| 质粒名称: | pCE35 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 174409 |
| 存储实验室: | Clarissa Nobile |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Clontech |
| 空载体大小: | 2242bp |
| 完整质粒大小: | 9204bp |
| 载体类型: | Bacterial Expression, CRISPR |
| 筛选标记: | Nourseothricin (NAT) |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | C. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2 |
| 物种来源: | Candida auris |
| 插入大小: | 6962bp |
| 克隆方法: | Ligation Independent Cloning |
| 5’测序引物 | cacatttccccgaaaagtg |
| 3’测序引物 |
CTCACTGCCCGCTTTCCAGT
(Common Sequencing Primers)
|
|
文献引用方法 |
|
| 材料和方法部分: | pCE35 was a gift from Clarissa Nobile (Addgene plasmid # 174409 ; http://n2t.net/addgene:174409 ; RRID:Addgene_174409) |
| 参考文献部分: | A Markerless CRISPR-Mediated System for Genome Editing in Candida auris Reveals a Conserved Role for Cas5 in the Caspofungin Response. Ennis CL, Hernday AD, Nobile CJ. Microbiol Spectr. 2021 Dec 22;9(3):e0182021. doi: 10.1128/Spectrum.01820-21. Epub 2021 Nov 3. 10.1128/Spectrum.01820-21 PubMed 34730409 |
买家导航
pCE35载体质粒基本信息
pCE35质粒图谱载体图谱和pCE35载体序列质粒序列多克隆位点信息
pCE35质粒载体简介
pCE35质粒序列载体序列
LOCUS pCE35 9205 bp DNA circular SYN 14-APR-2025
DEFINITION C. auris LEUpOUT NAT marker CAS9 expression construct. Use with
pCE27 gRNA expression construct..
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9205)
AUTHORS Ennis CL, Hernday AD, Nobile CJ
TITLE A Markerless CRISPR-Mediated System for Genome Editing in Candida
auris Reveals a Conserved Role for Cas5 in the Caspofungin Response.
JOURNAL Microbiol Spectr. 2021 Dec 22;9(3):e0182021. doi:
10.1128/Spectrum.01820-21. Epub 2021 Nov 3.
PUBMED 34730409
REFERENCE 2 (bases 1 to 9205)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 14, 2025 from SnapGene Server 8.0.1
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Microbiol
Spectr."; date: "2021-12-22"; volume: "9(3):e0182021. doi"; pages: "
10.1128/Spectrum.01820-21. Epub 2021 Nov 3"
COMMENT Sequence Label: pCE35
FEATURES Location/Qualifiers
source 1..9205
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter complement(184..288)
/gene="bla"
/label=AmpR promoter
primer_bind 356..374
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
CDS 1962..6065
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 1962..6065
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 6078..6098
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6102..6122
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6126..6146
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 6147..6212
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 6277..6524
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(6293..6311)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
promoter 6564..6907
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
promoter complement(join(7415..7421,7422..7439,7440..7445))
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7460..7481
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7598..7615)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7769..8357)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7849..7868)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(join(8528..9205,1..114,115..183))
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9151..9170
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
61 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
121 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
