| 载体名称: | pMAX-BFP, pMAXBFP |
|---|---|
| 载体又名: |
pMAX-BFP载体，pMAX-BFP质粒，pMAX-BFP载体质粒 pMAXBFP载体，pMAXBFP质粒，pMAXBFP载体质粒 |
| 英文名称: |
pMAX-BFP, pMAX-BFP Vector, pMAX-BFP Plasmid pMAXBFP, pMAXBFP Vector, pMAXBFP Plasmid |
| 质粒类型: | 哺乳动物细胞表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | CMV |
| 载体大小: | 3.5kb |
| 5’测序引物: | -- |
| 3’测序引物: | -- |
| 载体标签: | -- |
| 载体抗性: | 卡那霉素 |
| 克隆菌株: | DH5α,TOP10 等 |
| 宿主细胞: | 常规细胞系（293、CHO等） |
| 备注: | |
| 产品目录号: | 2178012 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pMAX-BFP载体 pMAXBFP质粒基本信息
pMAX-BFP质粒图谱 pMAXBFP载体图谱和pMAX-BFP载体序列 pMAXBFP质粒序列多克隆位点信息
pMAX-BFP质粒 pMAXBFP载体简介
pMAX-BFP质粒序列 pMAXBFP载体序列
pMAX-BFP Snapgene格式序列 pMAX-BFP .gb格式序列
LOCUS pMAX-BFP 3532 bp DNA circular SYN 31-JUL-2025
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3532)
AUTHORS DotmaticsRD
TITLE Direct Submission
JOURNAL Exported Jul 31, 2025 from SnapGene 8.1.0
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3532
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 138..517
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
/note="color: #ffffff"
promoter 518..721
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
/note="color: #ffffff; direction: RIGHT"
intron 813..945
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
/note="color: #------"
regulatory 974..983
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/note="color: #a6acb3"
/regulatory_class="other"
CDS join(980..982,983..985,986..1699)
/codon_start=1
/product="blue variant of GFP"
/label=BFP
/note="mammalian codon-optimized"
/note="This feature has 3 segments:
1: 980 .. 982 / #3366ff
2: 983 .. 985 / #3366ff / 1a
3: 986 .. 1699 / #3366ff"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLSHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIK
ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
polyA_signal 1735..1816
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
/note="color: #a6acb3"
rep_origin complement(2014..2601)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
/note="color: #ffff00"
CDS complement(2682..3476)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/note="color: #ccffcc"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagg
721 tcgtttagtg aaccgtcaga tcactagtag ctttattgcg gtagtttatc acagttaaat
781 tgctaacgca gtcagtgctc gactgatcac aggtaagtat caaggttaca agacaggttt
841 aaggaggcca atagaaactg ggcttgtcga gacagagaag attcttgcgt ttctgatagg
901 cacctattgg tcttactgac atccactttg cctttctctc cacaggggta ccgaagccgc
961 tagcgctacc ggtgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
1021 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
1081 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
1141 gcccgtgccc tggcccaccc tcgtgaccac cctgagccac ggcgtccagt gcttcagccg
1201 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
1261 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
1321 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
1381 cggcaacatc ctggggcaca agctggagta caacttcaac agccacaacg tctatatcat
1441 ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca acatcgagga
1501 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
1561 gctgctgccc gacagccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
1621 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
1681 ggacgagctg tacaagtaag agctcgatga gtttggacaa accacaacta gaatgcagtg
1741 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
1801 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
1861 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtactt aagaggggga
1921 gaccaaaggg cgagacgtta aggcctcacg tgacatgtga gcaaaaggcc agcaaaaggc
1981 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
2041 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
2101 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
2161 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
2221 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
2281 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
2341 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
2401 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
2461 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
2521 atccggcaaa caaaccacgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
2581 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
2641 tggaacgaaa actcacgtta agggattttg gtcatgccgt ctcagaagaa ctcgtcaaga
2701 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag
2761 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc
2821 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt
2881 tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg
2941 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg
3001 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga
3061 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt
3121 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc
3181 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca
3241 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt
3301 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac
3361 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat
3421 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcataata
3481 ttattgaagc atttatcagg gttcgtctcg tcccggtctc ctcccatgca tg
//
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