| 质粒名称: | pAAV-CAG-UnaG-P2A-FusionRed |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000230 |
| 产品用途: | Expresses UnaG and FusionRed in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | UnaG, FusionRed |
| 物种来源: | Anguilla japonica |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Enhanced small green fluorescent proteins as a multisensing platform for biosensor development
Liang G., Lai C., Yue Z., Zhang H., Li D., Chen Z., Lu X., Tao L., Subach F.V., Piatkevich K.D.
Front. Bioeng. Biotechnol. 10, 1039317 (2022)
|
pAAV-CAG-UnaG-P2A-FusionRed
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-UnaG-P2A-FusionRed载体质粒基本信息
pAAV-CAG-UnaG-P2A-FusionRed质粒图谱载体图谱和pAAV-CAG-UnaG-P2A-FusionRed载体序列质粒序列多克隆位点信息
pAAV-CAG-UnaG-P2A-FusionRed质粒载体简介
pAAV-CAG-UnaG-P2A-FusionRed质粒序列载体序列
LOCUS pAAV-CAG-UnaG-P2A-FusionRed 5802 bp DNA circular SYN 26-MAY-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5802)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jul 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5802
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
misc_feature 977..986
/label=Kozak sequence
CDS 983..1399
/codon_start=1
/label=UnaG
/translation="MVEKFVGTWKIADSHNFGEYLKAIGAPKELSDGGDATTPTLYISQ
KDGDKMTVKIENGPPTFLDTQVKFKLGEEFDEFPSDRRKGVKSVVNLVGEKLVYVQKWD
GKETTYVREIKDGKLVVTLTMGDVVAVRSYRRATE"
CDS 1424..1480
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1493..2188
/codon_start=1
/product="monomeric red fluorescent protein optimized for
use as a fusion tag (Shemiakina et al., 2012)"
/label=FusionRed
/note="mammalian codon-optimized"
/translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
STGGAGDGGK"
misc_feature 2216..2804
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2687..2698)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2847..2968
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3366..3954)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4125..4985)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4986..5090)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5116..5571)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
repeat_region 5643..5772
/label=AAV2 ITR
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggtcga gaaattcgtg gggacttgga aaatcgcaga
1021 ctcacataac ttcggagaat acctgaaagc aatcggggca cctaaagagc tgtctgacgg
1081 cggggatgcc accacaccaa ctctgtacat cagtcagaag gacggcgata aaatgaccgt
1141 gaagattgag aacgggcccc ctaccttcct ggacacacag gtgaagttta aactgggcga
1201 ggagttcgac gagtttccca gcgatcggag aaagggagtg aaatccgtgg tcaatctggt
1261 cggcgaaaaa ctggtgtacg tccagaaatg ggatggcaag gagactacct atgtgcgaga
1321 aatcaaggac gggaaactgg tggtcacact gactatggga gatgtggtcg ccgtgagatc
1381 ctataggcgc gctacagaac gaccggttca cgtgggaagt ggggctacaa acttctcact
1441 gctgaaacag gccggggatg tggaggaaaa cccaggacca cacgtgacta gtatggtgag
1501 cgagctgatt aaggagaaca tgcccatgaa gctgtacatg gagggcaccg tgaacaacca
1561 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
1621 aatcaaggtc gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
1681 catgtacggc agcagaacct tcatcaagca ccctccgggc atccccgact tctttaagca
1741 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
1801 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaaggttag
1861 aggggtgaac ttcccagcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
1921 ctccaccgag acgatgtacc ctgctgacgg aggactggaa ggcgcatgtg acatggctct
1981 gaagctcgtg ggaggaggac acctgatctg caaccttgag acaacataca gatccaagaa
2041 acccgctacg aacctcaaga tgcccggcgt ctacaacgtg gaccacagac tggaaagaat
2101 caaggaggcc gacgatgaga catacgtcga gcagcacgag gtggctgtgg ccagatactc
2161 tactggtggc gctggtgatg gaggtaaata agaattcgat atcaagctta tcgataatca
2221 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
2281 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
2341 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
2401 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
2461 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
2521 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
2581 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
2641 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
2701 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
2761 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
2821 cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
2881 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
2941 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
3001 ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
3061 aatcgataag gatcttccta gagcatggct acgtagataa gtagcatggc gggttaatca
3121 ttaactacac tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
3181 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3241 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3301 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3541 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
3721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
3781 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
3841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
3901 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
3961 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
4021 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4081 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4141 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4201 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4261 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4321 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4381 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4441 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4501 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4561 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4621 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4681 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4741 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4801 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
4861 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
4921 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
4981 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
5041 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5101 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
5161 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
5221 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
5281 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
5341 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
5401 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
5461 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
5521 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
5581 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
5641 agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct
5701 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
5761 ctaggggttc cttgtagtta atgattaacc cgccatgcta ct
//
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