pMM114

LOCUS       pMM114               15582 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA
            targeting PDS.
ACCESSION   .
VERSION     .
KEYWORDS    pMM114
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15582)
  AUTHORS   Maher MF, Nasti RA, Vollbrecht M, Starker CG, Clark MD, Voytas DF
  TITLE     Plant gene editing through de novo induction of meristems.
  JOURNAL   Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0337-2. doi: 
            10.1038/s41587-019-0337-2.
  PUBMED    31844292
REFERENCE   2  (bases 1 to 15582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15582
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             952..2604
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(1018..1036)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     misc_RNA        3350..3425
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        3528..3707
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        3560..3743
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        5999..6344
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     6287..6307
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     misc_feature    9303..9327
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10628..11257
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11686..12759
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12825..13019
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13255..13277)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13363..13503
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13418..13437
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13518..13535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13689..14277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13769..13788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14364..15158)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtagagct caaagtttaa cgcgttagca gaaggcatgt tgttgtgact
      121 ccgaggggtt gcctcaaact ctatcttata accggcgtgg aggcatggag gcaggggtat
      181 tttggtcatt ttaatagata gtggaaaatg acgtggaatt tacttaaaga cgaagtcttt
      241 gcgacaaggg ggggcccacg ccgaatttaa tattaccggc gtggcccccc cttatcgcga
      301 gtgctttagc acgagcggtc cagatttaaa gtagaaaatt tcccgcccac tagggttaaa
      361 ggtgttcaca ctataaaagc atatacgatg tgatggtatt tgatggagcg tatattgtat
      421 caggtatttc cgttggatac gaattattcg tacgaccctc ggtaccgatc ggcgcgcctg
      481 gcagacatac tgtcccacaa atgaagatgg aatctgtaaa agaaaacgcg tgaaataatg
      541 cgtctgacaa aggttaggtc ggctgccttt aatcaatacc aaagtggtcc ctaccacgat
      601 ggaaaaactg tgcagtcggt ttggcttttt ctgacgaaca aataagattc gtggccgaca
      661 ggtgggggtc caccatgtga aggcatcttc agactccaat aatggagcaa tgacgtaagg
      721 gcttacgaaa taagtaaggg tagtttggga aatgtccact cacccgtcag tctataaata
      781 cttagcccct ccctcattgt taagggagca aaatctcaga gagatagtcc tagagagaga
      841 aagagagcaa gtagcctaga agtagtcaag gcggcgaagt attcaggcac gtggccagga
      901 agaagaaaag ccaagacgac gaaaacaggt aagagctaag cttcctgcac catggaagac
      961 gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg aaccgctgga
     1021 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     1081 gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc cgttcggttg
     1141 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     1201 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     1261 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct
     1321 accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc
     1381 ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     1441 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtgcca
     1501 gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt
     1561 ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga
     1621 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     1681 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     1741 ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa
     1801 agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa
     1861 tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc
     1921 ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact
     1981 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     2041 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     2101 gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac
     2161 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     2221 gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag
     2281 tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac
     2341 atcttcgacg ctggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     2401 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     2461 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     2521 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     2581 aagggcggaa agatcgccgt gtaactcgag atatgaagat gaagatgaaa tatttggtgt
     2641 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     2701 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat
     2761 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
     2821 tgtgctatgg tatggactat ggaatatgat taaagataag gagctccggt gacggaccca
