| 质粒名称: | pAAV-hSyn-GRAB_Ado1.0med |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000126 |
| 产品用途: | Express the medium-affinity adenosine sensor GRAB_Ado1.0med in neurons |
| 存储实验室: | Yulong Li Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | hSyn |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | GRAB_Ado1.0med |
| 物种来源: | Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Neuronal activity-induced, equilibrative nucleoside transporter-dependent, somatodendritic adenosine release revealed by a GRAB sensor
Wu Z., Cui Y., Wang H., Song K., Yuan Z., Dong A., Wu H., Wan Y., Pan S., Peng W., Jing M., Xu M., Luo M., Li Y.
Proc. Natl. Acad. Sci. U. S. A. 120, e2212387120 (2023)
|
买家导航
pAAV-hSyn-GRAB_Ado1.0med载体质粒基本信息
pAAV-hSyn-GRAB_Ado1.0med质粒图谱载体图谱和pAAV-hSyn-GRAB_Ado1.0med载体序列质粒序列多克隆位点信息
pAAV-hSyn-GRAB_Ado1.0med质粒载体简介
pAAV-hSyn-GRAB_Ado1.0med质粒序列载体序列
LOCUS pAAV-hSyn-GRAB_Ado1.0med 6740 bp DNA circular SYN 21-APR-2023
DEFINITION Express the medium-affinity adenosine sensor GRAB_Ado1.0med in
neurons.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6740)
AUTHORS Zhaofa Wu, Yuting Cui, Huan Wang, Kun Song, Zhengwei Yuan, Ao Dong,
Hao Wu, Yi Wan, Sunlei Pan, Wanling Peng, Miao Jing, Min Xu, Minmin
Luo, Yulong Li
TITLE A GRAB sensor reveals activity-dependent non-vesicular
somatodendritic adenosine release
JOURNAL bioRxiv
REFERENCE 2 (bases 1 to 6740)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 27, 2026 from SnapGene 6.0.2
https:///www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv"
FEATURES Location/Qualifiers
source 1..6740
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR (alternate)
/note="Functional equivalent of wild-type AAV2 ITR"
promoter 131..574
/label=hSyn
regulatory 581..590
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
sig_peptide 587..649
/codon_start=1
/product="leader sequence from mouse immunoglobulin kappa
light chain"
/label=Ig-kappa leader
/translation="METDTLLLWVLLLWVPGSTGD"
protein_bind 650..674
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 674..2842
/codon_start=1
/label=GRAB_Ado1.0med
/translation="TGPIMGSSVYITVELAIAVLAILGNVLVCWAVWLNSNLQNVTNYF
VVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRY
IAIRIPLRYNGLVTGTRAKGIIAICWVLSFAIGLTPMLGWNNCGQPKEGKNHSQGCGEG
QVACLFEDVVPMNYMVYFNFFACVLVPLLLMLGVYLRIFLAARRQLKQMESQPPSRRGP
DAVAAPPGGTERRPNGLGPERSAGPGGAEAEPLPTQLNGAPGEPAPAGPRDTDALDLEE
GGNVYIKADKQKNGIKANFHIRHNIEDGGVQLAYHYQQNTPIGDGPVLLPDNHYLSVQS
KLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVRKGEELFTGVVPILVELD
GDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHM
KQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNIL
GHKLEYNTGAAARWRGRQNRELHAAKSLAIIVGLFALCWLPLHIINCFTFFCPDCSHAP
LWLHYLAIVLSHTNSVVNPFIYAYRIREFRQTFRKIIRSHVLRQQEPFKAAGTSARVLA
AHGSDGEQVSLRLNGHPPGVWANGSAPHPERRPNGYALGLVSGGSAQESQGNTGLPDVE
LLSHELKGVCPEPPGLDDPLAQDGAGVS"
primer_bind 1259..1280
/label=WKG125-F
primer_bind 1729..1750
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind complement(1856..1877)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(2117..2136)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
misc_feature 2867..3455
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2920..2940)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3338..3349)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3487..3963
/label=hGH poly(A) signal
/note="human growth hormone polyadenylation signal"
primer_bind complement(3686..3705)
/label=hGH-PA-R
/note="Human growth hormone terminator, reverse primer"
repeat_region 4003..4143
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 4003..4132
/label=AAV2 ITR
rep_origin 4218..4673
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(4305..4324)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 4515..4536
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(4690..4709)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4809..4831
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(4869..4887)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 4955..5059
/gene="bla"
/label=AmpR promoter
CDS 5060..5920
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5278..5297)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6091..6679
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6580..6599
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtatgagg cggggtgggg gtgcctacct gacgaccgac
181 cccgacccac tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag
241 agggggaggg gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg
301 acagtgcctt cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg
361 ctgacgtcac tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc
421 gccgccgccg gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc
481 gaccatctgc gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga
541 ggagtcgtgt cgtgcctgag agcgcagtcg agaaaccggt gccaccatgg agacagacac
601 actcctgcta tgggtactgc tgctctgggt tccaggttcc actggtgaca caagtttgta
661 caaaaaagtt ggcaccggtc ccatcatggg ctcctcggtg tacatcacgg tggagctggc
721 cattgctgtg ctggccatcc tgggcaatgt gctggtgtgc tgggccgtgt ggctcaacag
781 caacctgcag aacgtcacca actactttgt ggtgtcactg gcggcggccg acatcgcagt
841 gggtgtgctc gccatcccct ttgccatcac catcagcacc gggttctgcg ctgcctgcca
901 cggctgcctc ttcattgcct gcttcgtcct ggtcctcacg cagagctcca tcttcagtct
961 cctggccatc gccattgacc gctacattgc catccgcatc ccgctccggt acaatggctt
1021 ggtgaccggc acgagggcta agggcatcat tgccatctgc tgggtgctgt cgtttgccat
1081 cggcctgact cccatgctag gttggaacaa ctgcggtcag ccaaaggagg gcaagaacca
1141 ctcccagggc tgcggggagg gccaagtggc ctgtctcttt gaggatgtgg tccccatgaa
1201 ctacatggtg tacttcaact tctttgcctg tgtgctggtg cccctgctgc tcatgctggg
1261 tgtctatttg cggatcttcc tggcggcgcg acgacagctg aagcagatgg agagccagcc
1321 tcccagccgc cggggtccgg acgccgtcgc cgcgccgccg gggggcaccg agcgcaggcc
