| 质粒名称: | pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA2-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001969 |
| 产品用途: | 3' AAVLINK plasmid for SMARCA2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | SCP1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | SMARCA2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA2-FLAG载体质粒基本信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA2-FLAG质粒图谱载体图谱和pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA2-FLAG质粒载体简介
pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA2-FLAG质粒序列载体序列
LOCUS pAAVLINKv2-SCP1-Cre-UDeg3a-3'-SMARCA2-FLAG 6455 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6455)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 19, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6455
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
misc_feature 145..225
/label=SCP1
CDS 237..1265
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
CDS 1281..1370
/codon_start=1
/label=UDeg3a
/translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
misc_feature 1295..1300
/label=SDS
misc_feature 1298..1303
/label=SDS
intron 1374..1414
/label=Splicing Donor
protein_bind 1421..1454
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1455..1546
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1547..3168
/codon_start=1
/gene="SMARCA2"
/gene_synonym="BAF190; BIS; BRM; hBRM; hSNF2a; NCBRS;SNF2;
SNF2L2; SNF2LA; Sth1p; SWI2"
/product="probable global transcription activator
SNF2L2isoform a"
/label=SMARCA2
/note="isoform a is encoded by transcript variant 1;global
transcription activator homologous sequence; SNF2-alpha;
sucrose nonfermenting 2-like protein 2; SNF2/SWI2-like
protein 2; SWI/SNF-related matrix-associated
actin-dependent regulator of chromatin a2; brahma homolog;
probable global transcription activator SNF2L2; BAF190B;
protein brahma homolog; BRG1-associated factor 190B;
ATP-dependent helicase SMARCA2; putative global
transcription activator SNF2L2"
/db_xref="GI:48255900"
/db_xref="CCDS:CCDS34977.1"
/db_xref="GeneID:6595"
/db_xref="HGNC:HGNC:11098"
/db_xref="MIM:600014"
/protein_id="NP_003061.3"
/translation="KFELLDRILPKLRATNHRVLLFCQMTSLMTIMEDYFAFRNFLYLR
LDGTTKSEDRAALLKKFNEPGSQYFIFLLSTRAGGLGLNLQAADTVVIFDSDWNPHQDL
QAQDRAHRIGQQNEVRVLRLCTVNSVEEKILAAAKYKLNVDQKVIQAGMFDQKSSSHER
RAFLQAILEHEEENEEEDEVPDDETLNQMIARREEEFDLFMRMDMDRRREDARNPKRKP
RLMEEDELPSWIIKDDAEVERLTCEEEEEKIFGRGSRQRRDVDYSDALTEKQWLRAIED
GNLEEMEEEVRLKKRKRRRNVDKDPAKEDVEKAKKRRGRPPAEKLSPNPPKLTKQMNAI
IDTVINYKDRCNVEKVPSNSQLEIEGNSSGRQLSEVFIQLPSRKELPEYYELIRKPVDF
KKIKERIRNHKYRSLGDLEKDVMLLCHNAQTFNLEGSQIYEDSIVLQSVFKSARQKIAK
EEESEDESNEEEEEEDEEESESEAKSVKVKIKLNKKDDKGRDKGKGKKRPNRGKAKPVV
SDFDSDEEQDEREQSEGSGTDDE"
misc_feature 2069..2074
/label=SDS
misc_feature 2225..2230
/label=SDS
misc_feature 2240..2245
/label=SDS
misc_feature 2294..2299
/label=SDS
misc_feature 2954..2959
/label=SDS
CDS 3175..3240
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3244..3675
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3388..3393
/label=SDS
repeat_region 3718..3858
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3718..3847
/label=AAV2 ITR
repeat_region 3719..3859
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 3933..4388
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4670..4774
/gene="bla"
/label=AmpR promoter
CDS 4775..5635
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5806..6394
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
481 gtctggcagt aaaaactatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggaa gtttgagctg
1561 cttgatcgta ttctgccaaa attgagagcg actaatcacc gagtgctgct tttctgccag
1621 atgacatctc tcatgaccat catggaggat tattttgctt ttcggaactt cctttaccta
1681 cgccttgatg gcaccaccaa gtctgaagat cgtgctgctt tgctgaagaa attcaatgaa
1741 cctggatccc agtatttcat tttcttgctg agcacaagag ctggtggcct gggcttaaat
1801 cttcaggcag ctgatacagt ggtcatcttt gacagcgact ggaatcctca tcaggatctg
1861 caggcccaag accgagctca ccgcatcggg cagcagaacg aggtccgggt actgaggctc
1921 tgtaccgtga acagcgtgga ggaaaagatc ctcgcggccg caaaatacaa gctgaacgtg
1981 gatcagaaag tgatccaggc gggcatgttt gaccaaaagt cttcaagcca cgagcggagg
2041 gcattcctgc aggccatctt ggagcatgag gaggaaaatg aggaagaaga tgaagtaccg
2101 gacgatgaga ctctgaacca aatgattgct cgacgagaag aagaatttga cctttttatg
2161 cggatggaca tggaccggcg gagggaagat gcccggaacc cgaaacggaa gccccgttta
2221 atggaggagg atgagctgcc ctcctggatc attaaggatg acgctgaagt agaaaggctc