     2881 tggcttcgtt gaacaacgga aactcgactt gccttccgca caatacatca tttcttctta
     2941 gctttttttc ttcttcttcg ttcatacagt ttttttttgt ttatcagctt acattttctt
     3001 gaaccgtagc tttcgttttc ttctttttaa ctttccattc ggagtttttg tatcttgttt
     3061 catagtttgt cccaggatta gaatgattag gcatcgaacc ttcaagaatt tgattgaata
     3121 aaacatcttc attcttaaga tatgaagata atcttcaaaa ggcccctggg aatctgaaag
     3181 aagagaagca ggcccattta tatgggaaag aacaatagta tttcttatat aggcccattt
     3241 aagttgaaaa caatcttcaa aagtcccaca tcgcttagat aagaaaacga agctgagttt
     3301 atatacagct agagtcgaag tagtgattgt tggtagtagc gactccatgg ttttagagct
     3361 agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc
     3421 ggtgcttttt ttcccgggcg cgccgatcat gagcggagaa ttaagggagt cacgttatga
     3481 cccccgccga tgacgcggga caagccgttt tacgtttgga actgacagaa ccgcaacgtt
     3541 gaaggagcca ctcagccgcg ggtttctgga gtttaatgag ctaagcacat acgtcagaaa
     3601 ccattattgc gcgttcaaaa gtcgcctaag gtcactatca gctagcaaat atttcttgtc
     3661 aaaaatgctc cactgacgtt ccataaattc ccctcggtat ccaattagag tctcatattc
     3721 actctcaatc caaataatct gcaccgtacc tgcagggtcc gagctaggtc acagaagcgc
     3781 tcaggaaggc cgctgagata gaggcatggc ggccaatgcg ggcggcggtg gagcgggagg
     3841 aggcagcggc agcggcagcg tggctgcgcc ggcggtgtgc cgccccagcg gctcgcggtg
     3901 gacgccgacg ccggagcaga tcaggatgct gaaggagctg tactacggct gcggcatccg
     3961 gtcgcccagc tcggagcaga tccagcgcat caccgccatg ctgcggcagc acggcaagat
     4021 cgagggcaag aacgtcttct actggttcca gaaccacaag gcccgcgagc gccagaagcg
     4081 ccgcctcacc agcctcgacg tgaacgtgcc cgccgccggc gcggccgacg ccaccaccag
     4141 ccaactcggc gtcctctcgc tgtcgtcgcc gccgccttca ggcgcggcgc ctccctcgcc
     4201 caccctcggc ttctacgccg ccggcaatgg cggcggatcg gctgtgctgc tggacacgag
     4261 ttccgactgg ggcagcagcg gcgctgcgat ggccaccgag acatgcttcc tccaggacta
     4321 catgggcgtg acggacacgg gcagctcgtc gcagtggcca cgcttctcgt cgtcggacac
     4381 gataatggcg gcggccgcgg cgcgggcggc gacgacgcgg gcgcccgaga ctctccctct
     4441 cttcccgacc tgcggcgacg acggcggcag cggtagcagc agctacttgc cgttctgggg
     4501 tgccgcgtcc acaactgccg gcgccacttc ttccgttgcg atccagcagc aacaccagct
     4561 gcaggagcag tacagctttt acagcaacag caacagcacc cagctggccg gcaccggcaa
     4621 ccaagacgta tcggcaacag cagcagcagc cgccgccctg gagctgagcc tcagctcatg
     4681 gtgctcccct taccctgctg cagggagtat gtgagagcaa cgcgagctgc cactgctctt
     4741 cacttatgtc tctggaatgg aaggaggagg aagtgagcat agcgttggtg cgttgctgtc
     4801 attgtcctag gttagtagct agtgccagtt actagtaagc atcaggcata ggagtatgta
     4861 gtagaagcat gcacgttgcc ggccagccag gctttagacg ggaaaagaat ttggtgcagc
     4921 cggctgcaaa acaggatgtt tacagccccc ccctcgagcc ctagacttgt ccatcttctg
     4981 gattggccaa gttaattaat gtatgaaata aaaggatgca cacatagtga catgctaatc
     5041 actataatgt gggcatcaaa gttgtgtgtt atgtgtaatt actaattatc tgaataagag
     5101 aaagagatca tccatatttc ttatcctaaa tgaatgtcac gtgtctttat aattctttga
     5161 tgaaccagat gcattttatt aaccaattcc atatacatat aaatattaat catatataat
     5221 taatatcaat tgggttagca aaacaaatct agtctaggtg tgttttgcta attattgggg
     5281 gatagtgcaa aaagaaatct acgttctcaa taattcagat agaaaactta ataaagtgag
     5341 ataatttaca tagattgctt ttatcctttg atatatgtga aaccatgcat gatataagga
     5401 aaatagatag agaaataatt ttttacatcg ttgaatatgt aaacaattta attcaagaag
     5461 ctaggaatat aaatattgag gagtttatga ttagagctct cccggcgcgc cagatttgcc
     5521 ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac gcagcaggta
     5581 tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt cccaagaagg
     5641 ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag aaagatatat
     5701 ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt cacaaaccaa
     5761 ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca aaggccatgg
     5821 agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc gaacagttca
     5881 tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg gtggagcacg
     5941 acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa agggcaattg
     6001 agacttttca acaaagggta atatccggaa acctcctcgg attccattgc ccagctatct
     6061 gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc catcattgcg
     6121 ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa gatggacccc
     6181 cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca aagcaagtgg
     6241 attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat ccttcgcaag
     6301 acccttcctc tatataagga agttcatttc atttggagag aacacggggg actcctgcag
     6361 gatggatctg cgtctaattt tcggtccaac ttgcacagga aagacgtcga ccgcgatacg
     6421 tcttgcccag cagactggcc ttccagtcct ttcgctcgat cgggtccaat gctgtcctca
     6481 actgtcaacc ggaagcggac gaccaacagt ggaagaactg aaaggaacga cccgtctata
     6541 ccttgaagat cggcctctgg tgaagggtat catcgcagcc aagcaagctc acgaaaggct
     6601 gatcggggaa gtgtacaatt atgaggccca cggcgggctt attcttgagg gaggatctat
     6661 ctcgttgctc aggtgcatgg cgcaaagcag ttattggagt accgattttc gttggcatat
     6721 tattcgccac aagttagcag acgaggagac attcatgaac gcggccaagg ccagagttag
     6781 gcagatgttg cgccctgctg taggcccatc tattattcaa gagttggttc atctttggaa
     6841 tgagcctcgg ctgaggccca tactgaaaga gatcgacgga tatcgatatg ccatgttatt
     6901 tgctagccag aaccagatca cacccgatat gctattgcag cttgacccag atatggaggg
     6961 tgagttgatt catggaatcg ctcaggagta tctcatccat gcgcgccggc aggagcagga
