pAB744

LOCUS       pAB744            12889 bp ds-DNA     circular SYN 16-NOV-2021
DEFINITION  Expressing the whole n-butanol pathway..
ACCESSION   .
VERSION     .
KEYWORDS    pAB744
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12889)
  TITLE     n-butanol pathway plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 16, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12889
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     807..826
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(893..995)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1112..1130)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     rep_origin      2388..3159
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             3160..3822
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /label=pBBR1 Rep
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     4553..4572
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     terminator      4696..4743
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     4739..4757
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(4810..4833)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     RBS             11175..11197
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    11212..11236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(11236..11255)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11237..11255)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(11300..12094)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11411..11430)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     12021..12040
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
       61 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtaa gttagctcac
      121 tcattaggca ccgggatctc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
      181 ccggtgggcg cggggcatga ctataatgaa tcggccaacg cgcggggaga ggcggtttgc
      241 gtattgggcg catgcataaa aactgttgta attcattaag cattctgccg acatggaagc
      301 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
      361 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
      421 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
      481 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
      541 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
      601 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
      661 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
      721 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
      781 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
      841 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
      901 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
      961 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
     1021 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
     1081 ccgtcacagg tatttattcg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     1141 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     1201 gcccgcgttc ctgctggcgc tgggcctgtt tctggcgctg gacttcccgc tgttccgtca
     1261 gcagcttttc gcccacggcc ttgatgatcg cggcggcctt ggcctgcata tcccgattca
     1321 acggccccag ggcgtccaga acgggcttca ggcgctcccg aaggtctcgg gccgtctctt
     1381 gggcttgatc ggccttcttg cgcatctcac gcgctcctgc ggcggcctgt agggcaggct
     1441 catacccctg ccgaaccgct tttgtcagcc ggtcggccac ggcttccggc gtctcaacgc
     1501 gctttgagat tcccagcttt tcggccaatc cctgcggtgc ataggcgcgt ggctcgaccg
     1561 cttgcgggct gatggtgacg tggcccactg gtggccgctc cagggcctcg tagaacgcct