2281 acctgtgaag aagaggagga gaaaatattt gggagggggt cccgccagcg ccgtgacgtg
2341 gactacagtg acgccctcac ggagaagcag tggctaaggg ccatcgaaga cggcaatttg
2401 gaggaaatgg aagaggaagt acggcttaag aagcgaaaaa gacgaagaaa tgtggataaa
2461 gatcctgcaa aagaagatgt ggaaaaagct aagaagagaa gaggccgccc tcccgctgag
2521 aaactgtcac caaatccccc caaactgaca aagcagatga acgctatcat cgatactgtg
2581 ataaactaca aagataggtg taacgtggag aaggtgccca gtaattctca gttggaaata
2641 gaaggaaaca gttcagggcg acagctcagt gaagtcttca ttcagttacc ttcaaggaaa
2701 gaattaccag aatactatga attaattagg aagccagtgg atttcaaaaa aataaaggaa
2761 aggattcgta atcataagta ccggagccta ggcgacctgg agaaggatgt catgcttctc
2821 tgtcacaacg ctcagacgtt caacctggag ggatcccaga tctatgaaga ctccatcgtc
2881 ttacagtcag tgtttaagag tgcccggcag aaaattgcca aagaggaaga gagtgaggat
2941 gaaagcaatg aagaggagga agaggaagat gaagaagagt cagagtccga ggcaaaatca
3001 gtcaaggtga aaattaagct caataaaaaa gatgacaaag gccgggacaa agggaaaggc
3061 aagaaaaggc caaatcgagg aaaagccaaa cctgtagtga gcgattttga cagcgatgag
3121 gagcaggatg aacgtgaaca gtcagaagga agtgggacgg atgatgagtg tacagactac
3181 aaagaccatg acggtgatta taaagatcat gacatcgact acaaggatga cgatgacaag
3241 taactcgaga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
3301 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
3361 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca
3421 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
3481 ctgacaattc cgtggtgttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
3541 tctagcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
3601 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
3661 tgggaggttt tttaaagatc tggagaccgg tgtggcacgt gcggaccgag cggccgcagg
3721 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
3781 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
3841 cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
3901 tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg cattaagcgc
3961 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct tagcgcccgc
4021 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
4081 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
4141 acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
4201 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
4261 caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt cggtctattg
4321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
4381 tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagcc
4441 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
4501 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
4561 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
4621 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
4681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
4741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
4801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
4861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
4921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
4981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
5041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
5101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
5161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
5221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
5281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
5341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
5401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
5461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
5521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
5581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
5641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
5701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
5761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
5821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
5881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
5941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
6001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
6061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
6121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
6181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
6241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
6301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
6361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
6421 gttcctggcc ttttgctggc cttttgctca catgt
//
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