| 质粒名称: | pCS2FLAG |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 16331 |
| 存储实验室: | Peter Klein |
|
载体骨架基本信息 |
|
| 载体名称: | pCS2+ |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 4095 bp |
| 完整质粒大小: | 4158 bp |
| 载体修饰: | 人细胞色素c氧化酶亚基VIII（human cytochrome C oxidase subunit VIII） |
| 载体类型: | 脊椎动物表达 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Flag Tag |
| 插入大小: | 63 bp |
| 基因突变: | Flag tag inserted into pCS2+ polylinker |
| 标签/融合蛋白: | Flag |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Cla I(未破坏) |
| 3' 克隆位点: | EcoR I (未破坏) |
| 5' 测序引物 | SP6 |
| 3' 测序引物 | T3 |
|
文献引用方法 |
|
| 材料和方法部分: | pCS2FLAG was a gift from Peter Klein (爱迪基因 plasmid # 16331) |
| 参考文献部分: | Klein lab unpublished plasmids (unpublished) |
买家导航
pCS2FLAG载体质粒基本信息
pCS2FLAG质粒图谱载体图谱和pCS2FLAG载体序列质粒序列多克隆位点信息
pCS2FLAG质粒载体简介
pCS2FLAG质粒序列载体序列
LOCUS pCS2FLAG 4151 bp ds-DNA circular SYN 10-JAN-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCS2FLAG
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4151)
TITLE Klein lab unpublished plasmids
REFERENCE 2 (bases 1 to 4151)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jan 10, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTNAME|pCS2FLAG|
FEATURES Location/Qualifiers
source 1..4151
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 35..53
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 35..52
/label=SP6
/note="SP6 promoter, forward primer"
CDS 103..150
/codon_start=1
/product="two tandem FLAG(R) epitope tags"
/label=2xFLAG
/translation="DYKDDDDKDYKDDDDK"
promoter complement(178..195)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 200..334
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(224..243)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 278..297
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind complement(447..467)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(447..465)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(486..502)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(486..502)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(499..521)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 510..526
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(534..564)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 579..600
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(717..734)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(888..1476)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(968..987)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1647..2507)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
primer_bind 2270..2289
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2508..2612)
/gene="bla"
/label=AmpR promoter
rep_origin complement(2638..3093)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(2775..2796)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2987..3006
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 3167..4151
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
ORIGIN
1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
61 ctacttgttc tttttgcagg atcccatcga tttaaagcta tggattacaa ggatgatgat
121 gataaggatt acaaggatga tgatgataag aattcaaggc ctctcgagcc tctagaacta
181 tagtgagtcg tattacgtag atccagacat gataagatac attgatgagt ttggacaaac
241 cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt
301 atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca ttcattttat
361 gtttcaggtt cagggggagg tgtgggaggt tttttaattc gcggccgcgg cgccaatgca
421 ttgggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
481 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
541 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
601 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
661 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
721 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
781 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
841 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
1021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
1081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
1141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
1201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
1261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
1321 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
1381 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
1441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
1501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
1561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
1621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
1681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
1741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
1801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
1861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
1921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
1981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
2041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
2101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
2161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
2221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
2281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
2341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
2401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
2461 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
2521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
2581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa
2641 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
2701 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
2761 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
2821 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
2881 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
2941 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
3001 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
3061 ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg ctgcgcaact
3121 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagtcgacc atagccaatt
3181 caatatggcg tatatggact catgccaatt caatatggtg gatctggacc tgtgccaatt
3241 caatatggcg tatatggact cgtgccaatt caatatggtg gatctggacc ccagccaatt
3301 caatatggcg gacttggcac catgccaatt caatatggcg gacttggcac tgtgccaact
3361 ggggaggggt ctacttggca cggtgccaag tttgaggagg ggtcttggcc ctgtgccaag
3421 tccgccatat tgaattggca tggtgccaat aatggcggcc atattggcta tatgccagga
3481 tcaatatata ggcaatatcc aatatggccc tatgccaata tggctattgg ccaggttcaa
3541 tactatgtat tggccctatg ccatatagta ttccatatat gggttttcct attgacgtag
3601 atagcccctc ccaatgggcg gtcccatata ccatatatgg ggcttcctaa taccgcccat
3661 agccactccc ccattgacgt caatggtctc tatatatggt ctttcctatt gacgtcatat
3721 gggcggtcct attgacgtat atggcgcctc ccccattgac gtcaattacg gtaaatggcc
3781 cgcctggctc aatgcccatt gacgtcaata ggaccaccca ccattgacgt caatgggatg
3841 gctcattgcc cattcatatc cgttctcacg ccccctattg acgtcaatga cggtaaatgg
3901 cccacttggc agtacatcaa tatctattaa tagtaacttg gcaagtacat tactattgga
3961 aggacgccag ggtacattgg cagtactccc attgacgtca atggcggtaa atggcccgcg
4021 atggctgcca agtacatccc cattgacgtc aatggggagg ggcaatgacg caaatgggcg
4081 ttccattgac gtaaatgggc ggtaggcgtg cctaatggga ggtctatata agcaatgctc
4141 gtttagggaa c
//
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pCS2FLAG质粒载体应用举例
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