| 质粒名称: | pGEX-6p-1-Caprin1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000944 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-6p-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | Caprin1 |
| 物种来源: | human |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-6p-1-Caprin1载体质粒基本信息
pGEX-6p-1-Caprin1质粒图谱载体图谱和pGEX-6p-1-Caprin1载体序列质粒序列多克隆位点信息
pGEX-6p-1-Caprin1质粒载体简介
pGEX-6p-1-Caprin1质粒序列载体序列
LOCUS pGEX-6p-1-Caprin1 7120 bp DNA circular SYN 10-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7120)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 17, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7120
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
CDS 951..971
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 978..3104
/codon_start=1
/label=human Caprin1 codon
/translation="MPSATSHSGSGSKSSGPPPPSGSSGSEAAAGAGAAAPASQHPATG
TGAVQTEAMKQILGVIDKKLRNLEKKKGKLDDYQERMNKGERLNQDQLDAVSKYQEVTN
NLEFAKELQRSFMALSQDIQKTIKKTARREQLMREEAEQKRLKTVLELQYVLDKLGDDE
VRTDLKQGLNGVPILSEEELSLLDEFYKLVDPERDMSLRLNEQYEHASIHLWDLLEGKE
KPVCGTTYKVLKEIVERVFQSNYFDSTHNHQNGLCEEEEAASAPAVEDQVPEAEPEPAE
EYTEQSEVESTEYVNRQFMAETQFTSGEKEQVDEWTVETVEVVNSLQQQPQAASPSVPE
PHSLTPVAQADPLVRRQRVQDLMAQMQGPYNFIQDSMLDFENQTLDPAIVSAQPMNPTQ
NMDMPQLVCPPVHSESRLAQPNQVPVQPEATQVPLVSSTSEGYTASQPLYQPSHATEQR
PQKEPIDQIQATISLNTDQTTASSSLPAASQPQVFQAGTSKPLHSSGINVNAAPFQSMQ
TVFNMNAPVPPVNEPETLKQQNQYQASYNQSFSSQPHQVEQTELQQEQLQTVVGTYHGS
PDQSHQVTGNHQQPPQQNTGFPRSNQPYYNSRGVSRGGSRGARGLMNGYRGPANGFRGG
YDGYRPSFSNTPNSGYTQSQFSAPRDYSGYQRDGYQQNFKRGSGQSGPRGAPRGRGGPP
RPNRGMPQMNTQQVN"
promoter 3423..3527
/gene="bla"
/label=AmpR promoter
CDS 3528..4388
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4559..5147
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 5391..5468
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 5469..6551
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 6564..6585
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6600..6630
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6638..6654
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6662..6678
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS join(6674..7120,1..81)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGITLSTARCTNASGVR
QPSEAVVWLCRS"
primer_bind complement(6694..6710)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc gaaaacctgt
961 atttccaggg cggtagtatg ccgagtgcca ccagccatag cggtagcggt agcaaaagta
1021 gtggtccgcc gccgccgagc ggtagctcag gttcagaagc cgcagccggt gccggtgcag
1081 ccgcacctgc aagtcagcat ccggcaaccg gtacaggtgc cgttcagacc gaagcaatga
1141 aacagattct gggcgttatt gataaaaaac tgcgcaatct ggaaaagaaa aaaggtaaac
1201 tggatgatta ccaggaacgc atgaataagg gcgaacgtct gaatcaggat cagctggatg
1261 ccgtgagtaa atatcaggaa gttaccaata atctggaatt tgccaaagaa ctgcagcgta
1321 gttttatggc cctgagccag gatattcaga aaaccattaa gaaaaccgca cgccgtgaac
1381 agctgatgcg tgaagaagcc gaacagaaac gtctgaaaac cgttctggaa ctgcagtatg
1441 tgctggataa actgggtgac gatgaagttc gtaccgatct gaaacagggt ctgaatggtg
1501 tgccgattct gagtgaagaa gaactgagcc tgctggatga attttataaa ctggtggacc
1561 ctgaacgtga tatgagcctg cgtctgaatg aacagtatga acatgccagc attcatctgt
1621 gggatctgct ggaaggtaaa gaaaaaccgg tttgtggcac cacctataaa gttctgaaag
1681 aaattgtgga acgtgttttt cagagtaatt attttgacag cacccataat catcagaatg
1741 gcctgtgtga agaagaagaa gcagcaagtg ccccggccgt ggaagatcag gttccggaag
1801 cagaaccgga accggccgaa gaatataccg aacagagcga agtggaaagt accgaatatg
1861 tgaatcgcca gtttatggcc gaaacccagt ttaccagtgg cgaaaaagaa caggtggatg
1921 aatggaccgt tgaaaccgtt gaagttgtga atagcctgca gcagcagccg caggcagcaa
1981 gcccgagcgt gcctgaaccg catagtctga ccccggttgc acaggcagat ccgctggtgc
2041 gccgccagcg tgttcaggat ctgatggcac agatgcaggg cccgtataat tttattcagg
2101 atagcatgct ggattttgaa aatcagaccc tggaccctgc cattgttagc gcccagccga
2161 tgaatccgac ccagaatatg gatatgccgc agctggtgtg cccgccggtg catagtgaaa
2221 gtcgtctggc ccagccgaat caggttccgg ttcagccgga agcaacccag gtgccgctgg
2281 ttagtagtac cagcgaaggt tataccgcaa gtcagccgct gtatcagccg agccatgcca
2341 ccgaacagcg tccgcagaaa gaaccgattg atcagattca ggccaccatt agtctgaata
2401 ccgatcagac caccgccagt agcagcctgc cggccgctag tcagccgcag gtttttcagg
2461 caggcaccag caaaccgctg catagtagcg gtattaatgt taatgcagca ccgtttcaga
2521 gtatgcagac cgtgtttaat atgaatgccc cggttccgcc ggtgaatgaa ccggaaaccc
2581 tgaaacagca gaatcagtat caggccagct ataatcagag ttttagtagc cagccgcatc
2641 aggttgaaca gaccgaactg cagcaggaac agctgcagac cgtggtgggc acctatcatg
2701 gtagtccgga tcagagtcat caggtgaccg gcaatcatca gcagccgccg cagcagaata
2761 ccggctttcc gcgcagcaat cagccgtatt ataatagccg tggcgtgagc cgtggtggta