181 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
241 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
301 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
361 gcgtatcacg aggccctttg acggcacggc cacgcgttta aaccgccttt tcaaatggtt
421 cagaaaaata ttgttcttct tcctggtgac cacgtgggca ccgaggttgt aaacgaggcc
481 gtcaaggttt tgaaagccat tgaggaagca acgtctcaag atatcaaatt caatttttca
541 acccatctaa ttggtggtgc agccattgac aagaccggag tgcctttgcc cgatgaagcc
601 ctcgaggctg ccaaaaagtc agacgctgtc ttgttgggag ccgttggtgg ccccaaatgg
661 ggtactggtg ccgttagacc agagcagggt cttctcaaga tcagaaagga attgggcctt
721 tatgcgaact tgagaccttg caactttgca tctgactcgc ttttggacct ttctccattg
781 aaggccagtg ttgtcaaagg aaccaatttc accgtggtgc gtgaattggt cggtggtatc
841 tactttggtg agagacaaga acaggaagag tctgccgacg gtcagagcgc ttgggacacc
901 gagaagtaca gtgtctctga agttgaaaga atcaccagaa tggctgcctt catggctttg
961 cagcacaacc ctcctttgcc catctggtcc ttagacaagg ccaacgtgct tgcctcatct
1021 agattgtgga gaaagacggt cgacaaggtg atgagcgagg agttccctca attgaccatc
1081 aaccaccagc tcattgactc tgctgccatg attttggtgc agagcccaac taaattgaac
1141 ggtgttgtga tcacgtcaaa catgaccaat cacaagactt acctgaagac ctgactcaac
1201 gcaatcgcca gtcccgggtt tcctggctca cgggcccctc cactacgctg atattttgtc
1261 aattaaccag gagtctaccc tagtgcgcat tgtcgaaggg agtggaaagc cagggaaaaa
1321 aaacgtatag tgcggtcccc gtcgacccga gacgacaagg cacccaggtg agtcaggctc
1381 aaatgcatga aaaattatga aaagtggaaa atcgatggcc ctcaggtgaa ggttatcaaa
1441 acctcatcag ctcgtcgctc aattgctaaa aatcaactga aacagcgtcc cagtggtgtc
1501 accccacggt tgtttcaggg ctgcggtgga tcacatggcg actccctttg gcccgtaggg
1561 tgcgatccgt cagtgcccgg ggccctctgc caggattcta cgcgcattgc aactccgagg
1621 ccgataccct ttgaccagtg gtcaattcgt cacaattggc aacattttca tgatttggca
1681 gcttcacgtt gaacaattga accagcagaa tccaggaaac gagtttccgt tgtgaatgac
1741 cctttgtgtg tgcaaataat catggtggct ccctaatggc ataccctcag tcacctggag
1801 gaattgctcg ctcgagtctc ctgaggtgtc ctcctgcctg tggaaaaagc ggccaggtgt
1861 ggtccaccgt tggaacagct tgctgtataa ataccacagg tgcgcacgcc ccaggggcct
1921 tttttttttt ttctcctccc tatccaaact taattttcat catggataaa aagtatagta
1981 ttggtttaga tattggtact aactctgtgg gttgggcagt tatcactgac gaatataaag
2041 ttccatcaaa gaaatttaag gtgttaggta acactgacag acactcaata aaaaagaatc
2101 ttatcggtgc tcttttgttc gactccggtg aaactgccga ggctacacgt ttaaaaagaa
2161 cagcaagaag aagatatacc cgtagaaaaa atagaatatg ttatttacaa gaaatctttt
2221 ctaatgaaat ggctaaagtt gatgattcct ttttccatag attggaagag tcatttttgg
2281 ttgaagaaga caaaaagcat gagagacatc caatctttgg gaatatagtt gatgaagtgg
2341 cttaccatga aaaatatcct accatttatc atttaagaaa gaaattggta gattcaactg
2401 ataaagctga ccttagatta atctatttag cacttgccca tatgattaaa tttagaggtc
2461 attttttgat tgaaggtgat ttgaacccag ataattctga cgtggataaa ttatttattc
2521 aattagtcca aacctacaac caattatttg aggaaaatcc aattaatgct agtggtgtcg
2581 atgccaaagc tatattatca gccagattat caaaatctag acgtttggaa aatttgattg
2641 cccaattgcc aggagaaaaa aagaatggat tatttggaaa cttgatcgca ttatcattgg
2701 gtttgacacc aaattttaaa tctaattttg atttagctga agatgctaaa ttacaattat
2761 caaaagacac ctatgacgac gatttggaca atttacttgc tcaaattggt gatcaatatg
2821 cagatttgtt cttagctgct aaaaacttat ctgatgctat tttgttgtct gatattttga
2881 gagtgaacac agaaataacc aaagctccat tatcagcatc tatgatcaaa cgttatgatg
2941 aacaccatca ggatttgact ttattgaaag ctttggtgag acaacaattg ccagagaagt
3001 ataaagaaat ctttttcgat caatctaaaa acgggtatgc aggttatatt gatgggggtg
3061 cctcccaaga ggaattttac aaatttataa aacctatttt agaaaagatg gatgggactg