     1621 gaatgcgcgt gtgacgtgcc ttgctgccct cgatgccccg ttgcagccct agatcggcca
     1681 cagcggccgc aaacgtggtc tggtcgcggg tcatctgcgc tttgttgccg atgaactcct
     1741 tggccgacag cctgccgtcc tgcgtcagcg gcaccacgaa cgcggtcatg tgcgggctgg
     1801 tttcgtcacg gtggatgctg gccgtcacga tgcgatccgc cccgtacttg tccgccagcc
     1861 acttgtgcgc cttctcgaag aacgccgcct gctgttcttg gctggccgac ttccaccatt
     1921 ccgggctggc cgtcatgacg tactcgaccg ccaacacagc gtccttgcgc cgcttctctg
     1981 gcagcaactc gcgcagtcgg cccatcgctt catcggtgct gctggccgcc cagtgctcgt
     2041 tctctggcgt cctgctggcg tcagcgttgg gcgtctcgcg ctcgcggtag gcgtgcttga
     2101 gactggccgc cacgttgccc attttcgcca gcttcttgca tcgcatgatc gcgtatgccg
     2161 ccatgcctgc ccctcccttt tggtgtccaa ccggctcgac gggggcagcg caaggcggtg
     2221 cctccggcgg gccactcaat gcttgagtat actcactaga ctttgcttcg caaagtcgtg
     2281 accgcctacg gcggctgcgg cgccctacgg gcttgctctc cgggcttcgc cctgcgcggt
     2341 cgctgcgctc ccttgccagc ccgtggatat gtggacgatg gccgcgagcg gccaccggct
     2401 ggctcgcttc gctcggcccg tggacaaccc tgctggacaa gctgatggac aggctgcgcc
     2461 tgcccacgag cttgaccaca gggattgccc accggctacc cagccttcga ccacataccc
     2521 accggctcca actgcgcggc ctgcggcctt gccccatcaa tttttttaat tttctctggg
     2581 gaaaagcctc cggcctgcgg cctgcgcgct tcgcttgccg gttggacacc aagtggaagg
     2641 cgggtcaagg ctcgcgcagc gaccgcgcag cggcttggcc ttgacgcgcc tggaacgacc
     2701 caagcctatg cgagtggggg cagtcgaagg gcgaagcccg cccgcctgcc ccccgagcct
     2761 cacggcggcg agtgcggggg ttccaagggg gcagcgccac cttgggcaag gccgaaggcc
     2821 gcgcagtcga tcaacaagcc ccggaggggc cactttttgc cggaggggga gccgcgccga
     2881 aggcgtgggg gaaccccgca ggggtgccct tctttgggca ccaaagaact agatataggg
     2941 cgaaatgcga aagacttaaa aatcaacaac ttaaaaaagg ggggtacgca acagctcatt
     3001 gcggcacccc ccgcaatagc tcattgcgta ggttaaagaa aatctgtaat tgactgccac
     3061 ttttacgcaa cgcataattg ttgtcgcgct gccgaaaagt tgcagctgat tgcgcatggt
     3121 gccgcaaccg tgcggcaccc ctaccgcatg gagataagca tggccacgca gtccagagaa
     3181 atcggcattc aagccaagaa caagcccggt cactgggtgc aaacggaacg caaagcgcat
     3241 gaggcgtggg ccgggcttat tgcgaggaaa cccacggcgg caatgctgct gcatcacctc
     3301 gtggcgcaga tgggccacca gaacgccgtg gtggtcagcc agaagacact ttccaagctc
     3361 atcggacgtt ctttgcggac ggtccaatac gcagtcaagg acttggtggc cgagcgctgg
     3421 atctccgtcg tgaagctcaa cggccccggc accgtgtcgg cctacgtggt caatgaccgc
     3481 gtggcgtggg gccagccccg cgaccagttg cgcctgtcgg tgttcagtgc cgccgtggtg
     3541 gttgatcacg acgaccagga cgaatcgctg ttggggcatg gcgacctgcg ccgcatcccg
     3601 accctgtatc cgggcgagca gcaactaccg accggccccg gcgaggagcc gcccagccag
     3661 cccggcattc cgggcatgga accagacctg ccagccttga ccgaaacgga ggaatgggaa
     3721 cggcgcgggc agcagcgcct gccgatgccc gatgagccgt gttttctgga cgatggcgag
     3781 ccgttggagc cgccgacacg ggtcacgctg ccgcgccggt agcacttggg ttgcgcagca
     3841 acccgtaagt gcgctgttcc agactatcgg ctgtagccgc ctcgccgccc tataccttgt
     3901 ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc
     3961 cggcggcacc tcgctaacgg attcaccgtt tttatcaggc tctgggaggc agaataaatg
     4021 atcatatcgt caattattac ctccacgggg agagcctgag caaactggcc tcaggcattt
     4081 gagaagcaca cggtcacact gcttccggta gtcaataaac cggtaaacca gcaatagaca
     4141 taagcggcta tttaacgacc ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat
     4201 ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc gtttaagggc
     4261 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tacggcctat
     4321 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     4381 cttacaattt ccattcgcca ttcaggctgc gcaactgttg ggaagggcgt gaaccatcac