2821 gccgcggtgc acgtggtctg atgaatggtt atcgtggccc ggcaaatggc tttcgtggtg
2881 gttatgatgg ctatcgtccg agctttagta ataccccgaa tagtggttat acccagagcc
2941 agtttagcgc accgcgtgat tatagcggct atcagcgcga tggttatcag cagaatttta
3001 aacgcggcag cggtcagagc ggtccgcgcg gtgcacctcg tggtcgtggt ggtccgccgc
3061 gtcctaatcg cggcatgccg cagatgaata cccagcaggt gaatctcgag cggccgcatc
3121 gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
3181 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
3241 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
3301 gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc ctatttttat
3361 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
3421 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
3481 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
3541 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
3601 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
3661 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
3721 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
3781 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
3841 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
3901 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
3961 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
4021 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg
4081 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
4141 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
4201 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
4261 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
4321 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
4381 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
4441 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
4501 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
4561 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
4621 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
4681 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
4741 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
4801 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
4861 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
4921 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
4981 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
5041 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
5101 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
5161 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
5221 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
5281 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
5341 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataaattcc gacaccatcg
5401 aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt caattcaggg
5461 tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc
5521 agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag
5581 tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg
5641 gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc
5701 aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga
5761 tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac
5821 gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag
5881 ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca
5941 gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg
6001 gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc
6061 tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag
6121 gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg
6181 ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta
6241 ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag
6301 acagctcatg ttatatcccg ccgtcaacca ccatcaaaca ggattttcgc ctgctggggc
6361 aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc
6421 tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct
6481 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
6541 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
6601 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
6661 acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac aacgtcgtga
6721 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
6781 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
6841 tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga
6901 gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta
6961 cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc cgtttgttcc
7021 cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa gctggctaca
7081 ggaaggccag acgcgaatta tttttgatgg cgttggaatt
//
pGEX-6p-1-Caprin1载体图谱质粒图谱pdf版和pGEX-6p-1-Caprin1载体序列质粒序列等相关资料下载
pGEX-6p-1-Caprin1质粒载体应用举例
- 上一篇：pGEX-2T-G3BP1-Flag
- 下一篇：没有了