3121 aggaactttt ggtcaaattg aacagagaag atttgttacg taaacagaga acttttgata
3181 atggtagtat acctcaccaa attcatttgg gtgagttgca tgcaatttta agaagacaag
3241 aagattttta tccattttta aaagataata gagaaaaaat cgagaaaatt ttaaccttta
3301 gaattccata ctatgttggg cctttggcta gaggtaattc aagatttgcc tggatgacac
3361 gtaaatcaga agaaactatt accccttgga attttgaaga ggttgttgat aaaggagcat
3421 cagcacagag ttttattgaa agaatgacca atttcgataa aaacttacca aatgaaaaag
3481 ttttaccaaa acattccttg ttatacgaat attttactgt ttacaatgaa cttacaaagg
3541 ttaaatatgt tactgaaggt atgcgtaagc cagccttttt atctggagaa cagaaaaagg
3601 caatagttga tttattgttt aaaacaaata gaaaagttac tgttaaacaa ttaaaagaag
3661 attactttaa gaaaattgaa tgttttgatt cagttgaaat cagtggtgtt gaagacagat
3721 ttaatgctag tttaggaact taccatgatt tacttaaaat tatcaaagat aaagatttct
3781 tggataacga agaaaatgaa gacattttag aagacattgt tttaacctta actttattcg
3841 aagatagaga gatgattgaa gaacgtttga agacttatgc acatttgttt gacgataaag
3901 tgatgaaaca gttgaaaaga agacgttata ctggatgggg tagattgtct cgtaaattga
3961 tcaatggaat tagagataaa caaagtggta aaactatctt ggactttttg aaatctgacg
4021 gatttgctaa tagaaatttc atgcaattga tccacgacga tagtttgaca tttaaagaag
4081 acatccaaaa ggcccaagtg agtgggcaag gtgattcatt acatgaacat attgcaaatt
4141 tagccggatc tcctgctatt aagaaaggga tattacaaac tgttaaagtt gtggatgaat
4201 tagtgaaagt aatgggaaga cataaacctg aaaacattgt cattgagatg gcaagagaaa
4261 atcaaactac acaaaaagga cagaaaaata gtagagaacg tatgaaaaga atagaagagg
4321 gtattaaaga attgggtagt caaatattga aagaacaccc agtggaaaat acccagttgc
4381 aaaatgaaaa attatatctt tactaccttc aaaatggacg tgatatgtat gttgatcagg
4441 aattagatat aaatagactt tcagattatg atgtagatca tatagttcca caatctttct
4501 tgaaagatga ttccatagac aataaagtat taactagaag tgataaaaat agaggtaaaa
4561 gtgataatgt cccaagtgag gaagtcgtca aaaagatgaa aaattactgg cgtcaacttt
4621 tgaatgctaa attaattact caaagaaaat ttgataattt gactaaagca gaaagaggtg
4681 ggctttctga attagataaa gccgggttca ttaaaagaca attggtcgaa actagacaaa
4741 ttactaaaca tgttgcccaa attttagatt cccgtatgaa cactaagtat gacgaaaatg
4801 ataagttaat acgtgaggtt aaagtcatta ctttaaaatc aaaacttgtc tctgatttca
4861 gaaaggattt ccaattctat aaagttagag aaattaataa ttatcatcat gctcatgatg
4921 catatttgaa tgctgtagtt ggaactgctt taatcaagaa ataccctaaa ttagaatctg
4981 aatttgtata tggtgattac aaagtctatg atgttagaaa gatgattgct aaatcagaac
5041 aagaaattgg taaagctaca gctaaatact tcttttactc taacattatg aatttcttta
5101 aaacagaaat tactttggca aacggtgaaa ttagaaaaag acctcttatt gaaacaaatg
5161 gtgagactgg agagatagtt tgggacaaag ggcgtgattt cgctactgtt agaaaagttt
5221 tatcaatgcc acaagttaac attgtaaaga aaacagaggt tcaaactggt ggtttctcaa
5281 aagaaagtat tttgcctaaa agaaatagtg ataaattgat tgccagaaaa aaggattggg
5341 atccaaagaa atatggtggt ttcgactcac caaccgtagc ctattctgtt ttggttgtgg
5401 caaaggttga aaagggtaaa agtaaaaagc ttaaatcagt aaaagaactt ttgggtatta
5461 caataatgga aagaagttcc tttgaaaaga accctattga ttttttggaa gctaaaggtt
5521 ataaggaagt aaagaaggac ttaataatca aattgcctaa atattcttta tttgaattag
5581 aaaatgggag aaaaagaatg ttggcttctg ctggagaatt gcaaaagggt aatgaattag
5641 cattgccttc caaatatgtt aacttcttgt atttagcttc acactatgaa aagttgaaag
5701 ggtcaccaga agataacgag caaaaacaat tatttgttga acaacacaaa cactacttag
5761 atgagattat agaacaaatt agtgaattca gtaaaagagt gatattagct gatgcaaatt
5821 tagataaagt tttgtcagcc tataacaaac atagagataa gccaattaga gaacaagcag
5881 aaaacattat tcacttattt acccttacca atttaggagc acctgctgct ttcaagtatt
5941 ttgatacaac aattgatcgt aaaagatata cctcaacaaa agaagtctta gacgccacct
6001 taattcatca atcaatcact ggattgtatg agacaagaat tgatttgtct caattgggtg