     4441 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     4501 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     4561 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     4621 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca atccggatat
     4681 agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc
     4741 tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt tgttagcagc
     4801 cggatctcac ttttcgaact gcggatgcga ccactgcagt caaaccatgt attggccgcc
     4861 gttgatggtg agcgtcgaac cggtgatgta gcccgcatcg tcgccggcga ggaacaccac
     4921 ggcgcgcgcg atctcctcgg cttcgccgag ccggttgcac ggaatcagcg gcaggatcgc
     4981 cttgtcgagc acgtccttcg gcacggcctg caccatttcg gtgttgatgt agcccgggca
     5041 gatcacattg acggtgatgc cgccgcgcgc attctccagc gccagcgctt tggtgaagcc
     5101 gatgtcgccc gccttcgcgg ccgaatagtt cacctgaccg aactgcccct tctgaccgtt
     5161 gatcgacgag atgttgacga tgcgcccgaa cttgcgcgtg cgcatgcctt cgatcacctg
     5221 ccgggtcatg ttgaacagcg agccgagatt ggtgttgatc accgcattcc attgctcgag
     5281 cgtcatcttg tggaaggcgc cgtcgcgcgt gatgccggca ttgttgacca gaacatcgac
     5341 gggaccgagt tcggcctcga ctttcttgac gccctcggca caggcatcga acgaactcac
     5401 gtcccattta tataccggaa tacccgtctc gtttttgaat ttctccgctg ccgaatcgtt
     5461 gcccgcataa gacgctgcga ctttgtagcc cgccgctttc aacgccttgc tgattgctgc
     5521 accgataccg cgcgtccccc cagtcaccaa cgccacccgc gtcattgcaa agctcctccc
     5581 gcaattcctt ttttgtctag aacgcgattg tgtcttttcg aataaaagga tgctcgctcg
     5641 tgaacatcaa gcaaaaacgc ccggcaatct gccgggcgtt tgaacaatcg ttcttgcact
     5701 gcaactgatc agtccgtgta tcgcgacgtc gtcgcgacac acggcgatca atcgcgcgcg
     5761 aggcacatcg cgatgcccat gccaccgccg atgcaaagcg tggcaagacc tttcttcgaa
     5821 tcgcgcttct gcatctcgtg cagcagcgtc acaaggacgc gcgcccccga cgcaccgatc
     5881 ggatgaccaa tcgcgatcgc accgccattg acgttaacct tagaagtatc ccagccgaga
     5941 tccttgttca ccgcacaggc ctgcgccgcg aaggcttcgt tggcttcgat cagatcgaga
     6001 tcgccgaccg accagccagc cttcttcagc gccgcacgcg acgccgggat cggccctgag
     6061 cccatgatct tcggatcgac gccggcctgg ccccacgaca cgatgcgggc cagcggcttc
     6121 ttgccgagct tctccgcctg cttggcggtc atcagcacca ccgcagcggc gccgtcgttg
     6181 attcccgacg cagaacccgc cgtgacggtg ccgtccttct cgaacgccgg cttcagcttg
     6241 gccatcgctt cgagcgtggc gccgtggcgc ggatactcgt cggtgtcgac cacgatgtcg
     6301 cccttgcggg tcttgatcgt caccgggacg atctcgtcct tgaacttgcc ggccttctgc
     6361 gcagcttcgg ccttctgctg cgaagccacc gcgaattcat cctgctgatc gcgggtgatc
     6421 tgccactgcc gcgcgacgtt ctcggcggtg ttgcccatgt ggtaaccgtt gaaggcatcc
     6481 cacaggccgt ccttgagcat ggtgtcgacg agttcgaccg cgcccatctt tacgccgccg
     6541 cgcaaatatt gcgcatgcgg cgccatgctc atcgattcct ggccgcccgc gatgacgata
     6601 tcggagtcgc cgttcatcag tgcctggtaa ccgagcgcga ccgagcgaag accggagccg
     6661 cagagctggt tgatgcccca tgccgggctc tcgaccggaa taccggcggc aatcgaggcc
     6721 tgcctcgccg gattctgccc ctgaccggcg gtgaggatct ggcccatgat cacttcggag
     6781 acctgcgccg gatcgatgcc gccgcgctcc agcgcagctt tgatcgcgac cgcgccgagc
     6841 tcgtgcgccg gcgtattggc gaaggcgccg ttaaaacttc cgacagcggt gcgtgcggcg
     6901 ctgacgatga cgacatcgtc cgacatggac atctcctggt tagaagcttt tgatgtagat
     6961 gtccttcagc tccgagatca gcgggtagcg cgggttcgcc gtggtgcact ggtcatcgaa
     7021 cgccagctcg ctcatcttgt cgagggtgtt gtagaagtcc ttcttattga tgcccgcggc
     7081 ggagatattc tgcgggatcg acagatcgat cttgagcttg ctgatcgctt cgatcagcgc
     7141 ggtcaccttt tcggtgtccg aggtgccctt cagattcagg tattcggcga tttccgcgta