1381 caacggtctg ggccccgagc gcagcgcggg cccggggggc gcagaggccg aaccgctgcc
1441 cacccagctc aacggcgccc ctggcgagcc cgcgccggcc gggccgcgcg acaccgacgc
1501 gctggacctg gaggagggag gaaacgtcta tatcaaggcc gacaagcaga agaacggcat
1561 caaggcgaac ttccacatcc gccacaacat cgaggacggc ggcgtgcagc tcgcctacca
1621 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
1681 gagcgtgcag tccaaacttt cgaaagaccc caacgagaag cgcgatcaca tggtcctgct
1741 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
1801 cggagggagc atggtgagaa agggcgagga gctgttcacc ggggtggtgc ccatcctggt
1861 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gtgagggcga
1921 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
1981 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
2041 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctaca tccaggagcg
2101 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
2161 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
2221 cctggggcac aagctggagt acaacaccgg agcagcagca cgctggcgcg ggcggcagaa
2281 ccgcgagctc catgctgcca agtcactggc catcattgtg gggctctttg ccctctgctg
2341 gctgccccta cacatcatca actgcttcac tttcttctgc cccgactgca gccacgcccc
2401 tctctggctc cactacctgg ccatcgtcct ctcccacacc aattcggttg tgaatccctt
2461 catctacgcc taccgtatcc gcgagttccg ccagaccttc cgcaagatca ttcgcagcca
2521 cgtcctgagg cagcaagaac ctttcaaggc agctggcacc agtgcccggg tcttggcagc
2581 tcatggcagt gacggagagc aggtcagcct ccgtctcaac ggccacccgc caggagtgtg
2641 ggccaacggc agtgctcccc accctgagcg gaggcccaat ggctacgccc tggggctggt
2701 gagtggaggg agtgcccaag agtcccaggg gaacacgggc ctcccagacg tggagctcct
2761 tagccatgag ctcaagggag tgtgcccaga gccccctggc ctagatgacc ccctggccca
2821 ggatggagca ggagtgtcct gagaattcga tatcaagctt atcgataatc aacctctgga
2881 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
2941 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
3001 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
3061 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
3121 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
3181 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
3241 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct atgttgccac
3301 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
3361 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
3421 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgagcgc tgctcgagag
3481 atctacgggt ggcatccctg tgacccctcc ccagtgcctc tcctggccct ggaagttgcc
3541 actccagtgc ccaccagcct tgtcctaata aaattaagtt gcatcatttt gtctgactag
3601 gtgtccttct ataatattat ggggtggagg ggggtggtat ggagcaaggg gcaagttggg
3661 aagacaacct gtagggcctg cggggtctat tgggaaccaa gctggagtgc agtggcacaa
3721 tcttggctca ctgcaatctc cgcctcctgg gttcaagcga ttctcctgcc tcagcctccc
3781 gagttgttgg gattccaggc atgcatgacc aggctcagct aatttttgtt tttttggtag
3841 agacggggtt tcaccatatt ggccaggctg gtctccaact cctaatctca ggtgatctac
3901 ccaccttggc ctcccaaatt gctgggatta caggcgtgaa ccactgctcc cttccctgtc
3961 cttctgattt tgtaggtaac cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg
4021 agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg
4081 cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc
4141 aggggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
4201 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
4261 tacgcgcagc gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt
4321 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
4381 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
4441 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
4501 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg
4561 ctattctttt gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct
4621 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
4681 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
4741 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
4801 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
4861 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
4921 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
4981 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
5041 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
5101 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
5161 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
5221 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
5281 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
5341 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
5401 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
5461 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
5521 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
5581 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
5641 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
5701 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
5761 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
5821 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
5881 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
5941 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
6001 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
6061 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
6121 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
6181 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
6241 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
6301 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
6361 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
6421 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
6481 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
6541 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
6601 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
6661 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
6721 ctggcctttt gctcacatgt
//
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