     7021 attccctcca gtgagcgtgg tcgctttcga aggattcgaa ggtccaccgt tcggaatgtg
     7081 ctagctcgag ccctagactt gtccatcttc tggattggcc aagttaatta atgtatgaaa
     7141 taaaaggatg cacacatagt gacatgctaa tcactataat gtgggcatca aagttgtgtg
     7201 ttatgtgtaa ttactaatta tctgaataag agaaagagat catccatatt tcttatccta
     7261 aatgaatgtc acgtgtcttt ataattcttt gatgaaccag atgcatttta ttaaccaatt
     7321 ccatatacat ataaatatta atcatatata attaatatca attgggttag caaaacaaat
     7381 ctagtctagg tgtgttttgc taattattgg gggatagtgc aaaaagaaat ctacgttctc
     7441 aataattcag atagaaaact taataaagtg agataattta catagattgc ttttatcctt
     7501 tgatatatgt gaaaccatgc atgatataag gaaaatagat agagaaataa ttttttacat
     7561 cgttgaatat gtaaacaatt taattcaaga agctaggaat ataaatattg aggagtttat
     7621 gattagagct cagtgtttga tcgccggcgg taccgagtgt acttcaagtc agtgggaaat
     7681 caataaaatg attattttat gaatatattt cattgtgcaa gtagatagaa attacatatg
     7741 ttacataaca cacgaaataa acaaaaaaag acaatccaaa aacaaacacc ccaaaaaaaa
     7801 taatcacttt agataaactc gtatgaggag aggcacgttc agtgactcga cgattcccga
     7861 gcaaaaaaag tctccccgtc acacatgtag tgggtgacgc aattatcttt aaagtaatcc
     7921 ttctgttgac ttgtcattga taacatccag tcttcgtcag gattgcaaag aattatagaa
     7981 gggatcccac cttttatttt cttctttttt ccatatttag ggttgacagt gaaatcagac
     8041 tggcaaccta ttaattgctt ccacaatggg acgaacttga aggggatgtc gtcgatgata
     8101 ttataggtgg cgtgttcatc gtagttggtg aaatcgatgg taccgttcca atagttgtgt
     8161 cgtccgagac ttctagccca ggtggtcttt ccggtacgag ttggtccgca gatgtagagg
     8221 ctggggtgtc ggattccatt ccttccattg tccttgttaa atcggccatc cattcaaggt
     8281 cagattgagc ttgttggtat gagacaggat gtatgtaagt ataagcgtct atgcttacat
     8341 ggtatagatg ggtttccctc caggagtgta gatcttcgtg gcagcgaaga tctgattctg
     8401 tgaagggcga cacatacggt tcaggttgtg gagggaataa tttgttggct gaatattcca
     8461 gccattgaag ctttgttgcc cattcatgag ggaattcttc cttgatcatg tcaagatatt
     8521 cctccttaga cgttgcagtc tggataatag ttctccatcg tgcgtcagat ttgcgaggag
     8581 aaaccttatg atctcggaaa tctcctctgg ttttaatatc tccgtccttt gatatgtaat
     8641 caaggacttg tttagagttt ctagctggct ggatattagg gtgatttcct tcaaaatcga
     8701 aaaaagaagg atccctaata caaggttttt tatcaagctg gagaagagca tgatagtggg
     8761 tagtgccatc ttgatgaagc tcagaagcaa caccaaggaa gaaaataaga aaaggtgtga
     8821 gtttctccca gagaaactgg aataaatcat ctctttgaga tgagcacttg ggataggtaa
     8881 ggaaaacata tttagattgg agtctgaagt tcttactagc agaaggcatg ttgttgtgac
     8941 tccgaggggt tgcctcaaac tctatcttat aaccggcgtg gaggcatgga ggcaggggta
     9001 ttttggtcat tttaatagat agtggaaaat gacgtggaat ttacttaaag acgaagtctt
     9061 tgcgacaagg gggggcccac gccgaattta atattaccgg cgtggccccc ccttatcgcg
     9121 agtgctttag cacgagcggt ccagatttaa agtagaaaat ttcccgccca ctagggttaa
     9181 aggtgttcac actataaaag catatacgat gtgatggtat ttgatggagc gtatattgta
     9241 tcaggtattt ccgttggata cgaattattc gtacgaccct catagtttaa actatcagtg
     9301 tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta gaataatcgg
     9361 atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc atgccaacca
     9421 cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta tagtgcagtc
     9481 ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa gtcctaagtt
     9541 acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg ttttagtcgc
     9601 ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa gagcgccgcc
     9661 gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac caaccaacgg
     9721 gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac cggcaccagg
     9781 cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga cgttgtgaca
     9841 gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat tgccgagcgc
     9901 atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga caccaccacg
     9961 ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga gcgttcccta
    10021 atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt gaagtttggc
    10081 ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat cgaccaggaa
    10141 ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc
    10201 gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg tgccttccgt
    10261 gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg ccaagaggaa
    10321 caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc gaagagatcg
    10381 aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacggc tcaaccgtgc
    10441 ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg ccggccagct
    10501 tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca
    10561 gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa atacgcaagg
    10621 ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca agacgaccat
    10681 cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt tagtcgattc
    10741 cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt
    10801 tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt
    10861 cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga tcaaggcagc
    10921 cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca ccgccgacct
    10981 ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag cggcctttgt
    11041 cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg cgctggccgg
