psiCHECK-2-h2.82705/9

LOCUS       psiCHECK-2-h2.82705/9               6619 bp DNA     circular SYN 09-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    2357-psiCHECK-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        62..253
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     rep_origin      270..405
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      688..697
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             694..1629
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     misc_feature    1681..2018
                     /label=-6
     polyA_signal    2034..2082
                     /note="synthetic polyadenylation signal"
     promoter        2091..2842
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     regulatory      2872..2881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2878..4530
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /db_xref="GI:42718124"
                     /protein_id="AAS38485.1"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     RBS             3837..3845
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     polyA_signal    4574..4695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4828..4932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4933..5793
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5964..6552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta gagcggccgc
     1681 cagaaatgtg aatgatgtcc ctcatgtatt gcttatttct tgagctttta ggtacaactt
     1741 ggcaaatata ccaaatgata gagaggaaaa atgaaggtat ttttacattg aaaatttttc
     1801 actggcgaag ctcttttcct gaaggaaaca tcactcatgt ttagactaca ttatgtgcac
     1861 atcgtgacct agaattggaa acccagtggc tactgaactg gggctcctct aaagccaaac
     1921 caaaccattt gagcaagaac tatggactat tcatacagtt tatcaaattt agccagatga
     1981 cagagacagt gtctgacaaa aggttgaatc tagtaaaagc ggccgctggc cgcaataaaa
     2041 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaggatcta aatgagtctt
     2101 cggacctcgc gggggccgct taagcggtgg ttagggtttg tctgacgcgg ggggaggggg
     2161 aaggaacgaa acactctcat tcggaggcgg ctcggggttt ggtcttggtg gccacgggca
     2221 cgcagaagag cgccgcgatc ctcttaagca cccccccgcc ctccgtggag gcgggggttt
     2281 ggtcggcggg tggtaactgg cgggccgctg actcgggcgg gtcgcgcgcc ccagagtgtg
     2341 accttttcgg tctgctcgca gacccccggg cggcgccgcc gcggcggcga cgggctcgct
     2401 gggtcctagg ctccatgggg accgtatacg tggacaggct ctggagcatc cgcacgactg
     2461 cggtgatatt accggagacc ttctgcggga cgagccgggt cacgcggctg acgcggagcg
     2521 tccgttgggc gacaaacacc aggacggggc acaggtacac tatcttgtca cccggaggcg
     2581 cgagggactg caggagcttc agggagtggc gcagctgctt catccccgtg gcccgttgct
     2641 cgcgtttgct ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga
     2701 cacaaacccc gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc
     2761 ggcgcggtcc caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc
     2821 gaccctgcag cgacccgctt aaaagcttgg cattccggta ctgttggtaa agccaccatg
     2881 gccgatgcta agaacattaa gaagggccct gctcccttct accctctgga ggatggcacc
     2941 gctggcgagc agctgcacaa ggccatgaag aggtatgccc tggtgcctgg caccattgcc
     3001 ttcaccgatg cccacattga ggtggacatc acctatgccg agtacttcga gatgtctgtg
     3061 cgcctggccg aggccatgaa gaggtacggc ctgaacacca accaccgcat cgtggtgtgc
     3121 tctgagaact ctctgcagtt cttcatgcca gtgctgggcg ccctgttcat cggagtggcc
     3181 gtggcccctg ctaacgacat ttacaacgag cgcgagctgc tgaacagcat gggcatttct
     3241 cagcctaccg tggtgttcgt gtctaagaag ggcctgcaga agatcctgaa cgtgcagaag
     3301 aagctgccta tcatccagaa gatcatcatc atggactcta agaccgacta ccagggcttc
     3361 cagagcatgt acacattcgt gacatctcat ctgcctcctg gcttcaacga gtacgacttc
     3421 gtgccagagt ctttcgacag ggacaaaacc attgccctga tcatgaacag ctctgggtct
     3481 accggcctgc ctaagggcgt ggccctgcct catcgcaccg cctgtgtgcg cttctctcac
     3541 gcccgcgacc ctattttcgg caaccagatc atccccgaca ccgctattct gagcgtggtg
     3601 ccattccacc acggcttcgg catgttcacc accctgggct acctgatttg cggctttcgg
     3661 gtggtgctga tgtaccgctt cgaggaggag ctgttcctgc gcagcctgca agactacaaa
     3721 attcagtctg ccctgctggt gccaaccctg ttcagcttct tcgctaagag caccctgatc
     3781 gacaagtacg acctgtctaa cctgcacgag attgcctctg gcggcgcccc actgtctaag
     3841 gaggtgggcg aagccgtggc caagcgcttt catctgccag gcatccgcca gggctacggc
     3901 ctgaccgaga caaccagcgc cattctgatt accccagagg gcgacgacaa gcctggcgcc
     3961 gtgggcaagg tggtgccatt cttcgaggcc aaggtggtgg acctggacac cggcaagacc
     4021 ctgggagtga accagcgcgg cgagctgtgt gtgcgcggcc ctatgattat gtccggctac
     4081 gtgaataacc ctgaggccac aaacgccctg atcgacaagg acggctggct gcactctggc
     4141 gacattgcct actgggacga ggacgagcac ttcttcatcg tggaccgcct gaagtctctg
     4201 atcaagtaca agggctacca ggtggcccca gccgagctgg agtctatcct gctgcagcac
     4261 cctaacattt tcgacgccgg agtggccggc ctgcccgacg acgatgccgg cgagctgcct
     4321 gccgccgtcg tcgtgctgga acacggcaag accatgaccg agaaggagat cgtggactat
     4381 gtggccagcc aggtgacaac cgccaagaag ctgcgcggcg gagtggtgtt cgtggacgag
     4441 gtgcccaagg gcctgaccgg caagctggac gcccgcaaga tccgcgagat cctgatcaag
     4501 gctaagaaag gcggcaagat cgccgtgtaa taattctaga gtcggggcgg ccggccgctt
     4561 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     4621 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     4681 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     4741 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     4801 ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     4861 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     4921 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     4981 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     5041 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     5101 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     5161 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     5221 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     5281 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     5341 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     5401 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     5461 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     5521 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     5581 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     5641 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     5701 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     5761 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     5821 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     5881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     5941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     6001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     6061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     6121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     6181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     6241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     6301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     6361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     6421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     6481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     6541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     6601 tttgctcaca tggctcgac
//