctgacagc gaagactccg aaacagagat
     1621 ggctccgaag tcaaaaaaga aggggcaccc cgggagggag cagaagaagc accatcatca
     1681 ccaccatcag cagatgcagc aggccccggc tcctgtgccc cagcagccgc ccccgcctcc
     1741 ccagcagccc ccaccgcctc cacctccgca gcagcaacag cagccgccac ccccgcctcc
     1801 cccaccctcc atgccgcagc aggcagcccc ggcgatgaag tcctcgcccc cacccttcat
     1861 tgccacccag gtgcccgtcc tggagcccca gctcccaggc agcgtctttg accccatcgg
     1921 ccacttcacc cagcccatcc tgcacctgcc gcagcctgag ctgccccctc acctgcccca
     1981 gccgcctgag cacagcactc caccccatct caaccagcac gcagtggtct ctcctccagc
     2041 tttgcacaac gcactacccc agcagccatc acggcccagc aaccgagccg ctgccctgcc
     2101 tcccaagccc gcccggcccc cagccgtgtc accagccttg acccaaacac ccctgctccc
     2161 acagcccccc atggcccaac ccccccaagt gctgctggag gatgaagagc cacctgcccc
     2221 acccctcacc tccatgcaga tgcagctgta cctgcagcag ctgcagaagg tgcagccccc
     2281 tacgccgcta ctcccttccg tgaaggtgca gtcccagccc ccaccccccc tgccgccccc
     2341 accccacccc tctgtgcagc agcagctgca gcagcagccg ccaccacccc caccacccca
     2401 gccccagcct ccaccccagc agcagcatca gccccctcca cggcccgtgc acttgcagcc
     2461 catgcagttt tccacccaca tccaacagcc cccgccaccc cagggccagc agccccccca
     2521 tccgccccca ggccagcagc cacccccgcc gcagcctgcc aagcctcagc aagtcatcca
     2581 gcaccaccat tcaccccggc accacaagtc ggacccctac tcaaccggtc acctccgcga
     2641 agccccctcc ccgcttatga tacattcccc ccagatgtca cagttccaga gcctgaccca
     2701 ccagtctcca ccccagcaaa acgtccagcc taagaaacag gagctgcgtg ctgcctccgt
     2761 ggtccagccc cagcccctcg tggtggtgaa ggaggagaag atccactcac ccatcatccg
     2821 cagcgagccc ttcagcccct cgctgcggcc ggagcccccc aagcacccgg agagcatcaa
     2881 ggcccccgtc cacctgcccc agcggccgga aatgaagcct gtggatgtcg ggaggcctgt
     2941 gatccggccc ccagagcaga acgcaccgcc accaggggcc cctgacaagg acaaacagaa
     3001 acaggagccg aagactccag ttgcgcccaa aaaggacctg aaaatcaaga acatgggctc
     3061 ctgggccagc ctagtgcaga agcatccgac caccccctcc tccacagcca agtcatccag
     3121 cgacagcttc gagcagttcc gccgcgccgc tcgggagaaa gaggagcgtg agaaggccct
     3181 gaaggctcag gccgagcacg ctgagaagga gaaggagcgg ctgcggcagg agcgcatgag
     3241 gagccgagag gacgaggatg cgctggagca ggcccggcgg gcccatgagg aggcacgtcg
     3301 gcgccaggag cagcagcagc agcagcgcca ggagcaacag cagcagcagc aacagcaagc
     3361 agctgcggtg gctgccgccg ccaccccaca ggcccagagc tcccagcccc agtccatgct
     3421 ggaccagcag agggagttgg cccggaagcg ggagcaggag cgaagacgcc gggaagccat
     3481 ggcagctacc attgacatga atttccagag tgatctattg tcaatatttg aagaaaatct
     3541 tttctgtaca gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa
     3601 ggatgacgat gacaagtaac tcgagataat caacctctgg attacaaaat ttgtgaaaga
     3661 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     3721 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     3781 tggttagttc ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3841 gctcggctgt tgggcactga caattccgtg gtgtttattt gtgaaatttg tgatgctatt
     3901 gctttatttg taaccatcta gctttatttg tgaaatttgt gatgctattg ctttatttgt
     3961 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     4021 ggttcagggg gagatgtggg aggtttttta aagatctgga gaccggtgtg gcacgtgcgg
     4081 accgagcggc cgcaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     4141 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     4201 tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc tgatgcggta ttttctcctt
     4261 acgcatctgt gcggtatttc acaccgcata cgtcaaagca accatagtac gcgccctgta
     4321 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     4381 gcgccttagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     4441 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     4501 acctcgaccc caaaaaactt gatttgggtg atggttcacg tagtgggcca tcgccctgat
     4561 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     4621 aaactggaac aacactcaac tctatctcgg gctattcttt tgatttataa gggattttgc
     4681 cgatttcggt ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     4741 acaaaatatt aacgtttaca attttatggt gcactctcag tacaatctgc tctgatgccg
     4801 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4861 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4921 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     4981 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     5041 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     5101 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     5161 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     5221 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     5281 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5341 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5401 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5461 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5521 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5581 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5641 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5701 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5761 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5821 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5881 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5941 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     6001 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     6061 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     6121 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     6181 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     6241 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6301 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6361 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6421 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6481 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6541 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6601 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6661 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6721 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6781 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg t
//