6061 gtgatgaagg ggctgatcct aagaagaaaa gaaaagttga tccaaagaaa aagcgtaagg
6121 tggatcctaa gaaaaagaga aaggttgact acaaagacca tgacggtgat tataaagatc
6181 atgacatcga ctacaaggat gacgatgaca agtgataatg actgcagaga tccatcgacc
6241 tgccgccaag ctaattccgg gcgaatttct gtcgagtcat gtaattagtt atgtcacgct
6301 tacattcacg ccctcccccc acatccgctc taaccgaaaa ggaaggagtt agacaacctg
6361 aagtctaggt ccctatttat ttttttatag ttatgttagt attaagaacg ttatttatat
6421 ttcaaatttt tctttttttt ctgtacagac gcgtgtacgc atgtaacatt atactgaaaa
6481 ccttgcttta gaaggttttg ggacgctcga aggctttaat ttgcggccag cttgcctcgt
6541 ccccgcctgg tcacccggcc agcgacatgg aggcccagaa taccctcctt gacagtcttg
6601 acgtgcgcag ctcaggggca tgatgtgact gtcgcccgta catttagccc atacatcccc
6661 atgtataatc atttgcatcc atacattttg atggccgcac ggcgcgaagc aaaaattacg
6721 gctcctcgct gcagacctgc gagcagggaa acgctcccct cacagacgcg ttgaattgtc
6781 cccacgccgc gcccctgtag agaaatataa aaggttagga tttgccactg aggttcttct
6841 ttcatatact tccttttaaa atcttgctag gatacagttc tcacatcaca tccgaacata
6901 aacaaccgga tcctacaatt atgtctacta ctttggatga tactgcttat agatacagaa
6961 cttctgttcc aggtgatgct gaagctattg aagctttgga tggttctttc actaccgata
7021 ctgtttttag agttactgct actggtgatg gtttcacttt gagagaagtt ccagttgatc
7081 caccattgac taaggttttc ccagatgatg aatccgatga tgaatccgat gctggtgaag
7141 atggtgatcc agattctaga actttcgttg cttatggtga tgatggtgat ttggctggtt
7201 tcgttgttgt ttcttattct ggttggaaca gaagattgac tgttgaagat attgaagttg
7261 ctccagaaca tagaggtcat ggtgttggta gagctttgat gggtttggct actgaattcg
7321 ccagagaaag aggtgctggt catttgtggt tggaagttac caatgttaat cggtgtttaa
7381 accccagcgc ctggcgggcg ctcacaattc cacacaacat acgagccgga agcataaagt
7441 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
7501 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
7561 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
7621 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
7681 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
7741 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
7801 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
7861 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
7921 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
7981 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
8041 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
8101 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
8161 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
8221 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
8281 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
8341 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
8401 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
8461 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
8521 ctgacagtta ccaatgcttg atcagtgagg cacctatctc agcgatctgt ctatttcgtt
8581 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
8641 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
8701 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
8761 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
8821 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
8881 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
8941 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
9001 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
9061 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
9121 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
9181 aagtgctcat cattggaaaa cgttc
//
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