     7201 cttgcgcttc gcattcggcg acttgtactg cgggaacgcg gtctgcttcg tcgggcagtc
     7261 ggtcgcgtta tacttgatga cctcctcgat gagcaccgcg caggcgatgc cgtgcggcac
     7321 gtggtgcatg gcgccgagct tatgcgccat gctgtggcag acgcccagga aggcgttggc
     7381 gaaggccatg ccggcgatgt tgctggcgtg ggccatcttt tcgcgcgctt cgatgtcgtt
     7441 ggtgccgttt ttgtacgcgc gcggcaggta tttgaagatc atcttgatcg cgcgcagcgc
     7501 gagttcgtcg gtatagtcgg tggccatcac cgacacatac gcctcgatcg cgtggaccag
     7561 cgcgtcgatg ccggtggcgg cggtgagctt ccgcggcatg ttcagcatca gctcggtgtc
     7621 gatgatcgcc atattcgggg tcagctcata gctggtcagc gggtacttca tccccgtctc
     7681 gtcgttggtg atgaccgcga acggggtggc ctccgacccc gtgccggcgg tggtcgggat
     7741 cgcgacggag atcgccttgg tgcccagctt cgggaaattg cagatgcgct tgcggatgtc
     7801 catgaagttg atggccagat tctcgatctc cgcctccgga tactcgtaca gcaggtgcat
     7861 caccttggcc gcatccatcg ggctgccgcc gccgatcgag atgatcgtgt ccggctcgaa
     7921 gttcagcatc tccttcgcgc ctttcttcac cgaatcgatg gtcgggtcgg atttgatgtc
     7981 ggtgaagatg ctgtacttga tgtcgatttc gtcgaggacc ttggtgatct tgttgacgta
     8041 gcccagcttg aacaggtcct tgtcggtcac gatgaaggcc cgtttcttgt tcatgtcctt
     8101 gagctccttc agggcgaagc ggaggcagcc gtacttgaag tagatcttct gcggcacctt
     8161 gaaccacagc atgttttccc ggcgttcggc gacggatttg atgttcagca ggtgttttgg
     8221 ttccacgttc tgcgagacgc tattgccgcc ccaggtgccg cacccgagcg tgaagctcgg
     8281 cgcgatggcg aaattgtaga ggtcgcccga ggcgccctgc gagcttggca tgttgatgaa
     8341 ggtgcgggag gtcttcatgg cgaggccgaa ctctttcact ttatccttat tgttctggct
     8401 gtcgatatac agcgacgagg tatggcccga gccgcccagc tcgatcaggc gctgggcctt
     8461 cttcagggct tcgtcgaagt ctttgacctt atacatcgcc agcaccgggc tcagcttctc
     8521 gtggctgaac agctccgact tctcgacgga ctgcacctcg ccgatgagga tcttggtggt
     8581 ctgcggcacc tcgatgccgg ccatcttggc gatgatatag gccgacttgc ccacgatgtc
     8641 cgcattgatc gcgccgttct taaacatggt ctccttgatc ttcgcgatct cattctggtt
     8701 caggatatac gagccgcgct tgacaaactc ctccttgact ttctcgtaga tgctgttcat
     8761 gaccaggatg ctctgctcgc tggcgcagat caccccgttg tcgtaggtct tggacaggat
     8821 gatgctcgac accgccatgt cgatgtcggc gctctcgtcg atgatggccg gggtgttccc
     8881 cgcgcccacg ccgatcgcgg gcttgccgga cgagtacgcc gctttgacca tcgaggggcc
     8941 gccggtggcg aggatgatgt cggcctccga catgagatcc tggctcagct cgatcgacgg
     9001 ttcatcgatc cagccgatga tgttcttcgg cgcgccggcc ttcacggcgg cgtccaggat
     9061 gagcttggcg gccgcgatgg tggacttctt ggcgcgcgga tgcgggctga agaagatcgc
     9121 gttgcgggtc ttcagcgaga tcaggctctt gaagatggcc gtggaggtcg ggttggtggt
     9181 ggggacgatc gccgcgacga tcccgatggg ttccgccacc ttggtgatgc cgaggctgtc
     9241 gtcgtggtcg atgatgccgc aggtcttctc gttcttgtac ttattgtaga tgtactccgc
     9301 ggcgaagtgg ttcttgatga tcttatcctc caccaggccg atgccggtct cctcgacggc
     9361 cagcttggcg aggttgatgc gctccttggc ggcggcgatg gcgcactgct tgaagatttt
     9421 gtcgacctgc tcctgcgtgt aggtggcgaa cttcttctgg gcctcccgca gctcattcag
     9481 cttctgcttc agctcctttt ggttggtgac cttcatgcga aacgatcctc atcctgaatt
     9541 cttagatgcg gtcgaagcgc tcgacctccg cctcgtagtt gatgccctcg acatcgaagc
     9601 cgttgctggc gaggaagtcg tggcggtagc cggccaggtc ggtcaggctc tcggcgttct
     9661 cgccggtgac cttctccatc agcgcgctga ccgccttttg gacgtcctcc tcgagctccc
     9721 aatcgtcgat ccggatccga ttttcctcgt ccaccgggat ggtcccgtcc ttgcggtaca
     9781 ggcgctcggc gtacaggcgc gtgatctgct cgatgcagcc ttcatggttg cccttctctt
     9841 tcatcacctt aaacagggac gcgaggtaca gcgggatgac cgggatcacg gcgctcgccc
     9901 gggtgaccag gcccttgttg accgagacga acgcgcggat ggaggggttc tccttattga