    11101 gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc caggcactgc
    11161 cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc gcgaggtcca
    11221 ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa agagaaaatg
    11281 agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag caaggctgca
    11341 acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca gttgccggcg
    11401 gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat taccgagctg
    11461 ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa tgagtagatg
    11521 aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca ccgacgccgt
    11581 ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct gggttgcctg
    11641 ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa
    11701 ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga gaagttgaag
    11761 gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg
    11821 caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg
    11881 tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc gatgctctat
    11941 gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag
    12001 cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca cgtagaggtt
    12061 tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact gatggcggtt
    12121 tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa gcccggccgc
    12181 gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga tggcggaaag
    12241 cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt tgccatgcag
    12301 cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga agccttgatt
    12361 agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga gatcgagcta
    12421 gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct gacggttcac
    12481 cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct ggcacgccgc
    12541 gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg cagtggcagc
    12601 gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg
    12661 ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt catgcgctac
    12721 cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca gatgctaggg
    12781 caaattgccc tagcagggga aaaaggtcga aaaggcctct ttcctgtgga tagcacgtac
    12841 attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa cccaaagccg
    12901 tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa aggcgatttt
    12961 tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc ctgtgcataa
    13021 ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc
    13081 tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc aaaaatggct
    13141 ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc actcgaccgc
    13201 cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
    13261 acacatgcag ctcccggaaa cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
    13321 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
    13381 acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg
    13441 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    13501 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    13561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    13621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    13681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    13741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    13801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    13861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    13921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    13981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    14041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    14101 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
    14161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    14221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    14281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    14341 attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt
    14401 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
    14461 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
    14521 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
    14581 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
    14641 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
    14701 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
    14761 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
    14821 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
    14881 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
    14941 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
    15001 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
    15061 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
    15121 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
    15181 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
    15241 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
    15301 tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa
    15361 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
    15421 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
    15481 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
    15541 ttttgtgccg agctgccggt cggggagctg ttggctggct gg
//