     9961 ggcggtgcgc ggtggcctcg aggtgctcct tggccttgcc gatggtgccc ttccggtaga
    10021 gggcctgggt cgcttccggg ccgatatacg agtaggcgag ggtgatgcag ccctcctcca
    10081 gcaggccctc cttgctcagc tgcttgatcc agcgttccca gtcctcgccg cccatcacct
    10141 tgacggtggc cgccgcctcc tcatcgttgg ccggttccgc gctgatctcc ttgagctccc
    10201 cggtgaacgg gtcgacggtc ttgcccgtga aggtcttgcc gaacggcttc aggacgcttt
    10261 tgtgcatgat cccggtatcc gggtccgtgc gcaccgggct cgccagcgag tagacgatca
    10321 ggtcaaactt gatgcccttc tttttggcct cctcgatgac ctgcgccttg atctcatccg
    10381 agaacgcgtc gccatcgatg gtcacgctgt acaggccctc gcgcttggcc gcctcgtcaa
    10441 aggccagatt gttgtaccag cccggggtgc cgtacttcgt ctcgctgccg gccttctcga
    10501 acgagacgcc gatcgtggcg gcgccgtagc cgaaggcggc ggtgatgcgc gacgccaggc
    10561 catagccgtt cgagcagccg aggacgagga cgttcttcgg ggccttcgcg cccgccttga
    10621 cttccgcggt gatgcgcttc ttggtgtact cgatctggtc ctcgacgccc tttttgcagc
    10681 cctgcgggtg ggcgttcagg cagatattgt tgcgcaccat cggcttgacg atcatgcgaa
    10741 acgatcctca tcctgcatgc atttacggca gcttcaccac cgcttccccg gtcacggcca
    10801 gcgcgccgcc ctgcgtaaag atgcgggtgg tgagggtggc gattggcttg tcctcgcgca
    10861 gcgcggtgac ttccacctcg gccgtgactt cgtcgccgac gaacaccggc agcttaaagg
    10921 acaggctctg gcccaggtag atggagcctt tccccggcag ctgttggccg aggaggccgc
    10981 tgaacaggct cgcgaggagc atgccatgca cgatcgggcg ttcgaacgcc gtggtcgccg
    11041 caaacgccgg gtccagatgc agcgggttga agtcctcgga gagggccgcg aacgcggcga
    11101 cttccgccgc gccaaaccgc ttgctcaggc gcgctttctg gcccacctcc agggactgcg
    11161 cggacatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt
    11221 atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatcga gatcaattcc
    11281 gcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
    11341 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
    11401 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
    11461 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
    11521 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catccgcgcc ttgagcctgg
    11581 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
    11641 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
    11701 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
    11761 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
    11821 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
    11881 tggccagcca cgatagccgc gctgcctcgt cttggagttc attcagggca ccggacaggt
    11941 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
    12001 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
    12061 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
    12121 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
    12181 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg
    12241 ctgtccataa aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct
    12301 ttctctttgc gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg
    12361 gtcagcaccg tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc
    12421 gccctgagtg cttgcggcag cgtgaagcta gcggaattca ttaagttctg tctcggcgcg
    12481 tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
    12541 acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
    12601 gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
    12661 cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
    12721 taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
    12781 gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
    12841 caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcg
//